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Otto, James H., and Blanc, Sam S. Biology Investigations. New York: Henry Holt and Company, 1951. 237 p

1952 Science Education  
., Anp Bianc, Sam S. Biology Investigations. New York: Henry Holt and Company, 1951. 237 p. This is a revision of the 1949 edition which was reviewed in the February, 1951, Science Educa- tion.  ... 
doi:10.1002/sce.3730360129 fatcat:ra7cetsf6rhavadoenmr3t44xm

Otto, James H. and Blanc, Sam S. Biology investigations. New York: Henry Holt and Company, 1949. 245 p

1951 Science Education  
Orto, JAMEs H. anp BLAnc, SAM §S. Biology In- vestigations. New York: Henry Holt and Com- pany, 1949, 245 p. ‘This is a combined workbook and laboratory manual in high school biology.  ... 
doi:10.1002/sce.3730350143 fatcat:lmkbumxwofg55hvqgy3a43rnq4

Preoperative cardiopulmonary exercise testing in England – a national survey

Sam Huddart, Emily L Young, Rebecca-Lea Smith, Peter JE Holt, Pradeep K Prabhu
2013 Perioperative Medicine  
Cardiopulmonary exercise testing (CPET) has become well established in the preoperative assessment of patients presenting for major surgery in the United Kingdom. There is evidence supporting its use in riskstratifying patients prior to major high-risk surgical procedures. We set out to establish how CPET services in England have developed since the only survey on this subject was undertaken in 2008 (J Intensive Care Soc 2009, 10:275-278). Methods: Availability of preoperative CPET and contact
more » ... etails were collected via a telephone survey and email invites to complete the online survey were sent to all contacts. The survey was live during March and April 2011. Results: We received 123 (74%) responses from the 166 emails that were sent out. In total, 32% (53/166) of all adult anesthetic departments in England have access to preoperative CPET services and a further 4% (6) were in the process of setting up services. The number of departments offering preoperative CPET, including those in the process of setting up services, has risen from 42 in 2008 to 59 in 2011, a rise of over 40%. Only 61% of the clinics are run by anesthetists and 39% of clinics have trained cardiorespiratory technicians assisting in the performance of the test. Most of the clinics (55%) rely solely on a bicycle ergometer. Vascular surgical patients are the largest group of patients tested, and the majority of tests are run to a symptom-limited maximum. We estimate that 15,000 tests are performed annually for preoperative assessment in England. Only 37% of respondents were confident that the tests performed were being billed for. Conclusions: CPET is increasing in popularity as a preoperative risk assessment tool. There remains a lack of consistency in the way tests are reported and utilized. The results highlight the extent and diversity of the use of preoperative CPET and the potential for further research into its use in unstudied patient groups.
doi:10.1186/2047-0525-2-4 pmid:24472647 pmcid:PMC3964325 fatcat:hlbakzej6fcgtkvly4bata73uq

A Parametric Study and Simulations in Quantifying Human Skin Hyperelastic Parameters

Jamaluddin Mahmud, Cathy Holt, Sam Evans, Nor Fazli Adull Manan, Mahmoud Chizari
2012 Procedia Engineering  
Case Study 1: μ = 10 Pa, α = 110 This is the initial study where the material parameters (μ = 10 Pa, α = 26) are used as proposed by Evans and Holt [17] .  ...  These initial values were selected based on the findings of Evans and Holt [17] when they measured skin properties using the DIC technique and FE modelling (μ = 10 Pa, α = 26).  ... 
doi:10.1016/j.proeng.2012.07.353 fatcat:3k5f6bqtkbefxlzth3bdrf4pam

Microstructure formation in radially counterstreaming electron flows

Lucas Ivan Inigo Gamiz, Bernhard Ersfeld, Enrico Brunetti, Sam Yoffe, R A Cairns, Adam Noble, George K Holt, Dino A Jaroszynski
2021 New Journal of Physics  
https://orcid.org/0000-0001-5597-9429 Samuel R Yoffe https://orcid.org/0000-0002-6723-4990 R Alan Cairns https://orcid.org/0000-0002-7562-2053 Adam Noble https://orcid.org/0000-0002-8181-7076 George K Holt  ... 
doi:10.1088/1367-2630/abe8f6 fatcat:ogqwxk5dengavokxe7l5u5vqbu

Repeated measures of PAP at different elevations in beef bulls in Colorado1,2

Taylor R Zimprich, Scott E Speidel, David W Schafer, Beth Lashell, Timothy N Holt, R Mark Enns, Sam F Cunningham, Milton G Thomas
2020 Translational Animal Science  
Historically, weaning PAP has been considered to be less accurate than yearling PAP (Holt and Callan, 2007; Zeng et al., 2016) ; this study also supports these findings.  ... 
doi:10.1093/tas/txaa116 pmid:33381732 pmcid:PMC7754211 fatcat:z2533iwd4fbmva4s3kkbvtaiua

Functional Expression of Exogenous Proteins in Mammalian Sensory Hair Cells Infected With Adenoviral Vectors

Jeffrey R. Holt, David C. Johns, Sam Wang, Zheng-Yi Chen, Robert J. Dunn, Eduardo Marban, David P. Corey
1999 Journal of Neurophysiology  
Holt and S. Wang are Associates at the Howard Hughes Medical Institute.  ...  et al. 1997 (Holt et al. , 1998 .  ...  Recording We recorded voltage-dependent and transduction currents, as previously described (Holt et al. 1997 (Holt et al. , 1998 .  ... 
doi:10.1152/jn.1999.81.4.1881 pmid:10200223 fatcat:rvaus5qarbftlb5e43xs6t62wu

β-Catenin maintains lung epithelial progenitors after lung specification

Edwin J. Ostrin, Danielle R. Little, Kamryn N. Gerner-Mauro, Elizabeth A. Sumner, Ricardo Ríos-Corzo, Elizabeth Ambrosio, Samantha E. Holt, Nicolas Forcioli-Conti, Haruhiko Akiyama, Sam M. Hanash, Shioko Kimura, Sarah X. L Huang (+1 others)
2018 Development  
The entire lung epithelium arises from SRY box 9 (SOX9)-expressing progenitors that form the respiratory tree and differentiate into airway and alveolar cells. Despite progress in understanding their initial specification within the embryonic foregut, how these progenitors are subsequently maintained is less clear. Using inducible, progenitorspecific genetic mosaic mouse models, we showed that β-catenin (CTNNB1) maintains lung progenitors by promoting a hierarchical lung progenitor gene
more » ... e, suppressing gastrointestinal (GI) genes, and regulating NK2 homeobox 1 (NKX2.1) and SRY box 2 (SOX2) in a developmental stage-dependent manner. At the early, but not later, stage post-lung specification, CTNNB1 cell-autonomously maintained normal NKX2.1 expression levels and suppressed ectopic SOX2 expression. Genetic epistasis analyses revealed that CTNNB1 is required for fibroblast growth factor (Fgf)/Kirsten rat sarcoma viral oncogene homolog (Kras)-mediated promotion of the progenitors. In silico screening of Eurexpress and translating ribosome affinity purification (TRAP)-RNAseq identified a progenitor gene signature, a subset of which depends on CTNNB1. Wnt signaling also maintained NKX2.1 expression and suppressed GI genes in cultured human lung progenitors derived from embryonic stem cells.
doi:10.1242/dev.160788 pmid:29440304 fatcat:jp7fkxph2bfdnklzvhlidoyyyi

A double-blind randomised placebo-controlled trial investigating the effects of lesogaberan on the objective cough frequency and capsaicin-evoked coughs in patients with refractory chronic cough

Huda Badri, Carmen Gibbard, Dimitra Denton, Imran Satia, Bashar Al-Sheklly, Rachel J. Dockry, Kimberley Holt, Kevin McGuiness, Sam Treadway, Peter Whorwell, Lesley Houghton, Augustine Lee (+6 others)
2022 ERJ Open Research  
Holt, L. Houghton and B.J. Canning: study concept; H. Badri, J.A.  ... 
doi:10.1183/23120541.00546-2021 pmid:35295236 pmcid:PMC8918934 fatcat:643dqkqiu5dz7oisq3yb53l42a

Fluoroquinolone-Mediated Inhibition of Cell Growth, S-G2/M Cell Cycle Arrest, and Apoptosis in Canine Osteosarcoma Cell Lines

Kyoung won Seo, Roseline Holt, Yong-Sam Jung, Carlos O. Rodriguez, Xinbin Chen, Robert B. Rebhun, Joseph Alan Bauer
2012 PLoS ONE  
Despite significant advancements in osteosarcoma research, the overall survival of canine and human osteosarcoma patients has remained essentially static over the past 2 decades. Post-operative limb-spare infection has been associated with improved survival in both species, yet a mechanism for improved survival has not been clearly established. Given that the majority of canine osteosarcoma patients experiencing post-operative infections were treated with fluoroquinolone antibiotics, we
more » ... ized that fluoroquinolone antibiotics might directly inhibit the survival and proliferation of canine osteosarcoma cells. Ciprofloxacin or enrofloxacin were found to inhibit p21 WAF1 expression resulting in decreased proliferation and increased S-G 2 /M accumulation. Furthermore, fluoroquinolone exposure induced apoptosis of canine osteosarcoma cells as demonstrated by cleavage of caspase-3 and PARP, and activation of caspase-3/7. These results support further studies examining the potential impact of quinolones on survival and proliferation of osteosarcoma.
doi:10.1371/journal.pone.0042960 pmid:22927942 pmcid:PMC3424257 fatcat:fcpueflpx5anxik4q4chst4yci

Testing the waters: Ethical considerations for including PrEP in a phase IIb HIV vaccine efficacy trial

Liza Dawson, Sam Garner, Chuka Anude, Paul Ndebele, Shelly Karuna, Renee Holt, Gail Broder, Jessica Handibode, Scott M Hammer, Magdalena E Sobieszczyk
2015 Clinical Trials  
The field of HIV prevention research has recently experienced some mixed results in efficacy trials of pre-exposure prophylaxis, vaginal microbicides, and HIV vaccines. While there have been positive trial results in some studies, in the near term, no single method will be sufficient to quell the epidemic. Improved HIV prevention methods, choices among methods, and coverage for all at-risk populations will be needed. The emergence of partially effective prevention methods that are not uniformly
more » ... available raises complex ethical and scientific questions regarding the design of ongoing prevention trials. Methods: We present here an ethical analysis regarding inclusion of pre-exposure prophylaxis in an ongoing phase IIb vaccine efficacy trial, HVTN 505. This is the first large vaccine efficacy trial to address the issue of pre-exposure prophylaxis, and the decisions made by the protocol team were informed by extensive stakeholder consultations. The key ethical concerns are analyzed here, and the process of stakeholder engagement and decision-making described. Discussion: This discussion and analysis will be useful as current and future research teams grapple with ethical and scientific study design questions emerging with the rapidly expanding evidence base for HIV prevention. Background The field of HIV prevention research has recently experienced some mixed results. Several biomedical interventions, including pre-exposure prophylaxis (PrEP), 1 treatment as prevention, 2 vaccines, 3 and vaginal microbicides, 4 have shown some success in clinical trials; however, negative trial results have complicated the picture. In the near term, no single method will be sufficient to quell the epidemic. Improved HIV prevention methods, choices among methods, and coverage for all at-risk populations will be needed. As biomedical interventions are tested and approved by regulatory bodies, questions arise as to whether they should be incorporated into ongoing prevention trials, raising complex scientific and ethical questions. We present here an ethical analysis regarding inclusion of PrEP in an ongoing vaccine efficacy trial, HVTN 505. This is the first large vaccine efficacy trial to address the issue of PrEP, and the decisions made by the protocol team were informed by extensive stakeholder consultations. The key ethical concerns are analyzed here, and the process of stakeholder engagement and decision-making described. This discussion and analysis will be useful as current and future research teams grapple with the rapidly expanding evidence base for HIV prevention.
doi:10.1177/1740774515579165 pmid:25851992 pmcid:PMC4506251 fatcat:4gyjrcxsnfawjjouu5ux6ld27a

The European Nucleotide Archive in 2018

Peter W Harrison, Blaise Alako, Clara Amid, Ana Cerdeño-Tárraga, Iain Cleland, Sam Holt, Abdulrahman Hussein, Suran Jayathilaka, Simon Kay, Thomas Keane, Rasko Leinonen, Xin Liu (+12 others)
2018 Nucleic Acids Research  
The European Nucleotide Archive (ENA; https://www. ebi.ac.uk/ena), provided from EMBL-EBI, has for more than three decades been responsible for archiving the world's public sequencing data and presenting this important resource to the scientific community to support and accelerate the global research effort. Here, we outline ENA services and content in 2018 and provide an overview of a selection of focus areas of development work: extending data coordination services around ENA, sequence
more » ... ions through template expansion, early pre-submission validation tools and our move towards a new browser and retrieval infrastructure.
doi:10.1093/nar/gky1078 pmid:30395270 fatcat:kmr4vyojundfzabhv3dwdciamu

Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin

Matthew T. Holt, Yael David, Sam Pollock, Zhanyun Tang, Jongcheol Jeon, Jaehoon Kim, Robert G. Roeder, Tom W. Muir
2015 Proceedings of the National Academy of Sciences of the United States of America  
| www.pnas.org/cgi/doi/10.1073/pnas.1504483112 Holt et al. | www.pnas.org/cgi/doi/10.1073/pnas.1504483112 Holt et al. | www.pnas.org/cgi/doi/10.1073/pnas.1504483112 Holt et al.  ...  (A) 3 H-SAM hDot1L methyltransferase assays using unmodified (lane 1), H2B-Ub (lane 2), and H2B-uLL (lane 3) nucleosomes.  ... 
doi:10.1073/pnas.1504483112 pmid:26240340 pmcid:PMC4547310 fatcat:ahdvc563vrczxgvrgfjll5aemi

Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model

Chloe F. Moss, Ilaria Dalla Rosa, Lilian E. Hunt, Takehiro Yasukawa, Robert Young, Aleck W. E. Jones, Kaalak Reddy, Radha Desai, Sam Virtue, Greg Elgar, Peter Voshol, Martin S. Taylor (+3 others)
2017 Nucleic Acids Research  
The mapped read (sam) files were then converted to bam format using samtools version 0.1.19 (18) . The reads in each bam file were sorted and indexed using samtools.  ... 
doi:10.1093/nar/gkx1009 pmid:29106596 pmcid:PMC5728394 fatcat:6rmbtrjiq5d2jp7sjyl5ntf3wu

sj-pdf-1-jba-10.1177_0885328220983797 - Supplemental material for Alternative radiopacifiers for polymethyl methacrylate bone cements: Silane-treated anatase titanium dioxide and yttria-stabilised zirconium dioxide

Wayne Nishio Ayre, Nicole Scully, Carole Elford, Bronwen AJ Evans, Wendy Rowe, Jeff Rowlands, Ravi Mitha, Paul Malpas, Panagiota Manti, Cathy Holt, Rhidian Morgan-Jones, James C Birchall (+2 others)
2021 Figshare  
, Rhidian Morgan-Jones, James C Birchall, Stephen P Denyer and Sam L Evans in Journal of Biomaterials Applications  ...  titanium dioxide and yttria-stabilised zirconium dioxide by Wayne Nishio Ayre Nicole Scully, Carole Elford, Bronwen AJ Evans, Wendy Rowe, Jeff Rowlands, Ravi Mitha, Paul Malpas, Panagiota Manti, Cathy Holt  ... 
doi:10.25384/sage.14006094.v1 fatcat:iugddfngsjdnfl5va3cdxts7by
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