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, un punto di riferimento obbligato, ogniqualvólta si voglia parlare di identificazione di partito; si tratterà poi di stabilire se la nozione di identificazione a cui si fa riferimento possa ancora essere ... considerata conforme con l'originale costrutto, oppure ci si trovi di fronte ad un nuovo concetto, differente e non riconducibile al primo. ... Non c'e dubbio che molte somiglianze permangono tra i due tipi di at-374 Stefano Palma tori. ...doi:10.1017/s0048840200022279 fatcat:oznkj4hi6faazlpc3e5cizqeti
In this work, two procedures for fabrication of polymeric microneedles based on direct photolithography, without any etching or molding process, are reported. Polyethylene glycol (average molecular weight 250 Da), casted into a silicone vessel and exposed to ultraviolet light (365 nm) through a mask, cross-links when added by a commercial photocatalyzer. By changing the position of the microneedles support with respect to the vessel, different shapes and lengths can be achieved. Microneedlesdoi:10.3390/ma8125484 pmid:28793736 pmcid:PMC5458808 fatcat:34r3od7sqzf6xp4gyi6j42atp4
more »... m a hundred microns up to two millimeters have been obtained just tuning the radiation dose, by changing the exposure time (5-15 s) and/or the power density (9-18 mW/cm 2 ) during photolithography. Different microneedle shapes, such as cylindrical, conic or lancet-like, for specific applications such as micro-indentation or drug delivery, are demonstrated. Materials 2015, 8, 8661-8673 applications  . Many other biodegradable polymers have been extensively employed in MNs fabrication for drugs delivery application      . Hydrogels are attractive materials in fabrication of biodevices, since a hydrated gel provides an excellent matrix for encapsulation of functional biological molecules, such as enzymes and peptides [9, 10, 15, 16] . Main advantages are briefly summarized in the following: (a) device miniaturization by photolithographic patterning; (b) development of biocompatible tissue/sensor interface; (c) near-physiological conditions that minimize protein denaturation helping them to carry out their full biological functions; and (d) three-dimensional geometry of hydrogels enables them to contain large quantity of sensing reagent, thereby increasing their signal-to-noise ratio and sensitivity [16, 17] . Poly(ethylene glycol) diacrylate (PEGDA) is a biologically inert polymer that rapidly hardens at room temperature in presence of a photo-initiator on exposure to ultraviolet (UV) light, so that it is possible to fabricate PEGDA MNs by standard photolithographic process. PEGDA is hydrophilic, elastic and can be customized to include a variety of biological molecules or inorganic nanoparticles      . However, the type of target materials and size are critical factors in determination of microneedles applicability. Tailoring of size, shape and tip of MN is highly desirable. Generic photolithographic processes in microelectronic technology require that a substrate is covered by spin-coating a liquid photosensitive polymer, called photoresist, which is later hardened by a thermal process. Then, the photoresist is exposed to ultraviolet radiation through a mask, which reproduces the design of the structure to be realized. In our case, the sketch on the mask is the array of circles that constitute the base of needles. UV light creates or destructs "cross-link" bonds of the polymer, in case the resist is "negative" or "positive", respectively. In this way, the solubility of the exposed resist is drastically different from that in the shade of mask. By dipping the sample in the developing solution, the portion of the photoresist exposed (or not exposed, depending on whether the photoresist is negative or positive, respectively) is removed. As the last step, the so-called etching step, the drawing made of photoresist is then reproduced on the substrate with wet or dry chemical attack  . Kochhar et al. , proposed a method for MNs array fabrication based on a single step process, realizing MNs with truncated cone shape and variable length upper to 1 mm. In this work, we proposed two procedures (Method 1 and Method 2 in the following) of direct photolithography, where the mixture of PEGDA and DAROCUR © (BASF, Ludwigshafen am Rhein, Germany) has been used just like an ordinary photoresist and the etching step was not longer required to transfer the design on the substrate. The present photolithographic approach to fabricate PEGDA MNs, using microelectronic fabrication facility and skills, allows the realization of MNs with different shape, length and tip by changing the exposure parameters and the position of the support. The processes provide to a wide range of MN types, useful either for epidermis or dermis targeting. In fact, the needle morphological and dimensional features can be decided by adjusting the exposure parameters, depending on the specific application.
Data originating from open-source software projects provide valuable information to enhance software quality. In the scope of Software Defect Prediction, one of the most challenging parts is extracting valid data about failure-prone software components from these repositories, which can help develop more robust software. In particular, collecting data, calculating metrics, and synthesizing results from these repositories is a tedious and error-prone task, which often requires understanding thearXiv:2111.11807v1 fatcat:jlus7hlnvzbknccwv4acwzlbnq
more »... rogramming languages involved in the mined repositories, eventually leading to a proliferation of language-specific data-mining software. This paper presents RepoMiner, a language-agnostic tool developed to support software engineering researchers in creating datasets to support any study on defect prediction. RepoMiner automatically collects failure data from software components, labels them as failure-prone or neutral, and calculates metrics to be used as ground truth for defect prediction models. We present its implementation and provide examples of its application.
By exploiting the PMN property to produce high quantities of oxygen peroxide to neutralize pathogens, the oxygen peroxide content of uterine cells was measured to diagnose endometritis. After preliminary in vitro studies in which endometrial cells from slaughtered mares were mixed with leukocytes from peripheral blood, endometrial samples were collected by uterine flushing from mares before insemination. Staining endometrial cells with H2DCF-DA was combined with hydroethidine to normalize thedoi:10.3390/ani12091157 pmid:35565583 pmcid:PMC9101999 fatcat:76rh2p6ndbbuxlynm47ywtpose
more »... uorescence intensity with the cellular content of the sample. Stained cell smears were assumed as the gold standard of endometritis, and based on this assay, the samples were considered positive (C+) and negative (C−) for endometritis. The amount and the turbidity of fluid recovered by uterine flushing were significantly (p < 0.01) higher in C+ than in C−. Moreover, the oxygen peroxide content of the endometrial cells was significantly higher in the C+ than in the C− group (6.31 ± 1.92 vs. 3.12 ± 1.26, p = 0.001). Using the value of 4.4 as the cutoff level of this fluorescence cytology assay, it was found that only one C− sample exceeded the cutoff level (false positives = 7.7%) while three C+ samples showed values below the cutoff level (false negative = 11.5%).
O R C I D Konstantinos Rigas https://orcid.org/0000-0002-9125-5054 Stefano Di Palma https://orcid.org/0000-0002-9466-3918 On clinical examination, there was generalized erythema (Figure 1), peripheral ...doi:10.1002/vrc2.93 fatcat:6sa4xdjsy5fgvinfcaszavw5za
Supplemental Material, DS1_VET_10.1177_0300985818824952 for Inter- and Intraobserver Agreement of Canine and Feline Nervous System Tumors by Sara Belluco, Giancarlo Avallone, Stefano Di Palma, Roberta ...doi:10.25384/sage.7618286.v1 fatcat:grdeppwjqnczph7v3upnkptgyq
Linguistic anti-patterns are recurring poor practices concerning inconsistencies among the naming, documentation, and implementation of an entity. They impede readability, understandability, and maintainability of source code. This paper attempts to detect linguistic anti-patterns in infrastructure as code (IaC) scripts used to provision and manage computing environments. In particular, we consider inconsistencies between the logic/body of IaC code units and their names. To this end, we proposearXiv:2009.10801v1 fatcat:6ievblilkbdonkoh4afwhfwz6a
more »... a novel automated approach that employs word embeddings and deep learning techniques. We build and use the abstract syntax tree of IaC code units to create their code embedments. Our experiments with a dataset systematically extracted from open source repositories show that our approach yields an accuracy between0.785and0.915in detecting inconsistencies
Stefano, Dario, and Damian are supported by the European Commission grant no. 825040 (H2020 RADON). Fabio acknowledges the support of the Swiss National Science Foundation through the SNF Project No. ...arXiv:2005.13474v2 fatcat:u5x24cj2l5bcbgunnxxmaxr2ca
., among 99 patients hospitalized for COVID-19 in Northern Italy, reported an overall prevalence of 19%, which increases to 36% in those with cardiovascular diseases and 42% among patients who died during ... (B).Among overall study population, 124 patients (26.5%) presented with severe ARDS at admission to the emergency department, 169 patients (36.2%) developed severe ARDS during hospitalization and 107 died ...doi:10.3390/medicina58030399 pmid:35334575 pmcid:PMC8951344 fatcat:22twxcnqnjgi3gqjrzwlpf6odi
Copyright © 2019 Ferrari, Satta, Palma, Di Mario, Catone, O'Keeffe, Zema, Prosperi and Turchini. ...doi:10.3389/fchem.2019.00348 pmid:31165061 pmcid:PMC6536591 fatcat:uz34lzi5zbdjnecgmzjhb6ielq
Foreign body aspiration is common in the pediatric age group, especially in males. Despite the high frequency of this potentially life-threatening event, it is not always easy to recognize it given the high variability of the clinical presentation and the potential of "pauci-symptomatic" inhalation. Moreover, a variable latency of the onset of symptoms since the moment of aspiration may be possible determining difficulties in the identification of the inhalation on an anamnestic basis. Wedoi:10.3390/pediatric14010012 pmid:35225881 pmcid:PMC8883880 fatcat:da4tm5hivfhstlfqkzkzuwxqje
more »... be the case of a 19-month-old boy who accessed the emergency room initially for a head trauma. The clinical evaluation, however, revealed an unexplained serious respiratory distress needing tracheal intubation. After our evaluation, we hypothesized that the severe respiratory distress determined an altered state of consciousness with following head trauma. The radiological findings raised the suspicion of foreign body aspiration for the presence of an atelectasis of the entire left lung. The computed tomography showed an abrupt interruption of the main bronchus at 12 mm from the hull. The following bronchoscopy identified an almond of 2 cm. We will review the literature to underline the diagnostic issues behind foreign body aspiration in children by highlighting the diagnostic clues that are helpful for emergency clinicians in the management of this condition.
Objectives: This study was aimed at investigating the frequency of the visual phenomenon of palinopsia (visual perseveration) in patients with migraine. Methods: We interviewed 63 patients with migraine with aura (MwA), 137 patients with migraine without aura (MwoA) and 226 sex-age-matched healthy control subjects using an ad hoc structured interview/questionnaire. The interview was divided into four classes of variables for statistical testing. Results: Palinopsia occurred in 19/200 patientsdoi:10.1177/0333102411410083 pmid:21628437 fatcat:2mmertoqfjcqheraf444y6bxrq
more »... .5%); of them 10/63 had MwA and 9/137 MwoA (14.2% vs 6.6%, chi ¼ 9.7, degrees of freedom ¼ 1, p ¼ 0.002). Patients with palinopsia had a significantly lower migraine attack frequency than those without this visual phenomenon (4.3 AE 0.3 vs 14.4 AE 0.2, z ¼ 7.1, p < 0.0001). No healthy control subjects complained of palinopsia according to the structured interview/questionnaire. Discussion: Palinopsia is probably under-diagnosed in patients with migraine. Further investigations are needed to assess whether migraineurs are particularly susceptible to the development of recurrent episodes of visual perseveration.
Creatine plays a crucial role in developing the brain, so much that its genetic deficiency results in mental dysfunction and cognitive impairments. Moreover, creatine supplementation is currently under investigation as a preventive measure to protect the fetus against oxidative stress during difficult pregnancies. Although creatine use is considered safe, posing minimal risk to clinical health, we found an alteration in morpho-functional maturation of neurons when male rats were exposed todoi:10.3390/nu11092014 pmid:31461895 pmcid:PMC6770830 fatcat:i2pl3gaj45de7nn6666y24h27e
more »... ine loads during brain development. In particular, increased excitability and enhanced long-term potentiation (LTP) were observed in the hippocampal pyramidal neurons of weaning pups. Since these effects were observed a long time after creatine treatment had been terminated, long-lasting modifications persisting into adulthood were hypothesized. Such modifications were investigated in the present study using morphological, electrophysiological, and calcium imaging techniques applied to hippocampal Cornu Ammonis 1 (CA1) neurons of adult rats born from dams supplemented with creatine. When compared to age-matched controls, the treated adult offspring were found to retain enhanced neuron excitability and an improved LTP, the best-documented neuronal substrate for memory formation. While translating data from rats to humans does have limitations, our findings suggest that prenatal creatine supplementation could have positive effects on adult cognitive abilities.
Case summary An 11-year-old female spayed domestic shorthair cat was referred with a 2-month history of ptyalism, hyporexia and weight loss. Physical examination revealed reduced body condition score (2/9) and decreased skin turgor. Laboratory abnormalities included mild erythrocytosis, elevated creatine kinase, hypercobalaminaemia and hypofolataemia. CT of the head and abdominal ultrasonography were within normal limits. Gastroesophagoscopy revealed mucosal ulceration and possible stenosis ofdoi:10.1177/2055116919874902 pmid:31555461 pmcid:PMC6753522 fatcat:zx5tbjcmczc4bhyuzwvqyvppam
more »... he distal oesophagus. Thoracic radiographs and iodine oesophagram showed a soft tissue opacity in the caudodorsal thorax compatible with a parietal oesophageal mass causing luminal stenosis or an extra-parietal mass causing ventral displacement and compression of the oesophagus. Pulmonary nodules were observed in the cranial lung lobes. CT of the thorax confirmed the oesophageal origin of the mass and the presence of pulmonary nodules scattered throughout the lung parenchyma. The patient was euthanased given the imaging findings and perceived guarded prognosis. Post-mortem examination revealed multifocal nodular lesions affecting the oesophagus, lungs, kidneys and pancreas. Histopathological examination identified atypical round cells characterised by eosinophilic cytoplasm and pale nuclei with prominent nuclear grooves, compatible with neoplastic histiocytic cells. Immunohistochemistry revealed strong expression for CD18, Iba-1 and vimentin. Transmission electron microscopy demonstrated intracytoplasmic organelles consistent with Birkbeck granules of Langerhans cell origin in lesional histiocytes. These findings were compatible with a diagnosis of disseminated Langerhans cell histiocytosis. Relevance and novel information To our knowledge, this is the first report of disseminated Langerhans cell histiocytosis with oesophageal involvement in a cat.
Myotendinous junctions (MTJs) are anatomical regions specialized in transmission of contractile strength from muscle to tendon and, for this reason, a common site where acute injuries occur during sport activities. In this work we investigated the influence of exercise intensity on MTJ plasticity, as well as on the expression of insulin-like growth factor 1 (IGF-1) and transforming growth factor beta (TGF-β) and their receptors in muscle and tendon. Three groups of rats were analyzed: controldoi:10.1371/journal.pone.0158059 pmid:27337061 pmcid:PMC4918954 fatcat:4rp2u5kdtrbuvi6dhqnwgpiwle
more »... TRL), slow-runner (RUN-S) and fast-runner (RUN-F) trained using a treadmill. Ultrastructural and morphometric analyses of distal MTJs from extensor digitorum longus muscles have been performed. Contractile strength and hypertrophy were investigated by using in vivo tension recordings and muscle cross-sectional area (CSA) analysis, respectively. mRNA levels of PGC-1α, vinculin, IGF-1Ea and TGF-β have been quantified in muscle belly, while IGF-1Ea, TGF-β and their receptors in tendon. Morphometry revealed an increased MTJ complexity and interaction surface between tissues in trained rats according to training intensity. CSA analysis excluded hypertrophy among groups, while muscle strength was found significantly enhanced in exercised rats in comparison to controls. In muscle tissue, we highlighted an increased mRNA expression of PGC-1α and vinculin in both trained conditions and of TGF-β in RUN-F. In tendon, we mainly noted an enhancement of TGF-β mRNA expression only in RUN-F group and a raise of Betaglycan tendon receptor mRNA levels proportional to exercise intensity. In conclusion, MTJ plasticity appears to be related to exercise intensity and molecular analysis suggests a major role played by TGF-β.
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