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The most abundant nascent poly(A)+RNAs are transcribed by RNA polymerase III in murine tumor cells

D.A. Kramerov, S.V. Tillib, G.P. Shumyatsky, G.P. Georgiev
1990 Nucleic Acids Research  
Twelve to twenty percent of newly synthesized poly(A)+RNA is transcribed by RNA polymerase Ill in Ehrlich ascites carcinoma and P301 plasmocytoma mouse tumors.  ...  Several transcripts that hybridize with B2 probe were also observed in poly(A)-RNA. The major components consist of 180, 160, 120 and 95 nucleotides.  ...  ACKNOWLEDGEMENT We thank I.N.Strizhak for typing of the manuscript.  ... 
doi:10.1093/nar/18.15.4499 pmid:1697065 pmcid:PMC331270 fatcat:uzmmpv3wk5arjeeeqnv6xoryrm

Isolation and characterization of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole-resistant mutants of the Chinese hamster ovary cell line

V L Funanage
1982 Molecular and Cellular Biology  
Whereas DRB inhibited RNA polymerase II activity in WTCHO cells by a maximum of 60% at concentrations as low as 60 microM, 300 microM DRB was required to inhibit 60% of the RNA polymerase II activity in  ...  Somatic cell hybrids of DRB-resistant and alpha-amanitin-resistant cell lines grew in medium containing both DRB and alpha-amanitin, demonstrating that the drbR and amaR mutations were not in the same  ...  Clark, in whose laboratory this work was performed, for his sponsorship and support. Some of the mutagenesis experiments were performed while I was a visiting researcher at Haskell Laboratory, E. I.  ... 
doi:10.1128/mcb.2.4.467 pmid:6180307 pmcid:PMC369811 fatcat:x5o7nyrt5beddoqlfo2ylgknzm

Ongoing activity of RNA polymerase II confers preferential repair of nitrogen mustard-induced N-alkylpurines in the hamster dihydrofolate reductase gene

K Wassermann, J Damgaard
1994 Cancer Research  
Nuclear run-off transcription analysis verified that alpha-amanitin completely and selectively inhibited transcription by RNA polymerase II.  ...  By treating cells or not with the transcription inhibitor alpha-amanitin, we have explored whether ongoing activity of RNA polymerase II was coordinately related to proficient repair of nitrogen mustard-induced  ...  lower average molecular weight (lanes C and D).  ... 
pmid:7505196 fatcat:uojtrj2dbvap7f4cm2q2x53tme


C. Kedinger
1974 Journal of Cell Biology  
Our autoradiographic and biochemical evidence indicates that in CHO cells high molecular weight extranucleolar RNA synthesis (HnRNA) is blocked by the a-amanitin treatment, whereas nucleolar RNA (preribosomal  ...  a-Amanitin acts in vitro as a selective inhibitor of the nucleoplasmic form B RNA polymerases.  ...  unaffected, whereas extranucleolar high molecular weight RNA synthesis is strongly inhibited by a-amanitin action.  ... 
doi:10.1083/jcb.63.3.831 pmid:4474178 pmcid:PMC2109362 fatcat:zddmbtoltjgb7n6st3fc7r5gt4

Nucleotide polymerases in the developing avian erythrocyte

S S Longacre, W J Rutter
1977 Journal of Biological Chemistry  
Three nucleotide polymerase types were investigated: (a) DNA-dependent RNA polymerases; (b) RNA-dependent terminal ribonucleotidyltransferases, and (c) DNA-dependent DNA polymerases.  ...  The two characteristic forms of eucaryotic DNA-dependent RNA polymerases, polymerase I (nucleolar) and polymerase II (nucleoplasmic), were identified.  ...  Fred Bollum particularly for his helpful suggestions and discussions and for his generous gifts of poly(dT), poly(dC), and (rA,),.  ... 
pmid:833121 fatcat:dnq4zd45mra43f7l4gzd4o6ese

The Structure and Replication of Coronaviruses [chapter]

St. Siddell, H. Wege, V. ter Meulen
1982 Current Topics in Microbiology and Immunology  
The authors are supported by the Deutsche Forschungsgemeinschaft and would like to thank Helga Kriesinger for typing the manuscript.  ...  The virus did, however, grow in an alpha-amanitin-resistant Chinese hamster ovary (CHO) cell line in the presence of the drug.  ...  A number of experiments indicate the topography of the low molecular weight glycoprotein in the virion.  ... 
doi:10.1007/978-3-642-68528-6_4 fatcat:ysnukni2qbby5d4boaoxifsp7e

Subject Index

1976 Proceedings of the National Academy of Sciences of the United States of America  
enzymes by a factor of low molecular weight abundant in some leukemic cells, 77 Regulation of induction of T and B cell colonies in vitro by normal human lymphocytes, 4546 ca-Glucosyltransferase see Dextrin  ...  , 4105 Crystallin Absence of low-molecular-weight alpha crystallin in nuclear region of old human lenses, 3484 Quantitation of b-crystallin messenger RNA during lens induction in chick embryos, 2808  ...  Rohon-Beard cell  ... 
doi:10.1073/pnas.73.12.4715 fatcat:myymdsndmjcobl4ovjmnz3avae

Descriptions of Virus Families and Groups [chapter]

R. I. B. Francki, C. M. Fauquet, D. L. Knudson, F. Brown
1991 Archives of virology. Supplementum  
The Families and Groups Derivation of Names  ...  Virus- specific messenger RNA synthesis is inhibited by actinomycin D or a-amanitin due to blockage of host DNA-dependent transcription and a presumed lack of newly synthesized substrate for viral endonuclease  ...  protein(s) and a large molecular weight polymerase protein, plus in paramyxoviruses several non-structural proteins of unknown function.  ... 
doi:10.1007/978-3-7091-9163-7_8 fatcat:c4le7xtw5rfk3k7pt3qxty3xjq

C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system

2019 Nucleic Acids Research  
transcripts in host-cell cytoplasm by means of two components.  ...  First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase.  ...  ACKNOWLEDGEMENTS We thank the French Ministry of Research and Innovation and BpiFrance for their encouragements and financial support.  ... 
doi:10.1093/nar/gkz069 pmid:30726994 pmcid:PMC6412113 fatcat:ndmgej6rizcbnodv5kx2j62wwu

The Viruses [chapter]

Frederick A. Murphy, Claude M. Fauquet, David H. L. Bishop, Said A. Ghabrial, Audrey W. Jarvis, Giovanni P. Martelli, Mike A. Mayo, Max D. Summers
1995 Virus Taxonomy  
A glossary of abbreviations and terms is provided first; followed by a set of virus diagrams and listings of the taxa, alphabetically, then by host, and then by nucleic acid and genome characteristics.  ...  A key to the placement of the viruses in the taxa is provided. Descriptions of the taxa and a listing of unassigned viruses follow.  ...  Early genes are transcribed by host RNA polymerase II, while late and very late genes are transcribed by an alpha-amanitin resistant RNA polymerase activity.  ... 
doi:10.1007/978-3-7091-6607-9_2 fatcat:ckrv7rscwrenvekrrmwvdoarai

Master subject index

1977 FEBS Letters  
retinal pigment epithelium effect on synthesis of small molecular weight RNA D-Amino acid oxidase Amino acid sequence analysis on solid phase, measurement of coupling capacity use of phenylene  ...  Tubulin, radioimmunoassay Turnip yellow mosaic virus (TYMV) RNA, translation in high molecular weight proteins Tween 20, use in membrane protein extraction of Acholeplasma Ubiquinone <ytochrome c reductase  ... 
doi:10.1016/0014-5793(77)80912-5 fatcat:cgljib5ggvai5bw6usvuy3hx3y

Abstracts of Papers Presented at the Session of the Virology Section of the Deutsche Gesellschaft für Hygiene und Mikrobiologie, Marburg, February 23-25, 1989

1989 Zentralblatt für Bakteriologie  
Extracts from insect cells infected with tkl-recombinant baculovirus were incubated with antibodies raised against pS1 c-tkl and a protein kinase assay was performed.  ...  Under these experimental conditions PS1 c-tkl shows autophosphorylation activity. -Furthermore we examined the expression of c-tkl related genes in human tissues and corresponding tumors.  ...  -Alpha-Amanitin showed a weak inhibitory effect in the highest nontoxic concentration of 0.1 Ilg/ml. Acyclovir had no inhibitory effect on HOV RNA replication.  ... 
doi:10.1016/s0934-8840(89)80038-6 fatcat:jnrkflvr5bdsrgeqnek6at3pja

Radiation vs Nuclear Size [chapter]

2008 Encyclopedia of Genetics, Genomics, Proteomics and Informatics  
It is comparable to molecular weight in daltons but it is not identical to molecular weight (MW) represented by the mass of atoms involved.  ...  I Relative Molecular Mass (M r ): Expresses molecular weight relative to 12 C isotope (in 1 = 12 units).  ...  In the haploid yeast cell there are 2,000 to 4,000 RNA polymerase II molecules and about ten times more general transcription factor molecules. In 2006, Roger D.  ... 
doi:10.1007/978-1-4020-6754-9_14032 fatcat:sjsarml6jbh37bxjg25tyvucga

Df31 Protein and snoRNAs Maintain Accessible Higher-Order Structures of Chromatin

Thomas Schubert, Miriam Caroline Pusch, Sarah Diermeier, Vladimir Benes, Elisabeth Kremmer, Axel Imhof, Gernot Längst
2012 Molecular Cell  
Several factors are implicated in regulatory chromatin loop formation, like insulator proteins, transcription factors, polycomb proteins, chromatin remodelling factors and architectural proteins (Cai et  ...  Chromatin loops are frequently formed between the chromatin binding sites of these factors. Some studies suppose that chromatin  ...  Depletion of RNA, either by RNaseA or Polymerase II inhibition by alpha amanitin treatment, resulted in a genome wide decrease of chromatin accessibility in vivo (section 4.1.).  ... 
doi:10.1016/j.molcel.2012.08.021 pmid:23022379 fatcat:wfulzedc45a2lp3stwrsfmngyi

The alphaviruses: gene expression, replication, and evolution

J H Strauss, E G Strauss
1994 Microbiological reviews  
The interactions of the viruses with host cells and host organisms have been exclusively studied, and the molecular basis of virulence and recovery from viral infection have been addressed in a large number  ...  The alphaviruses are a genus of 26 enveloped viruses that cause disease in humans and domestic animals. Mosquitoes or other hematophagous arthropods serve as vectors for these viruses.  ...  The alpha-like viruses all have a polymerase of group 3 in the nomenclature of Koonin, the picorna-like viruses have a polymerase of group 1, and the flaviviruses and their plant relatives (carmoviruses  ... 
pmid:7968923 pmcid:PMC372977 fatcat:f5brpseg2nawjnbzq6oskpf7ne
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