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Evaluation of Approaches to Identify the Targets of Cellular Immunity on a Proteome-Wide Scale
2011
PLoS ONE
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Herein, we report a novel approach for proteome-wide scale identification of the antigenic targets of T cell responses using IVTT products. ...
The recent elucidation of the genome, proteome and transcriptome of many of these complex pathogens provides the basis for rational vaccine design by identifying, on a proteome-wide scale, novel target ...
This is a favorable scenario for proteomic-wide scale cellular screening, as the use of highly diluted IVTT products is more cost-effective. ...
doi:10.1371/journal.pone.0027666
pmid:22096610
pmcid:PMC3214079
fatcat:m5pe57hk3vhb3or5rddswbbq2i
Mutating the anchor residues associated with MHC binding inhibits and deviates CD8+ T cell mediated protective immunity against malaria
2007
Molecular Immunology
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change one or more primary anchor residues of the immunodominant CD8+ T cell epitope on the Plasmodium yoelii circumsporozoite protein, and in vivo protective efficacy and immune responses against defined PyCSP ...
Our data demonstrate that protective immunity induced by PyCSP DNA vaccination is directed predominantly against the single immunodominant CD8+ epitope, and that although responses can be induced against ...
Large-scale preparations of the DNA constructs were purified by standard alkaline lysis followed by cesium chloride gradient centrifugation (Hedstrom and Doolan, 2002) . ...
doi:10.1016/j.molimm.2006.11.003
pmid:17169429
fatcat:cydd2cbnabartfza6pmi2nbsf4
Towards a Software Engineering Approach to Multi-Scale Modeling and Simulation
2016
International Journal of Software Engineering and Its Applications
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The proposed methodology relies on a sound integration of UML-MARTE, CSP-OZ, and PyCSP. We show, in this paper, how to exploit this similarity to bridge the gap between both approaches. ...
MUSCLE [9], a recent distributed multi-scale simulation framework, serves as a case study in this work. ...
built at the design level are translated into PyCSP code. ...
doi:10.14257/ijseia.2016.10.11.18
fatcat:2swrw2otnrdyrikvt6j4adq3ze
Identification of Pre-Erythrocytic Malaria Antigens That Target Hepatocytes for Killing In Vivo and Contribute to Protection Elicited by Whole-Parasite Vaccination
2014
PLoS ONE
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The scale indicates radiance expressed as p/s/cm 2 /sr. (B) Quantitation of the data shown in part A.
Figure 3 . 3 PyCSP-specific CD8 + T cells produce high levels of IFN-c. ...
The scale indicates radiance expressed as p/s/cm 2 /sr. (B) Kinetics of luciferase signal in live naïve BALB/c mice (shown as total flux p per second) during the course of the experiment. ...
doi:10.1371/journal.pone.0102225
pmid:25025375
pmcid:PMC4099202
fatcat:lq4pvriznjhcdj52tavwqd423e
Plasmid Vaccine Expressing Granulocyte-Macrophage Colony-Stimulating Factor Attracts Infiltrates Including Immature Dendritic Cells into Injected Muscles
2000
Journal of Immunology
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Scale bar indicates 200 m in A, 40 m in B and C, and 20 m in D and E.
. ...
Table I shows that coinjection of pPyCSP and pGM-CSF gave a substantial boost to the Ab response to PyCSP, as well as to the IFN-␥ responses to the CD4 (PyCSP 57-70 aa ) and CD8 (PyCSP 280 -88 aa ) T ...
doi:10.4049/jimmunol.165.7.3772
pmid:11034382
fatcat:uxi2bqrrznfvxknnv4gfqyhas4
Alphavirus Replicon Particles Are Highly Immunogenic in the Murine Malaria Model by Homologous or Heterologous Immunization
2008
The Open Vaccine Journal
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SC DDD empty IM RRR PyCSP SC RRR PyCSP IM DDD PyCSP IM RRD PyCSP SC/IM RRV PyCSP SC/IM DDV PyCSP IM Fig. (4). ...
delivery systems facilitate high level of antigen expression while retaining some of the attractive features of DNA vaccination technology, particularly the simplicity of design, modification, large-scale ...
doi:10.2174/1875035400801010027
fatcat:y3owrdmoubblrpd23btinul2nm
Chimeric Murine Polyomavirus Virus-Like Particles Induce Plasmodium Antigen-Specific CD8+ T Cell and Antibody Responses
2019
Frontiers in Cellular and Infection Microbiology
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Scale bar is 100 nm. induced by the CD8 VLP were similar to that induced by peptide in adjuvant. ...
PyCSP plasmid DNA (100 µg) primes followed by a s.c. boost of either pooled PyCSP VLPs (10 µg each) or pooled PyCSP peptides (30 µg each); or three homologous s.c. immunizations with pooled PyCSP VLPs ...
doi:10.3389/fcimb.2019.00215
fatcat:33wr2spubjgh7jlbtktpdmd7my
PYCSP3: Modeling Combinatorial Constrained Problems in Python
[article]
2021
arXiv
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t = [3 , 2.11 , 0.0141] print ( " t scaled : " , i n te ge r _s ca l in g ( t ))we obtain: t scaled:[30000, 21100, 141] ...
The function integer_scaling returns a list with all specified values after possibly converting them (when decimal) into integers by means of automatic scaling. ...
based on parallelism) in a safer manner
Main Module Interface In this section, we briefly review all components (constants, variables, functions) that are available from the main module of the library PyCSP ...
arXiv:2009.00326v3
fatcat:3rwe6smirvgezhlpcyw6nxhic4
Chimeric Virus-Like Particles and Capsomeres Induce Similar CD8+ T Cell Responses but Differ in Capacity to Induce CD4+ T Cell Responses and Antibody Responses
2020
Frontiers in Immunology
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Scale bar represents 10 µm. antibodies and their protective capacity has yet to be established. ...
DNA transfected A20s or with pooled PyCSP peptides ( Figure 1A) . ...
doi:10.3389/fimmu.2020.564627
pmid:33133076
pmcid:PMC7550421
fatcat:d6tb3tftp5akpfbdj5qpgmelvi
Vaccination with Lipid Core Peptides Fails to Induce Epitope-Specific T Cell Responses but Confers Non-Specific Protective Immunity in a Malaria Model
2012
PLoS ONE
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Epitopes from P. yoelii circumsporozoite protein (PyCSP) as follows: P1 = PyCSP 280-288 , SYVPSAEQI; P2 = PyCSP 280-295 , SYVPSAEQILEFVKQI; P3 = PyCSP 58-67 , IYNRNIVNRL; P4 = PyCSP 57-70 , KIYNRNIVNRLLGD ...
Synthetic peptide-based vaccines offer many advantages over whole-organism vaccines due their amenability to large-scale production, their well-defined composition and purity, and their suitability for ...
doi:10.1371/journal.pone.0040928
pmid:22936972
pmcid:PMC3427299
fatcat:77binrainzf4lbubfmihutamca
Novel malaria antigen Plasmodium yoelii E140 induces antibody-mediated sterile protection in mice against malaria challenge
2020
PLoS ONE
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Scale bar indicates 10 μm. ...
Scale bar indicates 10 μm. https://doi.org/10.1371/journal.pone.0232234.g001 PyCSP and challenged with 300 P. yoelii 17XNL sporozoites. ...
doi:10.1371/journal.pone.0232234
pmid:32407410
fatcat:tyhta56c55aobnkjqzlfmdo4b4
Transcriptionally active PCR for antigen identification and vaccine development: In vitro genome-wide screening and in vivo immunogenicity
2008
Molecular and biochemical parasitology (Print)
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However, the TAP approach is designed for high throughput in vitro screening studies, and not small-scale in vivo immunization studies. ...
T cell responses against PyCSP BALB/c mice were immunized IM with 100 or 50 μg plasmid DNA or 50 μg of TAP fragments encoding PyCSP 3 times at 3-week intervals. ...
doi:10.1016/j.molbiopara.2007.11.009
pmid:18164079
pmcid:PMC2699467
fatcat:qwtxpwvkmzdfpeomgnmx4ltzqu
High-Throughput Generation of P. falciparum Functional Molecules by Recombinational Cloning
2004
Genome Research
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Large-scale functional genomics studies for malaria vaccine and drug development will depend on the generation of molecular tools to study protein expression. ...
COS-7 cells were transfected with PyCSP and PfCSP DNA constructs in both VR1020 and VR1020-DV plasmids as described (Hedstrom et al. 1998 ). ...
These results may reflect the fact that genes from Panel A were selected prior to publication of large-scale gene expression profiles. ...
doi:10.1101/gr.2416604
pmid:15489329
pmcid:PMC528923
fatcat:ek3aao73fnhptk4yflvwtz7kai
Anti-TRAP/SSP2 monoclonal antibodies can inhibit sporozoite infection and enhance protection of anti-CSP monoclonal antibodies
[article]
2021
bioRxiv
pre-print
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In our in vivo Py inhibition studies, the combination of mAbs brought the total antibody dose to four times that of the anti-PyCSP antibody alone (100 μg/mouse of anti-PyCSP alone to 400 μg/mouse total ...
The interventions that have provided much of the previous progress, such as insecticidetreated bednets and large scale treatment programs, are highly susceptible to interruptions due to political or economic ...
doi:10.1101/2021.10.15.464611
fatcat:vpjeivmgebewpkxqro57du7aw4
Plasmodium Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole
2011
PLoS Pathogens
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Scale bars are equal to 0.5 mm. ...
Scale bars are equal to 0.5 mm. Abbreviations: PVM-Parasitophorous vacuole membrane, PV-Parasitophorous vacuole. ...
doi:10.1371/journal.ppat.1002197
pmid:21909259
pmcid:PMC3164628
fatcat:jgily2x4v5f3va4kzbv3sxdyau
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