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Genome Mapping by Fluorescent Fingerprinting
1997
Genome Research
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The construction of sequence-ready maps of overlapping genomic clones is central to large-scale genome sequencing. We have implemented a method for fluorescent fingerprinting of bacterial clones to assemble contig maps. The method utilizes three spectrally distinct fluorescently tagged dideoxy ATPs to specifically label the HindIII termini in HindIII and Sau3AI restriction digests of clones that are multiplexed prior to electrophoresis and data collection. There is excellent reproducibility of
doi:10.1101/gr.7.12.1162
pmid:9414321
pmcid:PMC310676
fatcat:vpyfvunu7zh6bkbe6klbpwirdi
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... aw data, improved resolution of large fragments, and concordance between the results obtained using this and the equivalent radioactive protocol. This method also allows detection of smaller overlaps between clones when compared to the analysis of restriction digests on nondenaturing agarose gels.
"Storms of crustal stress" and AE earthquake precursors
2010
Natural Hazards and Earth System Sciences
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et al., 2005 Gregori et al., , 2007 Paparo et al., 2006; Poscolieri et al., 2006a Poscolieri et al., , 2006b . 328 G. ...
However it is a SOC phenomenon, 326 G. P. Gregori et al.: "Storms of crustal stress" and AE earthquake precursors characterized by some irregular superposition of overlapping "crustal substorms". ...
doi:10.5194/nhess-10-319-2010
fatcat:sybwfnmra5dbzp35mfprksyu4y
THE ECONOMICS OF EXOGAMOUS MARRIAGE IN SMALL-SCALE SOCIETIES
2016
Economic Inquiry
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The Social Sciences and Humanities Research Council of Canada and the Human Evolutionary Studies Program at Simon Fraser University provided financial support. All opinions are those of the authors. ...
A household allocation determines the labor allocation as follows: (3) L 1 = 2(h 1 g + h 12 g + h 1 b + h 12 b ) (total labor at site 1) L 2 = 2(h 2 g + h 21 g + h 2 b + h 21 b ) (total labor at site 2 ...
) A household allocation also determines the level of exogamy: (4) m = h 12 g + h 12 b + h 21 g + h 21 b (total mobile matches) (n-m)/2 = h 1 g + h 1 b (total local matches at site 1) (n-m)/2 = h 2 g + ...
doi:10.1111/ecin.12321
fatcat:sbippv7on5hmrg6mh63ww56n2a
Cytokine production associated with smallpox vaccine responses
2014
Immunotherapy
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The effect of the other cytokines included in the decision tree (G-CSF, eotaxin and TIMP-2) on the presfuture science group Review Simon, Salk, Ovsyannikova, Kennedy & Poland ence of AEs post smallpox ...
future science group
Review Simon, Salk, Ovsyannikova, Kennedy & Poland
ity [23,49,50]. ...
doi:10.2217/imt.14.72
pmid:25428648
pmcid:PMC4263415
fatcat:fec7ujzma5eifomiukcaaowtjy
CathKIT: improving quality in the cardiac catheterization laboratory
2004
Journal of the American College of Cardiology
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Chazal, MD, FACC, FSCAI, and Gregory J. Dehmer, MD, FACC, FSCAI, began the development process in late 2001. ...
doi:10.1016/j.jacc.2004.01.014
pmid:14998634
fatcat:xzqbkpu2pjhfze2quot3hu7aqi
Digoxin Targets Central Carbon Metabolism and Remodels the Tumor Microenvironment
[article]
2020
bioRxiv
pre-print
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of mATP1a1 (EX-Mm01329-Lv105, GeneCopoeia) or GFP control (EX-EGFP-Lv105, GeneCopoeia) plasmid, 10 μg of PsPAX2 packaging vector (no. 12260, Addgene), and 5 μg of PMD2.G envelopeexpressing plasmids (no ...
HCT-116 cells were plated in 10-cm plates, and when they reached 30-50% confluency, virus-containing medium (1:1 with fresh RPMI 1640 medium) was added to the plates along with polybrene (4 μg/μl). ...
doi:10.1101/2020.03.31.018739
fatcat:agwrxacdn5beph52o5ksgbspri
Adjustment to amputation and interest in upper limb transplantation
2019
SAGE Open Medicine
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Upper limb transplantation provides a new restorative option for individuals with amputations. As true for most operations, patient selection is critical to optimizing transplantation outcomes. To improve on the patient selection process, we used qualitative methods to better understand the issues regarding upper extremity loss as well as upper limb transplantation from the amputee point of view. Individuals with upper limb amputations (age range = 24-73 years) discussed their adjustment
doi:10.1177/2050312119858248
pmid:31217973
pmcid:PMC6563387
fatcat:two7aojgmzajfgkhd5hc4eitzy
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... ng amputation and their interest toward transplantation in either a focus group (n = 5) or semi-structured interview (n = 17). Transcripts were coded by theme and summarized. Participants described a year-long process typified by adjustment to a new role as an amputee, both psychosocially and functionally. We found that the extent of adjustment was inversely related to an interest in transplantation. These findings could explain the difficulty in identifying "ideal" candidates for upper extremity transplantation and may have implications for patient selection and counseling. Prognostic Study, Level V.
Alternative splicing in multiple sclerosis and other autoimmune diseases
2010
RNA Biology
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Poly-G runs constitute splicing enhancer elements and are important regulators of splicing, which are known to serve as binding sites for hnRNP H; recent work has shown that the activity of the poly-G ...
Two of these G-rich elements, named G 1 and M2, bind hnRNPs H and F, which recruit U1 snRNP to the DM20 5' splice site and regulate the PLP/DM20 ratio. 89 These mechanistic data, although not yet connected ...
doi:10.4161/rna.7.4.12301
pmid:20639696
pmcid:PMC3070910
fatcat:z5h3womh6nhnxj2kizyu7q7poi
Formate: an essential metabolite, a biomarker, or more?
2013
Clinical Chemistry and Laboratory Medicine
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Plasma and urinary formate concentrations were recently found to be elevated during vitamin B12 and folate deficiencies. It was proposed that formate may be a valuable biomarker of impaired one-carbon metabolism. Formate is an essential intermediary metabolite in folate-mediated one-carbon metabolism and, despite its importance, our knowledge of its metabolism is limited. Formate can be produced from several substrates (e.g., methanol, branched chain fatty acids, amino acids), some reactions
doi:10.1515/cclm-2012-0552
pmid:23241677
fatcat:bqabt4tuyjb4zol5khvo3skpxm
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... ng folate-dependent while others are not. Formate removal proceeds via two pathways; the major one being folate-dependent. Formate is a potentially toxic molecule and we suggest that formate may play a role in some of the pathologies associated with defective one-carbon metabolism.
Human centromere repositioning within euchromatin after partial chromosome deletion
2016
Chromosome Research
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One microgram of chromatin was incubated with 5μg of polyclonal mouse Cenp-A antibodies (AP601); ChIPs for each antibody were performed in duplicate per sample (WT or MUT). ...
doi:10.1007/s10577-016-9536-6
pmid:27581771
pmcid:PMC5366066
fatcat:bgaosdymsrafxb6kxcflylhuny
Military Applications of Augmented Reality
[chapter]
2011
Handbook of Augmented Reality
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Genetic predisposition of behavioral response
2014
Proceedings of the National Academy of Sciences of the United States of America
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Further, the level of facial recognition was dependent on the number of inherited alleles; for example, individuals who had the AA genotype from the A/G SNP had the worst facial recognition score. ...
doi:10.1073/pnas.1323421111
pmid:24449895
pmcid:PMC3918768
fatcat:gha6ynvlyna3vafpw5seynwiyq
A NEPTUNE-MASS PLANET ORBITING THE NEARBY G DWARF HD 16417
2009
Astrophysical Journal
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Interestingly, the distribution of detected sub-25 Mearth planets over the spectral types G, K and M is almost uniform. ...
Fiegert, and G. Schaffer. Facility: AAT, Keck:I ...
And second that current planet search target lists are dominated by G-dwarfs. ...
doi:10.1088/0004-637x/697/2/1263
fatcat:ne7sktf7cffjhozycgal2q3rk4
Kissing G Domains of MnmE Monitored by X-Ray Crystallography and Pulse Electron Paramagnetic Resonance Spectroscopy
2009
PLoS Biology
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Previous models suggested the protein to be a multidomain protein whose G domains contact each other in a nucleotide dependent manner. ...
MnmE, which is involved in the modification of the wobble position of certain tRNAs, belongs to the expanding class of G proteins activated by nucleotide-dependent dimerization (GADs). ...
This shows that the G domains adapt a closed conformation as observed in the GDP-AlF x -complexed G domain structure. ...
doi:10.1371/journal.pbio.1000212
pmid:19806182
pmcid:PMC2749940
fatcat:tkahuzpyofalpdkpnyrw2ikfpe
Magnetic control of a meta-molecule
2013
Optics Express
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Unlike spin-waves and MSSWs these are characterized by a negative group velocity ν g = ∂ω/∂k s < 0: hence the description 'backwards'. ...
Dimensions of, a = 3.93 mm, c = 0.375 mm, d = 0.45 mm, g = 0.69 mm. Field direction as shown by Ba.
Fig. 2 . 2 The (ν-Ba) map of the YIG-SRR anti-crossing modes. ...
doi:10.1364/oe.21.001456
pmid:23389127
fatcat:x2ggs2bc4vffpislsesmxurnmq
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