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As a commemorative work of the five year anniversary of the Great Hanshin-Awaji earthquake, Hyogo Prefecture Government planned and executed a project called "Global Assessment of Earthquake Countermeasures". International and domestic experts were assigned to each of 20 items of problems related to the disaster. These experts visited Kobe for several days in 1999, discussed issues with the persons concerned, and collected data and evidences of the earthquake and the countermeasures used afterdoi:10.1017/s1049023x00031307 fatcat:muydrgsjprapjh26jxkaqirycu
more »... he disaster. On the theme "medical response", there apparently were many problems: search and rescue did not work well; ambulance services system failed; hospitals were damaged and overwhelmed; etc. Taking these precious lessons learned into account, several countermeasure projects were instituted during the last five years. In spite of the limited time and assistant manpower, the assigned experts analyzed and evaluated the progress of the countermeasure projects, identified areas of improvement, and noted the problems that still existed in the affected health/medical community. Details of the assessment of disaster countermeasures after the Great Hanshin-Awaji earthquake will be discussed in accordance with their report.
Research Institute for Information Science, Chubu University, Japan Takashi Fujii (email@example.com) -College of Engineering, Chubu University, Japan Masanori Kozawa (firstname.lastname@example.org) -College ...doi:10.1590/s1413-85572007000300006 fatcat:5na22tyjufhrljb23oxxvonleu
Combination of live-imaging and live-manipulation of developing embryos in vivo provides a useful tool to study developmental processes. Identification and selection of target cells for an in vivo livemanipulation are generally performed by experience-and knowledge-based decision-making of the observer. Computer-assisted live-prediction method would be an additional approach to facilitate the identification and selection of the appropriate target cells. Herein we report such a method usingdoi:10.1038/srep32962 pmid:27597656 pmcid:PMC5011986 fatcat:22z4vabaqnazjl6g7loc53asfa
more »... oping zebrafish embryos. We choose V2 neural progenitor cells in developing zebrafish embryo as their successive shape changes can be visualized in real-time in vivo. We developed a relatively simple mathematical method of describing cellular geometry of V2 cells to predict cell division-timing based on their successively changing shapes in vivo. Using quantitatively measured 4D live-imaging data, features of V2 cell-shape at each time point prior to division were extracted and a statistical model capturing the successive changes of the V2 cell-shape was developed. By applying sequential Bayesian inference method to the model, we successfully predicted division-timing of randomly selected individual V2 cells while the cell behavior was being live-imaged. This system could assist pre-selecting target cells desirable for real-time manipulation-thus, presenting a new opportunity for in vivo experimental systems. Real-time observation of developing embryos combined with in vivo live-manipulation provides useful opportunities for studying developmental processes 1-5 . The manipulation of target cells while live-imaging the embryo and studying the consequence could be used to determine the cause-effect relationship in development. For example, we previously manipulated cell shape by femtosecond laser and studied the causative effect of cellular eccentricity on cell fates 1 . The target cells for an in vivo live-manipulation suitable for the specific experimental objective are generally identified and selected by the observers based on their experience and knowledge. Such human skill-based approaches could be improved by the availability of a computer-assisted real-time prediction system to identify and select target cells for in vivo live-manipulation. To develop such a real-time prediction system, we chose developing zebrafish embryo as this organism provides an easily accessible model system for in vivo live-imaging and manipulation 3,5 . Using this model organism, we explored the possibility of developing a cell-shape based real-time prediction system. Cell-shape is a universal feature that is related to many biological processes and is a target of live-imaging and live-manipulations for the purpose of studying its relations to cell fates and functions in wide ranges of organisms 1,6-14 . Thus, a successful development of the real-time prediction system based on cell-shape could have a broad applicability in biology. Hence, we developed a real-time prediction method for division-timing of V2 neural progenitor cells (V2 cells) in developing zebrafish embryo based on their successively changing shapes. This model provides a suitable system to establish real-time prediction system based on cell-shape for the following two reasons: (1) the zebrafish line where individual V2 cells are labeled by green fluorescent protein is established 15 , making it easy to follow their behavior by live-imaging techniques; (2) V2 cells undergo successive shape changes before they divide to
療17: 671 -673, 1990 A CASE OF MALIGNANT TERATOMA OF THE ANTERIOR MEDIASTINUM PRODUCING ALPHA-FETOPROTEIN Naoyuki UMETANI, Kunihisa KOZAWA, Takashi INOUE, Takeyuki IDEI, Takashi WAKAHARA, Syuji NAKA, Yoshitsugu ...doi:10.3919/ringe1963.56.1338 fatcat:ugxh7xwao5fhfl552tru7tbb6y
A mechano-chemical reaction formed by a simple high-energy ball milling was applied to simulate the synthesis of ammonia and organic precursors from common inorganic materials that occurred on early Earth. By milling for 0.5–64 h at centrifugal accelerations of 20–150 G, ammonia and acetic acid were produced from inorganic materials, such as iron nitride, nitrate, carbide, and carbonates, in water. The experimental results can offer waste processing of metallic acids by the mechanical methoddoi:10.1515/gps-2018-0073 fatcat:fl2wrojeijglph4yzqdv6jx6na
more »... hout using any alkaline sources and suggest that the high-energy milling technology provides a new synthesis mode to form wide ranges of organic materials and scope for broader applications. This study also offers a new route to the formation of the precursors on early Earth and proposes that tremors and friction initiate micro-impacts between rocks and sand in the terrestrial crust, resulting in the formation of ammonia and organic materials from inorganic materials.
We previously proposed a bilevel quadrature-modulation (QM) envelope-pulse-width-modulation (EPWM) transmitter using half a side of a trilevel EPWM architecture. However, the optimum relationship between the modulation (DSM) sampling frequency (f s ), the interleaving frequency (f i ) and the carrier frequency (f c ) for a bilevel QM EPWM transmitter has not been investigated. This paper presents this optimum relationship, with the error vector magnitude (EVM) and the coding efficiency (CE) as figures of merit (FOMs).doi:10.2299/jsp.20.179 fatcat:n3j7kogrsfdptjb4mmiqctjslm
Previous studies have revealed that, at the initial step of carcinogenesis, transformed cells are often eliminated from epithelia via cell competition with the surrounding normal cells. In this study, we performed cell competition-based high-throughput screening for chemical compounds using cultured epithelial cells and confocal microscopy. PLX4720 was identified as a hit compound that promoted apical extrusion of RasV12-transformed cells surrounded by normal epithelial cells.doi:10.1016/j.isci.2020.101327 pmid:32688284 pmcid:PMC7371749 fatcat:moabz2r245fclczntvrz42yzk4
more »... of ZAK, a target of PLX4720, substantially enhanced the apical elimination of RasV12 cells in vitro and in vivo. ZAK negatively modulated the accumulation or activation of multiple cell competition regulators. Moreover, PLX4720 treatment promoted apical elimination of RasV12-transformed cells in vivo and suppressed the formation of potentially precancerous tumors. This is the first report demonstrating that a cell competition-promoting chemical drug facilitates apical elimination of transformed cells in vivo, providing a new dimension in cancer preventive medicine.
A small heat shock protein (HSP), HSP20 (HSPB6) is ubiquitously expressed in various tissues and has several functions. We previously reported that the expression of HSP20 protein in human hepatocellular carcinoma (HCC) cells is inversely proportional to the progression of HCC. In addition, we showed that HSP20 is associated with phosphoinositide 3-kinase (PI3K) and inhibits the proliferation of HCC cells via suppression of the AKT signaling pathway. However, the relationship between HSP20 anddoi:10.3892/or.2014.3278 pmid:24969689 fatcat:yivqmclrknc35g6v6immbibz7e
more »... poptosis in HCC has not yet been elucidated. To clarify whether HSP20 is implicated in the apoptosis of HCC cells, in the present study, we examined the effect of HSP20 on caspases, the central regulators of apoptosis, using human HCC-derived HuH7 cells that are transfected with wild-type human HSP20 (HSP20overexpressing cells). The cleavage of caspase-3 and caspase-7 in HSP20-overexpressing cells was enhanced compared with the empty vector-transfected cells (control cells). In addition, the cleavage of nuclear poly (ADP-ribose) polymerase (PARP) in HSP20-overexpressing cells was also strengthened. We further investigated the direct targets of HSP20 focusing on Bcl-2 family proteins in the HSP20-overexpressing cells. HSP20 proteins in the cells were coimmunoprecipitated with Bax. On the contrary, Bad, Bcl-2 and Bcl-xL were not coimmunoprecipitated with HSP20. These findings strongly suggest that HSP20 directly associates with Bax and stimulates caspase cascade in human HCC cells.
Yokochi a,b , Mitsunori Iwase c , and Kenji 5 Kozawa a . 6 a Health Support Center WELPO, Toyota Motor Corporation, 1-1 Ipponmatsu, Iwakura-cho, Toyota, 7 Aichi 444-2225, Japan. 8 b Midtown Clinic Meieki ... ; Toyota Motor Corporation, Health Support Center WELPO; Midtown Clinic Meieki Iwase, Mitsunori; Toyota Memorial Hospital Kozawa, Kenji; Toyota Motor Corporation, Health Support Center WELPO For peer review ...doi:10.1136/bmjopen-2017-019370 pmid:29306892 pmcid:PMC5781189 fatcat:xyyh3i7windqpbgk3o37bz42la
Transactions of the Atomic Energy Society of Japan
Many studies to clarify the mechanism, which hydrated cement maintains high pH condition for a long period have been reported. However, due to the lack of solid phase analysis, they do not related to the dissolution process of cement hydrates. We have performed a permeability test in which centrifugal force is used to percolate pore water and have studied the change of aqueous chemistries and that of the solid phase simultaneously in order to investigate the dissolution phenomena of hydrateddoi:10.3327/taesj2002.1.20 fatcat:iy6dpf5mprc7lkuko7peorggqy
more »... ent. The dissolution process of the hydrate phase agreed with that of the results of the other dissolution tests that is dissolving in the order of Na2O and K2O, Ca(OH)2 and C-S-H gel. Accordingly, it was ascertained that this permeability test was carried out under the equilibrium condition between percolated pore water and hydrates. It was confirmed that pore volume increased by the dissolving of hydrates and pore structure clearly changed. The relation between the volume of percolated water and its chemical composition was evaluated by the model coupling transport and chemical equilibrium calculations. The calculated calcium and silicon concentrations of the percolated water were in good agreement with the experimental values, and thus, we were considered that this model was useful for evaluating the behavior of cement hydrates dissolution.
Progress in Acute Abdominal Medicine
, Hiroshi Kozawa -233- 腹 部 救 急 診 療 の 進 歩 Vol.6. (2) 1986 図2 が 推 定 で き た31例 に つ い て み る と, 腹 腔 内 出 血 を生 じ た 例 は21例(67.7%)が 塊 状 型 で あ り, 以 下 多 結 節 型5例(16.1%), び 漫 型3例(9.7 %), 単 結 節 型2例(6.5%)の 順 で あ っ た 。 ... 2) 肝 細 胞 癌 のType及 び 大 き さ(図2) CT, US, 血 管 造 影 及 び剖 検 に よ り腫 瘍 の 形 態 【 索 引 用 語 】 肝 細 胞 癌, 腹 腔 内 出 血, TAE Department of Gastroenterology, Ogaki Municipal Hospital: Takashi Kumada, Satoshi Nakano, Isao Takeda ...doi:10.11231/jaem1984.6.233 fatcat:nlld5gj5tvdtvelrj4gixskebu
We have recently reported that short wavelength ultraviolet-C (UVC) irradiation inhibits cell growth and induces apoptosis in human pancreatic cancer cells. In this study, we investigated the effect of UVC on platelet-derived growth factor (PDGF)-BB-induced migration in pancreatic cancer cells, AsPC1 and BxPC3. In cell migration assays using a Boyden chamber Transwell, PDGF-BB exerted a maximum effect on migration of these cells at a dose of 70 ng/ml after 36 h of treatment. PDGF-BB also causeddoi:10.3892/or.2011.1612 pmid:22200892 pmcid:PMC3583433 fatcat:gpc3lbcowrer3gogq24qa3jcb4
more »... phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK), stress-activated protein kinase/c-Jun-N-terminal kinase (SAPK/JNK) and Akt, but not of p38 MAPK in these cells. Pretreatment of these cells with UVC at a dose over 10 J markedly suppressed PDGF-BB-induced migration. Since UVC significantly inhibited PDGF-BB-induced phosphorylation of Akt, and subsequent glycogen synthase kinase (GSK) 3β, but not p44/p42 MAPK and SAPK/JNK, it is likely that UVC inhibits PDGF-BB-induced migration by suppressing the Akt-GSK3β pathway in pancreatic cancer cells. Taken together with our previous findings, UVC could be a useful tool for the treatment of patients with pancreatic cancer.
Previous studies suggested that reduced muscular strength was one of the potential predictor of prevalence of diabetes mellitus. The purpose of this study was to investigate the association between toe flexor strength (TFS) and handgrip strength (HGS) and the prevalence of diabetes mellitus. Cross-sectional analysis was conducted using data from 1,390 Japanese males (35-59 years). TFS and HGS were measured and medical examinations undertaken. The prevalence of diabetes mellitus was defined asdoi:10.1507/endocrj.ej17-0517 pmid:29593144 fatcat:ut4doyyzg5b3vnhdamp4gy3c6m
more »... sting blood glucose ≥126 mg/dL, glycated hemoglobin ≥6.5% (48 mmol/mol), and/or current use of anti-diabetes mellitus drugs. A total of 114 participants had diabetes mellitus. TFS in participants with diabetes mellitus was significantly lower than that in persons not suffering from diabetes mellitus but HGS was not. Odds ratio (OR) and 95% confidence interval (CI) per 1-standard deviation-increase in muscular strength measurements for the prevalence of diabetes mellitus were obtained using a multiple logistic regression model. Prevalence of diabetes mellitus was inversely related to TFS (OR 0.769, 95% CI 0.614-0.963), TFS/body mass (BM) (0.696, 0.545-0.889) and TFS/body mass index (BMI) (0.690, 0.539-0.882) after adjustment of covariates. Such associations were not observed in HGS (OR 0.976, 95% CI 0.773-1.232), HGS/BM (0.868, 0.666-1.133) or HGS/BMI (0.826, 0.642-1.062). These results suggested that poor TFS was associated with an increased prevalence of diabetes mellitus independent of visceral fat accumulation, but HGS was not, in middle-aged males. TFS may be a better marker for the prevalence of diabetes mellitus than HGS.
Platinum-containing anti-cancer drugs such as cisplatin are widely used for patients with various types of cancers, however, resistance to cisplatin is observed in some cases. Whereas we have recently reported that high dose UV-C (200 J/m²) induces colorectal cancer cell proliferation by desensitization of EGFR, which leads oncogenic signaling in these cells, in this study we investigated the combination effect of low dose cisplatin (10 μM) and low dose UV-C (10 J/m²) on cell growth anddoi:10.1186/1476-4598-11-45 pmid:22788833 pmcid:PMC3477093 fatcat:i6nk62l4fzhk3i3nkf5pgvysmi
more »... s in several human colorectal cancer cells, SW480, DLD-1, HT29 and HCT116. Results: The combination inhibited cell cycle and colony formation, while either cisplatin or UV-C alone had little effect. The combination also induced apoptosis in these cells. In addition, the combination caused the downregulation of EGFR and HER2. Moreover, UV-C alone caused the transient internalization of the EGFR, but with time EGFR recycled back to the cell surface, while cisplatin did not affect its localization. Surprisingly, the combination caused persistent internalization of the EGFR, which results in the lasting downregulation of the EGFR. Conclusions: The combination of low dose cisplatin and low dose UV-C synergistically exerted anti-cancer effect by down-regulating RTK, such as EGFR and HER2. These findings may provide a novel strategy for the treatment of patients with colorectal cancer.
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