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Discovery of plasma protein biomarkers related to Alzheimers disease, sex and APOE genotype [article]

Scott B. Laffoon, James D Doecke, Anne M Roberts, Jennifer A Vance, Benjamin D Reeves, Kelly K Pertile, Rebecca L Rumble, Chris J Fowler, Brett Trounson, David Ames, Ralph Martins, Ashley I Bush (+4 others)
2020 medRxiv   pre-print
The Australian Imaging and Biomarker Lifestyle (AIBL) study of aging is designed to aid the discovery of biomarkers. The current study aimed to discover differentially expressed plasma proteins that could yield a blood-based screening tool for Alzheimers disease. To search for biomarkers and elucidate mechanisms of AD, we immuno-depleted the most abundant plasma proteins and pre-fractionated the remaining proteins by HPLC, prior to two-dimensional gel electrophoresis. The relative levels of
more » ... oximately 3,400 protein species resolved on the 2D gels were compared using in-gel differential analysis with spectrally resolved fluorescent protein detection dyes (ZdyesTM). Here we report on analysis of pooled plasma samples from an initial screen of a sex-matched cohort of 72 probable AD patients and 72 healthy controls from the baseline time point of AIBL. We report significant changes in variants of apolipoprotein E, haptoglobin, alpha-anti-trypsin, inter-alpha trypsin inhibitor, histidine-rich glycoprotein, and a protein of unknown identity. Alpha-1-anti-trypsin and alpha-1 anti-chymotrypsin demonstrated plasma concentrations that were dependent on APOE e4 allele dose. Our analysis also identified an association with the level of Vitamin D binding protein fragments and complement factor I with sex. We then conducted a validation study on individual samples using a targeted LC-MS/MS assay. This study indicates that a peripheral protein signature has potential to aid in the characterization of AD. We also found significant associations of protein levels with APOE genotype, indicating that differences in genotype influence the circulating abundances of proteins other than ApoE.
doi:10.1101/2020.04.15.20066647 fatcat:o2h7xz4obnab3fhetb4ldjpepm

Island networks: Transformations of inter-community social relationships in the Lesser Antilles at the advent of European colonialism

Corinne L. Hofman, Lewis Borck, Jason E. Laffoon, Emma R. Slayton, Rebecca B. Scott, Thomas W. Breukel, Catarina Guzzo Falci, Maroussia Favre, Menno L. P. Hoogland
2020 Journal of Island & Coastal Archaeology  
We thank Emma de Mooij for her editorial help and the reviewers and Scott Fitzpatrick for their useful comments on this paper. We specifically want to acknowledge the people of Grenada and St.  ...  Scott 1985) .  ...  Scott et al. 2018 ).  ... 
doi:10.1080/15564894.2020.1748770 fatcat:w5jmbcqivbbpjc7gjdo4igmati

Page 826 of Mechanical Engineering Vol. 79, Issue 8 [page]

1957 Mechanical Engineering  
1888 Parents, Mark and Sallie (Brown) Laffoon.  ...  Laffoon had been the author of more than 20 papers which appeared in publications of ASME.  ... 

Page 14 of Spectrum Vol. 7, Issue 1 [page]

1934 Spectrum  
Ruby Laffoon, Governor of S. H. Brown.  ...  Scott Harry Sparks L. J. Spencer H. B. Thornton S. B. Tracy Elmore Jack Thomas |. Sterling Towles J. B. Wicker Elmer G.  ... 

Page 35 of Bench & Bar Vol. 3, Issue 3 [page]

1939 Bench & Bar  
Scott Mays. Hon. Oliver Popplewell of Casey County is a candidate for Common- wealth’s Attorney for the 29th Dis- trict.  ...  Stanley is in Washington, and Ruby Laffoon is practicing law in Madi- sonville. Attorney Strother Haynes of Ash- land is the new president of the Lions Club at Ashland. Mr. G.  ... 

Page 35 of Bench & Bar Vol. 4, Issue 4 [page]

1940 Bench & Bar  
Former Governor Ruby Laffoon was special judge of the Marshall Cir- cuit Court in June. Mr. John Berry of the New Castle Bar was special judge of the Gallatin Circuit Court in June. Hon. B. N.  ...  Scott of Cave City has been named as City Judge pro tem. He resigned the post of City Attorney to accept the appointment. Attorney J. R. Whitlow has chosen Hodgenville as the place of his prac- tice.  ... 

Page 2156 of Public Health Reports Vol. 39, Issue 34 [page]

1924 Public Health Reports  
., preident, Fort Scott.” C. A. Laffoon, M. D., vice president, Easton, Henry D. Smith, M. D., Washington. C. A. Fisher, M. D., Paola. D. E. Smith, M. D., Kansas City. R. G. Klein, M. D., Dodge City.  ... 

Page 30 of Bench & Bar Vol. 1, Issue 3 [page]

1937 Bench & Bar  
Ex-Governor and Ex-Circuit Judge Ruby Laffoon served for a week as spe- cial judge at Madisonville, while the reg- ular Judge Charles H. Wilson was water- bound. JUDGE H. H.  ...  SCOTT has been elected President of the Campbell County Bar Association. Mr. Harry J. Luedeke has been elected Vice-President, Mr. Oscar H. Forster has been elected Treasurer, and Mr. Thomas F.  ... 

Page 757 of American Society of Civil Engineers. Proceedings of the American Society of Civil Engineers Vol. 45, Issue 6 [page]

1919 American Society of Civil Engineers. Proceedings of the American Society of Civil Engineers  
Laffoon. (27) June 14. Rehabilitation of New York Cotton Mill.* (27) June 14. Lighting and Control for Bridge Over the Columbia.* F.H. Murphy. (27) June14.  ...  Scott. (27) July 12. Standardized Industrial Lighting Systems.* W. L. Cornelison. (27) July 12. os in Obtaining Maximum Output from Hydro-Electric Plants. Fred A mer.  ... 

Analysis of plasma proteins using 2D gels and novel fluorescent probes: in search of blood based biomarkers for Alzheimer's disease

Scott B Laffoon, James D Doecke, Anne M Roberts, Jennifer A Vance, Benjamin D Reeves, Kelly K Pertile, Rebecca L Rumble, Chris J Fowler, Brett Trounson, David Ames, Ralph Martins, Ashley I Bush (+4 others)
2022
The Australian Imaging and Biomarker Lifestyle (AIBL) study of aging is designed to aid the discovery of biomarkers. The current study aimed to discover differentially expressed plasma proteins that could yield a blood-based screening tool for Alzheimer's disease. The concentration of proteins in plasma covers a vast range of 12 orders of magnitude. Therefore, to search for medium to low abundant biomarkers and elucidate mechanisms of AD, we immuno-depleted the most abundant plasma proteins and
more » ... pre-fractionated the remaining proteins by HPLC, prior to two-dimensional gel electrophoresis. The relative levels of approximately 3400 protein species resolved on the 2D gels were compared using in-gel differential analysis with spectrally resolved fluorescent protein detection dyes (Zdyes™). Here we report on analysis of pooled plasma samples from an initial screen of a sex-matched cohort of 72 probable AD patients and 72 healthy controls from the baseline time point of AIBL. We report significant changes in variants of apolipoprotein E, haptoglobin, α1 anti-trypsin, inter-α trypsin inhibitor, histidine-rich glycoprotein, and a protein of unknown identity. α1 anti-trypsin and α1 anti-chymotrypsin demonstrated plasma concentrations that were dependent on APOE ε4 allele dose. Our analysis also identified an association with the level of Vitamin D binding protein fragments and complement factor I with sex. We then conducted a preliminary validation study, on unique individual samples compared to the discovery cohort, using a targeted LC-MS/MS assay on a subset of discovered biomarkers. We found that targets that displayed a high degree of isoform specific changes in the 2D gels were not changed in the targeted MS assay which reports on the total level of the biomarker. This demonstrates that further development of mass spectrometry assays is needed to capture the isoform complexity that exists in theses biological samples. However, this study indicates that a peripheral protein signature has potential to aid in the characterization of AD.
doi:10.1186/s12953-021-00185-9 pmid:35081972 pmcid:PMC8790928 fatcat:2drk2sinbfgfnn3oiqglf5fco4

Page 277 of Entomological Society of America. Annals of the Entomological Society of America Vol. 55, Issue 3 [page]

1962 Entomological Society of America. Annals of the Entomological Society of America  
Laffoon, 1 female. Lourtstana: Baton Rouge, Amite River, 16 May 1947, W. W. Wirth, 8 males, 1 female. Kilbourne, 10 May 1947, W. W. Wirth, 1 male. MaAryLtanp: Cabin John, 10 Aug. 1916, R. M.  ...  Potomac River at Scott Run, Fairfax Co., 7 June 1955, W. W. Wirth and R. H. Jones, reared from sandy river margin, 11 males, 10 females. St. Elmo, June, F. C. Pratt, 4 females. male, 2 females.  ... 

DESCRIPTION OF A NEW SPECIES OF HESPERIAN FROM TEXAS

W. H. Edwards
1877 Canadian Entomologist  
Scott (1952) found that a single pair of flies would not mate or oviposit, but some fertile eggs were obtained from two pairs.  ...  Laffoon on Guadalcanal in 1944 represents another, apparently undescribed, species. Acropsilus protractus, new species MALE.-1.0 mm; wing I. 1 mm by 0.4 mm.  ... 
doi:10.4039/ent958-3 fatcat:rgcaqhoon5fzhhnaf7dzwqag7u

Page 1639 of JAMA: The Journal of the American Medical Association Vol. 146, Issue 17 [page]

1951 JAMA: The Journal of the American Medical Association  
, Ralph August, 859 Krahn, George Washington, 50 Krause, Charles Schurz, 50 Kremers, Alexander, 860 Kreuder, Henry, 861 Kriebs, Frank Joseph, 861 Krohn, Irwin Kjelland, 1336 Kyser, James Allen, 665 L Laffoon  ...  ., 860 Kaufmann, Eugene, 860 Kaveney, Irving Edward, 665 Keith, Frederick Scott, 744 Kelley, Erwin E 133¢ Kenworthey, William Bartle, 860 Kerns, Francis John, 490 Kerr, Alvin Robert, 389 Kerr, Frederick  ... 

Hard Times and New Deal in Kentucky, 1929-1939

David E. Conrad, George T. Blakey
1989 American Historical Review  
Scott Fitzgerald' s The Great Gats by, another popular novel of the day. Inside the Seelbach could be found many of the stereotypes of this boisterous age: flappers, jazz bands, and illicit liquor.  ...  Laffoon was well aware that federal politicians held him in contempt.  ...  Scott, 6 floods, (1927), 14; (1937), 59, 75, 130, 135, 189, 191-94 Florida, 192 Fort Knox, Kentucky, 74, 81-82, 193 Fort Thomas, Kentucky, 83, 87, 171 Frankfort, Kentucky, 20, 25-26, 35, 42, 44, 49-50,  ... 
doi:10.2307/1867001 fatcat:s54cwwhf4jfpplpvtlbsx5iete

Page 274 of POWER Vol. 76, Issue 5 [page]

1932 POWER  
Trnius OLseEn, 86, pioneer in the devel- opment of testing machinery in the United States, oldest living member of the Frank- lin Institute and holder of its John Scott and Eliot Cresson medals, founder  ...  Garrison, who has been chief en- gineer of the Central Life Insurance Co.’s building in Cincinnati, Ohio, for twenty years, has been appointed an aid-de-camp on the’ staff of Governor Ruby Laffoon of Kentucky  ... 
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