868 Hits in 4.9 sec

Vector filtering for color imaging

R. Lukac, B. Smolka, K. Martin, K.N. Plataniotis, A.N. Venetsanopoulos
2005 IEEE Signal Processing Magazine  
Figure 11 shows the results where the input is a television image. Finally, "Vector Processing of cDNA Microarray Images" shows results from applying the filters to a cDNA microarray image.  ...  On the other hand, a filter operating on a smaller spatial neighborhood can better match image details [2] .  ... 
doi:10.1109/msp.2005.1407717 fatcat:eiqfzdppl5fhngggglhblbiyjm

DNA Microarrays: a Powerful Genomic Tool for Biomedical and Clinical Research

Victor Trevino, Francesco Falciani, Hugo A. Barrera-Saldaña
2007 Molecular Medicine  
ACKNOWLEDGMENTS HABS thanks the Staff of the Microarray Technology EMBO-INER Advanced Practical Course for enjoyable course lessons, materials and results; Peter Davies, Nancy and Greg Shipley of UT Medical  ...  The digital image is further processed by specialized software to transform the image of each spot to a numerical reading.  ...  Step 2: Microarray Scanning, Spot Finding and Image Processing Microarrays were scanned using Sca-nArray Express (PerkinElmer, Waltham, MA, USA).  ... 
doi:10.2119/2006-00107.trevino pmid:17660860 pmcid:PMC1933257 fatcat:5wd6d6w67bapfl33lkcbisuu3a

Gene Expression-Assisted Cancer Prediction Techniques

Tanima Thakur, Isha Batra, Monica Luthra, Shanmuganathan Vimal, Gaurav Dhiman, Arun Malik, Mohammad Shabaz, Dmitry Zaitsev
2021 Journal of Healthcare Engineering  
Gene expression is a process that is used to convert deoxyribose nucleic acid (DNA) to ribose nucleic acid (RNA) and then RNA to protein.  ...  This paper will present a review of some of those techniques from the literature; details about the various datasets on which these techniques are implemented and the advantages and disadvantages.  ...  hand-held devices.  ... 
doi:10.1155/2021/4242646 pmid:34545300 pmcid:PMC8449724 fatcat:6qh3yl3dkjhipiq46v3c2cmq5m

Medical applications of microarray technologies: a regulatory science perspective

Emanuel F. Petricoin, Joseph L. Hackett, Lawrence J. Lesko, Raj K. Puri, Steven I. Gutman, Konstantin Chumakov, Janet Woodcock, David W. Feigal, Kathryn C. Zoon, Frank D. Sistare
2002 Nature Genetics  
The development of a cooperative framework among regulators, product sponsors, and technology experts will be essential for realizing the revolutionary promise that microarrays hold for drug development  ...  The potential medical applications of microarrays have generated much excitement, and some skepticism, within the biomedical community.  ...  But on the other hand, misleading information could introduce chaos into a time-tested system that assures consumers that pharmaceuticals, biologics and devices provided by trained health professionals  ... 
doi:10.1038/ng1029 pmid:12454641 fatcat:zu5ibv3cr5hwtguintdtu643g4

Microfluidics-based extraction of viral RNA from infected mammalian cells for disposable molecular diagnostics

Arpita Bhattacharyya, Catherine M. Klapperich
2008 Sensors and actuators. B, Chemical  
The device offers a robust, easy-to-use sample preparation platform for rapid processing of clinical samples for nucleic acid-based diagnostics for pathogens.  ...  The SPE system also significantly reduces the required hands-on time for extraction compared to the conventional bench-top extraction procedures.  ...  The sample preparation in the device was performed with a silica bead packed column held in place with an etched weir.  ... 
doi:10.1016/j.snb.2007.09.057 fatcat:bcobzo6hlvhqlg3pglwg4wmvqm

High throughput analysis reveals dissociable gene expression profiles in two independent neural systems involved in the regulation of social behavior

Tyler J Stevenson, Kirstin Replogle, Jenny Drnevich, David F Clayton, Gregory F Ball
2012 BMC Neuroscience  
Results: We show using microarray cDNA analysis that two discrete brain systems that are both involved in governing singing behavior show markedly different gene expression profiles.  ...  epigenetic and angiogenetic processes.  ...  RA on the other hand exhibits different gene expression profiles across the reproductive states; some of the cDNAs follow the categorical switch between LD7 and LD21 but many others have already changed  ... 
doi:10.1186/1471-2202-13-126 pmid:23078552 pmcid:PMC3485171 fatcat:clzm6yfwsjddxhq4nyhhpkeh7y

New DNA Sequencing Methods

Andre Marziali, Mark Akeson
2001 Annual Review of Biomedical Engineering  
When the DNA was closely packed, high-resolution images revealed a right-handed double helix with the expected pitch of 3.4 nm.  ...  It combines the highly parallel nature of expression microarrays with the statistically robust digital output from gel-based sequencing of clones from representative cDNA libraries (62) .  ... 
doi:10.1146/annurev.bioeng.3.1.195 pmid:11447062 fatcat:63ohbirdrze67dkvzpzmd52xke

Lab-on-a-Chip, Micro- and Nanoscale Immunoassay Systems, and Microarrays [chapter]

Simon Rattle, Oliver Hofmann, Christopher P. Price, Larry J. Kricka, David Wild
2013 The Immunoassay Handbook  
As an important step toward POC applications, Davis and coworkers have developed a fully self-contained hand-held SPR device powered by a 9 V battery (Feltis et al., 2008) .  ...  On the other hand, lateral flow-based devices are easy to use, low cost, and stand-alone but lack precise quantification capabilities.  ... 
doi:10.1016/b978-0-08-097037-0.00013-0 fatcat:qyn2jgeucbfudeym4opk2fwr7y


Roland B. Stoughton
2005 Annual Review of Biochemistry  
Setting up a robust complete microarray experiment process by assembling individual components is a challenge.  ...  These held bacterial colonies carrying different cosmid genomic inserts and later held preidentified cDNAs, with spacings down to ∼2 mm.  ... 
doi:10.1146/annurev.biochem.74.082803.133212 pmid:15952881 fatcat:ltohntflwvb5hplf5pktzpg5e4

Radiation dose-rate effects on gene expression for human biodosimetry

Shanaz A. Ghandhi, Lubomir B. Smilenov, Carl D. Elliston, Mashkura Chowdhury, Sally A. Amundson
2015 BMC Medical Genomics  
A support vectors machine classifier was able to distinguish between dose-rates with 100 % accuracy using leave-one-out cross-validation.  ...  Gene ontology terms enriched in both sets of genes were related to immune processes and B-cell mediated immunity.  ...  directly by numeric readouts from a GMP70 Hand-held CO 2 meter (Vaisala, Finland).  ... 
doi:10.1186/s12920-015-0097-x pmid:25963628 pmcid:PMC4472181 fatcat:dh5rhwclfzcv5cdj5vksurrgfa

RhNAC2 and RhEXPA4 Are Involved in the Regulation of Dehydration Tolerance during the Expansion of Rose Petals

F. Dai, C. Zhang, X. Jiang, M. Kang, X. Yin, P. Lu, X. Zhang, Y. Zheng, J. Gao
2012 Plant Physiology  
Cy3 and Cy5 probes were mixed in equal quantities and purified on spin columns (Minipore) to a final volume of  ...  The petal RNA samples were used to generate Cy3-and Cy5-labeled cDNA probes using the SuperScript direct cDNA Labeling System (Invitrogen) following the manufacturer's instructions.  ...  amino-silaned glass slides to make a cDNA microarray.  ... 
doi:10.1104/pp.112.207720 pmid:23093360 pmcid:PMC3510132 fatcat:h6hibkw3ibhfflijvmkerxm53q

Transcriptional Profiling of C. albicans in a Two Species Biofilm with Rothia dentocariosa

Priya Uppuluri, Henk J. Busscher, Jaideep Chakladar, Henny C. van der Mei, W. LaJean Chaffin
2017 Frontiers in Cellular and Infection Microbiology  
Biofilms on silicone rubber voice prostheses are the major cause for frequent failure and replacement of these devices.  ...  An in vitro biofilm comprising these two organisms was developed on silicone rubber, a material used for Groningen button voice prosthesis.  ...  The images were processed for display using Photoshop software (Adobe, Mountain View, Calif.).  ... 
doi:10.3389/fcimb.2017.00311 pmid:28752078 pmcid:PMC5508013 fatcat:4nietfqdw5aijbgbocnqwqgcvu

Gene expression profiles of human breast cancer progression

X.-J. Ma, R. Salunga, J. T. Tuggle, J. Gaudet, E. Enright, P. McQuary, T. Payette, M. Pistone, K. Stecker, B. M. Zhang, Y.-X. Zhou, H. Varnholt (+7 others)
2003 Proceedings of the National Academy of Sciences of the United States of America  
Furthermore, a subset of genes associated with high tumor grade is quantitatively correlated with the transition from preinvasive to invasive growth.  ...  Here, through the combined use of laser capture microdissection and DNA microarrays, we have generated in situ gene expression profiles of the premalignant, preinvasive, and invasive stages of human breast  ...  , and a grant from the Avon Foundation (to D.C.S.).  ... 
doi:10.1073/pnas.0931261100 pmid:12714683 pmcid:PMC156311 fatcat:kbxwsdv45vbfzpauyxjpu3aqci

Genome-Wide Gene Expression in relation to Age in Large Laboratory Cohorts ofDrosophila melanogaster

Kimberly A. Carlson, Kylee Gardner, Anjeza Pashaj, Darby J. Carlson, Fang Yu, James D. Eudy, Chi Zhang, Lawrence G. Harshman
2015 Genetics Research International  
Aging is a complex process characterized by a steady decline in an organism's ability to perform life-sustaining tasks.  ...  A linear model for microarray data method was used for the microarray analysis to adjust for the box effect; it identified 1,581 candidate aging genes.  ...  Genetics Research International Acknowledgments The authors thank Tiffany Schwasinger for collecting the flies, Jeff Kittrell for microarray data banking assistance, and Dr.  ... 
doi:10.1155/2015/835624 pmid:26090231 pmcid:PMC4454753 fatcat:2ftymfoa65eancz2itg36uggce

Tension stimulation drives tissue formation in scaffold-free systems

Jennifer K. Lee, Le W. Huwe, Nikolaos Paschos, Ashkan Aryaei, Courtney A. Gegg, Jerry C. Hu, Kyriacos A. Athanasiou
2017 Nature Materials  
Here, by using self-assembled articular cartilage as a model to examine the e ects of intermittent and continuous tension stimulation on tissue formation, we show that the application of tension alone,  ...  Tension stimulation, which results in nearly sixfold improvements in tensile properties over unstimulated controls, may allow the engineering of mechanically robust biological replacements of native tissue  ...  Cassereau and the Weaver laboratory for assistance with second harmonic generation imaging.  ... 
doi:10.1038/nmat4917 pmid:28604717 pmcid:PMC5532069 fatcat:gpnkmo4wgzcodcyyiounxv43za
« Previous Showing results 1 — 15 out of 868 results