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Structural determination of Streptococcus pyogenes M1 protein interactions with human immunoglobulin G using integrative structural biology [article]

Hamed Khakzad, Lotta Happonen, Yasaman Karami, Michael Nilges, Guy Tran Van Nhieu, Johan Malmström, Lars Malmstroem
2020 bioRxiv   pre-print
Here, we characterized the structure and dynamics of this interaction interface in human plasma on the surface of live bacteria using integrative structural biology, combining cross-linking mass spectrometry  ...  Both these interactions mimic the protein G-IgG interface of group C and G streptococcus.  ...  Here, we describe the interaction of the M1 protein, an important virulence factor of GAS, with human immunoglobulin G (IgG1-4) using targeted cross-linking mass spectrometry combined with molecular dynamic  ... 
doi:10.1101/2020.07.21.213652 fatcat:ur53hznyinbhfkxg3icqdmglie

Structural determination of Streptococcus pyogenes M1 protein interactions with human immunoglobulin G using integrative structural biology

Hamed Khakzad, Lotta Happonen, Yasaman Karami, Sounak Chowdhury, Gizem Ertürk Bergdahl, Michael Nilges, Guy Tran Van Nhieu, Johan Malmström, Lars Malmström, Alexey Onufriev
2021 PLoS Computational Biology  
Here, we characterized the structure and dynamics of this interaction interface in human plasma on the surface of live bacteria using integrative structural biology, combining cross-linking mass spectrometry  ...  Both these interactions mimic the protein G-IgG interface of group C and G streptococcus.  ...  Here, we describe the interaction of the M1 protein, an important virulence factor of GAS, with human immunoglobulin G (IgG1-4) using targeted cross-linking mass spectrometry combined with molecular dynamic  ... 
doi:10.1371/journal.pcbi.1008169 pmid:33411763 fatcat:6ekj6npqkredreloqjlplgwlpa

Conserved and specific features of Streptococcus pyogenes and Streptococcus agalactiae transcriptional landscapes

Isabelle Rosinski-Chupin, Elisabeth Sauvage, Agnès Fouet, Claire Poyart, Philippe Glaser
2019 BMC Genomics  
Availability of data and materials The complete genome sequence and annotation were deposited under accession number PRJEB27939 ERS2625773 ERZ778133, Genbank accession number LR031521. 1  ...  Funding This work was supported by grants from the French National Research Agency LabEx IBEID and by the High Council for Scientific and Technological Cooperation between France-Israel "Complexity in Biology  ...  There was an up-regulation of the gene coding for the immunoglobulin G binding protein Grab, of the genes involved in the synthesis of the pilus (SP119_0103-0107) and of the gene encoding the laminin-binding  ... 
doi:10.1186/s12864-019-5613-5 fatcat:6vwi6ywstzaxlizrvhau7eigyu

Streptococcus Adherence and Colonization

A. H. Nobbs, R. J. Lamont, H. F. Jenkinson
2009 Microbiology and Molecular Biology Reviews  
ACKNOWLEDGMENTS We thank members of our laboratories for their excellent work on many aspects of Streptococcus molecular genetics and for their helpful discussions.  ...  Owing to the complexity of this area of research, there are undoubtedly references that we have inadvertently not cited, or not been able to cite, for which we sincerely apologize.  ...  The interaction with protein G ablates the ability of IgG to bind C1q, thus preventing the activation of the classical pathway of complement.  ... 
doi:10.1128/mmbr.00014-09 pmid:19721085 pmcid:PMC2738137 fatcat:dfi4wc4ybrg5pcpyb7geranvde

Genome Sequence of a Nephritogenic and Highly Transformable M49 Strain of Streptococcus pyogenes

W. M. McShan, J. J. Ferretti, T. Karasawa, A. N. Suvorov, S. Lin, B. Qin, H. Jia, S. Kenton, F. Najar, H. Wu, J. Scott, B. A. Roe (+1 others)
2008 Journal of Bacteriology  
The 1,815,783-bp genome of a serotype M49 strain of Streptococcus pyogenes (group A streptococcus [GAS]), strain NZ131, has been determined.  ...  As with the previously sequenced S. pyogenes genomes, three unique prophages are a major source of genetic diversity.  ...  We acknowledge the use of the S. pyogenes MLST database that is located at Imperial College London and is funded by the Wellcome Trust.  ... 
doi:10.1128/jb.00672-08 pmid:18820018 pmcid:PMC2583620 fatcat:jyj4jvyke5em7gymrfiyefp7si

Expression and characterization of group A Streptococcus extracellular cysteine protease recombinant mutant proteins and documentation of seroconversion during human invasive disease episodes

S Gubba, D E Low, J M Musser
1998 Infection and Immunity  
A recent study with isogenic strains constructed by recombinant DNA strategies unambiguously documented that a highly conserved extracellular cysteine protease expressed by Streptococcus pyogenes (group  ...  The recombinant C192S zymogen retained apparently normal structural integrity, as assessed by the ability of purified wild-type streptococcal cysteine protease to process the 40-kDa molecule to the 28-  ...  The enzyme degrades human fibronectin and vitronectin (15) , two proteins involved in maintaining the integrity of the extracellular matrix and cell-cell interactions, and activates interleukin-1␤ precursor  ... 
pmid:9453639 pmcid:PMC107968 fatcat:hr2w2fbubjgbpihaqf5zbitrma

The AgI/II Family Adhesin AspA Is Required for Respiratory Infection by Streptococcus pyogenes

Linda Franklin, Angela H. Nobbs, Laura Bricio-Moreno, Christopher J. Wright, Sarah E. Maddocks, Jaspreet Singh Sahota, Joe Ralph, Matthew O'Connor, Howard F. Jenkinson, Aras Kadioglu, Sean D. Reid
2013 PLoS ONE  
Streptococcus pyogenes (GAS) is a human pathogen that causes pharyngitis and invasive diseases such as toxic shock syndrome and sepsis.  ...  We describe a novel mouse model of GAS colonization of the nasopharynx and lower respiratory tract to elucidate these interactions.  ...  Acknowledgments We thank Jane Brittan and Lindsay Dutton for excellent technical assistance, Sylvester Emmanuel Vin-Kab for helping with assays, Tim Foster for providing pKS80, and Andreas Podbielski for  ... 
doi:10.1371/journal.pone.0062433 pmid:23638083 pmcid:PMC3640068 fatcat:qpurkqyknrd5jjiro2hslpzary

The Seventh International Conference on the Genetics of Streptococci, Lactococci, and Enterococci

R. A. Burne, D. E. Bessen, J. R. Broadbent, J.-P. Claverys
2006 Journal of Bacteriology  
of the tremendous insights gained through genetic analysis of this unique group of eubacteria. complete paragraph, the last three sentences should be replaced with the following: "Newly appearing type  ...  19A capsule and flanking penicillin-binding protein genes from an ST199 donor strain to a serotype 4 ST695 recipient strain.  ...  ACKNOWLEDGMENTS We thank Lisa Nalker and Latonya Nichols of the American Society for Microbiology for their hard work, dedication, and professionalism.  ... 
doi:10.1128/jb.01363-06 pmid:17012391 pmcid:PMC1797339 fatcat:5pvd3xnicjcffieyjrewinzpcu

Phylogenomic analysis of natural selection pressure in Streptococcus genomes

Maria Anisimova, Joseph Bielawski, Katherine Dunn, Ziheng Yang
2007 BMC Evolutionary Biology  
The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal lineages, and we relate our findings to the pre-existing knowledge of Streptococcus virulence factors.  ...  Results: Our analysis of 1730 gene clusters revealed 136 cases of positive Darwinian selection, which we suggest is most likely to result from an antagonistic interaction between the host and pathogen  ...  MA was supported by a fellowship from BBSRC, and KAD was supported in part by fellowships from Genome Canada and the Natural Sciences and Engineering Research Council of Canada.  ... 
doi:10.1186/1471-2148-7-154 pmid:17760998 pmcid:PMC2031904 fatcat:pkdtfqngdfgedn3oui6evsn27y

Disease Manifestations and Pathogenic Mechanisms of Group A Streptococcus

M. J. Walker, T. C. Barnett, J. D. McArthur, J. N. Cole, C. M. Gillen, A. Henningham, K. S. Sriprakash, M. L. Sanderson-Smith, V. Nizet
2014 Clinical Microbiology Reviews  
Over 100 years later, a safe and effective com-mercial vaccine against Streptococcus pyogenes (group A Streptococcus [GAS]) is still not licensed for human use (2) (3) (4) .  ...  Upregulation of capsule enables Streptococcus pyogenes to evade immune recognition by antigen-specific antibodies directed to the G-related alpha2-macroglobulin-binding protein GRAB located on the bacterial  ... 
doi:10.1128/cmr.00101-13 pmid:24696436 pmcid:PMC3993104 fatcat:d5d5qou7q5gmzm5qxqckpk27yi

Mode of Expression and Functional Characterization of FCT-3 Pilus Region-Encoded Proteins in Streptococcus pyogenes Serotype M49

M. Nakata, T. Koller, K. Moritz, D. Ribardo, L. Jonas, K. S. McIver, T. Sumitomo, Y. Terao, S. Kawabata, A. Podbielski, B. Kreikemeyer
2008 Infection and Immunity  
The human pathogen Streptococcus pyogenes (group A streptococcus [GAS]) pilus components, suggested to play a role in pathogenesis, are encoded in the variable FCT (fibronectin-and collagen-binding T-antigen  ...  the old poles of GAS cells, (ii) FctA protein expression increased with lower temperatures, and (iii) FctA protein expression was restricted to 20 to 50% of a given GAS M49 population, suggesting regulation  ...  B.K. and A.P. further acknowledge support by BMBF grants 0313936A and 0313979B within the framework of the SysMo Systems Biology of Microorganisms and ERANet Pathogenomics competence networks, respectively  ... 
doi:10.1128/iai.00772-08 pmid:18852238 pmcid:PMC2612252 fatcat:a2wkszwihbbgxaxuvjgpox23xe

Transcriptomic profiling of Streptococcus pyogenes M1T1 strain in a mouse model of necrotizing fasciitis [article]

Yujiro Hirose, Masaya Yamaguchi, Daisuke Okuzaki, Daisuke Motooka, Hiroshi Hamamoto, Tomoki Hanada, Tomoko Sumitomo, Masanobu Nakata, Shigetada Kawabata
2019 bioRxiv   pre-print
Streptococcus pyogenes is a major cause of necrotizing fasciitis, a life-threatening subcutaneous soft-tissue infection.  ...  In this study, we used a mouse model of necrotizing fasciitis and performed RNA-sequencing (RNA-seq) analysis of S. pyogenes M1T1 strain 5448 by using infected hindlimbs obtained at 24, 48, and 96 h post-infection  ...  Streptococcus pyogenes : Basic Biology to Clinical 421 Manifestations. Oklahoma City (OK)2016. 422 2.  ... 
doi:10.1101/610139 fatcat:nzi3dukl3bhgpiip6nfsnc4cum

Pathogenesis of Group A Streptococcal Infections

Madeleine W. Cunningham
2000 Clinical Microbiology Reviews  
The emm gene superfamily has expanded to include antiphagocytic molecules and immunoglobulin-binding proteins with common structural features.  ...  A resurgence of invasive streptococcal diseases and rheumatic fever has appeared in outbreaks over the past 10 years, with a predominant M1 serotype as well as others identified with the outbreaks. emm  ...  ACKNOWLEDGMENTS Deepest appreciation is expressed to all of my colleagues who helped make this review possible by sending their reprints and providing comments on portions of the review.  ... 
doi:10.1128/cmr.13.3.470 fatcat:pyahfnmh4bdhdfo3rshs5ugodi

Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion

H. Brussow, C. Canchaya, W.-D. Hardt
2004 Microbiology and Molecular Biology Reviews  
ACKNOWLEDGMENTS We are grateful to Cosima Pelludat for critical reading of the manuscript and to Anne Bruttin for help with the editorial work.  ...  S. pyogenes has fascinated medical microbiologists for nearly a century. It is a protean pathogen, and humans are its only reservoir. One-third of all humans are colonized with S. pyogenes.  ...  from reference 132 with permission. b Genes located on pathogenicity islands are in bold; those on prophages are in bold and italics. c IgG, immunoglobulin G.592BRÜ SSOW ET AL.  ... 
doi:10.1128/mmbr.68.3.560-602.2004 pmid:15353570 pmcid:PMC515249 fatcat:fyb6m44rtjfgnm4a6jwbkgxx2y

The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

Pierre Nottelet, Laure Bataille, Geraldine Gourgues, Robin Anger, Carole Lartigue, Pascal Sirand-Pugnet, Esther Marza, Remi Fronzes, Yonathan Arfi
2021 Science Advances  
Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave  ...  These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools  ...  MIB tightly interacts with the immunoglobulin G (IgG) Fab domain and subsequently recruits the serine protease MIP.  ... 
doi:10.1126/sciadv.abf2403 pmid:33674316 pmcid:PMC7935358 fatcat:7qxswclywfennfehrchwigkcgi
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