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A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand
1999
Journal of Molecular Recognition
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A. (1992). Designer dyes. Biomimetic ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol. 10, 442-448.
McLoughlin, S. B. and Lowe, C. R. (1988). ...
JOURNAL OF MOLECULAR RECOGNITION J. Mol. Recognit. 1999;12:67—75
A strategy for the generation of biomimetic ligands for affinity chromatography. ...
doi:10.1002/(sici)1099-1352(199901/02)12:1<67::aid-jmr443>3.0.co;2-4
pmid:10398398
fatcat:m7xvzxxyizhvlglt53bt6gzb2y
Purification of antibodies by affinity chromatography
2002
Journal of Biochemical and Biophysical Methods
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This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. ...
The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described. D ...
That is because Protein A has some important virtues for application as an affinity ligand. ...
doi:10.1016/s0165-022x(02)00017-9
pmid:12088882
fatcat:x7d6w3totzc2pnbopyrt2gpife
Recent Advances in Nanovaccines Using Biomimetic Immunomodulatory Materials
2019
Pharmaceutics
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Biomimetic NPs have emerged as innovative natural mimicking biosystems that can be used for a wide range of biomedical applications. ...
The development of vaccines plays a vital role in the effective control of several fatal diseases. ...
Conflicts of Interest: The authors declare no conflict of interest. ...
doi:10.3390/pharmaceutics11100534
pmid:31615112
pmcid:PMC6835828
fatcat:qeeryxkr45cfbl7xtdcfxgxhoq
Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins
2020
Advances in Polymer Technology
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Therefore, besides analyzing the state of the art in affinity separation strategies for PEGylated molecules, this review proposes a basic guideline for the selection of adequate ligands to provide information ...
In recent years, the effective purification of PEGylated therapeutic proteins from reaction media has received particular attention. ...
Another alternative for the purification of PEGylated proteins is affinity precipitation. is technique can be defined as the specific and scalable solid-liquid separation of an insoluble substance (precipitate ...
doi:10.1155/2020/6163904
fatcat:4ionynvvvng7jnz2lxnxfzf4ue
Preparative Purification of Recombinant Proteins: Current Status and Future Trends
2013
BioMed Research International
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Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous ...
Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols ...
Acknowledgments This study was supported by funding from the Science Foundation Ireland (SFI), Health Research Board of Ireland (HRB), and the Office of the Vice President of Research (OVPR), Dublin City ...
doi:10.1155/2013/312709
pmid:24455685
pmcid:PMC3877584
fatcat:vkjdtfs4sffmdg6xn2x4afg5h4
Technology advancements in antibody purification
2016
Antibody Technology Journal
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The purification of antibodies using chromatography involves the separation of antibodies or antibody-derived molecules present in complex mixtures by passing them through a solid phase (eg, silica resin ...
This review details current chromatographic and non-chromatographic methodo logies used for the purification of antibodies and expands on technological advancements and practical uses that have recently ...
Acknowledgments
Disclosure The authors report no conflicts of interest in this work. ...
doi:10.2147/anti.s64762
fatcat:hzillmbag5betkmyx6uzzlgvhq
Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides
2016
Materials
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Protein A, G, and L, surface proteins expressed by pathogenic bacteria, are well known to bind immunoglobulin and have been widely exploited in antibody purification strategies. ...
Besides their use as high affinity ligands in antibody purification chromatography, Fc-binding peptides are applied e.g., to localize antibodies on nanomaterials and to increase the half-life of proteins ...
Conflicts of Interest: The authors declare no conflict of interest. ...
doi:10.3390/ma9120994
pmid:28774114
pmcid:PMC5456964
fatcat:koid5udcebbpzeppblhapjih3e
Overview of Albumin and Its Purification Methods
2016
Advanced Pharmaceutical Bulletin
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It has a key role in oncotic pressure maintenance and it is known as a versatile protein carrier for transportation of various endogenous and exogenous ligands. ...
In this review, an overview of serum albumin and different purification methods are summarized. Mini Review 496 | Advanced Pharmaceutical Bulletin, 2016, 6(4), 495-507 Raoufinia et al. ...
CNBr may be a most proper matrices for protein purification due to working as an activation reagent. We produced HSA with a purity of 98% through CNBractivated sepharose. ...
doi:10.15171/apb.2016.063
pmid:28101456
pmcid:PMC5241407
fatcat:epsx5evrrvg3ji7ha4gxch2wra
Bioinspired and sustainable chitosan-based monoliths for antibody capture and release
2012
RSC Advances
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Chitosan-based monoliths activated by plasma technology induced the coupling of a biomimetic ligand and exhibited remarkable performance for the one-step capture and recovery of antibodies. ...
Please e-mail a list of corrections or the PDF with electronic notes attached -do not change the text within the PDF file or send a revised manuscript. ...
biomimetic affinity ligand, previously reported as an artificial Protein A for antibody purification. ...
doi:10.1039/c2ra21687f
fatcat:c4fg6d274zdvrgh2ftzyj53z2u
Molecular Imprinting Technology for Biomimetic Assemblies
2020
Hacettepe Journal of Biology and Chemistry
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However, in the last two decades molecularly imprinted polymers, MIPs, have been synthesized as an alternative to mimic natural biological interactions for a broad spectrum of templates by means of coordinating ...
A B S T R A C T T he term biomimetic can be simply defined as the examination of nature. ...
One of the best known biomimetic ligands are polymeric brushes can be used purification and separation processes. ...
doi:10.15671/hjbc.801427
fatcat:semmsb7wxvfjpmsy4jxebi4eum
Challenges and opportunities in the purification of recombinant tagged proteins
2014
Biotechnology Advances
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There is a wide range of available affinity pairs "tag-ligand" combining biological or structural affinity ligands with the respective binding tags. ...
This review gives a general overview of the well-established "tag-ligand" systems available for fusion protein purification and also explores current unconventional strategies under development. ...
structural ligand (metal chelate such as iminodiacetic acid cheated to Ni 2+ ) and (iii) a synthetic biomimetic ligand (ligand A3C1 specific for immunoglobulins (Haigh et al., 2009 ). ...
doi:10.1016/j.biotechadv.2013.12.001
pmid:24334194
pmcid:PMC7125906
fatcat:kol5ehaemnfldnyilaqvob34xm
Advances in targeted nanotherapeutics: From bioconjugation to biomimicry
2018
Nano Reseach
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Here, we provide a summary of each strategy, discuss considerations for their design moving forward, and highlight their potential clinical impact on cancer therapy. ...
These biomimetic NPs fall into two distinct categories: synthetic NPs that present naturally occurring structures, and NPs that are completely disguised by natural structures. ...
A final consideration for the clinical impact of targeted NPs is that the cost of production is often higher than non-targeted NPs or free ligands due to losses during NP synthesis and purification. ...
doi:10.1007/s12274-018-2083-z
pmid:31772723
pmcid:PMC6879063
fatcat:z65jukupwfb75k5wxhevie6ivy
Lecture abstracts
1995
Journal of Molecular Recognition
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This work will be discussed with reference to a variety of examples where ‘biomimetic’ ligands have been developed for the purification of particular proteins.
202 11TH ACBR—LECTURE ABSTRACTS
L-3-1 BIORECOGNITION ...
As an alternative to natural compounds, synthetic ligands (such as immobilised dye-ligands) have many endearing properties. ...
doi:10.1002/jmr.300080305
fatcat:ogn6ke2rufb6rmbthbcz44z2xa
Poster abstrcts
1995
Journal of Molecular Recognition
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flavo-proteins with biomimetic dye-ligands. ...
stoichiometry of binding site(s) between structurally selected biomimetic ligands and the apoenzyme were obtained. ...
doi:10.1002/jmr.300080306
fatcat:rwy3omrwvfb33l7xp437dexvna
Affinity monolith chromatography
2006
Journal of Separation Science
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Review Affinity monolith chromatography The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography ...
Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes ...
There are several examples in which pro-tein A or protein G has been used on GMA/EDMA disks or rods for the purification of antibodies [40, 41, 76, 85] . ...
doi:10.1002/jssc.200600152
pmid:16970180
fatcat:5udo24th4bavhnousn25edy72u
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