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Pumiliotoxin metabolism and molecular physiology in a poison frog [article]

Aurora Alvarez-Buylla, Cheyenne Y Payne, Charles Vidoudez, Sunia A Trauger, Lauren A O'Connell
2020 bioRxiv   pre-print
We performed a feeding experiment with the Dyeing poison frog (Dendrobates tinctorius) to ask if this species can metabolize PTX 251D into aPTX 267A and what gene expression changes are associated with  ...  (A) Frogs were orally 151 administered either DHQ or DHQ+PTX once a day for five days.  ...  A generalized linear mixed 124 model was used (glmmTMB package in R) [20] to test for differences in alkaloid abundance 125 across tissues and treatment type, with the frog as a random effect and a negative  ... 
doi:10.1101/2020.11.03.367524 fatcat:w7pn5a5gbzf3jmo5fycby7653q

Peptide and protein analysis with mass spectrometry

Sunia A. Trauger, William Webb, Gary Siuzdak
2002 Spectroscopy (Ottawa)  
Mass spectrometry (MS) is rapidly becoming a fundamental tool for biologists and biochemists in their efforts to characterize cellular function.  ...  Sample preparatory and cleanup techniques used in our laboratory for protein and peptide analysis are provided, along with a discussion of data interpretation.  ...  a minute.  ... 
doi:10.1155/2002/320152 fatcat:cxggxfmjtvajdimdp5ueowviwm

Rapid toxin sequestration impacts poison frog physiology [article]

Lauren A O'Connell, Jeremy D O'Connell, Joao A Paulo, Sunia A Trauger, Steven P Gygi, Andrew W Murray, LS50: Integrated Science Laboratory Course
2020 bioRxiv   pre-print
A pool of each control sample was labelled collectively to create a bridge in an additional channel.  ...  A generalized linear mixed model showed a significant effect of group (p=5.163x10 -5 ) and tissue (p=0.001).  ... 
doi:10.1101/2020.05.27.119081 fatcat:yb3ppxnao5boxjubmpom72ub3y

Microstructures amplify seemingly honest signals in carotenoid-colored tanagers [article]

Dakota E. McCoy, Allison J. Shultz, Charles Vidoudez, Emma van der Heide, Sunia A. Trauger, David Haig
2019 bioRxiv   pre-print
Together, our results suggest that a widely cited index of honesty - carotenoid pigments - cannot fully explain male appearance.  ...  Males and females within a species have equivalent amounts and types of carotenoids, which is surprising if carotenoids are an honest-because-costly signal.  ...  Male 458 carotenoids are thus a signal of a functioning metabolism.  ... 
doi:10.1101/799783 fatcat:hcfqbtavwjglvjaxc4lqcvwgmm

Quantitative ESI-TOF Analysis of Macromolecular Assembly Kinetics

Anne E. Bunner, Sunia A. Trauger, Gary Siuzdak, James R. Williamson
2008 Analytical Chemistry  
The Escherichia coli small (30S) ribosomal subunit is a particularly well-characterized model system for studying in vitro self-assembly.  ...  A previously developed pulse-chase monitored by quantitative mass spectrometry (PC/QMS) approach to measuring kinetics of in vitro 30S assembly suffered from poor signal-to-noise and was unable to observe  ...  The peak fitting procedure provides A U , the amplitude of the unlabeled peak, and A L , the amplitude of the 15 N-labeled peak. The fraction of 15 N protein is given by f L ) A L /(A L + A U ).  ... 
doi:10.1021/ac8020505 pmid:19007188 pmcid:PMC2735594 fatcat:ebyp34nvl5hvvi5jbaqpelrf34

Investigating Viral Proteins and Intact Viruses with Mass Spectrometry [chapter]

Sunia A. Trauger, Teri Junker, Gary Siuzdak
2003 Topics in current chemistry  
Mass spectrometry has become a fundamental technology for virology and an important tool in probing the structure and function of viruses.  ...  Trauger et al. Fig. 7 . Top: mass spectra of FHV g-peptide after acetylation.  ...  Trauger et al. 5 . The approach to probing higher order protein structure by mass spectrometry is shown.  ... 
doi:10.1007/3-540-36113-8_8 fatcat:3r2f4fwmarashhralqdwsnzkz4

The anticancer natural product ophiobolin A induces cytotoxicity by covalent modification of phosphatidylethanolamine

Christopher Chidley, Sunia A Trauger, Kıvanç Birsoy, Erin K O'Shea
2016 eLife  
Here, we used a loss-of-function genetic screen in human haploid KBM7 cells to discover the mechanism of action of the anticancer natural product ophiobolin A (OPA).  ...  We thank A Darnell for critical reading of the manuscript. Additional information  ...  An example of such a natural product is ophiobolin A (OPA), a plant toxin isolated from pathogenic fungi of the Bipolaris genus which displays cytotoxicity at nanomolar concentrations against a range of  ... 
doi:10.7554/elife.14601 pmid:27403889 pmcid:PMC4942256 fatcat:ddwxu2g3ezgqrjeptutny5b244

Mechanisms of convergent egg-provisioning in poison frogs [article]

Eva K Fischer, Alexandre B Roland, Nora A Moskowitz, Charles Vidoudez, Ndimbintsoa Ranaivorazo, Elicio E Tapia, Sunia A Trauger, Miguel Vences, Luis A Coloma, Lauren A O'Connell
2019 bioRxiv   pre-print
In contrast at a molecular level, only mantellids showed increased oxytocin neuron activity akin to that in nursing mammals.  ...  Samples were run on a Thermo Q-Exactive Plus and a Phenomenex Gemini C18 3 μm 153 2.1 × 100 mm column (Torrance, CA, USA).  ...  Fluorescent sections were imaged on a Leica 205 DM4B compound light microscope connected to a fluorescent light source.  ... 
doi:10.1101/653501 fatcat:iyw5sjhp6rgfhjn4fzsionuvfi

How the glycosyltransferase OGT catalyzes amide bond cleavage

John Janetzko, Sunia A Trauger, Michael B Lazarus, Suzanne Walker
2016 Nature Chemical Biology  
OGT is involved in HCF-1 processing raised questions about how a glycosyltransferase might promote amide bond hydrolysis.  ...  Here we show that HCF-1 cleavage occurs via glycosylation of a glutamate side chain followed by on-enzyme formation of an internal pyroglutamate, which undergoes spontaneous backbone hydrolysis.  ...  Funding for this work was provided by a National Institutes of Health grant (R01 GM094263) to SW.  ... 
doi:10.1038/nchembio.2173 pmid:27618188 pmcid:PMC5172607 fatcat:blv35pmzevgjvk7hdfndmln67y

Catalysis of cataract-associated human γD crystallin aggregation via dynamic disulfide exchange [article]

Eugene Serebryany, Shuhuai Yu, Sunia A. Trauger, Bogdan Budnik, Eugene I. Shakhnovich
2018 bioRxiv   pre-print
The search for a biochemical mechanism of catalysis of W42Q aggregation by WT has revealed that WT HγD can transfer a disulfide bond to the W42Q variant.  ...  The aggregating variant thus becomes a disulfide sink, removing the disulfides from solution.  ...  Jonathan A. King for mentorship and discussion at the early stages of this 688 research. We thank Renee Robinson for technical assistance with mass spectrometry. 689 690  ... 
doi:10.1101/300608 fatcat:s26yjsh6dfhmtfzme3bpxmkz6y

Microstructures amplify carotenoid plumage signals in tanagers

Dakota E. McCoy, Allison J. Shultz, Charles Vidoudez, Emma van der Heide, Jacqueline E. Dall, Sunia A. Trauger, David Haig
2021 Scientific Reports  
AbstractBrilliantly-colored birds are a model system for research into evolution and sexual selection.  ...  Our results suggest that a widely cited index of honesty, carotenoid pigments, cannot fully explain male appearance.  ...  This work was performed in part at the Harvard University Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the  ... 
doi:10.1038/s41598-021-88106-w pmid:33883641 pmcid:PMC8060279 fatcat:jfe4dc36jnbp5kjvaogpbzeoju

Hydrogen peroxide as a hydride donor and reductant under biologically relevant conditions

Yamin Htet, Zhuomin Lu, Sunia A. Trauger, Andrew G. Tennyson
2019 Chemical Science  
Hydrogen peroxide functioned as a hydride donor and terminal reductant for the ruthenium-catalyzed reduction of radicals under biologically-relevant conditions.  ...  We thank A. Mangalum for prior work with Ru1 and helpful discussions. Notes and references  ...  the 2e À for reducing 2 equiv. of ABTSc À , which would most likely occur in the form of a hydride (H À ).  ... 
doi:10.1039/c8sc05418e pmid:30881631 pmcid:PMC6381410 fatcat:ujph5xcgcrbq5hw33jqekfltli

Molecular physiology of chemical defenses in a poison frog [article]

Stephanie N. Caty, Aurora Alvarez-Buylla, Gary D. Byrd, Charles Vidoudez, Alexandre B. Roland, Elicio E. Tapia, Bogdan Bodnik, Sunia A. Trauger, Luis A. Coloma, Lauren A. O'Connell
2019 bioRxiv   pre-print
Para entender cómo las ranas venenosas mueven los alcaloides de su dieta a las glándulas granulares en su piel, nos enfocamos en medir la expresión de genes en tres tejidos: intestinos, piel e hígado.  ...  alkaloids influences frog physiology and that small molecule transport proteins may be involved in toxin bioaccumulation in dendrobatid poison frogs.ResumenLas ranas venenosas obtienen moléculas lipofílicas a  ...  A representative extracted ion 166 chromatograms for each group was normalized to the same scale to create a visual overview.  ... 
doi:10.1101/591115 fatcat:a6mzn2o5qjgg5e6az6eil6fnu4

A Metalloantibody That Irreversibly Binds a Protein Antigen

Kirk Trisler, Loren L. Looger, Vikram Sharma, Martin Baker, David E. Benson, Sunia Trauger, Peter G. Schultz, Vaughn V. Smider
2007 Journal of Biological Chemistry  
Here we report the design and construction of an antibody which forms an irreversible complex with a protein antigen in a metal dependent reaction.  ...  To cross this affinity threshold, antibodies which tether their targets in a manner other than reversible non-covalent interaction will be required.  ...  A. Effect of EDTA.  ... 
doi:10.1074/jbc.m704675200 pmid:17617633 fatcat:b7uknqctnjaobks44wvfyedoqm

Assignment of Endogenous Substrates to Enzymes by Global Metabolite Profiling†

Alan Saghatelian, Sunia A. Trauger, Elizabeth J. Want, Edward G. Hawkins, Gary Siuzdak, Benjamin F. Cravatt
2004 Biochemistry  
method to discover several brain lipids regulated by the mammalian enzyme fatty acid amide hydrolase (FAAH) in ViVo, including known signaling molecules (e.g., the endogenous cannabinoid anandamide) and a  ...  Here, we describe a general strategy for identifying endogenous substrates of enzymes by untargeted liquid chromatography-mass spectrometry (LC-MS) analysis of tissue metabolomes from wild-type and enzymeinactivated  ...  SUPPORTING INFORMATION AVAILABLE Tables of ion intensities, a table of exact mass data for C22:0, C24:1, and C24:0, and three figures depicting MS/ MS spectra of compounds from FAAH(-/-) mice, the acyl  ... 
doi:10.1021/bi0480335 pmid:15533037 fatcat:6h6qqwvyifcozp3n77rdy4czfq
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