A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL.
The file type is application/pdf.
Filters
High-throughput profiling of tissue and tissue model microarrays: Combined transmitted light and 3-color fluorescence digital pathology
2011
Journal of Pathology Informatics
Preserved Fulltext
Here we describe the use of the Hamamatsu NanoZoomer Digital Pathology slide scanner with HCImage software for combined brightfield and multiplexed fluorescence biomarker analysis and highlight its applications ...
There is also a need for tissue-based multivariate biomarker assays to improve the accuracy of diagnostic, prognostic, and predictive testing. ...
Higher multiplexing can be achieved by staining and imaging biomarkers in serial sections using the NanoZoomer. ...
doi:10.4103/2153-3539.89849
pmid:22200032
pmcid:PMC3237061
fatcat:bnv3jjsr3zdk7ayletgcjvfoeq
Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic
2014
Laboratory Investigation
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL.
The file type is application/pdf.
Here, we revisit the reporters and methods used for single-and multiplexed in situ visualization of protein and RNA. ...
Then we examine methods for the use of quantitative platforms for in situ measurement of protein and mRNA levels. ...
in a single sample (ie, target multiplexing) provides information on tissue distribution, marker colocalization, and synchronous-level quantitation. ...
doi:10.1038/labinvest.2014.157
pmid:25502176
pmcid:PMC4383674
fatcat:igvzzwxugjhevabejtgszd6gue
Novel Platforms of Multiplexed Immunofluorescence for Study of Paraffin Tumor Tissues
2017
Journal of Cancer Treatment and Diagnosis
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
These new technologies can be valuable for tumor examination in formalin-fixed paraffin-embedded (FFPE) specimens, and for improved new treatment discoveries and translational cancer studies. ...
Although, most of the old platforms are not more used after the powerful multiplex IHC methods are continue growing, the basis of these old methodologies have helped to improve the new technologies. ...
Perkin Elmer developed the Opal ™ workflow ( Figure 2B) , which allows simultaneous staining of multiple biomarkers within a single paraffin tissue section. ...
doi:10.29245/2578-2967/2018/1.1122
fatcat:axoua2apmrb6tcaptqgg5u4dmy
Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections
2019
Microsystems & Nanoengineering
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
As proof-of-concept analyses, we identified coexpression and colocalization patterns of biomarkers to classify the immune cells and their activation status. ...
Here, we present a quantitative method to identify ten different immuno-markers in the same tumor cut section, thereby saving precious samples and enabling correlative analysis on several cell families ...
This work was supported by the Commission for Technology and Innovation (CTI project no. 18257.2 PFLS-LS). ...
doi:10.1038/s41378-019-0104-z
pmid:31700674
pmcid:PMC6831597
fatcat:26akvgeirrd7xm7yjjzob4gpba
High-Throughput Single Extracellular Vesicle Profiling
[chapter]
2021
Secretomics [Working Title]
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL.
The file type is application/pdf.
In this chapter, we will discuss advanced strategies for studying single EVs, including single EV imaging systems, flow cytometers, nano-sensing technologies and single EV barcoding assay. ...
Besides the investigation of EV biology, single EV analysis technologies are promising approach for liquid biopsy, which relies on the detection of biomarker EVs readily available in body fluids but in ...
The colocalization of multiple biomarkers could be simultaneously recorded via multi-color TIR imaging. ...
doi:10.5772/intechopen.97544
fatcat:7ioe3zroqfgjrffgpiw5vdyeqy
Comparison of Multiplexed Immunofluorescence Imaging to Chromogenic Immunohistochemistry of Skin Biomarkers in Response to Monkeypox Virus Infection
2020
Viruses
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
on serial sections and compared to MxIF automated single cell quantification of the same markers that were multiplexed on a single tissue section. ...
multiplexed tissue immunofluorescence (MxIF) method with single cell-level quantitation termed Cell DIVETM. ...
the US Department of Health and Human Services or of the institutions and companies affiliated with the authors. ...
doi:10.3390/v12080787
pmid:32717786
fatcat:4jetz2orwjdcfkimjs5tdzotim
Molecular profiling of single cancer cells and clinical tissue specimens with semiconductor quantum dots
2006
International Journal of Nanomedicine
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Semiconductor quantum dots (QDs) are a new class of fl uorescent labels with broad applications in biomedical imaging, disease diagnostics, and molecular and cell biology. ...
These improved QDs have opened new possibilities for real-time imaging and tracking of molecular targets in living cells, for multiplexed analysis of biomolecular markers in clinical tissue specimens, ...
The work was supported in part by NIH grants (P20 GM072069, R01 CA108468-01, and U54CA119338) and the Georgia Cancer Coalition Distinguished Cancer Scholars Program (to S.N.). ...
doi:10.2147/nano.2006.1.4.473
pmid:17722280
pmcid:PMC2676641
fatcat:kc45u6mmkbbjxn6erahleqoeyy
High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations
2020
Science Advances
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
Concurrent detection of multiple biomarkers at a single-EV level would greatly improve prognosis and/or diagnosis and understanding of EV phenotypes, biogenesis, and functions. ...
Here, we introduced a High-throughput Nano-bio Chip Integrated System for Liquid Biopsy (HNCIB) system for simultaneous detection of proteins and mRNA/miRNA in a single EV. ...
Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. ...
doi:10.1126/sciadv.abc1204
pmid:33219024
pmcid:PMC7679165
fatcat:yqay7o5tvfekpftwmevblwdhgi
Immunocapture strategies in translational proteomics
2015
Espert Review of Proteomics
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is application/pdf.
Acknowledgment The authors would like to thank Devin Sullivan (Cellprofiling -Subcellular Human Protein Atlas; SciLifeLab, School of Biotechnology, KTH -Royal Institute of Technology, Sweden) for the great ...
Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the ...
For example, the xMAP® bead array technology (Luminex Corp., Austin, TX, USA) currently offers up to 500 parallel measurements in a single run. ...
doi:10.1586/14789450.2016.1111141
pmid:26558424
pmcid:PMC4732419
fatcat:fybbxk7bgvdxxa3h33r7by77pi
Covalently deposited dyes: a new chromogen paradigm that facilitates analysis of multiple biomarkers in situ
2016
Laboratory Investigation
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
Multiplexed analysis of multiple biomarkers in a tissue sample requires use of reporter dyes with specific spectral properties that enable discrimination of signals. ...
It is anticipated that this new paradigm will enable generation of a wide variety of new chromogens, useful for both research and clinical biomarker analysis that will benefit clinicians and patients. ...
As a result there is a greater demand for multiplexing technologies that enable analysis of multiple biomarkers on a single tissue specimen. ...
doi:10.1038/labinvest.2016.115
pmid:27869794
fatcat:saqhnbo4mze4de7gfbmk6csmfm
Development of a quantitative pharmacodynamic assay for apoptosis in fixed tumor tissue and its application in distinguishing cytotoxic drug−induced DNA double strand breaks from DNA double strand breaks associated with apoptosis
2018
OncoTarget
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf.
Clinical feasibility was established using formalinfixed, paraffin-embedded biopsies from a canine cancer clinical trial; γH2AX/CC3 colocalization analysis revealed apoptosis induction by two novel indenoisoquinoline ...
Here, we describe an immunofluorescence assay that distinguishes between apoptosis and drug-induced DSBs by measuring coexpression of γH2AX and membrane blebbing− associated CC3 to indicate apoptosis, ...
ACKNOWLEDGMENTS We thank Sandy Eldridge for pathology review and the Comparative Oncology Trials Consortium (COTC) institution veterinarians who enrolled patients and/or served on the Data and Safety Monitoring ...
doi:10.18632/oncotarget.24936
pmid:29682208
pmcid:PMC5908309
fatcat:n3op7m6n3fertpmyvjproht66y
Multifunctional quantum dots for personalized medicine
2009
Nano Today
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
Quantum dots (QDs), semiconductor nanocrystals with unique photo-physical properties, represent a novel class of fluorescence probes to address many of the needs of personalized medicine. ...
However, a number of diseases, such as cancer, immunodeficiencies, and neurological disorders, still evade conventional diagnostic and therapeutic approaches. ...
Acknowledgments This work is support in part by the National Science Foundation for a Faculty Early Career Development award (CAREER 0645080), and the National Institute of Health (R01 CA131797). ...
doi:10.1016/j.nantod.2009.07.004
pmid:20161004
pmcid:PMC2757762
fatcat:pyamnk23pvdglkuo32oheu5lny
CdSe quantum dots evaluation in primary cellular models or tissues derived from patients
2020
Nanomedicine: Nanotechnology, Biology and Medicine
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL.
The file type is application/pdf.
QD-based multiplexed imaging has been proved to overcome most of the major drawbacks of conventional techniques, exhibiting higher sensitivity, reliability, accuracy and simultaneous labelling of key biomarkers ...
Here we review and discuss the studies of these nanoparticles in patient derived cellular samples or tissues, including cellular models from iPSCs from patients, biopsied and post-mortem tissue. ...
the multiplexed staining with conventional single QD labeling, IHC and WB. 59 In a subsequent study, the mQDL enabled a single cell level analysis detecting 4 biomarkers, reducing sample variability ...
doi:10.1016/j.nano.2020.102299
pmid:32931928
fatcat:7xhi3udrmze75cjz7oivb6oijm
Dissecting Tumor-Immune Microenvironment in Breast Cancer at a Spatial and Multiplex Resolution
2022
Cancers
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2022; you can also visit the original URL.
The file type is application/pdf.
Towards improved understanding of the complex interactions in TIME, several emerging multiplex in situ methods are being developed and gaining much attention for protein detection. ...
Surrogates for antitumor immune response have been explored as predictive biomarkers to immunotherapy, though with several limitations. ...
Towards this end, novel multispectral and spatial technologies should be used. ...
doi:10.3390/cancers14081999
pmid:35454904
pmcid:PMC9026731
fatcat:den2rusn2jgbhdmsixnlj7i4lu
High-dimensional immune-profiling in cancer: implications for immunotherapy
2020
Journal for ImmunoTherapy of Cancer
Preserved Fulltext
A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
Conventional technologies used for this purpose were limited by the depth and dimensionality of the data. ...
We highlight herein some of the high-dimensional technologies currently employed in immune profiling in cancer and summarize the insights and potential benefits they could bring in designing better cancer ...
, unbiased, multiplex profiling of both the proteome and transcriptome of a single cell. 58 Protein epitopes are detected via oligonucleotide-conjugated antibodies (similar to Abseq). ...
doi:10.1136/jitc-2019-000363
pmid:32034066
pmcid:PMC7057482
fatcat:v7bf2vo325eerhyr2hzmhpbkei
« Previous
Showing results 1 — 15 out of 609 results