Filters








53 Hits in 2.2 sec

Gene Expression in Chronic Fatigue Syndrome [chapter]

Ekua W., Kevin J., Gunn M., Donald R., Sonya Marshall-Gradisnik
2012 An International Perspective on the Future of Research in Chronic Fatigue Syndrome  
Bahr, J., Kloting, N., Kloting, I. and Follak, N. (2011 References Akakura, N., Kobayashi, M., Horiuchi, I., Suzuki, A., Wang, J., Chen, J., Niizeki, H., Kawamura, K., Hosokawa, M. and Asaka, M. (  ...  CD2 is regulated by CD2BP2 (the CD2 binding protein 2) which increases binding specificity of the cytoplasmic domain of the T cell adhesion molecule CD2 and localizes it to the cell m e m b r a n e a n  ... 
doi:10.5772/30342 fatcat:zpkq2cji4vgbzehysy6zgj7ocy

Immune and hemorheological changes in Chronic Fatigue Syndrome

Ekua W Brenu, Donald R Staines, Oguz K Baskurt, Kevin J Ashton, Sandra B Ramos, Rhys M Christy, Sonya M Marshall-Gradisnik
2010 Journal of Translational Medicine  
This method generates two distinct measures of erythrocyte aggregation at stasis (M 0 ) and at a low shear (M 1 ) after a shear rate of 600 s -1 .  ...  Peripheral blood samples from CFS patients (black; n = 10) and healthy controls (white; n = 10) assessed on measures of aggregation at stasis (M 0 ) and at low shear rate (M 1 ).  ... 
doi:10.1186/1479-5876-8-1 pmid:20064266 pmcid:PMC2829521 fatcat:4ou45kc4pnfldns22uvve6ly7u

T Helper Cell Cytokine Profiles After Endurance Exercise

Michael W. Kakanis, Jonathan Peake, Ekua W. Brenu, Michael Simmonds, Bon Gray, Sonya M. Marshall-Gradisnik
2014 Journal of Interferon and Cytokine Research  
Ten highly-trained road cyclists (mean ± SEM: age 24.2 ± 1.7 4 yr; height 1.82 ± 0.02 m; body mass 73.8 ± 2.0 kg; peak oxygen uptake 65.9 ± 2.3 ml.kg -5 1 .min -1 ) performed 2 h of cycling exercise at  ...  Briefly, 6 plasma samples were treated with 1 M HCl at room temperature to displace cortisol and 7 cortisone from binding proteins.  ...  MATERIALS AND METHODS 24 Subjects 25 Ten well-trained male cyclists volunteered to participate in the present study (mean ± SEM: 26 age 24.2 ± 1.7 yr; height 1.82 ± 0.02 m; body mass 73.8 ± 2.0 kg; peak  ... 
doi:10.1089/jir.2013.0031 pmid:24673178 fatcat:dteahiuftnb27kw2zx34vbp4oi

High-Throughput Sequencing of Plasma MicroRNA in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Ekua W. Brenu, Kevin J. Ashton, Jana Batovska, Donald R. Staines, Sonya M. Marshall-Gradisnik, Sue Cotterill
2014 PLoS ONE  
unique reads mapped to mature miRNAs, other microRNAs (ambiguous, star and hairpin), other non-coding RNAs and unmapped. doi:10.1371/journal.pone.0102783.g001 Figure 2 . 2 Expression stability values (M)  ... 
doi:10.1371/journal.pone.0102783 pmid:25238588 pmcid:PMC4169517 fatcat:caytx66rknbhbawlf4lgnj6him

Brain function characteristics of chronic fatigue syndrome: A task fMRI study

Zack Y. Shan, Kevin Finegan, Sandeep Bhuta, Timothy Ireland, Donald R. Staines, Sonya M. Marshall-Gradisnik, Leighton R. Barnden
2018 NeuroImage: Clinical  
, …, x i+m−1 }, that are similar within a tolerance r, remain similar when the segments were extended to include the next data point (Eq. (1)). = − + SampEn C C ln , m m 1 (1) where C m is the count of  ...  (2) , ≠ = − d X i X j i j max x x [ ( ), ( )] ( ) (| |). m m i i j  (2) In this study, the length m was set to 3 and the tolerance r was set to 0.2 times of the standard deviation of the full time  ... 
doi:10.1016/j.nicl.2018.04.025 pmid:30035022 pmcid:PMC6051500 fatcat:m3hdivjyibcntjwel3em4zsnia

Natural killer cell cytotoxic activity: measurement of the apoptotic inducing mechanisms

Teilah K. Huth, Ekua W. Brenu, Kevin J. Ashton, Don R. Staines, Sonya M. Marshall-Gradisnik
2013 Clinical and Experimental Medical Sciences  
Natural Killer cell cytotoxic activity is vital for the clearance of viral and malignantly transformed cells. The aim of this study was to investigate the apoptotic inducing mechanisms of NK cells to propose an additional measurement for NK cell effector function. 19 healthy controls (age=31±7.2 years) participated in this study. Flow cytometric protocols assessed NK cell cytotoxic activity against tumour K562 cells, lytic proteins, degranulation and interferon gamma production. Perforin
more » ... was significantly correlated with cytotoxic activity (r=-0.46, p<0.05) and degranulation (r=-0.60, p<0.05). These results suggest that perforin may be an additional measure for NK cell cytotoxic effector function.
doi:10.12988/cems.2013.13031 fatcat:rqt6mvgb45hvbfqot2dddbyar4

Enhanced Gene Expression Following Vaccination in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Ekua W. Brenu, Gunn M. Atkinson, Mieke L. van Driel, Sanne Kreijkamp-Kaspers, Don R. Staines, Sonya M. Marshall-Gradisnik
2013 International journal of clinical medicine  
M-values greater than 1 were rejected while an M-value less than 1 for a particular gene was considered the most stable.  ...  Figure 1 .Figure 2 . 12 Determination of the appropriate reference gene for gene expression based on M-values (average expression).  ... 
doi:10.4236/ijcm.2013.43029 fatcat:k7aywhberbdzfb26vb6df5nwai

Dysregulation of Protein Kinase Gene Expression in NK Cells from Chronic Fatigue Syndrome/Myalgic Encephalomyelitis Patients

Anu Chacko, Donald R. Staines, Samantha C. Johnston, Sonya M. Marshall-Gradisnik
2016 Gene Regulation and Systems Biology  
isolation ( Supplementary Fig. 1 ), the NK cell purity was also assessed on the LSR-Fortessa X-20 flow cytometer (BD Biosciences) after labeling with CD3 and CD56, as previously described in the study by Marshall-Gradisnik  ... 
doi:10.4137/grsb.s40036 pmid:27594784 pmcid:PMC5003121 fatcat:3dfmk76jbjg3nkermwgt4wje4i

Medial prefrontal cortex deficits correlate with unrefreshing sleep in patients with chronic fatigue syndrome

Zack Y. Shan, Richard Kwiatek, Richard Burnet, Peter Del Fante, Donald R. Staines, Sonya M. Marshall-Gradisnik, Leighton R. Barnden
2017 NMR in Biomedicine  
, PSQI & anxiety were Table 1 . 1 Means of symptom scores and global GM and WM volumes of CFS and NC groups § : GM: grey matter, WM: white matter, CFS: chronic fatigue syndrome, NC: normal control, M:  ... 
doi:10.1002/nbm.3757 pmid:28661067 fatcat:nsrtivi5hzhs7daogspmdy3sl4

Progressive brain changes in patients with chronic fatigue syndrome: A longitudinal MRI study

Zack Y. Shan, Richard Kwiatek, Richard Burnet, Peter Del Fante, Donald R. Staines, Sonya M. Marshall-Gradisnik, Leighton R. Barnden
2016 Journal of Magnetic Resonance Imaging  
TABLE 3 . 3 Means of Clinical Measures and Global GM and WM Volumes of Pooled CFS and NC Groups Characteristics M CSF 6 SD M NC 6 SD P GM 659 6 72.6 ml 693.5 6 83.5 ml 0.14 WM 492.7 6 58.9 ml  ...  < 0.0062 Neuro SS 20.35 6 6.72 35.5 6 3.41 < 0.0062 Somatic SS 32.88 6 8.51 55.52 6 3.06 < 0.0062 GM 5 gray matter; WM 5 white matter; CFS 5 chronic fatigue syndrome; NC 5 normal control; M  ... 
doi:10.1002/jmri.25283 pmid:27123773 pmcid:PMC5111735 fatcat:ks5bjgbvmvg4xo3tc2sxbruo7e

Role of adaptive and innate immune cells in chronic fatigue syndrome/myalgic encephalomyelitis

Ekua Weba Brenu, Teilah K. Huth, Sharni L. Hardcastle, Kirsty Fuller, Manprit Kaur, Samantha Johnston, Sandra B. Ramos, Don R. Staines, Sonya M. Marshall-Gradisnik
2013 International Immunology  
Perturbations in immune processes are a hallmark of a number of autoimmune and inflammatory disorders. Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is an inflammatory disorder with possible autoimmune correlates, characterized by reduced NK cell activity, elevations in regulatory T cells (Tregs) and dysregulation in cytokine levels. The purpose of this article is to examine innate and adaptive immune cell phenotypes and functional characteristics that have not been previously
more » ... ined in CFS/ME patients. Thirty patients with CFS/ME and 25 non-fatigued controls were recruited for this study. Whole blood samples were collected from all participants for the assessment of cell phenotypes, functional properties, receptors, adhesion molecules, antigens and intracellular proteins using flow cytometric protocols. The cells investigated included NK cells, dendritic cells, neutrophils, B cells, T cells, γδT cells and Tregs. Significant changes were observed in B-cell subsets, Tregs, CD4 + CD73 + CD39 + T cells, cytotoxic activity, granzyme B, neutrophil antigens, TNF-α and IFN-γ in the CFS/ME patients in comparison with the non-fatigued controls. Alterations in B cells, Tregs, NK cells and neutrophils suggest significant impairments in immune regulation in CFS/ME and these may have similarities to a number of autoimmune disorders.
doi:10.1093/intimm/dxt068 pmid:24343819 fatcat:k5eqmyhfsbfzzoruyu22pzklqm

Immunological abnormalities as potential biomarkers in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Ekua W Brenu, Mieke L van Driel, Don R Staines, Kevin J Ashton, Sandra B Ramos, James Keane, Nancy G Klimas, Sonya M Marshall-Gradisnik
2011 Journal of Translational Medicine  
Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is characterised by severe prolonged fatigue, and decreases in cognition and other physiological functions, resulting in severe loss of quality of life, difficult clinical management and high costs to the health care system. To date there is no proven pathomechanism to satisfactorily explain this disorder. Studies have identified abnormalities in immune function but these data are inconsistent. We investigated the profile of markers of
more » ... immune function (including novel markers) in CFS/ME patients. Methods: We included 95 CFS/ME patients and 50 healthy controls. All participants were assessed on natural killer (NK) and CD8 + T cell cytotoxic activities, Th1 and Th2 cytokine profile of CD4 + T cells, expression of vasoactive intestinal peptide receptor 2 (VPACR2), levels of NK phenotypes (CD56 bright and CD56 dim ) and regulatory T cells expressing FoxP3 transcription factor. Results: Compared to healthy individuals, CFS/ME patients displayed significant increases in IL-10, IFN-γ, TNF-α, CD4 + CD25 + T cells, FoxP3 and VPACR2 expression. Cytotoxic activity of NK and CD8 + T cells and NK phenotypes, in particular the CD56 bright NK cells were significantly decreased in CFS/ME patients. Additionally granzyme A and granzyme K expression were reduced while expression levels of perforin were significantly increased in the CFS/ME population relative to the control population. These data suggest significant dysregulation of the immune system in CFS/ME patients. Conclusions: Our study found immunological abnormalities which may serve as biomarkers in CFS/ME patients with potential for an application as a diagnostic tool.
doi:10.1186/1479-5876-9-81 pmid:21619669 pmcid:PMC3120691 fatcat:dpzrpaf4knegbojjyutjwn2vtu

Effects of estrogen on the mechanical behavior of the human Achilles tendon in vivo

Adam L. Bryant, Ross A. Clark, Simon Bartold, Aron Murphy, Kim L. Bennell, Erik Hohmann, Sonya Marshall-Gradisnik, Craig Payne, Kay M. Crossley
2008 Journal of applied physiology  
and running history of the non-MOCP and MOCP users together with P values derived from each t-test contrast Non-MOCP MOCP Dependent Variables Users Users P Value Age, yr 31.9Ϯ7.3 28.0Ϯ4.2 0.051 Height, m  ... 
doi:10.1152/japplphysiol.01281.2007 pmid:18566188 fatcat:52okbs55l5aaddwklz2vtfox5m

Varied Walking Dose In Women With Type 2 Diabetes: Heart Rate Variability And Hemorheology Responses

Michael J. Simmonds, Clare L. Minahan, Kevin R. Serre, Gregory C. Gass, Sonya M. Marshall-Gradisnik, Luke J. Haseler, Surendran Sabapathy
2011 Medicine & Science in Sports & Exercise  
doi:10.1249/01.mss.0000402131.51736.79 fatcat:c2o6nfdfozbrjbtkshdzoijbou

Examination of Single Nucleotide Polymorphisms (SNPs) in Transient Receptor Potential (TRP) Ion Channels in Chronic Fatigue Syndrome Patients

Sonya M. Marshall-Gradisnik, Peter Smith, Ekua W. Brenu, Bernd Nilius, Sandra B. Ramos, Donald R. Staines
2015 Immunology and Immunogenetics Insights  
BACKGROUND: The transient receptor potential (TRP) superfamily in humans comprises 27 cation channels with permeability to monovalent and divalent cations. These channels are widely expressed within humans on cells and tissues and have significant sensory and regulatory roles on most physiological functions. Chronic fatigue syndrome (CFS) is an unexplained disorder with multiple physiological impairments. OBJECTIVES: The purpose of this study was to determine the role of TRPs in CFS. METHODS:
more » ... e study comprised 115 CFS patients (age = 48.68 ± 1.06 years) and 90 nonfatigued controls (age = 46.48 ± 1.22 years). CFS patients were defined according to the 1994 Center for Disease Prevention and Control criteria for CFS. A total of 240 single nucleotide polymorphisms (SNPs) for 21 mammalian TRP ion channel genes (TRPA1, and TRPV6) were examined via the Agena Biosciences iPLEX Gold assay. Statistical analysis was performed using the PLINK analysis software. RESULTS: Thirteen SNPs were significantly associated with CFS patients compared with the controls. Nine of these SNPs were associated with TRPM3 (rs12682832; P  0.003, rs11142508; P  0.004, rs1160742; P  0.08, rs4454352; P  0.013, rs1328153; P  0.013, rs3763619; P  0.014, rs7865858; P  0.021, rs1504401; P  0041, rs10115622; P  0.050), while the remainder were associated with TRPA1 (rs2383844; P  0.040, rs4738202; P  0.018) and TRPC4 (rs6650469; P  0.016, rs655207; P  0.018). CONCLUSION: The data from this pilot study suggest an association between TRP ion channels, predominantly TRPM3 and CFS. This and other TRPs identified may contribute to the etiology and pathomechanism of CFS.
doi:10.4137/iii.s25147 fatcat:oddoeuecjbddpnrff2nayf2ryi
« Previous Showing results 1 — 15 out of 53 results