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Comparative computational analysis of ADP Glucose Pyrophosphorylase in plants

Saroj Rani, Pradeep Sharma, Anil Sharma, Ravish Chatrath
2013 Bioinformation  
ADP-glucose pyrophosphorylase (AGPase), a key enzyme involved in higher plant starch biosynthesis, is composed of pairs of large (LS) and small subunits (SS).  ...  In this study, we compiled detailed comparative information about ADP glucose pyrophosphorylase in selected plants by analyzing their structural features e.g. amino acid content, physico-chemical properties  ...  Technology, Ministry of Communications & Information Technology, Government of India, New Delhi to RC and Indian Council of Agricultural research of New Delhi, India to PS.  ... 
doi:10.6026/97320630009572 pmid:23888098 pmcid:PMC3717185 fatcat:mkmk6nrprvb3lfigidxieqjwe4

Both Subunits of ADP-Glucose Pyrophosphorylase Are Regulatory

J. M. Cross
2004 Plant Physiology  
The allosteric enzyme ADP-Glc pyrophosphorylase (AGPase) catalyzes the synthesis of ADP-Glc, a rate-limiting step in starch synthesis.  ...  We exploited an Escherichia coli expression system and mosaic AGPases composed of potato (Solanum tuberosum) tuber and maize (Zea mays) endosperm subunit fragments to pursue this objective.  ...  Regions in ADP-Glucose Pyrophosphorylase Plant Physiol.  ... 
doi:10.1104/pp.103.036699 pmid:15122037 pmcid:PMC429341 fatcat:3w6bdqkqvratnjokmb225lvyjq

ADP-Glucose Pyrophosphorylase, a Regulatory Enzyme for Bacterial Glycogen Synthesis

M. A. Ballicora, A. A. Iglesias, J. Preiss
2003 Microbiology and Molecular Biology Reviews  
Prediction of secondary structure of ADP-glucose pyrophosphorylases. The secondary structure of various ADP-Glc PPases from bacteria as well as plants was predicted with the PHD program (81) .  ...  VOL. 67, 2003 REGULATION OF ADP-GLUCOSE PYROPHOSPHORYLASE 215 .  ... 
doi:10.1128/mmbr.67.2.213-225.2003 pmid:12794190 pmcid:PMC156471 fatcat:wf4iqktnu5g3hefz6hbgllhnhu

Molecular Architecture of the Glucose 1-Phosphate Site in ADP-glucose Pyrophosphorylases

Clarisa Maria Bejar, Xiangshu Jin, Miguel Angel Ballicora, Jack Preiss
2006 Journal of Biological Chemistry  
The initial and key regulatory step is the formation of the glucosyl donor molecule ADP-Glc from ATP and Glc-1-P via a reaction catalyzed by ADP-glucose pyrophosphorylase (PPase 4 ; glucose-1-phosphate  ...  ADP-Glc pyrophosphorylase (PPase), a key regulatory enzyme in the biosynthetic pathway of starch and bacterial glycogen, catalyzes the synthesis of ADP-Glc from Glc-1-P and ATP.  ...  Iglesias for stimulating discussions and critical review of the manuscript.  ... 
doi:10.1074/jbc.m607088200 pmid:17079236 fatcat:54li3my5bneppc4gf7qh6vg6f4

ATP binding site in the plant ADP-glucose pyrophosphorylase large subunit

Seon-Kap Hwang, Shigeki Hamada, Thomas W. Okita
2006 FEBS Letters  
The ATP binding region in the catalytically inactive large subunit (LS) of the potato tuber ADP-glucose pyrophosphorylase was identified and investigated.  ...  The catalytic rates of the LS mutant enzymes correlated most strongly with changes in the affinity toward ATP, a relationship substantiated by photoaffinity labeling studies with azido-ATP analog.  ...  Abbreviations: AGPase, ADP-glucose pyrophosphorylase; DTT, dithiothreitol; Glc-1-P, glucose-1-phosphate; L or LS, large subunit; PDB, Protein Data Bank; 3-PGA, 3-phosphoglycerate; S or SS, small subunit  ... 
doi:10.1016/j.febslet.2006.11.029 pmid:17137579 fatcat:zt2gdmg5qfhhvaoxnaaa5vtg6y

Characterization of an Autonomously Activated Plant ADP-Glucose Pyrophosphorylase

S. K. Boehlein, J. R. Shaw, J. D. Stewart, L. C. Hannah
2008 Plant Physiology  
ADP-glucose pyrophosphorylase (AGPase) catalyzes the rate-limiting step in starch biosynthesis in plants and changes in its catalytic and/or allosteric properties can lead to increased starch production  ...  the potato tuber enzyme, was expressed with the maize endosperm large subunit and was reported to have favorable kinetic and allosteric properties.  ...  Plant Physiol 146: 289-299 Autonomously Activated ADP-Glucose Pyrophosphorylase Plant Physiol.  ... 
doi:10.1104/pp.108.126862 pmid:18715954 pmcid:PMC2613723 fatcat:wnghhrr535h37baqfvt7u4mxn4

Insights of interaction between small and large subunits of ADP-glucose pyrophosphorylase from bread wheat (Triticum aestivum L.)

Mohd Danishuddin, Ravish Chatrath, Rajender Singh
2011 Bioinformation  
Lack of knowledge of three dimensional structures of small and large subunits of ADP-glucose pyrophosphorylase (AGPase) in wheat has hindered efforts to understand the binding specifities of substrate  ...  Thus, to understand the structure activity relationship, 3D structures were built by homology modelling based on crystal structure of potato tuber ADP-glucose pyrophosphorylase.  ...  Technology, Government of India, New Delhi is gratefully acknowledged.  ... 
doi:10.6026/97320630006144 pmid:21572880 pmcid:PMC3092947 fatcat:7j4apbr7rzep5pryqgxhu45iai

Probing Allosteric Binding Sites of the Maize Endosperm ADP-Glucose Pyrophosphorylase

S. K. Boehlein, J. R. Shaw, L. C. Hannah, J. D. Stewart
2009 Plant Physiology  
Although the structure of the heterotetrameric maize endosperm AGPase remains unsolved, structures of a nonnative, low-activity form of the potato tuber (Solanum tuberosum) AGPase (small subunit homotetramer  ...  Maize (Zea mays) endosperm ADP-glucose pyrophosphorylase (AGPase) is a highly regulated enzyme that catalyzes the rate-limiting step in starch biosynthesis.  ...  ADP-Glucose Pyrophosphorylase Plant Physiol.  ... 
doi:10.1104/pp.109.146928 pmid:19889875 pmcid:PMC2799348 fatcat:3ytt2m4p75cpfcqyeiyegvirle

The ancestral activation promiscuity of ADP-glucose pyrophosphorylases from oxygenic photosynthetic organisms

Misty L Kuhn, Carlos M Figueroa, Alberto A Iglesias, Miguel A Ballicora
2013 BMC Evolutionary Biology  
ADP-glucose pyrophosphorylase (ADP-Glc PPase) catalyzes the first committed step in the synthesis of glycogen in bacteria and starch in algae and plants.  ...  O. tauri and potato tuber enzymes were heterotetramers comprising homologous small and large subunits.  ...  Data of sequences used for the phylogenetic tree.  ... 
doi:10.1186/1471-2148-13-51 pmid:23433303 pmcid:PMC3585822 fatcat:dwezuszeb5cnbcw7gbnrxsl3i4

Duplications and functional divergence of ADP-glucose pyrophosphorylase genes in plants

Nikolaos Georgelis, Edward L Braun, L Curtis Hannah
2008 BMC Evolutionary Biology  
ADP-glucose pyrophosphorylase (AGPase), which catalyses a rate limiting step in starch synthesis, is a heterotetramer comprised of two identical large and two identical small subunits in plants.  ...  The large subunit also had a larger number of gene duplications than did the small subunit.  ...  Acknowledgements We thank Rebecca Kimball and members of the Kimball, Braun and Hannah laboratories for many useful comments and discussions.  ... 
doi:10.1186/1471-2148-8-232 pmid:18700010 pmcid:PMC2529307 fatcat:kjfi4dtd2rdsxmyri3gdldr3le

Insights into Subunit Interactions in the Heterotetrameric Structure of Potato ADP-Glucose Pyrophosphorylase

Aytug Tuncel, Ibrahim Halil Kavakli, Ozlem Keskin
2008 Biophysical Journal  
ADP-glucose pyrophosphorylase, a key allosteric enzyme involved in higher plant starch biosynthesis, is composed of pairs of large (LS) and small subunits (SS).  ...  Recently the crystal structure of the SS homotetramer has been solved, but no crystal structure of the native heterotetrameric enzyme is currently available.  ...  Salamone for his critical reading of the manuscript.  ... 
doi:10.1529/biophysj.107.123042 pmid:18641076 pmcid:PMC2553129 fatcat:3oh272fjqzepre263t7mdfxi64

Ostreococcus tauriADP-glucose Pyrophosphorylase Reveals Alternative Paths for the Evolution of Subunit Roles

Misty L. Kuhn, Christine A. Falaschetti, Miguel A. Ballicora
2009 Journal of Biological Chemistry  
This enzyme catalyzes the reaction of ATP and Glc-1-P to form ADP-glucose (ADP-Glc) and PP i , and it is . 4 The abbreviations used are: ADP-Glc PPase, ADP-glucose pyrophosphorylase; OtaS, O. tauri S subunit  ...  The O. tauri enzyme was not redox-regulated, suggesting that redox regulation of ADP-glucose pyrophosphorylases appeared later in evolution.  ...  Olsen for revision of the manuscript.  ... 
doi:10.1074/jbc.m109.037614 pmid:19737928 pmcid:PMC2797180 fatcat:h7kh5bjw3vhwla7ggpj54hwrwm

Plant ADP-Glucose Pyrophosphorylase -Recent Advances and Biotechnological Perspectives (A Review)

Leszek A. Kleczkowski, Per Villand, Anders Lönneborg, Odd-Arne Olsen, Ernst Lüthi
1991 Zeitschrift für Naturforschung C - A Journal of Biosciences  
Recent advances in studies on plant ADP -glucose pyrophosphorylase (AGP), the key enzyme of starch biosynthesis, are presented.  ...  The importance of AGP in carbohydrate metabolism and several of its features, such as potent regulation by cellular effectors (3-phosphoglycerate and Pi), an unusual two subunit-types structure, tissue-specific  ...  Introduction ADP-glucose pyrophosphorylase (ATP: a-glucose-l-P adenylyl-transferase, EC 2.7.7.27) (AGP) is an im portant regulatory enzyme in the biosyn thesis of starch and glycogen in plants and bacte  ... 
doi:10.1515/znc-1991-7-817 fatcat:mun5ocgodfecniveltbtar53hi

Isolation and characterization of a higher plant ADP-glucose pyrophosphorylase small subunit homotetramer

Peter R. Salamone, Thomas W. Greene, Ibrahim H. Kavakli, Thomas W. Okita
2000 FEBS Letters  
ADP-glucose pyrophosphorylase (AGPase) is the allosterically regulated gateway for carbon entry into transient and storage starch in plants as well as glycogen in bacteria.  ...  AGPase is a tetrameric enzyme; in higher plants it consists of two regulatory large subunits (LS) and two catalytic small subunits (SS), while in cyanobacteria and prokaryotes the enzyme is homotetrameric  ...  The ¢rst step is the formation of adenosine 5P-diphosphate (ADP)glucose from glucose 1-P and ATP, a reaction catalyzed by ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27).  ... 
doi:10.1016/s0014-5793(00)01985-2 pmid:11018533 fatcat:njbuwq5wg5gj7ck6enwu6jbddu

Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)

C. Cheng, J. Hu, Y. Zhi, J.J. Su, X.K. Zhang, B.Q. Huang
2015 Genetics and Molecular Research  
The deduced translation product of the CeAGPS gene contained a well-conserved catalytic domain and regulatory elements typical of plant AGPS.  ...  Cloning and characterization of AGPS in yellow nutsedge genes.  ...  Conflicts of interest The authors declare no conflict of interest.  ... 
doi:10.4238/2015.december.23.18 pmid:26782478 fatcat:kmkytmnbarhxxh2lel3ldbgsxi
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