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Mudra: Choreography in Hands

Sreenath Nair
2013 Body, Space & Technology Journal  
Mudra is a domain of movement, and it is considered to be a prominent mode of communication in dance and the dramatic arts in India. Mudra, primarily, is understood as an inscriptive form that follows the linguistic parameters, expressing symbolic meaning through systematically codified patterns of the dancer's hands. Beyond the level of linguistic symbolism, mudra is kinetic energy. It is essentially a form of movement, and the deliverance of its meaning is embedded in the kinetic modalities
more » ... the hands. A mudra is a 'thing' composed of a number of spatiotemporal properties such as tempo, duration, rhythm, motion trajectories of the hands and eye movements. Mudra is the 'optical mechanism' of the hand, so it sees things, narrates events, interprets their meaning and experiences a range of emotions through movements. Mudra connects the subjective and objective worlds in a performance through kinetic properties of the hands. The body remembers and repeats through the embodied thinking of the gestural practice of the hands.
doi:10.16995/bst.61 fatcat:pbdhunxfizbnngtrbrrnjkv5cq

Functional Imaging of Chemically Active Surfaces with Optical Reporter Microbeads

Punkaj Ahuja, Sumitha Nair, Sreenath Narayan, Miklós Gratzl, Aristides Docoslis
2015 PLoS ONE  
Nair MG. Performed the experiments: PA S. Nair. Analyzed the data: PA S. Narayan. Contributed reagents/materials/analysis tools: PA S. Nair. Wrote the paper: PA MG.  ... 
doi:10.1371/journal.pone.0136970 pmid:26332766 pmcid:PMC4558047 fatcat:ppgwd3jbgfdudklw6e2y44bh5q

Recurrent Cholangiocarcinoma Presenting as Sister Mary Joseph Nodule After Liver Transplantation

Vijayadershan Muppidi, Sreenath Meegada, James D Eaton, Satheesh P Nair, Rajanshu Verma
2020 Cureus  
Hilar cholangiocarcinoma, also known as Klatskin tumor, is the most common type of cholangiocarcinoma. It usually has a lymphatic spread and is rarely associated with an umbilical nodule, also known as Sister Mary Joseph nodule. We report a case of a 53-year-old Caucasian man with hilar cholangiocarcinoma. The patient had an inoperable tumor and was referred to our center for liver transplantation. Post liver transplantation, the patient presented with a recurrence of the carcinoma in the
more » ... cal region. The patient was found to have Sister Mary Joseph nodule. It carries a poor prognosis, and our patient succumbed to the illness in four months. Cholangiocarcinoma carries a poor prognosis. Surgical resection and liver transplantation with neoadjuvant chemoradiation are the preferred treatment strategies. Association of cholangiocarcinoma with umbilical metastasis is rare, and our patient had an even rarer presentation in the form of recurrence with umbilical nodule post-liver transplantation. We want to increase the awareness of the rare presentation, association, and recurrence of hilar cholangiocarcinoma in the form of umbilical nodule post-liver transplantation.
doi:10.7759/cureus.11673 pmid:33262920 pmcid:PMC7689875 fatcat:k3f2amjbkzcalagnybwmsmat4m

PDB ProtVista: A reusable and open-source sequence feature viewer [article]

Mandar Deshpande, Mihaly Varadi, Typhaine Paysan-Lafosse, Sreenath Nair, Damiano Piovesan, Saqib Mir, Aleksandras Gutmanas, Silvio C. E. Tosatto, Sameer Velankar
2022 bioRxiv   pre-print
Using publicly available API endpoints (Nair et al., 2021) , PDB ProtVista can list all the known structures of Calpastatin in the PDB and predicted models from AlphaFold DB and SWISS-MODEL (Tunyasuvunakool  ... 
doi:10.1101/2022.07.22.500790 fatcat:mwrxv4q5bjhglhrzsrrz5lutuy

LV DYSFUNCTION IN COPD PATIENTS AND ITS CORRELATION WITH CLINICAL AND ECG FINDINGS
English

Sreenivasan Sreenath, Santhosh Kumar Thulaseedharan Saraswathy, Rajeevan Sreekumaran Nair
2017 Journal of Evidence Based Medicine and Healthcare  
BACKGROUND Chronic obstructive pulmonary disease is a chronic debilitating disease that is characterised by poorly reversible airflow limitation. COPD includes emphysema, chronic bronchitis and small airway diseases. It is a leading cause of mortality worldwide. The prevalence of COPD increases with age. The lungs and the heart are so closely interrelated organs that diseases of the one results in impaired functioning of the other. A high prevalence of left ventricular dysfunction has been
more » ... ed to exist in patients with COPD and this might result in poor quality of life and exercise intolerance in COPD patients. MATERIALS AND METHODS 64 patients with COPD confirmed by spirometry were examined clinically after taking relevant history. ECG and chest x-ray findings were noted. Echocardiography was done for these patients to assess the left ventricular functioning and degree of pulmonary artery hypertension. RESULTS LV diastolic dysfunction was found in 84% of the patients with COPD in this study. 37.5% had stage I, 46.9% had stage II LV diastolic dysfunction. CONCLUSION In our study, it was found that LV diastolic dysfunction was highly prevalent in patients with moderate-to-severe COPD. LV diastolic dysfunction could be a contributing factor to worsening dyspnoea and exercise intolerance in COPD patients.
doi:10.18410/jebmh/2017/663 fatcat:td3t5xowond3bidlama6ozflgi

Trust and Reputation Mechanisms in Vehicular Ad-Hoc Networks: A Systematic Review

Amit Kumar Tyagi, A. Mohan Krishna, Shaveta Malik, Meghna Manoj Nair, Sreenath Niladhuri
2020 Advances in Science, Technology and Engineering Systems  
An emerging trend has been observed in the Trust and Reputation (T & R) systems in field of decision-making support for a majority of the provisions propagated by the Internet. It is the extreme importance that peers (users) are able to trust each other and rely on them for file sharing and for services. This paper provides the reader an apprehensive and completely true information and details on a large number of the present conceptions, proposals, issues, and the key to those problems in
more » ... s and other fields to enhance the eminence of the data in transportation through a systematized literature review. Trust and reputation have also been discussed gravely in this paper. After the scrutinized analysis of more than 90 articles related to trust in a plethora of fields, extracted from few of the apt scientific sources ((i.e.
doi:10.25046/aj050150 fatcat:x4zp2za2h5h4tgkscherb5xjiy

Facilitative lysosomal transport of bile acids alleviates ER stress in mouse hematopoietic precursors

Avinash K Persaud, Sreenath Nair, Md Fazlur Rahman, Radhika Raj, Brenna Weadick, Debasis Nayak, Craig McElroy, Muruganandan Shanmugam, Sue Knoblaugh, Xiaolin Cheng, Rajgopal Govindarajan
2021 Nature Communications  
Mutations in human equilibrative nucleoside transporter 3 (ENT3) encoded by SLC29A3 results in anemia and erythroid hypoplasia, suggesting that ENT3 may regulate erythropoiesis. Here, we demonstrate that lysosomal ENT3 transport of taurine-conjugated bile acids (TBA) facilitates TBA chemical chaperone function and alleviates endoplasmic reticulum (ER) stress in expanding mouse hematopoietic stem and progenitor cells (HSPCs). Slc29a3-/- HSPCs accumulate less TBA despite elevated levels of TBA in
more » ... Slc29a3-/- mouse plasma and have elevated basal ER stress, reactive oxygen species (ROS), and radiation-induced apoptosis. Reintroduction of ENT3 allows for increased accumulation of TBA into HSPCs, which results in TBA-mediated alleviation of ER stress and erythroid apoptosis. Transplanting TBA-preconditioned HSPCs expressing ENT3 into Slc29a3-/- mice increase bone marrow repopulation capacity and erythroid pool size and prevent early mortalities. Together, these findings suggest a putative role for a facilitative lysosomal transporter in the bile acid regulation of ER stress in mouse HSPCs which may have implications in erythroid biology, the treatment of anemia observed in ENT3-mutated human genetic disorders, and nucleoside analog drug therapy.
doi:10.1038/s41467-021-21451-6 pmid:33623001 fatcat:5tb7m3hruvatfmnddynfsalmma

Estimation and quantitation of β-asarone from Acorus calamus rhizome and its formulations using validated RP-HPLC method

Sunita Shailajan, Sasikumar Menon, Gauri Swar, Dipti Singh, Sreenath Nair
2015 Pharmaceutical Methods  
.: Estimation of β-asarone from Acorus calamus and its formulations Sunita Shailajan 1* , Sasikumar Menon 2 , Gauri Swar 1 , Dipti Singh 1 and Sreenath Nair 2 Herbal Research Lab, Ramnarain Ruia College  ... 
doi:10.5530/phm.2015.6.13 fatcat:yurvgrubjfcspgkklmk7qrvmmm

Adult stem cell deficits drive Slc29a3 disorders in mice

Sreenath Nair, Anne M. Strohecker, Avinash K. Persaud, Bhawana Bissa, Shanmugam Muruganandan, Craig McElroy, Rakesh Pathak, Michelle Williams, Radhika Raj, Amal Kaddoumi, Alex Sparreboom, Aaron M. Beedle (+1 others)
2019 Nature Communications  
Mutations exclusively in equilibrative nucleoside transporter 3 (ENT3), the only intracellular nucleoside transporter within the solute carrier 29 (SLC29) gene family, cause an expanding spectrum of human genetic disorders (e.g., H syndrome, PHID syndrome, and SHML/RDD syndrome). Here, we identify adult stem cell deficits that drive ENT3-related abnormalities in mice. ENT3 deficiency alters hematopoietic and mesenchymal stem cell fates; the former leads to stem cell exhaustion, and the latter
more » ... ads to breaches of mesodermal tissue integrity. The molecular pathogenesis stems from the loss of lysosomal adenosine transport, which impedes autophagy-regulated stem cell differentiation programs via misregulation of the AMPK-mTOR-ULK axis. Furthermore, mass spectrometry-based metabolomics and bioenergetics studies identify defects in fatty acid utilization, and alterations in mitochondrial bioenergetics can additionally propel stem cell deficits. Genetic, pharmacologic and stem cell interventions ameliorate ENT3-disease pathologies and extend the lifespan of ENT3-deficient mice. These findings delineate a primary pathogenic basis for the development of ENT3 spectrum disorders and offer critical mechanistic insights into treating human ENT3-related disorders.
doi:10.1038/s41467-019-10925-3 pmid:31270333 pmcid:PMC6610100 fatcat:k27y5zhwongfzbroqu4rzeysny

CNS Penetration of Cyclophosphamide and Metabolites in Mice Bearing Group 3 Medulloblastoma and Non-Tumor Bearing Mice

Clinton Stewart, Olivia Campagne, Abigail Davis, Bo Zhong, Sreenath Nair, Victoria Haberman, Yogesh T. Patel, Laura Janke, Martine F. Roussel
2019 Journal of Pharmacy & Pharmaceutical Sciences  
PURPOSE: Cyclophosphamide is widely used to treat children with medulloblastoma; however, little is known about its brain penetration. We performed cerebral microdialysis to characterize the brain penetration of cyclophosphamide (130 mg/kg, IP) and its metabolites [4-hydroxy-cyclophosphamide (4OH-CTX) and carboxyethylphosphoramide mustard (CEPM)] in non-tumor bearing mice and mice bearing orthotopic Group 3 medulloblastoma. METHODS: A plasma pharmacokinetic study was performed in
more » ... ng CD1-nude mice, and four cerebral microdialysis studies were performed in non-tumor-bearing (M1 and M3) and tumor-bearing mice (M2 and M4). Plasma samples were collected up to 6-hours post-dose, and extracellular fluid (ECF) samples were collected over 60-minute intervals for 24-hours post-dose. To stabilize and quantify 4OH-CTX, a derivatizing solution was added in blood after collection, and either directly in the microdialysis perfusate (M1 and M2) or in ECF collection tubes (M3 and M4). Plasma/ECF cyclophosphamide and CEPM, and 4OH-CTX concentrations were separately measured using different LC-MS/MS methods. RESULTS: All plasma/ECF concentrations were described using a population-based pharmacokinetic model. Plasma exposures of cyclophosphamide, 4OH-CTX, and CEPM were similar across studies (mean AUC=112.6, 45.6, and 80.8 µmol∙hr/L). Hemorrhage was observed in brain tissue when the derivatizing solution was in perfusate compared with none when in collection tubes, which suggested potential sample contamination in studies M1 and M2. Model-derived unbound ECF to plasma partition coefficients (Kp,uu) were calculated to reflect CNS penetration of the compounds. Lower cyclophosphamide Kp,uu was obtained in tumor-bearing mice versus non-tumor bearing mice (mean 0.15 versus 0.22, p=0.019). No differences in Kp,uu were observed between these groups for 4OH-CTX and CEPM (overall mean 0.10 and 0.07). CONCLUSIONS: Future studies will explore potential mechanisms at the brain-tumor barrier to explain lower cyclophosphamide brain penetration in tumor-bearing mice. These results will be used to further investigate exposure-response relationships in medulloblastoma xenograft models.
doi:10.18433/jpps30608 pmid:31815662 pmcid:PMC7413074 fatcat:whhmg3fztfbm5lhgugszuqo2kq

Exposure-Toxicity Association of Cyclophosphamide and Its Metabolites in Infants and Young Children with Primary Brain Tumors: Implications for Dosing

Olivia Campagne, Bo Zhong, Sreenath Nair, Tong Lin, Jie Huang, Arzu Onar-Thomas, Giles Robinson, Amar Gajjar, Clinton F. Stewart
2019 Clinical Cancer Research  
To characterize the population pharmacokinetics of cyclophosphamide, active 4-hydroxy-cyclophosphamide (4OH-CTX), and inactive carboxyethylphosphoramide mustard (CEPM), and their associations with hematologic toxicities in infants and young children with brain tumors. To use this information to provide cyclophosphamide dosing recommendations in this population. Patients received four cycles of a 1-hour infusion of 1.5 g/m2 cyclophosphamide. Serial samples were collected to measure
more » ... de, 4OH-CTX, and CEPM plasma concentrations. Population pharmacokinetic modeling was performed to identify the patient characteristics influencing drug disposition. Associations between drug exposures and metrics reflecting drug-induced neutropenia, erythropenia, and thrombocytopenia were investigated. A Bayesian approach was developed to predict 4OH-CTX exposure using only cyclophosphamide and CEPM plasma concentrations. Data from 171 patients (0.07-4.9 years) were adequately fitted by a two-compartment (cyclophosphamide) and one-compartment model (metabolites). Young infants (<6 months) exhibited higher mean 4OH-CTX exposure than did young children (138.4 vs. 107.2 μmol/L·h, P < 0.0001). No genotypes exhibited clinically significant influence on drug exposures. Worse toxicity metrics were significantly associated with higher 4OH-CTX exposures. Dosing simulations suggested decreased cyclophosphamide dosage to 1.2 g/m2 for young infants versus 1.5 g/m2 for children to attain similar 4OH-CTX exposure. Bayesian-modeled 4OH-CTX exposure predictions were precise (mean absolute prediction error 14.8% ± 4.2%) and had low bias (mean prediction error 4.9% ± 5.1%). A 4OH-CTX exposure-toxicity association was established, and a decreased cyclophosphamide dosage for young infants was suggested to reduce toxicity in this population. Bayesian modeling to predict 4OH-CTX exposure may reduce clinical processing-related costs and provide insights into further exposure-response associations.
doi:10.1158/1078-0432.ccr-19-2685 pmid:31796512 pmcid:PMC7124978 fatcat:v3itwxpkgnfgri3vp5dyrcgpre

Investigating the Central Nervous System Disposition of Actinomycin D: Implementation and Evaluation of Cerebral Microdialysis and Brain Tissue Measurements Supported by UPLC-MS/MS Quantification

Julia Benzel, Gzona Bajraktari-Sylejmani, Philipp Uhl, Abigail Davis, Sreenath Nair, Stefan M. Pfister, Walter E. Haefeli, Johanna Weiss, Jürgen Burhenne, Kristian W. Pajtler, Max Sauter
2021 Pharmaceutics  
Actinomycin D is a potent cytotoxic drug against pediatric (and other) tumors that is thought to barely cross the blood–brain barrier. To evaluate its potential applicability for the treatment of patients with central nervous system (CNS) tumors, we established a cerebral microdialysis model in freely moving mice and investigated its CNS disposition by quantifying actinomycin D in cerebral microdialysate, brain tissue homogenate, and plasma. For this purpose, we developed and validated an
more » ... erformance liquid chromatography–tandem mass spectrometry assay suitable for ultra-sensitive quantification of actinomycin D in the pertinent biological matrices in micro-samples of only 20 µL, with a lower limit of quantification of 0.05 ng/mL. In parallel, we confirmed actinomycin D as a substrate of P-glycoprotein (P-gp) in in vitro experiments. Two hours after intravenous administration of 0.5 mg/kg, actinomycin D reached total brain tissue concentrations of 4.1 ± 0.7 ng/g corresponding to a brain-to-plasma ratio of 0.18 ± 0.03, while it was not detectable in intracerebral microdialysate. This tissue concentration exceeds the concentrations of actinomycin D that have been shown to be effective in in vitro experiments. Elimination of the drug from brain tissue was substantially slower than from plasma, as shown in a brain-to-plasma ratio of approximately 0.53 after 22 h. Because actinomycin D reached potentially effective concentrations in brain tissue in our experiments, the drug should be further investigated as a therapeutic agent in potentially susceptible CNS malignancies, such as ependymoma.
doi:10.3390/pharmaceutics13091498 pmid:34575574 fatcat:a46aekvmdre3piyp7jo65p6sm4

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

Mihaly Varadi, Stephen Anyango, Mandar Deshpande, Sreenath Nair, Cindy Natassia, Galabina Yordanova, David Yuan, Oana Stroe, Gemma Wood, Agata Laydon, Augustin Žídek, Tim Green (+15 others)
2021 Nucleic Acids Research  
The AlphaFold Protein Structure Database (AlphaFold DB, https://alphafold.ebi.ac.uk) is an openly accessible, extensive database of high-accuracy protein-structure predictions. Powered by AlphaFold v2.0 of DeepMind, it has enabled an unprecedented expansion of the structural coverage of the known protein-sequence space. AlphaFold DB provides programmatic access to and interactive visualization of predicted atomic coordinates, per-residue and pairwise model-confidence estimates and predicted
more » ... ned errors. The initial release of AlphaFold DB contains over 360,000 predicted structures across 21 model-organism proteomes, which will soon be expanded to cover most of the (over 100 million) representative sequences from the UniRef90 data set.
doi:10.1093/nar/gkab1061 pmid:34791371 pmcid:PMC8728224 fatcat:m3lwyaoecnbxnhegqu4arkqhs4

3D-Beacons: Decreasing the gap between protein sequences and structures through a federated network of protein structure data resources [article]

Mihaly Varadi, Sreenath S Nair, Ian Sillitoe, Gerardo Tauriello, Stephen Anyango, Stefan Bienert, Clemente Borges, Mandar Deshpande, Tim Green, Andras Hatos, Tamas Hegedus, Maarten L Hekkelman (+20 others)
2022 bioRxiv   pre-print
While scientists can often infer the biological function of proteins from their 3-dimensional quaternary structures, the gap between known protein sequences and their experimentally determined structures keeps increasing. A potential solution to this problem is presented by ever more sophisticated theoretical protein modelling approaches. While often powerful on their own, most methods have strengths and weaknesses. Therefore, it benefits researchers to examine models from various data
more » ... and perform comparative analysis to identify what models best address their specific use cases. To make data from a large array of model providers more easily accessible to the broader scientific community, we established 3D-Beacons, a collaborative initiative to create a federated network with unified data access mechanisms. 3D-Beacons allows researchers to collate coordinate files and metadata for experimentally determined and theoretical protein models from state-of-the-art and specialist model providers and the Protein Data Bank.
doi:10.1101/2022.08.01.501973 fatcat:7lij36comrfklicvhytg6akriy

Contributors

2016 Translation Today  
Saratchndran Nair V. Saratchndran Nair is currently working as the Project Director, National Translation Mission, CIIL, Mysore.  ...  Sreenath V. S Sreenath V.S is a doctoral student of English in the department of Humanities and Social Sciences, Indian Institute of Technology Kanpur.  ... 
doi:10.46623/tt/2016.10.1.cn fatcat:virtbpg2nnemthv32hxtldgr5u
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