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The Role of the Microenvironment – Models for the Study of Melanoma [chapter]

Lucia Speroni, Victoria de los Angeles Bustuoabad, Julieta Gasparri, Nadia Silvia, Silvia del Valle Alonso
2011 Breakthroughs in Melanoma Research  
(mean) (Speroni et al, 2009a) . Importantly the hosts of tumors in the dorsal region of the foot had a poor survival compared to the hosts of flank tumors (Speroni et al, 2009a) .  ...  Thus, we chose the dorsal region of the foot and compared it to the flank in terms of cellular influx and reactivity (Speroni et al, 2009a) .  ...  How to reference In order to correctly reference this scholarly work, feel free to copy and paste the following: Lucia Speroni, Victoria de los Angeles Bustuoabad, Julieta Gasparri, Nadia Silvia Chiaramoni  ... 
doi:10.5772/20849 fatcat:ayqvjfgewnh6bgascoelruf5ou

Purification and crystallization of Kokobera virus helicase

Luigi De Colibus, Silvia Speroni, Bruno Coutard, Naomi L. Forrester, Ernest Gould, Bruno Canard, Andrea Mattevi
2007 Acta Crystallographica. Section F : Structural Biology and Crystallization Communications  
Kokobera virus is a mosquito-borne flavivirus belonging, like West Nile virus, to the Japanese encephalitis virus serocomplex. The flavivirus genus is characterized by a positive-sense single-stranded RNA genome. The unique open reading frame of the viral RNA is transcribed and translated as a single polyprotein which is post-translationally cleaved to yield three structural and seven nonstructural proteins, one of which is the NS3 gene that encodes a C-terminal helicase domain consisting of
more » ... amino acids. Helicase inhibitors are potential antiviral drugs as the helicase is essential to viral replication. Crystals of the Kokobera virus helicase domain were obtained by the hanging-drop vapourdiffusion method. The crystals belong to space group P3 1 21 (or P3 2 21), with unit-cell parameters a = 88.6, c = 138.6 Å , and exhibit a diffraction limit of 2.3 Å .
doi:10.1107/s1744309107005283 pmid:17329812 pmcid:PMC2330173 fatcat:xd6fjqfuf5aorkx2sfnettokf4

rec-Y2H matrix screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins [article]

Benjamin Lang, Jae-Seong Yang, Mireia Garriga-Canut, Silvia Speroni, Maria Gili, Gian Gaetano Tartaglia, Sebastian P Maurer
2020 bioRxiv   pre-print
RNA-binding proteins (RBPs) are crucial factors of post-transcriptional gene regulation and their modes of action are intensely investigated. At the center of attention are RNA motifs that guide where RBPs bind. However, sequence motifs are often poor predictors of RBP-RNA interactions in vivo. It is hence believed that many RBPs recognize RNAs as complexes, to increase specificity and regulatory potential. To probe the potential for complex formation among RBPs, we assembled a library of 978
more » ... mmalian RBPs and used rec-Y2H screening to detect direct interactions between RBPs, sampling >1M possible interactions. We discovered 1994 new interactions and demonstrate that our screening detects interactions between RBPs that bind RNA in proximity. We further find that the mRNA binding region preferences of an RBP can deviate, depending on its adjacently binding interaction partner. Finally, we reveal novel RBP interaction networks among major RNA processing steps and show that RBP mutations observed in cancer rewire spliceosomal interaction networks.
doi:10.1101/2020.09.14.296160 fatcat:rltes4wigvgyjkci2uwe7cba6a

L, P-/Q- and T-type Ca2+ Channels in Smooth Muscle Cells from Human Umbilical Artery

Silvia Salemme, Alejandro Rebolledo, Francisco Speroni, Silvana Petruccelli, Verónica Milesi
2007 Cellular Physiology and Biochemistry  
Salemme/Rebolledo/Speroni/Petruccelli/Milesi Cell Physiol Biochem 2007;20:55-64 Ca 2+ Channels in Human Umbilical ArteryCell Physiol Biochem 2007;20:55-64  ... 
doi:10.1159/000104153 pmid:17595515 fatcat:kkbu4xhegvaaxcbdpjoad5tx2y

Matrix-screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins

Benjamin Lang, Jae-Seong Yang, Mireia Garriga-Canut, Silvia Speroni, Moritz Aschern, Maria Gili, Tobias Hoffmann, Gian Gaetano Tartaglia, Sebastian P Maurer
2021 Nucleic Acids Research  
RNA-binding proteins (RBPs) are crucial factors of post-transcriptional gene regulation and their modes of action are intensely investigated. At the center of attention are RNA motifs that guide where RBPs bind. However, sequence motifs are often poor predictors of RBP-RNA interactions in vivo. It is hence believed that many RBPs recognize RNAs as complexes, to increase specificity and regulatory possibilities. To probe the potential for complex formation among RBPs, we assembled a library of
more » ... 8 mammalian RBPs and used rec-Y2H matrix screening to detect direct interactions between RBPs, sampling > 600 K interactions. We discovered 1994 new interactions and demonstrate that interacting RBPs bind RNAs adjacently in vivo. We further find that the mRNA binding region and motif preferences of RBPs deviate, depending on their adjacently binding interaction partners. Finally, we reveal novel RBP interaction networks among major RNA processing steps and show that splicing impairing RBP mutations observed in cancer rewire spliceosomal interaction networks. The dataset we provide will be a valuable resource for understanding the combinatorial interactions of RBPs with RNAs and the resulting regulatory outcomes.
doi:10.1093/nar/gkab490 pmid:34133714 fatcat:6lpemmmyzvfqpgijn4bzquo26a

Structure and Non-Structure of Centrosomal Proteins

Helena G. Dos Santos, David Abia, Robert Janowski, Gulnahar Mortuza, Michela G. Bertero, Maïlys Boutin, Nayibe Guarín, Raúl Méndez-Giraldez, Alfonso Nuñez, Juan G. Pedrero, Pilar Redondo, María Sanz (+14 others)
2013 PLoS ONE  
Here we perform a large-scale study of the structural properties and the expression of proteins that constitute the human Centrosome. Centrosomal proteins tend to be larger than generic human proteins (control set), since their genes contain in average more exons (20.3 versus 14.6). They are rich in predicted disordered regions, which cover 57% of their length, compared to 39% in the general human proteome. They also contain several regions that are dually predicted to be disordered and
more » ... oil at the same time: 55 proteins (15%) contain disordered and coiled-coil fragments that cover more than 20% of their length. Helices prevail over strands in regions homologous to known structures (47% predicted helical residues against 17% predicted as strands), and even more in the whole centrosomal proteome (52% against 7%), while for control human proteins 34.5% of the residues are predicted as helical and 12.8% are predicted as strands. This difference is mainly due to residues predicted as disordered and helical (30% in centrosomal and 9.4% in control proteins), which may correspond to alpha-helix forming molecular recognition features (a-MoRFs). We performed expression assays for 120 fulllength centrosomal proteins and 72 domain constructs that we have predicted to be globular. These full-length proteins are often insoluble: Only 39 out of 120 expressed proteins (32%) and 19 out of 72 domains (26%) were soluble. We built or retrieved structural models for 277 out of 361 human proteins whose centrosomal localization has been experimentally verified. We could not find any suitable structural template with more than 20% sequence identity for 84 centrosomal proteins (23%), for which around 74% of the residues are predicted to be disordered or coiled-coils. The three-dimensional models that we built are available at
doi:10.1371/journal.pone.0062633 pmid:23671615 pmcid:PMC3650010 fatcat:j75hiynby5bltoklr2ddfllqoa

Environmental effectiveness of GAEC cross-compliance standard 1.1c 'Maintenance of farm channel networks and field convexity' and economic evaluation of the competitiveness gap for farmers

Paolo Bazzoffi, Sergio Pellegrini, Silvia Carnevale, Lamberto Borrelli, Marisanna Speroni, Lorenzo Furlan, Francesca Chiarini, Michele Colauzzi, Francesco Fracasso, Marco Fedrizzi, Mirko Guerrieri, Mauro Pagano (+2 others)
2016 Italian Journal of Agronomy  
CREA-ING: Impostazione metodologica del monitoraggio del differenziale di competitività, applicazione della metodologia di rilievo dei Silvia Andrea Marco Franco di coltivazione: Spessore minimo  ... 
doi:10.4081/ija.2015.707 fatcat:ddwu5tdykbelljic7egs5l5j7e

Structure and functionality in flavivirus NS-proteins: Perspectives for drug design

Michela Bollati, Karin Alvarez, René Assenberg, Cécile Baronti, Bruno Canard, Shelley Cook, Bruno Coutard, Etienne Decroly, Xavier de Lamballerie, Ernest A. Gould, Gilda Grard, Jonathan M. Grimes (+17 others)
2010 Antiviral Research  
., 2007b; 3.1 Å) and KOKV (Speroni et al., 2008; 2.1 Å) .  ...  A similar role in coupling the ATPase and RNA unwinding activities is played by the residues of the Ia motif, as gathered from mutagenesis studies on the KOKV NS3Hel (Speroni et al., 2008) .  ... 
doi:10.1016/j.antiviral.2009.11.009 pmid:19945487 pmcid:PMC3918146 fatcat:u6qy2vnlfjagbloy3ymwthmxze

Environmental effectiveness of GAEC cross-compliance Standard 3.1 'Ploughing in good soil moisture conditions' and economic evaluation of the competitiveness gap for farmers

Rosa Francaviglia, Antonio Melchiorre Carroni, Paola Ruda, Mauro Salis, Paolo Bazzoffi, Silvia Carnevale, Andrea Rocchini, Antonio Troccoli, Mario Russo, Marisanna Speroni, Lamberto Borrelli, Giovanni Cabassi (+27 others)
2015 Italian Journal of Agronomy  
<p>Within the MO.NA.CO. Project the environmental effectiveness of GAEC cross-compliance Standard 3.1 'Ploughing in good soil moisture conditions' was evaluated, as well as the economic evaluation of the competitiveness gap for farmers which conform or do not conform to cross-compliance. The monitoring has been carried out at nine experimental farms with different pedoclimatic characteristics, where some indicators of soil structure degradation have been evaluated, such as bulk density, packing
more » ... density and surface roughness of the seedbed, and the crop productive and qualitative parameters. In each monitoring farm two experimental plots have been set up: factual with soil tillage at proper water content (tilth), counterfactual with soil tillage at inadequate water content (no tilth). The monitoring did not exhibit univocal results for the different parameters, thus the effectiveness of the Standard 3.1 is 'contrasting' (class of merit B), and there was an evident practical problem to till the soil at optimum water content, even in controlled experimental condition. Bulk density was significantly lower in the factual treatment although in soils with very different textures (sandy-loam and clayey). Packing density (PD) showed a high susceptibility to compaction in soils with low PD and medium texture. The tortuosity index, indicating the roughness of the seedbed, was lower and generally significantly different in the factual treatment. Results showed that the ploughing done in excessive soil moisture conditions is more expensive due to the increased force of traction of the tractor, which causes an increase in slip of the tractor wheels, with a speed reduction and increase in the working times and fuel consumption. Moreover, the crop yield is also reduced considerably according to the cultivated species.</p>
doi:10.4081/ija.2015.695 fatcat:ii2gmopbvjdbxndrq3etxlggqu

Sommario

-- --
2019 Studi giraldiani  
GIOVAN BATTISTA GIRALDI CINTHIO VALENTINA GALLO, Sull'epistola latina a Sperone Speroni attribuita a Giovan Battista Giraldi Cinthio 233 CORINNE LUCAS FIORATO, Appunti sulle immagini negli scritti  ...  di Giraldi Cinthio 265 SUSANNA VILLARI, Il rovesciamento del comico in tragedia: Silvia e Silla («Ecatommiti», I 10) e altri spunti esemplari 295 CARLA MOLINARI, Giraldi annotatore dell'«Orlando  ... 
doi:10.6092/2421-4191/2019.5.1-4 doaj:1d69f5a792664bf483590fb71d546a7b fatcat:dn4lyyacsffszdfp6dou2bvsoa

Environmental effectiveness of GAEC cross-compliance standard 2.2 "Maintaining the level of soil organic matter through crop rotation" and economic evaluation of the competitiveness gap for farmers

Lamberto Borrelli, Roberta Farina, Paolo Bazzoffi, Antonio Melchiorre Carroni, Paola Ruda, Mauro Salis, Silvia Carnevale, Andrea Rocchini, Nino Virzì, Francesco Intrigliolo, Massimo Palumbo, Michele Cambrea (+39 others)
2015 Italian Journal of Agronomy  
Within the Project MO.NA.CO was evaluated the Environmental effectiveness of GAEC cross-compliance standard 2.2 "Maintaining the level of soil organic matter through crop rotation" and economic evaluation of the competitiveness gap for farmers who support or not the cross-compliance regime. The monitoring was performed in nine experimental farms of the Council for Agricultural Research and Economics (CREA) distributed throughout Italy and with different soil and climatic conditions. Were also
more » ... aluated the soil organic matter and some yield parameters, in a cereal monocropping (treatment counterfactual) and a two-year rotation cereal-legume or forage (treatment factual). The two-years application of the standard "crop rotations" has produced contrasting results with regards to the storage of soil organic matter through crop rotation and these were not sufficient to demonstrate a statistically significant effect of treatment in any of the farms considered in monitoring, only in those farms subjected to more years of monitoring was recorded only a slight effect of the standard as a trend. The variations of organic matter in soils in response to changes in the culture technique or in the management of the soil may have long lag times and two years of time are not sufficient to demonstrate the dynamics of SOM associated with the treatment, also in consideration of the large inter annual variability recorded in different monitored sites.
doi:10.4081/ija.2015.689 fatcat:f57cjdu33badnlmdkjnp46x6ey

Page 30 of Nuova Antologia, Revista Di Lettere, Scienze Ed Arti Vol. 141, Issue [page]

1895 Nuova Antologia, Revista Di Lettere, Scienze Ed Arti  
; e in Tirsi cantore di Corte, esso medesimo il poeta; rimanendo i due amanti, il timido e desideroso Aminta e Silvia ritrosa vergine, sfumati in una idealità vaga, che tut- tavia non vieta anche alle  ...  sperta e lusinghiera Bendidio, in Licori; il vecchio se- gretario Giambatista Pigna, suo corteggiatore, nel savio Elpino; Batista Guarini, in Batto; nel maligno Mopso, il dottissimo mes- sere Sperone Speroni  ... 

Coronavirus Disease 2019: COSeSco - A Risk Assessment Score to Predict the Risk of Pulmonary Sequelae in COVID-19 Patients

Marina Aiello, Laura Marchi, Luigino Calzetta, Silvia Speroni, Annalisa Frizzelli, Marianna Ghirardini, Valentina Celiberti, Nicola Sverzellati, Maria Majori, Pier Anselmo Mori, Silvia Ranzieri, Roberta Pisi (+3 others)
2021
The presence of interstitial pneumonia in coronavirus disease 2019 (COVID-19) patients, as diagnosed through laboratory, functional, and radiological data, provides potential predicting factors of pulmonary sequelae. The objectives were the creation of a risk assessment score for pulmonary sequelae at high-resolution computed tomography (HRCT) through the assessment of laboratory data, lung function, and radiological changes in patients after the onset of COVID-19 interstitial pneumonia and the
more » ... identification of predictive factors. We enrolled 121 subjects hospitalized due to COVID-19 pneumonia in our study. Clinical features, Charlson Comorbidity Index (CCI) score, HRCT score, and blood chemistry data at hospital admission, as well as HRCT score, pulmonary function testing values, exercise capacity by means of a 6-Minute Walk Test (6MWT), and dyspnea perception by the modified Medical Research Council (mMRC) at 4-month follow-up, were all recorded. The variables were elaborated in order to create a predictive model to identify patients at high risk of pulmonary sequelae at HRCT. At the time of follow-up visit, 63% of patients had functional abnormality (diffusion lung capacity and/or total lung capacity <80% of predicted). Age, BMI, CCI, D-dimer, 6MWT, and mMRC were included in the COVID-19 Sequelae Score (COSeSco, ranging 0-15), which was able to individuate COVID-19 patients with radiologic sequelae (HRCT score >10%) at follow-up. The most revelatory COSeSco value that was found to intercept the highest sensitivity (100%) and specificity (77%) was 2. The COSeSco - comprising age, BMI, comorbidities, D-dimer, walking distance, and dyspnea perception - makes it possible to identify particularly at-risk COVID-19 patients who are likely to develop pulmonary sequelae assessed by HRCT.
doi:10.1159/000519385 pmid:34673642 pmcid:PMC9059039 fatcat:wjjhxyhkmvhy7b6xaiayz4vor4

Page 25 of Nuova Antologia, Revista Di Lettere, Scienze Ed Arti Vol. 139, Issue [page]

1895 Nuova Antologia, Revista Di Lettere, Scienze Ed Arti  
I satiri nell’Eg/e sono tutto; ma il satiro dell’ Amz7n/a discende più veramente da quello del Sacrificio di Agostino Beccari; come l’ idea di Silvia legata all’ albero e liberata da Aminta appar suggerita  ...  Tale verseg- giatura il Tasso dedusse dalla Canace di Sperone Speroni, pubbli- cata la prima volta nel 1546, e ne dedusse anche un verso intero (Pianti, sospiri e dimandar mercede) (2). Non altro.  ... 

Quel homme de lettres fut Torquato Tasso à la cour de Ferrare ? Sur les enjeux des clés poétiques de l'Aminta (1573)

Laurence Giavarini
2004 Littératures classiques  
Aucune clé n'est donnée pour Aminta et Silvia, les héros de la « favola boscarreccia ».  ...  D'autre part, elle propose des clés nouvelles pour le couple de bergers amoureux, Silvia et Aminta : Silvia-Marfisa, fille aînée de Francesco d'Este, l'oncle du duc, et Aminta-Alfonsino, premier né de  ... 
doi:10.3917/licla.054.0205 fatcat:fi4m5duw7rethfplsrftaj6baa
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