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Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay

Song Cheng, Ming-hui Chen, Gang-gang Zhang, Zhi-biao Yu, Dao-feng Liu, Yong-hua Xiong, Hua Wei, Wei-hua Lai
2017 Sensors  
A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples.  ...  Since C. freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/s17040753 pmid:28368342 pmcid:PMC5421713 fatcat:njdwrbeds5fujhlncwot3rxpt4

Nanomaterial-based sensors for detection of foodborne bacterial pathogens and toxins as well as pork adulteration in meat products

B. Stephen Inbaraj, B.H. Chen
2016 Journal of Food and Drug Analysis  
In addition, the application through a noncross-linking method without the need for any surface modification is also presented for detection of pork adulteration in meat products.  ...  In recent years, nanotechnology has emerged as a promising field for solving food safety issues in terms of detecting contaminants, enabling controlled release of preservatives to extend the shelf life  ...  This resulted in a change in mass with a concomitant change in QCM frequency for detection of E. coli O157:H7.  ... 
doi:10.1016/j.jfda.2015.05.001 pmid:28911398 fatcat:tnmnqq6ypjghzmwvpqfl2mehjy

Recent Advances in Potential Nanoparticles and Nanotechnology for Sensing Food-Borne Pathogens and Their Toxins in Foods and Crops: Current Technologies and Limitations

2014 Sensors and materials  
Food-borne pathogens cause infectious diseases in people worldwide. Hence, the development of rapid detection methods for food-borne pathogens is necessary.  ...  The application of potentially useful nanoparticles (NPs) results in enhanced sensitivity, improved response time, and increased portability owing to their distinct chemical and optical features.  ...  This work was also supported by a grant from Mahidol University, Thailand, and by a grant from the Faculty of Environment and Resource Studies, Mahidol University, Thailand.  ... 
doi:10.18494/sam.2014.1015 fatcat:jgzjmkfuq5hkratnqunoj366qm

Biosensors: A Novel Approach to Detect Food-borne Pathogens

Esra Senturk, Simge Aktop, Pınar Sanlibaba, Basar Uymaz Tezel
2018 Applied Microbiology open access  
The use of biosensors is considered a new approach to quickly detect foodborne pathogens and their toxins.  ...  Immunological, molecular, and cultural methods are frequently used in the detection of foodborne pathogens.  ...  ELISA and lateral flow immunoassay are among the immunological methods that are currently used for the detection of foodborne pathogens [53] .  ... 
doi:10.4172/2471-9315.1000151 fatcat:el564xkxmzbebhbezyjgpfnwwa

In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering

Lu-Lu Qu, Yi-Lun Ying, Ru-Jia Yu, Yi-Tao Long
2021 Mikrochimica Acta  
The latest progress on the use of SERS for rapid detection of food-borne bacteria and viruses is considered, including both the labeled and label-free detection strategies.  ...  an effective detection strategy for pathogens.  ...  Declarations Conflict of interest The authors declare that they have no conflict of interest. Conflict of interest The authors declare that they have no competing of interests.  ... 
doi:10.1007/s00604-021-04864-4 pmid:34041602 pmcid:PMC8154335 fatcat:qf55qulnira4jgj4tx6bhq27si

A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food

I Abubakar, L Irvine, C Aldus, G Wyatt, R Fordham, S Schelenz, L Shepstone, A Howe, M Peck, P Hunter
2007 Health Technology Assessment  
Non-UK purchasers will have to pay a small fee for post and packing. For European countries the cost is £2 per monograph and for the rest of the world £3 per monograph.  ...  Comparison of the BAX for screening E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef.  ...  E. coli O157:H7 test for detection of Escherichia coli O157:H7 in stools Notes: 2 × 2 data derived from prospective evaluation only, to mirror diagnostic practice Setting: Multi-site evaluation, Canada  ... 
doi:10.3310/hta11360 pmid:17803865 fatcat:onavonjugra4jdcrq2zjxoexey

Recent Advancements in the Technologies Detecting Food Spoiling Agents

Reena V. Saini, Prachi Vaid, Neeraj K. Saini, Samarjeet Singh Siwal, Vijai Kumar Gupta, Vijay Kumar Thakur, Adesh K. Saini
2021 Journal of Functional Biomaterials  
The current review focuses on the advancements in these methods for the detection of food-borne contaminants and pathogens.  ...  In this contest, there is a dire necessity to comprehend, implement, and monitor the levels of contaminants by utilizing various available methods, such as single-cell droplet microfluidic system, DNA  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/jfb12040067 pmid:34940546 pmcid:PMC8709279 fatcat:lmrell5j6jbjjmnkqtstjwtsaq

Disposable Paper-Based Biosensors for the Point-of-Care Detection of Hazardous Contaminations—A Review

Mohammad Mahdi Bordbar, Azarmidokht Sheini, Pegah Hashemi, Ali Hajian, Hasan Bagheri
2021 Biosensors  
For liquid samples, various structures (including a dipstick, as well as microfluidic and lateral flow) have been constructed.  ...  sensor elements, and the connection between two methods of detection in a small set.  ...  Acknowledgments: The authors gratefully acknowledge Research Council of Baqiyatallah University of Medical Sciences. Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/bios11090316 pmid:34562906 fatcat:6g3xoj4x3bc2hp3fqmr4list2e

Sensitive Detection of Foodborne E. coli O157:H7 by Dendrimer-aptamer Modified Microchannels - with RCA Signal Intensifications [article]

Yuqian Jiang, University, My, University, My
Subsequently, the optimized RCA reaction conditions are used in a dendrimer-aptamer microfluidic whole-cell detection system for E. coli O157:H7 detection with signal RCA (sRCA) signal intensifications  ...  In situ cRCA synthesis on microchannel inner surface is characterized and optimized for its application in capturing E. coli O157:H7 cells.  ...  Using antibody based lateral flow immunoassay, Cho et al. recently described detection of E. coli O157:H7 with a LOD of 10 2 CFU/mL, which was completed within 15 min [41] .  ... 
doi:10.20381/ruor-22028 fatcat:xwfza5shyre77ejitpfsgkrlbm

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

Aldert A. Bergwerff, Sylvia B. Debast
2021 Foods  
Containment requires easy-to-use, failsafe, single-use tests giving an overall risk score in situ.  ...  The context is that the highest risk of food contamination is through the animal and human fecal route with a majority of foodborne infections originating from sources in mass and domestic kitchens at  ...  The detection of Escherichia coli O157:H7 in ground beef is an example of a direct bacterial application [144] .  ... 
doi:10.3390/foods10040832 pmid:33920486 fatcat:xayt5yzl6zeprooqhhpvkoyuoa

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Listeria monocytogenes

Wang Y, Li H, Wang Y, Li H, Luo L, Xu J, Ye C
2017 International Journal of Nanomedicine  
In this report, we propose a simple, rapid, and nearly instrument-free molecular technique using multiple cross displacement amplification (MCDA) label-based gold nanoparticles lateral flow biosensor (  ...  The assay was also successfully applied to detecting L. monocytogenes in pork samples.  ...  A rapid and highly sensitive protocol for the detection of Escherichia coli O157: H7 based on immunochromatography assay combined with the enrichment technique of immunomagnetic nanoparticles.  ... 
doaj:4a684e0a26e14156ad9d45d4f219c2a5 fatcat:tk7hsfq76zau7p6coeyx33qu74

Ensuring food safety using aptamer based assays: Electroanalytical approach

Hedieh Malekzad, Abolghasem Jouyban, Mohammad Hasanzadeh, Nasrin Shadjou, Miguel de la Guardia
2017 TrAC. Trends in analytical chemistry  
analytical solutions for determination of health threatening contaminants in all stages of production process, is the next field of research for developing efficient electrochemical aptasensors.  ...  Aptamers, are being increasingly employed as favorable receptors for constructing highly sensitive biosensors, for their remarkable affinities towards certain targets including a wide scope of biological  ...  as a model for E. coli O157: H7.  ... 
doi:10.1016/j.trac.2017.07.001 pmid:32287541 pmcid:PMC7112916 fatcat:oekxai5q4jbbtm5ftzntcdcgje

Multivariate Markovian Modeling of Tuberculosis: Forecast for the United States

Sara Debanne
2000 Emerging Infectious Diseases  
We report a case of chronic Schistosoma haematobium infection with pseudometastatic pulmonary nodules and high-grade squamous cell carcinoma in a 30-year-old man in Mali.  ...  This work was supported in part by a grant from the Norwegian Ministry of Agriculture. About the Author Dr. Pirisinu is a researcher at the Istituto Superiore di Sanità in Rome, Italy.  ...  We used whole-genome sequencing to investigate the evolutionary context of an emerging highly pathogenic strain of Shiga toxin-producing Escherichia coli (STEC) O157:H7 in England and Wales.  ... 
doi:10.3201/eid0602.000207 pmid:10756148 pmcid:PMC2640843 fatcat:772hgyrffffprdrgyiwb7urehe

Pseudomembranous colitis

Priya D. Farooq, Nathalie H. Urrunaga, Derek M. Tang, Erik C. von Rosenvinge
2015 Disease-a-month  
The less common non-C. difficile causes of pseudomembranous colitis should be entertained, as a number of etiologies can result in this condition.  ...  Examples include Behcet's disease, collagenous colitis, inflammatory bowel disease, ischemic colitis, other infections organisms (e.g. bacteria, parasites, viruses), and a handful of drugs and toxins.  ...  Enterohemorrhagic Escherichia coli O157:H7 Escherichia coli is a gram-negative, facultative-anaerobic, rod-shaped bacterium that is ubiquitous in the environment and in humans and is associated with a  ... 
doi:10.1016/j.disamonth.2015.01.006 pmid:25769243 pmcid:PMC4402243 fatcat:bspmj4zgundmpknzxfdu5uscvq

Recent Applications of Magnetic Nanoparticles in Food Analysis

Bárbara Socas-Rodríguez, Antonio V. Herrera-Herrera, María Asensio-Ramos, Miguel Ángel Rodríguez-Delgado
2020 Processes  
Nanotechnology has become a topic of interest due to the outstanding advantages that the use of nanomaterials offers in many fields.  ...  The aim of this review article is to summarise the most remarkable applications of m-NPs in food analysis in the last five years (2016–2020) showing a general view of the use of such materials in the field  ...  Conflicts of Interest: The authors declare no conflict of interest. Abbreviations  ... 
doi:10.3390/pr8091140 fatcat:zc3fk3u6qfdbdf3sa32uoswm2u
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