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3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications

Christian Zuppinger
<span title="2019-06-26">2019</span> <i title="Frontiers Media SA"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dyhiozglrzgszfrxloytlygd7u" style="color: black;">Frontiers in Cardiovascular Medicine</a> </i> &nbsp;
Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs.  ...  Nevertheless, 3D cell culture is not as widespread or standardized as traditional cell culture methods using monolayers of cells on flat surfaces.  ...  Hospital Bern) for support and discussions.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3389/fcvm.2019.00087">doi:10.3389/fcvm.2019.00087</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/31294032">pmid:31294032</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6606697/">pmcid:PMC6606697</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/3a57ix7pyza7hhbowouy3enzpu">fatcat:3a57ix7pyza7hhbowouy3enzpu</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200215064717/https://fjfsdata01prod.blob.core.windows.net/articles/files/444234/pubmed-zip/.versions/1/.package-entries/fcvm-06-00087/fcvm-06-00087.pdf?sv=2015-12-11&amp;sr=b&amp;sig=kzNkaknrjJeSgj4C0NRnH6T029u1Czf1EdZvxvDBHZc%3D&amp;se=2020-02-15T06%3A47%3A39Z&amp;sp=r&amp;rscd=attachment%3B%20filename%2A%3DUTF-8%27%27fcvm-06-00087.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/52/9a/529a714861c2f288178dc8ad318596bd358db3fb.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3389/fcvm.2019.00087"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> frontiersin.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606697" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

A Microfluidic Multisize Spheroid Array for Multiparametric Screening of Anticancer Drugs and Blood–Brain Barrier Transport Properties

Christoph Eilenberger, Mario Rothbauer, Florian Selinger, Anna Gerhartl, Christian Jordan, Michael Harasek, Barbara Schädl, Johannes Grillari, Julian Weghuber, Winfried Neuhaus, Seta Küpcü, Peter Ertl
<span title="2021-03-24">2021</span> <i title="Wiley"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ifj4v2fyuvegzgqkhtt4dthbna" style="color: black;">Advanced Science</a> </i> &nbsp;
The reproducible on-chip generation of 360 spheroids of five dimensions on a well-plate format using an integrated microlens technology is demonstrated.  ...  A further application includes optimizing cell seeding ratios and size-dependent compound uptake studies in a perfused BBB model.  ...  The authors acknowledge the TU Wien University Library for financial support through its Open Access Funding Program. Conflict of Interest The authors declare no conflict of interest.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.202004856">doi:10.1002/advs.202004856</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/34105271">pmid:34105271</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC8188192/">pmcid:PMC8188192</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/y6tadgqrjngafp5b3d3pwjgvau">fatcat:y6tadgqrjngafp5b3d3pwjgvau</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210718003313/https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/advs.202004856" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/d5/a9/d5a9df826674254ad4ecbef400f710ca523723f6.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.202004856"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> wiley.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188192" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Fusing spheroids to aligned μ-tissues in a Heart-on-Chip featuring oxygen sensing and electrical pacing capabilities [article]

Oliver Schneider, Alessia Mouzzi, Stefanie Fuchs, Alina Grobel, Henrike Schulze, Torsten Mayr, Peter Loskill
<span title="2022-03-01">2022</span> <i title="Cold Spring Harbor Laboratory"> bioRxiv </i> &nbsp; <span class="release-stage" >pre-print</span>
We report on a novel integration strategy of optical O2 sensor spots into resin-based microfluidic systems, enabling in situ determination of O2 partial pressures.  ...  The developed system thus opens the door for advanced OOCs integrating biophysical stimulation as well as probing capabilities and serves as blueprint for the facile and robust generation of high density  ...  Time-lapse of tissue formation Following spheroids injection, pipet tips in tissue inlet & outlet were exchanged to filter tips containing fresh prewarmed media (100 µl per tip) and fixated on the motorized  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2022.02.26.482011">doi:10.1101/2022.02.26.482011</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/sfk55qfudfhyfdkbx5y6rmieuq">fatcat:sfk55qfudfhyfdkbx5y6rmieuq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20220424081030/https://www.biorxiv.org/content/biorxiv/early/2022/03/01/2022.02.26.482011.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/97/1d/971dad6533549d6df1538a460642923b7e7a9146.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2022.02.26.482011"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> biorxiv.org </button> </a>

Reproducible Construction of Surface Tension-Mediated Honeycomb Concave Microwell Arrays for Engineering of 3D Microtissues with Minimal Cell Loss

GeonHui Lee, JaeSeo Lee, HyunJik Oh, SangHoon Lee, Arum Han
<span title="2016-08-11">2016</span> <i title="Public Library of Science (PLoS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/s3gm7274mfe6fcs7e3jterqlri" style="color: black;">PLoS ONE</a> </i> &nbsp;
To evaluate the performance of the honeycomb microwell array, rat hepatocytes were seeded, and spheroids were successfully formed with uniform shape and size.  ...  More specifically, in order to control the curvature and shape of the concavity in a precise and reproducible manner, a custom-made jig system was designed and fabricated.  ...  Spheroid size distribution To determine the effect of each culture condition on the size of the spheroids, we analyzed the diameters of hepatocyte spheroids cultured for 7 days using Image J software  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1371/journal.pone.0161026">doi:10.1371/journal.pone.0161026</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/27513567">pmid:27513567</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC4981302/">pmcid:PMC4981302</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/qu7do5rcpjetnmrxxizcjvlswy">fatcat:qu7do5rcpjetnmrxxizcjvlswy</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20171012001341/http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0161026&amp;type=printable" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/0f/ce/0fce224eeb7629aeb6688bf04a60e3c006990ebc.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1371/journal.pone.0161026"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> plos.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981302" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing

Farah Tasnim, Nisha Hari Singh, Elijah Keng Foo Tan, Jiangwa Xing, Huan Li, Sebastien Hissette, Sravanthy Manesh, Justina Fulwood, Kapish Gupta, Chan Way Ng, Shuoyu Xu, Jeffrey Hill (+1 others)
<span title="2020-03-16">2020</span> <i title="Springer Science and Business Media LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tnqhc2x2aneavcd3gx5h7mswhm" style="color: black;">Scientific Reports</a> </i> &nbsp;
However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing  ...  and formation.  ...  Acknowledgements The authors thank Nikhil Mittal for valuable help and discussions. This work is supported in by grant H18/01/ a0/d14 as part of the A*STAR Innovations in  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41598-020-61699-4">doi:10.1038/s41598-020-61699-4</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/32179810">pmid:32179810</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/ve3oemecizazjopv7syp6w766m">fatcat:ve3oemecizazjopv7syp6w766m</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200317130019/https://www.nature.com/articles/s41598-020-61699-4.pdf?error=cookies_not_supported&amp;code=a5c20df3-4838-41d5-a58d-2724d8c4e631" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/b5/53/b5530f3e90396830b8ae8d508dba983ee9a00e71.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41598-020-61699-4"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a>

Nanotoxicity in Cancer Research: Technical Protocols and Considerations for the Use of 3D Tumour Spheroids [chapter]

Dania Movia, Adriele Prina-Mello
<span title="2018-03-21">2018</span> <i title="InTech"> Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications </i> &nbsp;
To address such need, protocols for the formation and characterisation of scaffold-free 3D tumour spheroids of human adenocarcinoma cells were developed and optimised in this study for their application  ...  One of the major reasons for drugs failure, accounting for around 60% of failed clinical trials, is the lack of efficacy in humans [8-10], albeit the therapeutic effect was robustly demonstrated earlier  ...  Acknowledgements The authors would like to thank Biocroi Ltd for the materials supplied free-of-charge, and Kieran Crosbie Staunton for Western blotting.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.5772/intechopen.69447">doi:10.5772/intechopen.69447</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/boxtsxea4ndnzjjc5aw4wjw7fq">fatcat:boxtsxea4ndnzjjc5aw4wjw7fq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190501153203/https://cdn.intechopen.com/pdfs/55788.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/7d/ab/7dab556a31725edd63977b3087f5435f58d6daa5.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.5772/intechopen.69447"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications

Ben Wang, Kai Fung Chan, Fengtong Ji, Qianqian Wang, Philip Wai Yan Chiu, Zhiguang Guo, Li Zhang
<span title="2019-03-14">2019</span> <i title="Wiley"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ifj4v2fyuvegzgqkhtt4dthbna" style="color: black;">Advanced Science</a> </i> &nbsp;
Delivery of the cell spheroid to a hydrophilic surface results in the in situ respreading of cells and gradual formation of typical 2D cell morphology, which offers the possibility for such spheroid-based  ...  The formation, coalescence, and splitting of LMs can be realized on-demand with a designed channel box.  ...  The system offers researchers a new choice for producing SCSs with a large size, high cell viability, and strong internal cell-to-cell contact, which is a useful in vitro model to study the formation and  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.201802033">doi:10.1002/advs.201802033</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/31131188">pmid:31131188</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6523389/">pmcid:PMC6523389</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/qso4f6icxncjfmuysz3cbmdzpi">fatcat:qso4f6icxncjfmuysz3cbmdzpi</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20191017182258/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6523389&amp;blobtype=pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/d8/1e/d81ec7fea6210079b69c5abdd9f61fb6da4e0644.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1002/advs.201802033"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> wiley.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523389" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels

Andrew C. Daly, Matthew D. Davidson, Jason A. Burdick
<span title="2021-02-02">2021</span> <i title="Springer Science and Business Media LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/a4wan6l5o5dfzn767kyz7jqevi" style="color: black;">Nature Communications</a> </i> &nbsp;
AbstractCellular models are needed to study human development and disease in vitro, and to screen drugs for toxicity and efficacy.  ...  This method is useful for a range of biomedical applications, including the development of precision models to mimic diseases and the screening of drugs, particularly where high cell densities and heterogeneity  ...  well as additional funding through the National Science Foundation supported Center for Engineering MechanoBiology STC (CMMI: and the National Institutes of Health (R01HL137365, R01HL135090).  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41467-021-21029-2">doi:10.1038/s41467-021-21029-2</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/33531489">pmid:33531489</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/ggywhqxmfjbuvpo7ibutszhh6m">fatcat:ggywhqxmfjbuvpo7ibutszhh6m</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210429072838/https://www.nature.com/articles/s41467-021-21029-2.pdf?error=cookies_not_supported&amp;code=fbcd3902-25f7-4ce4-aa1e-190dee232b95" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/a9/99/a999d62194b9afa6c1914f3cf337a1f27bc4fd8d.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41467-021-21029-2"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> nature.com </button> </a>

Microscale Mass Spectrometry Analysis of Extracellular Metabolites in Live Multicellular Tumor Spheroids

Mei Sun, Xiang Tian, Zhibo Yang
<span title="2017-08-16">2017</span> <i title="American Chemical Society (ACS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/nx5fizmox5he5k23oobehznqna" style="color: black;">Analytical Chemistry</a> </i> &nbsp;
However, the lack of appropriate techniques leads to limited studies of extracellular metabolite.  ...  This study provides a novel way to detect the extracellular compounds inside live spheroids, and the successful development of our technique can benefit the research in multiple areas, including the microenvironment  ...  Anthony Burgett and Naga Rama Kothapalli (the University of Oklahoma) for providing the HCT-116 cells.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.7b01746">doi:10.1021/acs.analchem.7b01746</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/28753268">pmid:28753268</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5912160/">pmcid:PMC5912160</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/wianmbczgnhnnpyytnts3vsg3a">fatcat:wianmbczgnhnnpyytnts3vsg3a</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200208075713/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5912160&amp;blobtype=pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/93/8b/938beea54d4ee4a003a46b91fc68fb3842eaea44.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.7b01746"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> acs.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912160" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

Brigitte Altmann, Christoph Grün, Cordula Nies, Eric Gottwald
<span title="2020-12-23">2020</span> <i title="MDPI AG"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/vt2hc3xcijfofb7cwnxv4hhszi" style="color: black;">Processes</a> </i> &nbsp;
Because at least one of these parameters can be assigned to the 3D cell culture techniques discussed in the present review, we structured the studies according to the complexity levels applied in the MBR  ...  As our literature research revealed a variety of 3D cell culture configurations in the examined bioreactor systems, we defined in review part one "complexity levels" by means of the corresponding 3D cell  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/pr9010021">doi:10.3390/pr9010021</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/6xr4uyxvsrcdpfj6j4ttqooacq">fatcat:6xr4uyxvsrcdpfj6j4ttqooacq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20201228032026/https://res.mdpi.com/d_attachment/processes/processes-09-00021/article_deploy/processes-09-00021-v2.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/f6/e3/f6e32e4d2012cdbb31922b4f645e8845b82654e3.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.3390/pr9010021"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> mdpi.com </button> </a>

Tumor invasion as non-equilibrium phase separation [article]

Wenying Kang, Jacopo Ferruzzi, Catalina-Paula Spatarelu, Yu Long Han, Yasha Sharma, Stephan Koehler, James P. Butler, Darren Roblyer, Muhammad H. Zaman, Ming Guo, Zi Chen, Adrian F. Pegoraro (+1 others)
<span title="2020-04-29">2020</span> <i title="Cold Spring Harbor Laboratory"> bioRxiv </i> &nbsp; <span class="release-stage" >pre-print</span>
Using a multicellular spheroid embedded within an engineered three-dimensional matrix, we show here the potential for coexistence of solid-like, fluid-like, and gas-like phases of the cellular collective  ...  In the case of MDA-MB-231, for example, we find that when ECM density is 2 mg/ml or smaller single cells and cell clusters scatter from the spheroid periphery in the form of discrete gas-like particles  ...  P06G-0-10-F) by pipetting 5 μl drops on each of the coverslips.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.04.28.066845">doi:10.1101/2020.04.28.066845</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/5ebtla726rcndm7faf43iazrka">fatcat:5ebtla726rcndm7faf43iazrka</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200711132515/https://www.biorxiv.org/content/biorxiv/early/2020/04/29/2020.04.28.066845.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/df/e5/dfe56650b652a59967672ed98405a3aeca039e25.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/2020.04.28.066845"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> biorxiv.org </button> </a>

Studying 3D cell cultures in a microfluidic droplet array under multiple time-resolved conditions [article]

Raphael Tomasi, Sebastien Sart, Tiphaine Champetier, Charles Baroud
<span title="2018-09-04">2018</span> <i title="Cold Spring Harbor Laboratory"> bioRxiv </i> &nbsp; <span class="release-stage" >pre-print</span>
However the methods for spheroid manipulation are still labor intensive, which severely limits the complexity of operations that can be performed on statistically relevant numbers of individual spheroids  ...  In this context, spheroids are an appealing 3D cell culture format to complement standard techniques, by combining a high level of biological relevance with simple production protocols.  ...  Beyond these illustrative examples, the ability to manipulate individual spheroids allows us to study the toxicity of a drug (acetaminophen) on hepatocyte 3D cultures.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/407759">doi:10.1101/407759</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/yniboouljvac5guqijjvrt7o3e">fatcat:yniboouljvac5guqijjvrt7o3e</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190504002055/https://www.biorxiv.org/content/biorxiv/early/2018/09/04/407759.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/38/ab/38abf8419214cc48d3cacc6ab0c8a70159e0fd63.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1101/407759"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> biorxiv.org </button> </a>

3D modeling of cancer stem cell niche

Jun He, Li Xiong, Qinglong Li, Liangwu Lin, Xiongying Miao, Shichao Yan, Zhangyong Hong, Leping Yang, Yu Wen, Xiyun Deng
<span title="2017-08-03">2017</span> <i title="Impact Journals, LLC"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/yubgl6cdcrekxpjzhshpw23l3i" style="color: black;">OncoTarget</a> </i> &nbsp;
These in vitro culture systems recapitulate the spatial dimension, cellular heterogeneity, and the molecular networks of the tumor microenvironment and show great promise in elucidating the pathophysiology  ...  of cancer stem cells and designing more clinically relavant treatment modalites.  ...  On the other hand, in-chip spheroid formation is also implemented for high-throughput screening of drugs.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.18632/oncotarget.19847">doi:10.18632/oncotarget.19847</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/29416698">pmid:29416698</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC5787442/">pmcid:PMC5787442</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/vhhf5e5wvvcofeivvtdqphebae">fatcat:vhhf5e5wvvcofeivvtdqphebae</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190224001923/http://pdfs.semanticscholar.org/5955/865a53730495928dde6da9a10ab5816e0c1c.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/59/55/5955865a53730495928dde6da9a10ab5816e0c1c.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.18632/oncotarget.19847"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787442" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Cell and Nucleus Shape as an Indicator of Tissue Fluidity in Carcinoma

Steffen Grosser, Jürgen Lippoldt, Linda Oswald, Matthias Merkel, Daniel M. Sussman, Frédéric Renner, Pablo Gottheil, Erik W. Morawetz, Thomas Fuhs, Xiaofan Xie, Steve Pawlizak, Anatol W. Fritsch (+6 others)
<span title="2021-02-17">2021</span> <i title="American Physical Society (APS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/34iggvr2oraezibenzxlccincy" style="color: black;">Physical Review X</a> </i> &nbsp;
Cancer cell motility requires and corresponds to a fluidization of the tumor tissue on the bulk level.  ...  In patient samples of mamma and cervix carcinoma, we find areas where cells can move or are immobile. We compare 3D cell spheroids composed of cells from a cancerous and a noncancerous cell line.  ...  Previous studies in 3D systems focus on cell escape from multicellular tumor spheroids into the extracellular matrix (ECM) [30, 31] .  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1103/physrevx.11.011033">doi:10.1103/physrevx.11.011033</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/uw2ibrls3zefjmxhrtidtiosum">fatcat:uw2ibrls3zefjmxhrtidtiosum</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210718162329/https://hal.archives-ouvertes.fr/hal-03158879/file/Grosser%20et%20al.%20-%202021%20-%20Physical%20Review%20X%20-%20Cell%20and%20Nucleus%20Shape%20as%20an%20Indicator%20of%20Tissue%20Fluidity%20in%20Carcinoma.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/ec/d7/ecd7b45d6801cd0b122ff654daec847a4004be22.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1103/physrevx.11.011033"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> aps.org </button> </a>

Generation of lung organoids from human pluripotent stem cells in vitro

Alyssa J. Miller, Briana R. Dye, Daysha Ferrer-Torres, David R. Hill, Arend W. Overeem, Lonnie D. Shea, Jason R. Spence
<span title="2019-01-21">2019</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/z2jd6b73erhgxdr3pwdyqxtsta" style="color: black;">Nature Protocols</a> </i> &nbsp;
Here we describe a protocol that recapitulates several of these milestones in order to differentiate human pluripotent stem cells (hPSCs) into ventral-anterior foregut spheroids and further into two distinct  ...  The bud tip progenitor organoids are thus ideal for exploring epithelial fate decisions, while the human lung organoids can be used to model epithelial-mesenchymal cross-talk during human lung development  ...  The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41596-018-0104-8">doi:10.1038/s41596-018-0104-8</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/30664680">pmid:30664680</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6531049/">pmcid:PMC6531049</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/4xctcx7srzdndl3i6cxpcfnup4">fatcat:4xctcx7srzdndl3i6cxpcfnup4</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200321143322/https://www.rug.nl/research/portal/files/91994105/s41596_018_0104_8.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/f7/36/f736f4c7b31798df952e68e3401ee15c58eacf81.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/s41596-018-0104-8"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> nature.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531049" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>
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