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BACKGROUND AND OBJECTIVE The novel coronavirus (SARS-Cov-2) infected coronavirus disease 2019 (COVID-19) was broken out in Wuhan and Hubei province for more than a month. It severely threats people's health of thousands in Chin and even other countries. In order to prevent its wide spread, it is necessary to understand the development of the epidemic with precise mathematical language. METHODS The various data of novel coronavirus pneumonia were collected from the official websites of thedoi:10.1101/2020.02.21.20026229 fatcat:ne3t5v7agfapngqntgpzpsuyi4
more »... al Health Committee of the People's Republic of China. According to epidemic and administrative division, three groups were divided to analyze the data, Hubei Province (including Wuhan), nationwide without Hubei and Henan Province. With classic SIR models, the fitting epidemiological curves of incidence have made, and basic reproduction number (R0) was also calculated as well. Therefore the disease's infection intensity, peak time and the epidemiological end time can be deduced.
With variable segment roles, three isomers exhibited different packing modes, nanocrystal morphologies and photoluminescence properties.doi:10.1039/c8nr02956c pmid:29987277 fatcat:xotffkew35dabelakfcoz2yvny
Selenoprotein S (SelS) is a transmembrane protein that is expressed in the liver, skeletal muscle, adipose tissue, pancreatic islets, kidney, and blood vessels. In addition to its transmembrane localization, SelS is also secreted from hepatoma HepG2 cells (but not L6 skeletal muscle cells, 3T3-L1 adipocytes, Min6 pancreatic β cells and human embryonic kidney 293 cells) and has been detected in the serum of some human subjects, with a detection rate of 31.1 %. These findings prove that serumdoi:10.1186/s12933-016-0388-3 pmid:27121097 pmcid:PMC4849094 fatcat:uxxarknndfbkvlcdupsz3yt3li
more »... is secreted by hepatocytes. However, whether vascularly expressed SelS can be secreted has not been reported. Transmembrane SelS has been suggested to play different roles in the pathogenesis and progression of diabetes mellitus (DM) and atherosclerosis (AS), but the association of secreted SelS with DM and macroangiopathy remains unclear. Research design and methods: Supernatants were collected from human umbilical vein endothelial cells (HUVECs), human aortic vascular smooth muscle cells (HA/VSMCs) and human hepatoma HepG2 cells that were untransfected or transfected with the indicated plasmid and concentrated for western blotting. Serum samples were collected from 158 human subjects with or without type 2 DM (T2DM) and/or AS. Serum SelS levels were measured using an enzyme-linked immunosorbent assay. Results: Secreted SelS was only detected in the supernatants of hepatoma HepG2 cells. The SelS detection rate among the 158 human serum samples was 100 %, and the average SelS level was 64.81 ng/dl. The serum SelS level in the isolated DM subjects was lower than the level in the healthy control subjects (52.66 ± 20.53 vs 70.40 ± 21.38 ng/ dl). The serum SelS levels in the DM complicated with SAS subjects (67.73 ± 21.41 ng/dl) and AS subjects (71.69 ± 27.00 ng/dl) were significantly increased compared with the serum SelS level in the isolated DM subjects. There was a positive interaction effect between T2DM and AS on the serum SelS level (P = 0.002). Spearman correlation analysis showed that the serum SelS level was negatively correlated with fasting plasma glucose. Conclusions: Vascular endothelial and vascular smooth muscle cells could not secrete SelS. Serum SelS was primarily secreted by hepatocytes. SelS was universally detected in human serum samples, and the serum SelS level was associated with T2DM and its macrovascular complications. Thus, regulating liver and serum SelS levels might become a new strategy for the prevention and treatment of DM and its macrovascular complications.
AbstractGenome-wide accurate identification and quantification of full-length mRNA isoforms is crucial for investigating transcriptional and post-transcriptional regulatory mechanisms of biological phenomena. Despite continuing efforts in developing effective computational tools to identify or assemble full-length mRNA isoforms from next-generation RNA-seq data, it remains a challenge to accurately identify mRNA isoforms from short sequence reads due to the substantial information loss indoi:10.1101/437350 fatcat:xw42xdujdzcwdc4on2bloyq6p4
more »... q experiments. Here we introduce a novel statistical method, AIDE (Annotation-assisted Isoform Discovery and abundance Estimation), the first approach that directly controls false isoform discoveries by implementing the statistical model selection principle. Solving the isoform discovery problem in a stepwise and conservative manner, AIDE prioritizes the annotated isoforms and precisely identifies novel isoforms whose addition significantly improves the explanation of observed RNA-seq reads. We evaluate the performance of AIDE based on multiple simulated and real RNA-seq datasets followed by a PCR-Sanger sequencing validation. Our results show that AIDE effectively leverages the annotation information to compensate the information loss due to short read lengths. AIDE achieves the highest precision in isoform discovery and the lowest error rates in isoform abundance estimation, compared with three state-of-the-art methods Cufflinks, SLIDE, and StringTie. As a robust bioinformatics tool for transcriptome analysis, AIDE will enable researchers to discover novel transcripts with high confidence.
Quetiapine is an atypical antipsychotic drug that can be used to treat mental disorders, including schizophrenia, bipolar disorder and Alzheimer's disease. Quetiapine is mainly converted into the active metabolites of norquetiapine and 7-hydroxyquetiapine by the liver enzymes CYP3A4 and CYP2D6. In this study, liquid-liquid extraction (LLE) was used as a sample pretreatment method to eliminate interferences in plasma. tert-Butyl methyl ether was chosen as the extraction solvent. Field-enhanceddoi:10.38212/2224-6614.3378 pmid:35649137 fatcat:curroesjjfegliffcckujl37fi
more »... mple injection (FESS), an online preconcentration technique, was used to analyze quetiapine and its metabolites norquetiapine and 7-hydroxyquetiapine in plasma. The optimal separation condition was 120 mM phosphate (pH 4.0) containing 0.005% (w/v) polyvinyl pyrrolidone and 40% (v/v) methanol. The methanol plug was 0.3 psi for 6 s, the sample was electrokinetic injection by 10 kV for 60 s at positive polarity, and the separation voltage was set at 26 kV. In this experiment, quetiapine, norquetiapine and 7-hydroxyquetiapine were successfully extracted from plasma by the LLE method and stacking and separated by FESS within 15 min. The limits of detection (S/N = 3) of quetiapine, norquetiapine and 7-hydroxyquetiapine were 0.25 ng/mL, 0.50 ng/mL and 1.00 ng/mL, respectively. The linear ranges of quetiapine, norquetiapine and 7-hydroxyquetiapine were 3-120 ng/mL and the correlation coefficients were 0.999. Compared with that of the traditional capillary zone electrophoresis method, the sensitivity enrichment of analytes was 463-835-fold. The optimal experimental conditions were successfully applied to the analysis of plasma samples from patients taking quetiapine for the treatment of schizophrenia.
To evaluate the role of serum cytokines in the pathogenesis of respiratory syncytial virus (RSV) infection in infants with low birth weight (LBW). Methods: A prospective observational study was performed, and hospitalized children with lower respiratory tract infection (LRTI) were recruited. Three hundred fifty-eight patients < 1 year met the inclusion criteria: 116 patients had only RSV infection (RSV group); 242 patients had no RSV or other specific pathogen (non-RSV group). Serumdoi:10.1186/s12887-017-0961-2 pmid:29246125 pmcid:PMC5732452 fatcat:xojzyioaazg6lkv2irqyvzavbq
more »... 2 (IL-2), IL-4, IL-6, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were detected through flow cytometry. Results: No significant differences in serum IL-2, 4, 6, 10, and IFN-γ levels were observed between the RSV and non-RSV groups. For RSV infected infants with or without wheezing, delivery mode had no obvious effect on the changes of serum cytokine levels. However, the level of IL-6 in the RSV-infected infants with LBW was significantly higher than that in infants with normal birth weight. Conclusions: Serum IL-6 level was significantly increased in RSV infected infants with LBW. It is likely that the specific serum cytokine pattern will contribute to our understanding of the pathogenesis of RSV infections, especially in RSV-infected infants with LBW.
Telocytes (TCs) are a novel type of interstitial cells, which have been recently described in a large variety of cavitary and noncavitary organs. TCs have small cell bodies, and remarkably thin, long, and moniliform prolongations called telopodes (Tps). Until now, TCs have been found in various loose connective tissues surrounding the arterioles, venules, and capillaries, but as a histological cellular component, whether TCs exist in large arteries remains unexplored. Methods: TCs weredoi:10.4103/0366-6999.150102 pmid:25635430 pmcid:PMC4837865 fatcat:km6zy3aj4jat3or2myw5filmlu
more »... d by transmission electron microscope in the aortic arch of male C57BL/6 mice. Results: TCs in aortic arch had small cell bodies (length: 6.06-13.02 μm; width: 1.05-4.25 μm) with characteristics of specific long (7.74-39.05 μm), thin, and moniliform Tps; TCs distributed in the whole connective tissue layer of tunica adventitia: TCs in the innermost layer of tunica adventitia, located at the juncture between media and adventitia, with their long axes oriented parallel to the outer elastic membrane; and TCs in outer layers of tunica adventitia, were embedded among transverse and longitudinal oriented collagen fibers, forming a highly complex three-dimensional meshwork. Moreover, desmosomes were observed, serving as pathways connecting neighboring Tps. In addition, vesicles shed from the surface of TCs into the extracellular matrix, participating in some biological processes. Conclusions: TCs in aorta arch are a newly recognized complement distinct from other interstitial cells in large arteries, such as fibroblasts. And further biologically functional correlations need to be elucidated. Abstract
To work ef®ciently with decision support systems (DSS), most users bene®t from representation conversion, i.e. translating the speci®c outcome from the DSS, normally portrayed in a numerical format, into the universal language of the visual. In general, interpretation of data is much more intuitive if the results from the DSS are translated into charts, maps, and other graphical displays because visualization exploits our natural ability to recognize and understand visual patterns. In thisdoi:10.1016/s0950-7051(01)00104-6 fatcat:uu4iccbszbd6hmx65qolvtnofm
more »... we discuss the concept of visualization user interface (VUI) for DSS. A proprietary software system known as AniGraftool is introduced as an example of an information visualization application for DSS. In addition, a visualized information retrieval engine based on fuzzy control is proposed. q
The reason for this difference lies mainly in that only one interpolation is performed under the condition of openloop while two interpolations are performed under the condition of closed-loop. ... The maximum error brought by the statistical interpolator is about 7% while the maximum error brought by the linear interpolator is about 25% when the interpolated point lies in the center of the square ...doi:10.1364/oe.17.014649 pmid:19687943 fatcat:6ojwzptafrbethhmfobsaifdbm
Three new acetophenones, named cynwilforones A–C (1–3), together with cynandione A (4) were isolated from the root bark of Cynanchum wilfordii (Maxim.) Hemsl. Their structures were deduced based on spectroscopic analysis and chemical methods. Compounds 1 and 4 exhibited potential hypoglycemic effects through inhibition of hepatic gluconeogenesis by down-regulating the expression of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase. This is the first report that acetophenones from thedoi:10.6084/m9.figshare.5924524 fatcat:gsuhlni44va3pcqsriatcgwiga
more »... oot bark of C. wilfordii possesses potential hypoglycemic activity in vitro.
Melatonin is a hormone produced by the pineal gland. Given its capabilities of neuroprotection and low neurotoxicity, melatonin could be a therapeutic strategy for traumatic brain injury (TBI). The present study was conducted to determine the neuroprotective effects of melatonin on TBI-induced anxiety and the possible molecular mechanism. Rats were randomly divided into seven groups. The rodent model of TBI was established using the weight-drop method. Melatonin was administered bydoi:10.3892/etm.2022.11173 pmid:35261620 pmcid:PMC8855513 fatcat:2qksofitn5afjerzc4il4lt72i
more »... al injection at a dose of 10 mg/kg after TBI. H89 (0.02 mg/kg), a special protein kinase A (PKA) inhibitor, or dibutyryl-cyclic adenosine monophosphate (cAMP; 0.1 mg/kg), an activator of PKA, were administered by stereotactic injection of the brain to evaluate the roles of PKA and cAMP-response element-binding protein (CREB) in melatonin-related mood regulation, respectively. At 30 days post-TBI, the changes in anxiety-like behaviors in rats were measured using the open field and elevated plus maze tests. At 24 h post-TBI, the number of activated astrocytes and neuronal apoptosis were evaluated using immunofluorescence assay. The expression levels of inflammatory cytokines (TNF-α and IL-6) in the amygdala were measured using an enzyme-linked immunosorbent assay. The expression levels of PKA, phosphorylated (p)-PKA, CREB, p-CREB, NF-κB and p-NF-κB in the amygdala were detected using western blotting. It was revealed that melatonin partially reversed TBI-induced anxiety-like behavior in rats, and decreased the number of activated astrocytes and neuronal apoptosis in the amygdala induced by TBI. H89 partially blocked the neuroprotective effects of melatonin; while dibutyryl-cAMP not only reduced the H89-induced emotional disturbance but also enhanced the protective effects of melatonin against TBI. Overall, melatonin can alleviate TBI-induced anxiety-like behaviors in rats. Moreover, the underlying mechanism may be associated with the activation of the PKA/CREB signaling pathway.
The aim of this study was to explore the factors influencing the success rate of distal transradial access (dTRA) for coronary intervention. A total of 246 patients who underwent either coronary angiography or percutaneous coronary intervention in the Xuzhou Central Hospital were enrolled in this study. Access via the distal radial artery was the first choice for the procedure, and the success rate of this approach was recorded. All patients underwent color Doppler ultrasonography of the distaldoi:10.2147/rmhp.s357780 pmid:35444479 pmcid:PMC9013917 fatcat:dbarhtnblbcq3my7uj7kxf656a
more »... radial artery at the right anatomical snuff box in order to measure the artery diameter. The patients were divided into two groups depending on the success of the dTRA; 44 patients with unsuccessful punctures were assigned to the observation group and 202 patients with successful punctures acted as the control group. The basic clinical data of the two groups were recorded, and the differences between the two groups with respect to various indices were evaluated. Logistic regression analysis was carried out to explore the factors influencing the success rate of dTRA. Of the 246 patients, dTRA was achieved in 202, giving a puncture success rate of 82.11%. Logistic regression analysis showed that the success rate of dTRA was positively correlated with the diameter of the distal radial artery (odds ratio [OR] = 3.381, P = 0.005) and hypertension (OR = 2.427, P = 0.016), and negatively correlated with female gender (OR = 0.429, P = 0.036) and diabetes mellitus (OR = 0.325, P = 0.002). The results of this study suggest that hypertension and distal radial artery diameter have a positive effect on the success rate of dTRA, while diabetes and female gender have a negative effect.
IsoLasso (Li et al. 2011b) , SLIDE (Li et al. 2011a) , and CIDANE (Canzar et al. 2016) all build linear models, in which read counts are summarized as the response variable, and isoform abundances ... Hence, computational efficiency becomes an inevitable factor that every method must account for, and an effective isoform screening step is often needed to achieve accurate isoform discovery (Ye and Li ...doi:10.1101/gr.251108.119 pmid:31694868 pmcid:PMC6886511 fatcat:7thf45e5pjdsjeu5xra6d4bok4
Acknowledgements Author contribution Lian LIU, Chun-hong MA and Li-hui HAN designed the research; Ling-ling DONG, Ji-sheng LI, Chao DU and Shan XU performed the research; Li LI and Xiu-wen WANG contributed ... analytic tools; Lian LIU and Ji-sheng LI analyzed data and wrote the paper. ...doi:10.1038/aps.2012.30 pmid:22543706 pmcid:PMC4010376 fatcat:n2togt5shrgdxbcnak474zsipi
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