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A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand

Su Fern Teng, Kenny Sproule, Abid Hussain, Christopher R. Lowe
1999 Journal of Molecular Recognition  
A. (1992). Designer dyes. Biomimetic ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol. 10, 442-448. McLoughlin, S. B. and Lowe, C. R. (1988).  ...  JOURNAL OF MOLECULAR RECOGNITION J. Mol. Recognit. 1999;12:67—75 A strategy for the generation of biomimetic ligands for affinity chromatography.  ... 
doi:10.1002/(sici)1099-1352(199901/02)12:1<67::aid-jmr443>3.0.co;2-4 pmid:10398398 fatcat:m7xvzxxyizhvlglt53bt6gzb2y

Purification of antibodies by affinity chromatography

Klaus Huse, Hans-Joachim Böhme, Gerhard H Scholz
2002 Journal of Biochemical and Biophysical Methods  
This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies.  ...  The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described. D  ...  That is because Protein A has some important virtues for application as an affinity ligand.  ... 
doi:10.1016/s0165-022x(02)00017-9 pmid:12088882 fatcat:x7d6w3totzc2pnbopyrt2gpife

Recent Advances in Nanovaccines Using Biomimetic Immunomodulatory Materials

Vijayan, Mohapatra, Uthaman, Park
2019 Pharmaceutics  
Biomimetic NPs have emerged as innovative natural mimicking biosystems that can be used for a wide range of biomedical applications.  ...  The development of vaccines plays a vital role in the effective control of several fatal diseases.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/pharmaceutics11100534 pmid:31615112 pmcid:PMC6835828 fatcat:qeeryxkr45cfbl7xtdcfxgxhoq

Perspectives, Tendencies, and Guidelines in Affinity-Based Strategies for the Recovery and Purification of PEGylated Proteins

Luis Alberto Mejía-Manzano, Patricia Vázquez-Villegas, José González-Valdez
2020 Advances in Polymer Technology  
Therefore, besides analyzing the state of the art in affinity separation strategies for PEGylated molecules, this review proposes a basic guideline for the selection of adequate ligands to provide information  ...  In recent years, the effective purification of PEGylated therapeutic proteins from reaction media has received particular attention.  ...  Another alternative for the purification of PEGylated proteins is affinity precipitation. is technique can be defined as the specific and scalable solid-liquid separation of an insoluble substance (precipitate  ... 
doi:10.1155/2020/6163904 fatcat:4ionynvvvng7jnz2lxnxfzf4ue

Preparative Purification of Recombinant Proteins: Current Status and Future Trends

Mayank Saraswat, Luca Musante, Alessandra Ravidá, Brian Shortt, Barry Byrne, Harry Holthofer
2013 BioMed Research International  
Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous  ...  Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols  ...  Acknowledgments This study was supported by funding from the Science Foundation Ireland (SFI), Health Research Board of Ireland (HRB), and the Office of the Vice President of Research (OVPR), Dublin City  ... 
doi:10.1155/2013/312709 pmid:24455685 pmcid:PMC3877584 fatcat:vkjdtfs4sffmdg6xn2x4afg5h4

Technology advancements in antibody purification

Richard O'Kennedy, Caroline Murphy, Tatyana Devine
2016 Antibody Technology Journal  
The purification of antibodies using chromatography involves the separation of antibodies or antibody-derived molecules present in complex mixtures by passing them through a solid phase (eg, silica resin  ...  This review details current chromatographic and non-chromatographic methodo logies used for the purification of antibodies and expands on technological advancements and practical uses that have recently  ...  Acknowledgments Disclosure The authors report no conflicts of interest in this work.  ... 
doi:10.2147/anti.s64762 fatcat:hzillmbag5betkmyx6uzzlgvhq

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides

Weonu Choe, Trishaladevi Durgannavar, Sang Chung
2016 Materials  
Protein A, G, and L, surface proteins expressed by pathogenic bacteria, are well known to bind immunoglobulin and have been widely exploited in antibody purification strategies.  ...  Besides their use as high affinity ligands in antibody purification chromatography, Fc-binding peptides are applied e.g., to localize antibodies on nanomaterials and to increase the half-life of proteins  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/ma9120994 pmid:28774114 pmcid:PMC5456964 fatcat:koid5udcebbpzeppblhapjih3e

Overview of Albumin and Its Purification Methods

Ramin Raoufinia, Ali Mota, Neda Keyhanvar, Fatemeh Safari, Sara Shamekhi, Jalal Abdolalizadeh
2016 Advanced Pharmaceutical Bulletin  
It has a key role in oncotic pressure maintenance and it is known as a versatile protein carrier for transportation of various endogenous and exogenous ligands.  ...  In this review, an overview of serum albumin and different purification methods are summarized. Mini Review 496 | Advanced Pharmaceutical Bulletin, 2016, 6(4), 495-507 Raoufinia et al.  ...  CNBr may be a most proper matrices for protein purification due to working as an activation reagent. We produced HSA with a purity of 98% through CNBractivated sepharose.  ... 
doi:10.15171/apb.2016.063 pmid:28101456 pmcid:PMC5241407 fatcat:epsx5evrrvg3ji7ha4gxch2wra

Bioinspired and sustainable chitosan-based monoliths for antibody capture and release

Telma Barroso, Ana C. A. Roque, Ana Aguiar-Ricardo
2012 RSC Advances  
Chitosan-based monoliths activated by plasma technology induced the coupling of a biomimetic ligand and exhibited remarkable performance for the one-step capture and recovery of antibodies.  ...  Please e-mail a list of corrections or the PDF with electronic notes attached -do not change the text within the PDF file or send a revised manuscript.  ...  biomimetic affinity ligand, previously reported as an artificial Protein A for antibody purification.  ... 
doi:10.1039/c2ra21687f fatcat:c4fg6d274zdvrgh2ftzyj53z2u

Molecular Imprinting Technology for Biomimetic Assemblies

Nilay BERELİ, Semra AKGÖNÜLLÜ, Sevgi ASLIYÜCE, Duygu ÇİMEN, İ̇lgim GÖKTÜRK, Deniz TÜRKMEN, Handan YAVUZ, Adil DENİZLİ
2020 Hacettepe Journal of Biology and Chemistry  
However, in the last two decades molecularly imprinted polymers, MIPs, have been synthesized as an alternative to mimic natural biological interactions for a broad spectrum of templates by means of coordinating  ...  A B S T R A C T T he term biomimetic can be simply defined as the examination of nature.  ...  One of the best known biomimetic ligands are polymeric brushes can be used purification and separation processes.  ... 
doi:10.15671/hjbc.801427 fatcat:semmsb7wxvfjpmsy4jxebi4eum

Challenges and opportunities in the purification of recombinant tagged proteins

Ana Sofia Pina, Christopher R. Lowe, Ana Cecília A. Roque
2014 Biotechnology Advances  
There is a wide range of available affinity pairs "tag-ligand" combining biological or structural affinity ligands with the respective binding tags.  ...  This review gives a general overview of the well-established "tag-ligand" systems available for fusion protein purification and also explores current unconventional strategies under development.  ...  structural ligand (metal chelate such as iminodiacetic acid cheated to Ni 2+ ) and (iii) a synthetic biomimetic ligand (ligand A3C1 specific for immunoglobulins (Haigh et al., 2009 ).  ... 
doi:10.1016/j.biotechadv.2013.12.001 pmid:24334194 pmcid:PMC7125906 fatcat:kol5ehaemnfldnyilaqvob34xm

Advances in targeted nanotherapeutics: From bioconjugation to biomimicry

Danielle M. Valcourt, Jenna Harris, Rachel S. Riley, Megan Dang, Jianxin Wang, Emily S. Day
2018 Nano Reseach  
Here, we provide a summary of each strategy, discuss considerations for their design moving forward, and highlight their potential clinical impact on cancer therapy.  ...  These biomimetic NPs fall into two distinct categories: synthetic NPs that present naturally occurring structures, and NPs that are completely disguised by natural structures.  ...  A final consideration for the clinical impact of targeted NPs is that the cost of production is often higher than non-targeted NPs or free ligands due to losses during NP synthesis and purification.  ... 
doi:10.1007/s12274-018-2083-z pmid:31772723 pmcid:PMC6879063 fatcat:z65jukupwfb75k5wxhevie6ivy

Lecture abstracts

1995 Journal of Molecular Recognition  
This work will be discussed with reference to a variety of examples where ‘biomimeticligands have been developed for the purification of particular proteins. 202 11TH ACBR—LECTURE ABSTRACTS L-3-1 BIORECOGNITION  ...  As an alternative to natural compounds, synthetic ligands (such as immobilised dye-ligands) have many endearing properties.  ... 
doi:10.1002/jmr.300080305 fatcat:ogn6ke2rufb6rmbthbcz44z2xa

Poster abstrcts

1995 Journal of Molecular Recognition  
flavo-proteins with biomimetic dye-ligands.  ...  stoichiometry of binding site(s) between structurally selected biomimetic ligands and the apoenzyme were obtained.  ... 
doi:10.1002/jmr.300080306 fatcat:rwy3omrwvfb33l7xp437dexvna

Affinity monolith chromatography

Rangan Mallik, David S. Hage
2006 Journal of Separation Science  
Review Affinity monolith chromatography The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography  ...  Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes  ...  There are several examples in which pro-tein A or protein G has been used on GMA/EDMA disks or rods for the purification of antibodies [40, 41, 76, 85] .  ... 
doi:10.1002/jssc.200600152 pmid:16970180 fatcat:5udo24th4bavhnousn25edy72u
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