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Subcellular protein expression models for microsatellite instability in colorectal adenocarcinoma tissue images

Violeta N. Kovacheva, Nasir M. Rajpoot
2016 BMC Bioinformatics  
We then develop models for six proteins which are important colorectal cancer biomarkers and are associated with microsatellite instability, namely MLH1, PMS2, MSH2, MSH6, P53 and PTEN.  ...  To the best of our knowledge, this is the first model for expression of multiple proteins in anatomically intact tissue, rather than within cells in culture.  ...  To the best of our knowledge, this is the first model for subcellular expression of multiple proteins in anatomically intact tissue, as opposed to existing models for protein expression within cells in  ... 
doi:10.1186/s12859-016-1243-y pmid:27770786 pmcid:PMC5075203 fatcat:w37ihtnke5enxegu3gdh2gxjyi

MYO1A (myosin IA)

del Corro D Arango, R Mazzolini
2014 Atlas of Genetics and Cytogenetics in Oncology and Haematology  
Review on MYO1A, with data on DNA/RNA, on the protein encoded and where the gene is This article should be referenced as such:  ...  (C) Localization of additional mutations found in colorectal tumors without microsatellite instability.  ...  Mazzolini et al. reported MYO1A frame-shift mutations in 32% (37 of 116) of the colorectal tumors with microsatellite instability.  ... 
doi:10.4267/2042/54010 fatcat:34qhult25febjjcxqiq5s4b5w4

Differential expression of DHHC9 in microsatellite stable and instable human colorectal cancer subgroups

F Mansilla, K Birkenkamp-Demtroder, M Kruhøffer, F B Sørensen, C L Andersen, P Laiho, L A Aaltonen, H W Verspaget, T F Ørntoft
2007 British Journal of Cancer  
The upregulation was more striking in microsatellite stable (MSS), than in microsatellite instable (MSI), tumours.  ...  In conclusion, DHHC9 is a gastrointestinal-related protein highly expressed in MSS colon tumours.  ...  ACKNOWLEDGEMENTS We thank Pamela Celis, Susanne Bruun and Jette Jensen for their excellent technical assistance as well as to Jeppe Praetorius, Institute of Anatomy, University of Aarhus, for confocal  ... 
doi:10.1038/sj.bjc.6603818 pmid:17519897 pmcid:PMC2359975 fatcat:4uqqytt36bdutogppbof262fgu

Parkin protein expression and its impact on survival of patients with advanced colorectal cancer

Caroline Veloso da S Claudia, Kuhl Svoboda Baldin Rosimeri, Louise Costacurta Po Nayanne, Pereira Agostinho Amanda, Olandosk Marcia, de Noronha Lúcia, Santos Sotomaior Vanessa
2018 Cancer Biology and Medicine  
parameters, and expression of known proteins involved in colorectal cancer.  ...  Although alterations in parkin protein expression have been described in colorectal cancer, the functional significance of this protein remains unknown.  ...  non-neoplastic mucosa 0.409 1.04 0.94-1.15 Table S1 S1 Immunohistochemical analysis of microsatellite instability markers expression in colorectal cancer patients Protein Positive/negative  ... 
doi:10.20892/j.issn.2095-3941.2017.0136 pmid:29545969 pmcid:PMC5842336 fatcat:fjxkatrllrb6hao4yfrptx7rrm

MSH2missense mutations and HNPCC syndrome: pathogenicity assessment in a human expression system

Laura Belvederesi, Francesca Bianchi, Eva Galizia, Cristian Loretelli, Raffaella Bracci, Romina Catalani, Monica Amati, Riccardo Cellerino
2008 Human Mutation  
Functional assays results were correlated with clinical and genetic features indicative of HNPCC as MicroSatellite-Instability (MSI), abnormalities of MMR gene expression in tumour tissue (IHC) and familial  ...  Missense alterations, were assessed in a human system for expression/stability and for the ability to heterodimerize with MSH6 and correctly localize into the nucleus.  ...  Index patient MSI analysis resulted in a high degree of instability (80%). Normal staining for MLH1 and the lack of MSH2 and MSH6 expression were observed in the tumour tissue.  ... 
doi:10.1002/humu.20875 pmid:18781619 fatcat:xdzhaohd3vfzppg5yuhgfjt7y4

Phosphoprotein Keratin 23 accumulates in MSS but not MSI colon cancers in vivo and impacts viability and proliferation in vitro

Karin Birkenkamp-Demtroder, Francisco Mansilla, Flemming Brandt Sørensen, Mogens Kruhøffer, Teresa Cabezón, Lise Lotte Christensen, Lauri A. Aaltonen, Hein W. Verspaget, Torben Falck Ørntoft
2007 Molecular Oncology  
In conclusion, Keratin23 expression is a novel and important difference between microsatellite-stable and microsatellite-instable colon cancers.  ...  Immunohistochemical analyses demonstrated that 88% of microsatellite-instable tumors were negative for Keratin23 protein, while 70% of MSS tumors and metastases derived from MSS-tumors showed high Keratin23  ...  Acknowledgments We are grateful to Pamela Celis and Susanne Bruun for their excellent technical assistance and Jeppe Praetorius, Institute of Anatomy, University of Aarhus, for confocal microscopy.  ... 
doi:10.1016/j.molonc.2007.05.005 pmid:19383294 pmcid:PMC5543893 fatcat:zwc6lzvcbfge5otzicdfcipblq

Brush border Myosin Ia has tumor suppressor activity in the intestine

R. Mazzolini, H. Dopeso, S. Mateo-Lozano, W. Chang, P. Rodrigues, S. Bazzocco, H. Alazzouzi, S. Landolfi, J. Hernandez-Losa, E. Andretta, P. Alhopuro, E. Espin (+10 others)
2012 Proceedings of the National Academy of Sciences of the United States of America  
MYO1A frame-shift mutations were observed in 32% (37 of 116) of the colorectal tumors with microsatellite instability analyzed, and evidence of promoter methylation was observed in a significant proportion  ...  Moreover, MYO1A tumor expression was found to be an independent prognostic factor for colorectal cancer patients.  ...  Results Frequent MYO1A Mutations in Colorectal Tumors with Microsatellite Instability.  ... 
doi:10.1073/pnas.1108411109 pmid:22307608 pmcid:PMC3277176 fatcat:lccbdaupnjdwfj66uoszr6hfey

Sequential DNA methylation changes are associated with DNMT3B overexpression in colorectal neoplastic progression

A. E. K. Ibrahim, M. J. Arends, A.-L. Silva, A. H. Wyllie, L. Greger, Y. Ito, S. L. Vowler, T. H.-M. Huang, S. Tavare, A. Murrell, J. D. Brenton
2010 Gut  
Tissue microarrays from a subset of 64 cases were used for immunohistochemical analysis of DNMT3B expression.  ...  and aims Although aberrant methylation of key genes in the progression of colorectal neoplasia has been reported, no model-based analysis of the incremental changes through the intermediate adenoma stage  ...  We also acknowledge the help of the Tissue Bank facility in Addenbrooke's hospital funded through the NIHR Biomedical Research Centre.  ... 
doi:10.1136/gut.2010.223602 pmid:21068132 fatcat:tt464vbkb5anlo6gzoparzbvva

Tumor-Associated Alterations in Caspase-14 Expression in Epithelial Malignancies

M. Krajewska
2005 Clinical Cancer Research  
Experimental Design: Tumor-associated alterations in caspase-14 expression were observed for cervical, ovarian, breast, gastric, and colon cancers.  ...  Conclusions: The findings reveal tumor-specific alterations in caspase-14 expression and suggest that differences in its expression may define subsets of epithelial cancers with distinct clinical behaviors  ...  Guy Salvesen from the Burnham Institute, La Jolla, CA for providing the caspase-14 recombinant protein for antibodies production and Wei-Soon Kwon for technical assistance.  ... 
doi:10.1158/1078-0432.ccr-04-2527 pmid:16061862 fatcat:6lrbxlbnlvdxvbcvz4d6ppjo2i

Expression of ERalpha, ERbeta and co-regulator PELP1/MNAR in colorectal cancer: prognostic significance and clinicopathologic correlations

Petros D Grivas, Vassiliki Tzelepi, Georgia Sotiropoulou-Bonikou, Zinovia Kefalopoulou, Athanasios G Papavassiliou, Haralabos Kalofonos
2009 Cellular Oncology  
ERalpha, ERbeta and PELP1/MNAR protein expression were evaluated by immunohistochemistry in colorectal normal mucosa, adenomas and adenocarcinomas from 113 patients with colorectal cancer.  ...  Estrogen receptor beta (ERbeta) is abundantly expressed in colorectal tissue, but its role in colorectal carcinogenesis remains elusive.  ...  Study sponsors had no involvement in the study design, in the collection, analysis and interpretation of data; in the writing of the manuscript; in the decision to submit the manuscript for publication  ... 
doi:10.3233/clo-2009-0467 pmid:19478391 pmcid:PMC4618984 fatcat:fruybzmoxfbsbaxyq5vd63xxle

Interplay between SOX9, β-catenin and PPARγ activation in colorectal cancer

Anna Panza, Valerio Pazienza, Maria Ripoli, Giorgia Benegiamo, Annamaria Gentile, Maria Rosaria Valvano, Bartolomeo Augello, Giuseppe Merla, Clelia Prattichizzo, Francesca Tavano, Elena Ranieri, Pierluigi di Sebastiano (+4 others)
2013 BBA - Molecular Cell Research  
tumor tissue and age (p = 0.04) and microsatellite instability (MSI), in particular with MSI-H (p = 0.0002).  ...  In CRC tissues SOX9 resulted up-regulated at both mRNA and protein levels when compared to matched normal mucosa, β-catenin resulted up-regulated at protein levels, while PPARG mRNA and PPARγ protein levels  ...  Stefano Ferrari for excellent support in the cell-cycle experiment, and Dr. Rosa Di Paola and Dr. Antonella Marucci for technical contribution.  ... 
doi:10.1016/j.bbamcr.2013.04.004 pmid:23583560 fatcat:niime4mkhfdlvgbg6yo2va5nby

Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling

Xiang Hu, Long Zhang, Yaqi Li, Xiaoji Ma, Weixing Dai, Xiaoxue Gao, Xinxin Rao, Guoxiang Fu, Renjie Wang, Mengxue Pan, Qiang Guo, Xiaoya Xu (+6 others)
2020 EBioMedicine  
Dachshund homologue 1 (DACH1) is highly expressed in LGR5+ intestinal stem cells and colorectal tumours.  ...  We used immunohistochemistry, western blotting and quantitative real-time PCR to analyse DACH1 expression in colorectal cancer (CRC) samples.  ...  ; MSS, microsatellite stability; MSI, microsatellite instability.  ... 
doi:10.1016/j.ebiom.2020.102800 pmid:32512510 pmcid:PMC7281795 fatcat:mg4hyvguzfcifcb6qncope3rjq

Functions of the APC tumor suppressor protein dependent and independent of canonical WNT signaling: implications for therapeutic targeting

William Hankey, Wendy L. Frankel, Joanna Groden
2018 Cancer Metastasis Review  
APC encodes a 312-kDa protein that localizes to multiple subcellular compartments and performs diverse functions.  ...  Individuals with FAP inherit a loss-of-function mutation in a single allele of APC, followed by an additional acquired mutation in the second allele of APC in the adenomas and adenocarcinomas that develop  ...  responsible for normal homeostasis of the colorectal epithelium and other tissues [88, 148] .  ... 
doi:10.1007/s10555-017-9725-6 pmid:29318445 pmcid:PMC5803335 fatcat:arnxpedybfcptngwx6qpfa7574

Molecular Pathogenesis of Colorectal Cancer with an Emphasis on Recent Advances in Biomarkers, as Well as Nanotechnology-Based Diagnostic and Therapeutic Approaches

Fakhria A. Al-Joufi, Aseem Setia, Mounir M. Salem-Bekhit, Ram Kumar Sahu, Fulwah Y. Alqahtani, Retno Widyowati, Fadilah Sfouq Aleanizy
2022 Nanomaterials  
The formation of colorectal adenomas and invasive adenocarcinomas is the consequence of a succession of genetic and epigenetic changes in the normal colonic epithelium.  ...  It also provides conceptual knowledge of nanotechnology-based diagnostic techniques and therapeutic approaches for malignant colorectal cancer.  ...  Colorectal carcinogenesis has been linked to three distinct molecular pathways, including chromosomal instability (CIN), microsatellite instability (MSI), and chromosomal mismatch repair (CIMP) (Figure  ... 
doi:10.3390/nano12010169 pmid:35010119 pmcid:PMC8746463 fatcat:v2jclhpxsvc65jup7rxyqzpssu

Parallel Sessions (Proffered Papers)

2010 Analytical Cellular Pathology  
Neuroblastoma is the most common extracranial solid tumor in childhood and shows notable heterogeneity with regard to both histology and clinical behavior.  ...  DNA content in colon adenocarcinomas measured by image cytometry is an independent predictor of prognosis in our patients operated for colon adenocarcinoma.  ...  No nonsense mutations were found. 33% of non CD-related SBA showed microsatellite instability, whereas 67% of CD-related SBA were microsatellite instable.  ... 
doi:10.1155/2010/531968 fatcat:xfboajtlebgtvcnqkej5olrncm
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