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Spontaneous Formation of Extensive Vessel-Like Structures in Murine Engineered Heart Tissue

Andrea Stoehr, Marc N. Hirt, Arne Hansen, Moritz Seiffert, Lenard Conradi, June Uebeler, Florian P. Limbourg, Thomas Eschenhagen
2016 Tissue Engineering. Part A  
The EC iLacZ EHT model could help simultaneously study biological effects of compounds on cardiomyocyte function and tissue vascularization.  ...  Coherent contractility and the presence of an extensive vessel-like network were both dependent on the presence of animal sera in the culture medium.  ...  This study was supported by funds from the Deutsche Forschungsgemeinschaft (DFG Es 88/9-2, Es 88/12-1, and GEROK SE 2118/1) and the European Union (FP7 Angioscaff, FP7 Biodesign, and FP7 Big-Heart).  ... 
doi:10.1089/ten.tea.2015.0242 pmid:26763667 pmcid:PMC4799701 fatcat:jfyssdr6bjbhpjavvx2skdej7i

Hydrodynamics and bioprocess considerations in designing bioreactors for cardiac tissue engineering

Ailing Teo, Athanasios Mantalaris, Mayasari Lim
2012 journal of Regenerative Medicine and Tissue Engineering  
This paper provides a critical review on the hydrodynamic considerations with respect to design aspects of the bioreactor and selection of substrate material for 3D cardiac tissue engineering.  ...  Control of bulk and microscopic transport efficiency, which is determined by the choice of the bioreactor design and substrate material, poses direct influence on cell viability, proliferation and differentiation  ...  The narrowing of blood vessels in coronary heart disease patients limits the supply of blood to the heart and leads to myocardial ischemia.  ... 
doi:10.7243/2050-1218-1-4 fatcat:fj3d75htzrdenok3pchl4emriu

Strategies for ensuring that regenerative cardiomyocytes function properly and in cooperation with the host myocardium

Fumiyuki Hattori, Keiichi Fukuda
2010 Experimental and Molecular Medicine  
Nowadays, heart failure is one of the major causes of death. In spite of this, the therapeutic efficacies of medications are generally unsatisfactory.  ...  In this review, we summarize the various strategies for using regenerative cardiomyocytes including our recent progressions: non-genetic approaches for the purification of cardiomyocytes and efficient  ...  Histological analysis of the transplanted cells revealed that the cardiac blood vessels contained cardiomyocytes.  ... 
doi:10.3858/emm.2010.42.3.022 pmid:20164677 pmcid:PMC2845000 fatcat:kijdptti2zb3zo2eitzmmogyxq

Review of Single-Cell RNA Sequencing in the Heart

Shintaro Yamada, Seitaro Nomura
2020 International Journal of Molecular Sciences  
Cardiovascular disease is one of the major causes of death worldwide and its precise pathology remains unclear. scRNA-seq has provided many novel insights into both healthy and pathological hearts.  ...  In this review, we summarize the various scRNA-seq platforms and describe the molecular mechanisms of cardiovascular development and disease revealed by scRNA-seq analysis.  ...  Consequently, healthy/pathological differentiation and developmental processes have been well studied in embryonic and neonatal murine hearts. scRNA-seq of CMs from murine embryonic or neonatal hearts  ... 
doi:10.3390/ijms21218345 pmid:33172208 pmcid:PMC7664385 fatcat:7asvhisw3rgwhfjb4of57cw6vq

Undernutritionin UteroAugments Systolic Blood Pressure and Cardiac Remodeling in Adult Mouse Offspring: Possible Involvement of Local Cardiac Angiotensin System in Developmental Origins of Cardiovascular Disease

Makoto Kawamura, Hiroaki Itoh, Shigeo Yura, Haruta Mogami, Shin-Ichi Suga, Hisashi Makino, Yoshihiro Miyamoto, Yasunao Yoshimasa, Norimasa Sagawa, Shingo Fujii
2007 Endocrinology  
< 0.01), perivas-cular fibrosis of the coronary artery (16 wk, P < 0.05) and cardiac cardiomegaly (16 wk, P < 0.01), and cardiomyocyte enlargement, concomitant with a significant augmentation of angiotensinogen  ...  in later life, in concert with the effects of increase in blood pressure.  ...  Quantitative RT-PCR analysis Total RNA was extracted from whole hearts of fetal mice at 18.5 dpc and from left ventricles of the mice at 3, 8, and 16 wk, as well as from kidneys at 16 wk.  ... 
doi:10.1210/en.2006-0706 pmid:17138658 fatcat:2mvpel6h2vb5rbgg7svnpqgxzu

Soluble epoxide hydrolase plays an essential role in angiotensin II-induced cardiac hypertrophy

D. Ai, W. Pang, N. Li, M. Xu, P. D. Jones, J. Yang, Y. Zhang, N. Chiamvimonvat, J. Y.-J. Shyy, B. D. Hammock, Y. Zhu
2009 Proceedings of the National Academy of Sciences of the United States of America  
These results were supported by studies in neonatal cardiomyocytes from sEH ؊/؊ mice.  ...  In vitro, Ang II upregulated sEH and hypertrophy markers in neonatal cardiomyocytes isolated from rat and mouse.  ...  This study was supported in part by Grants 30570713 and 30630032 from the National Natural Science  ... 
doi:10.1073/pnas.0811022106 pmid:19126686 pmcid:PMC2626743 fatcat:e7c765hgzfgh3n77haf5mmvgbm

Extracellular Matrix-Based Biomaterials for Cardiovascular Tissue Engineering

Astha Khanna, Maedeh Zamani, Ngan F. Huang
2021 Journal of Cardiovascular Development and Disease  
techniques, the regulation of ECMs on vascular differentiation and function, and the translation of ECM-based scaffolds for vascular graft applications.  ...  The design of three-dimensional (3D) scaffolds with appropriate biochemical and mechanical characteristics is critical for engineering tissue-engineered replacements.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/jcdd8110137 pmid:34821690 pmcid:PMC8622600 fatcat:ztqmxsw4lbhy7f2pbwyanqx2qe

Reduced Cardiac L-Type Ca2+ Current in Cav 2-/- Embryos Impairs Cardiac Development and Contraction With Secondary Defects in Vascular Maturation

P. Weissgerber
2006 Circulation Research  
This led to a functionally compromised heart, causing defective remodeling of intraand extraembryonic blood vessels and embryonic death following E10.5.  ...  Cardiac myocyte contraction depends on transmembrane L-type Ca 2ϩ currents and the ensuing release of Ca 2ϩ from the sarcoplasmic reticulum.  ...  Sources of Funding Disclosures None.  ... 
doi:10.1161/01.res.0000243978.15182.c1 pmid:16946137 fatcat:cspxkpqxl5aidpdcgsyvfokkua

Ablation of periostin inhibits post-infarction myocardial regeneration in neonatal mice mediated by the phosphatidylinositol 3 kinase/glycogen synthase kinase 3β/cyclin D1 signalling pathway

Zhenhuan Chen, Jiahe Xie, Huixin Hao, Hairuo Lin, Long Wang, Yingxue Zhang, Lin Chen, Shiping Cao, Xiaobo Huang, Wangjun Liao, Jianping Bin, Yulin Liao
2017 Cardiovascular Research  
The extent of new blood vessels was evaluated based on the density of endothelial cells using the ratio of positively stained area to gross area.  ...  Becker et al. 39 also demonstrated in vitro that ANP induced proliferation of neonatal murine cardiomyocytes.  ... 
doi:10.1093/cvr/cvx001 pmid:28453729 pmcid:PMC5412017 fatcat:nqxiwhnhwzfhdorlxkxjpnromi

Effect of Mechanical Loading on Three-Dimensional Cultures of Embryonic Stem Cell-Derived Cardiomyocytes

Valerie F. Shimko, William C. Claycomb
2008 Tissue engineering  
This study describes a 3-dimensional model to examine the direct effect of mechanical loading on the differentiation of ESC-derived cardiomyocytes embedded in a defined extracellular matrix scaffold.  ...  Mechanical loading significantly affected gene expression, and these changes were dependent on the frequency of stretch.  ...  May Lam for developing the ESC culture technique and selecting the ESCs, Gary Seibel (University of Maryland) for component machining, and the LSUHSC histopathology laboratory for histology.  ... 
doi:10.1089/ten.2007.0092 fatcat:5kp2azhdsjh3beel2g3r2qmyty

Loss of miR-132/212 Has No Long-Term Beneficial Effect on Cardiac Function After Permanent Coronary Occlusion in Mice

Zhiyong Lei, Juntao Fang, Janine C. Deddens, Corina H. G. Metz, Esther C. M. van Eeuwijk, Hamid el Azzouzi, Pieter A. Doevendans, Joost P. G. Sluijter
2020 Frontiers in Physiology  
In this study, we explored the role of miR-132/212 during ischemia in a murine MI model.  ...  In line with these in vivo observation, overexpression of miR-132 or miR-212 in neonatal rat cardiomyocyte suppress hypoxia induced cardiomyocyte cell death.  ...  Taro Fukao (Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany) for miR132/212 knockout mice; Maike Brans (University Medical Center Utrecht, Netherlands) for her excellent technical  ... 
doi:10.3389/fphys.2020.00590 pmid:32612537 pmcid:PMC7309700 fatcat:rnrszqhtzbg2rlunbbibnk3zxu

Effect of Mechanical Loading on Three-Dimensional Cultures of Embryonic Stem Cell-Derived Cardiomyocytes

Valerie F. Shimko, William C. Claycomb
2008 Tissue Engineering. Part A  
This study describes a 3-dimensional model to examine the direct effect of mechanical loading on the differentiation of ESC-derived cardiomyocytes embedded in a defined extracellular matrix scaffold.  ...  Mechanical loading significantly affected gene expression, and these changes were dependent on the frequency of stretch.  ...  May Lam for developing the ESC culture technique and selecting the ESCs, Gary Seibel (University of Maryland) for component machining, and the LSUHSC histopathology laboratory for histology.  ... 
doi:10.1089/ten.a.2007.0092 pmid:18333804 pmcid:PMC2562769 fatcat:dq6rknsi6rf2nbit2yi2fcl3ii

The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems

Sebastian Pieperhoff, Steffen Rickelt, Hans Heid, William C. Claycomb, Ralf Zimbelmann, Caecilia Kuhn, Stefanie Winter-Simanowski, Christian Kuhn, Norbert Frey, Werner W. Franke
2012 Journal of Cellular and Molecular Medicine  
cells of the mammalian blood and lymph vascular systems, including the desmoplakin-containing complexus adhaerentes of the virgultar cells of lymph node sinus.  ...  the composite junctions in the intercalated disks connecting the cardiomyocytes of mammalian hearts.  ...  of neonatal rat cardiomyocytes, or in HL-1 cardiomyocyte cultures ( Fig. 2A-C) .  ... 
doi:10.1111/j.1582-4934.2011.01463.x pmid:21992629 pmcid:PMC3822684 fatcat:ktntxyaasvebhnqbdlkemwsdyy

GATA4 regulatesFgf16to promote heart repair after injury

Wei Yu, Xiuzhen Huang, Xueying Tian, Hui Zhang, Lingjuan He, Yue Wang, Yu Nie, Shengshou Hu, Zhiqiang Lin, Bin Zhou, William Pu, Kathy O. Lui (+1 others)
2016 Development  
Using the neonatal mouse heart cryoinjury and apical resection models with an inducible loss of GATA4 specifically in cardiomyocytes, we found severely depressed ventricular function in the Gata4-ablated  ...  Importantly, replication of cardiomyocytes has been found to be the key mechanism responsible for neonatal cardiac regeneration.  ...  Zeng for the Rosa26-RFP mouse line and members of the Zhou laboratory for offering comments in preparation of this manuscript.  ... 
doi:10.1242/dev.130971 pmid:26893347 fatcat:xlp27flndbbuvdjowcg7fy6o6u

Cardiomyocyte-Restricted Overexpression of Endothelial Nitric Oxide Synthase (NOS3) Attenuates -Adrenergic Stimulation and Reinforces Vagal Inhibition of Cardiac Contraction

P. B. Massion
2004 Circulation  
Methods and Results-We studied the influence of NOS3 in relation to its subcellular localization on the function of cardiomyocytes isolated from transgenic mice overexpressing NOS3 under the ␣-myosin heavy  ...  Conclusions-Moderate overexpression of NOS3, targeted to caveolae in murine cardiomyocytes, potentiates the postsynaptic muscarinic response and attenuates the effect of high concentrations of catecholamines  ...  Immunoblotted eNOS proteins are compared in extracts of NOS3-TG and wild-type (WT) neonatal cardiomyocytes after 8 days in culture.  ... 
doi:10.1161/01.cir.0000145608.80855.bc pmid:15492314 fatcat:b53gstfidnb4vhfomv4g2do5j4
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