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Evaluation of Approaches to Identify the Targets of Cellular Immunity on a Proteome-Wide Scale

Fernanda C. Cardoso, Joanne S. Roddick, Penny Groves, Denise L. Doolan, Vasee Moorthy
2011 PLoS ONE  
Herein, we report a novel approach for proteome-wide scale identification of the antigenic targets of T cell responses using IVTT products.  ...  The recent elucidation of the genome, proteome and transcriptome of many of these complex pathogens provides the basis for rational vaccine design by identifying, on a proteome-wide scale, novel target  ...  This is a favorable scenario for proteomic-wide scale cellular screening, as the use of highly diluted IVTT products is more cost-effective.  ... 
doi:10.1371/journal.pone.0027666 pmid:22096610 pmcid:PMC3214079 fatcat:m5pe57hk3vhb3or5rddswbbq2i

Mutating the anchor residues associated with MHC binding inhibits and deviates CD8+ T cell mediated protective immunity against malaria

C. Dobaño, A. McTague, A. Sette, S.L. Hoffman, W.O. Rogers, D.L. Doolan
2007 Molecular Immunology  
change one or more primary anchor residues of the immunodominant CD8+ T cell epitope on the Plasmodium yoelii circumsporozoite protein, and in vivo protective efficacy and immune responses against defined PyCSP  ...  Our data demonstrate that protective immunity induced by PyCSP DNA vaccination is directed predominantly against the single immunodominant CD8+ epitope, and that although responses can be induced against  ...  Large-scale preparations of the DNA constructs were purified by standard alkaline lysis followed by cesium chloride gradient centrifugation (Hedstrom and Doolan, 2002) .  ... 
doi:10.1016/j.molimm.2006.11.003 pmid:17169429 fatcat:cydd2cbnabartfza6pmi2nbsf4

Towards a Software Engineering Approach to Multi-Scale Modeling and Simulation

M. Maouche, M. Bettaz
2016 International Journal of Software Engineering and Its Applications  
The proposed methodology relies on a sound integration of UML-MARTE, CSP-OZ, and PyCSP. We show, in this paper, how to exploit this similarity to bridge the gap between both approaches.  ...  MUSCLE [9], a recent distributed multi-scale simulation framework, serves as a case study in this work.  ...  built at the design level are translated into PyCSP code.  ... 
doi:10.14257/ijseia.2016.10.11.18 fatcat:2swrw2otnrdyrikvt6j4adq3ze

Identification of Pre-Erythrocytic Malaria Antigens That Target Hepatocytes for Killing In Vivo and Contribute to Protection Elicited by Whole-Parasite Vaccination

Lin Chen, Gladys J. Keitany, Xiaohong Peng, Claire Gibson, Isaac Mohar, Marissa Vignali, Ian N. Crispe, Fusheng Huang, Ruobing Wang, Anne Charlotte Gruner
2014 PLoS ONE  
The scale indicates radiance expressed as p/s/cm 2 /sr. (B) Quantitation of the data shown in part A. Figure 3 . 3 PyCSP-specific CD8 + T cells produce high levels of IFN-c.  ...  The scale indicates radiance expressed as p/s/cm 2 /sr. (B) Kinetics of luciferase signal in live naïve BALB/c mice (shown as total flux p per second) during the course of the experiment.  ... 
doi:10.1371/journal.pone.0102225 pmid:25025375 pmcid:PMC4099202 fatcat:lq4pvriznjhcdj52tavwqd423e

Plasmid Vaccine Expressing Granulocyte-Macrophage Colony-Stimulating Factor Attracts Infiltrates Including Immature Dendritic Cells into Injected Muscles

D. Haddad, J. Ramprakash, M. Sedegah, Y. Charoenvit, R. Baumgartner, S. Kumar, S. L. Hoffman, W. R. Weiss
2000 Journal of Immunology  
Scale bar indicates 200 m in A, 40 m in B and C, and 20 m in D and E. .  ...  Table I shows that coinjection of pPyCSP and pGM-CSF gave a substantial boost to the Ab response to PyCSP, as well as to the IFN-␥ responses to the CD4 (PyCSP 57-70 aa ) and CD8 (PyCSP 280 -88 aa ) T  ... 
doi:10.4049/jimmunol.165.7.3772 pmid:11034382 fatcat:uxi2bqrrznfvxknnv4gfqyhas4

Alphavirus Replicon Particles Are Highly Immunogenic in the Murine Malaria Model by Homologous or Heterologous Immunization

C. Dobano, W. R. Weiss, K. I. Kamrud, J. D. Chulay, J. Smith, D. J. Carucci, D. L. Doolan
2008 The Open Vaccine Journal  
SC DDD empty IM RRR PyCSP SC RRR PyCSP IM DDD PyCSP IM RRD PyCSP SC/IM RRV PyCSP SC/IM DDV PyCSP IM Fig. (4).  ...  delivery systems facilitate high level of antigen expression while retaining some of the attractive features of DNA vaccination technology, particularly the simplicity of design, modification, large-scale  ... 
doi:10.2174/1875035400801010027 fatcat:y3owrdmoubblrpd23btinul2nm

Chimeric Murine Polyomavirus Virus-Like Particles Induce Plasmodium Antigen-Specific CD8+ T Cell and Antibody Responses

David J. Pattinson, Simon H. Apte, Nani Wibowo, Yap P. Chuan, Tania Rivera-Hernandez, Penny L. Groves, Linda H. Lua, Anton P. J. Middelberg, Denise L. Doolan
2019 Frontiers in Cellular and Infection Microbiology  
Scale bar is 100 nm. induced by the CD8 VLP were similar to that induced by peptide in adjuvant.  ...  PyCSP plasmid DNA (100 µg) primes followed by a s.c. boost of either pooled PyCSP VLPs (10 µg each) or pooled PyCSP peptides (30 µg each); or three homologous s.c. immunizations with pooled PyCSP VLPs  ... 
doi:10.3389/fcimb.2019.00215 fatcat:33wr2spubjgh7jlbtktpdmd7my

PYCSP3: Modeling Combinatorial Constrained Problems in Python [article]

Christophe Lecoutre, Nicolas Szczepanski
2021 arXiv   pre-print
t = [3 , 2.11 , 0.0141] print ( " t scaled : " , i n te ge r _s ca l in g ( t ))we obtain: t scaled:[30000, 21100, 141]  ...  The function integer_scaling returns a list with all specified values after possibly converting them (when decimal) into integers by means of automatic scaling.  ...  based on parallelism) in a safer manner Main Module Interface In this section, we briefly review all components (constants, variables, functions) that are available from the main module of the library PyCSP  ... 
arXiv:2009.00326v3 fatcat:3rwe6smirvgezhlpcyw6nxhic4

Chimeric Virus-Like Particles and Capsomeres Induce Similar CD8+ T Cell Responses but Differ in Capacity to Induce CD4+ T Cell Responses and Antibody Responses

David J. Pattinson, Simon H. Apte, Nani Wibowo, Tania Rivera-Hernandez, Penny L. Groves, Anton P. J. Middelberg, Denise L. Doolan
2020 Frontiers in Immunology  
Scale bar represents 10 µm. antibodies and their protective capacity has yet to be established.  ...  DNA transfected A20s or with pooled PyCSP peptides ( Figure 1A) .  ... 
doi:10.3389/fimmu.2020.564627 pmid:33133076 pmcid:PMC7550421 fatcat:d6tb3tftp5akpfbdj5qpgmelvi

Vaccination with Lipid Core Peptides Fails to Induce Epitope-Specific T Cell Responses but Confers Non-Specific Protective Immunity in a Malaria Model

Simon H. Apte, Penny L. Groves, Mariusz Skwarczynski, Yoshio Fujita, Chenghung Chang, Istvan Toth, Denise L. Doolan, Mauricio Martins Rodrigues
2012 PLoS ONE  
Epitopes from P. yoelii circumsporozoite protein (PyCSP) as follows: P1 = PyCSP 280-288 , SYVPSAEQI; P2 = PyCSP 280-295 , SYVPSAEQILEFVKQI; P3 = PyCSP 58-67 , IYNRNIVNRL; P4 = PyCSP 57-70 , KIYNRNIVNRLLGD  ...  Synthetic peptide-based vaccines offer many advantages over whole-organism vaccines due their amenability to large-scale production, their well-defined composition and purity, and their suitability for  ... 
doi:10.1371/journal.pone.0040928 pmid:22936972 pmcid:PMC3427299 fatcat:77binrainzf4lbubfmihutamca

Novel malaria antigen Plasmodium yoelii E140 induces antibody-mediated sterile protection in mice against malaria challenge

Emily C. Smith, Keith J. Limbach, Nonenipha Rangel, Kyosuke Oda, Jessica S. Bolton, Mengyan Du, Kalpana Gowda, Jianyang Wang, J. Kathleen Moch, Sharvari Sonawane, Rachel Velasco, Arnel Belmonte (+10 others)
2020 PLoS ONE  
Scale bar indicates 10 μm.  ...  Scale bar indicates 10 μm. PyCSP and challenged with 300 P. yoelii 17XNL sporozoites.  ... 
doi:10.1371/journal.pone.0232234 pmid:32407410 fatcat:tyhta56c55aobnkjqzlfmdo4b4

Transcriptionally active PCR for antigen identification and vaccine development: In vitro genome-wide screening and in vivo immunogenicity

David P. Regis, Carlota Dobaño, Paola Quiñones-Olson, Xiaowu Liang, Norma L. Graber, Maureen E. Stefaniak, Joseph J. Campo, Daniel J. Carucci, David A. Roth, Huaping He
2008 Molecular and biochemical parasitology (Print)  
However, the TAP approach is designed for high throughput in vitro screening studies, and not small-scale in vivo immunization studies.  ...  T cell responses against PyCSP BALB/c mice were immunized IM with 100 or 50 μg plasmid DNA or 50 μg of TAP fragments encoding PyCSP 3 times at 3-week intervals.  ... 
doi:10.1016/j.molbiopara.2007.11.009 pmid:18164079 pmcid:PMC2699467 fatcat:qwtxpwvkmzdfpeomgnmx4ltzqu

High-Throughput Generation of P. falciparum Functional Molecules by Recombinational Cloning

J. C. Aguiar
2004 Genome Research  
Large-scale functional genomics studies for malaria vaccine and drug development will depend on the generation of molecular tools to study protein expression.  ...  COS-7 cells were transfected with PyCSP and PfCSP DNA constructs in both VR1020 and VR1020-DV plasmids as described (Hedstrom et al. 1998 ).  ...  These results may reflect the fact that genes from Panel A were selected prior to publication of large-scale gene expression profiles.  ... 
doi:10.1101/gr.2416604 pmid:15489329 pmcid:PMC528923 fatcat:ek3aao73fnhptk4yflvwtz7kai

Anti-TRAP/SSP2 monoclonal antibodies can inhibit sporozoite infection and enhance protection of anti-CSP monoclonal antibodies [article]

Brandon K. Wilder, Vladimir Vigdorovich, Sara Carbonetti, Nana Minkah, Nina Hertoghs, Andrew Raappana, Hayley Cardamone, Brian G. Oliver, Olesya Trakhimets, Sudhir Kumar, Nicholas Dambrauskas, Silvia A. Arredondo (+3 others)
2021 bioRxiv   pre-print
In our in vivo Py inhibition studies, the combination of mAbs brought the total antibody dose to four times that of the anti-PyCSP antibody alone (100 μg/mouse of anti-PyCSP alone to 400 μg/mouse total  ...  The interventions that have provided much of the previous progress, such as insecticidetreated bednets and large scale treatment programs, are highly susceptible to interruptions due to political or economic  ... 
doi:10.1101/2021.10.15.464611 fatcat:vpjeivmgebewpkxqro57du7aw4

Plasmodium Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole

Iset Medina Vera, Wandy L. Beatty, Photini Sinnis, Kami Kim, Kirk Deitsch
2011 PLoS Pathogens  
Scale bars are equal to 0.5 mm.  ...  Scale bars are equal to 0.5 mm. Abbreviations: PVM-Parasitophorous vacuole membrane, PV-Parasitophorous vacuole.  ... 
doi:10.1371/journal.ppat.1002197 pmid:21909259 pmcid:PMC3164628 fatcat:jgily2x4v5f3va4kzbv3sxdyau
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