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Replication Pauses of the Wild-Type and Mutant Mitochondrial DNA Polymerase Gamma: A Simulation Study
2011
PLoS Computational Biology
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Pausing of pol-c might increase the chance of deletion and depletion of mitochondrial DNA. ...
A467T variant showed at most a doubling in the length of the pauses compared to the wild-type. ...
Results We modeled three types of pol c, the wild type and two mutants (the human pathogenic variation A467T and the completely
Author Summary As a polymerase copies a DNA strand, it may briefly pause ...
doi:10.1371/journal.pcbi.1002287
pmid:22125488
pmcid:PMC3219627
fatcat:s3lbj6xuwjanvbdom5bfubfjii
Analysis of mutational spectra by denaturing capillary electrophoresis
2008
Nature Protocols
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DCE has been used to define quantitative point mutational spectra for study a wide variety of genetic phenomena: errors of DNA polymerases, mutations induced in human cells by chemicals and irradiation ...
Numbers and kinds of point mutant within DNA from cells, tissues and human population may be discovered for nearly any 75-250bp DNA sequence. ...
Wild type DNA was amplified with Taq DNA polymerase, which has been shown to induce mutants at about 10 −5 /base pair/ doubling 49 . ...
doi:10.1038/nprot.2008.79
pmid:18600220
pmcid:PMC2742298
fatcat:avvvcsekujep7kabj4zdel3rcm
General sessions (1A-01 – 3E-11)
2012
Genes & Genetic Systems
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Buckwheat exhibits self-incompatibility and breeding between individuals with the same type of flowers fails. ...
This heterostylous self-incompatible trait is governed by a single genetic locus or gene complex, i.e., S-locus, and the genotypes of plants with short and long style flowers are S/s and s/s, respectively ...
The one of the PolB-like proteins from C. subterraneum indeed behaves like a typical B-family DNA polymerase, i.e. seems to be primarily a DNA replication polymerase, based on its processivity rates and ...
doi:10.1266/ggs.87.397
fatcat:ab6vtx5e2ncdjlggsn4nguxyha
EUROMIT 6
2004
Biochimica et Biophysica Acta - Bioenergetics
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Genetic studies reveal mutations in the mitochondrial DNA-dependent DNA polymerase gamma (POLG) gene. ...
POLG1 encodes the catalytic alpha subunit of polymerase gamma and is the only polymerase known to be involved in mtDNA replication. ...
the type I error. ...
doi:10.1016/j.bbabio.2004.05.001
fatcat:ocvsmh3qvfg6xkhvkr64vfzkfe
DNA polymerases: Mechanistic insight from biochemical and biophysical studies
2012
Frontiers in Bioscience
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Replicative and repair DNA polymerases and 8-oxoguanine lesion 4. Nucleotide incorporation reaction of TLS DNA polymerases 4.1. Domain organization of the Y family of DNA polymerases 4.2. ...
References ABSTRACT In vivo the DNA polymerases are responsible for replicative and repair DNA synthesis. ...
Introducing the notion of dynamic processivity Recent measurements of DNA polymerase processivity using wild type and specific mutant DNA polymerases in the context of a full replisome led us to revisit ...
doi:10.2741/3941
pmid:22201758
fatcat:3oyz33bpnnbola5fovs5ab4rxu
Cellular responses to environmental DNA damage
1991
Mutation Research/DNA Repair
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mutant population and DGGE was used to separate wild type DNA sequences from mutant sequences. ...
In past studies, when an amber mutation at position 128 was suppressed with the incorporation of a serine residue, the resulting mutant enzyme had wild type D NA binding and normal glycosylic bond scisoion ...
Ml3mp2 single-stranded DNA was used as a template for in vitro DNA synthesis by wild-type and the above two The replicated molecule (RF) was introduced into the competent cells of E. coil $90C (routs + ...
doi:10.1016/0921-8777(91)90055-t
fatcat:wbtywgadgfbydbto7zbsybdqgu
The Universally Conserved Prokaryotic GTPases
2011
Microbiology and Molecular Biology Reviews
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Moreover, E. coli obg mutant cells have a higher DNA content than do wild-type cells, and Obg was suggested previously to regulate the total DNA content within E. coli cells (202) . ...
A first observation hinting at a possible role for Obg in DNA replication came from studies with a heat-sensitive B. subtilis obg mutant. ...
A C. glutamicum ⌬hflX strain has the same cell morphology and growth behavior as those of the wild type (78) . Role in ribosome assembly. ...
doi:10.1128/mmbr.00009-11
pmid:21885683
pmcid:PMC3165542
fatcat:lgjrr5v3izbstdnktcbnzzzebe
Dissecting Highly Mutagenic Processing of Complex Clustered DNA Damage in Yeast Saccharomyces cerevisiae
2021
Cells
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in wild-type, BER, and translesion synthesis-deficient cells. ...
Clusters of DNA damage, also called multiply damaged sites (MDS), are a signature of ionizing radiation exposure. ...
E.S. is very thankful to late Betsy Sutherland, to Lynn Harrison, and Susan Wallace for their warm encouragement and support for our studies on the processing of clustered DNA damage and their outcome. ...
doi:10.3390/cells10092309
pmid:34571958
pmcid:PMC8471780
fatcat:vquktuys7vevpk25mg7l2dgpji
EBEC Short Reports Volume 13
2004
Biochimica et Biophysica Acta - Bioenergetics
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The cytochrome c machinery in B. subtilis is of the so called System II type which is also present in plants. ...
We have found that a wide range of different structures, only vaguely resembling fatty acids, are able to initiate UCP1-dependent (re)activation of mitochondrial thermogenesis. ...
Both single mutants have 50% of wild-type ATPase activity. The reduced double mutant has 30% of wild-type ATPase activity. ...
doi:10.1016/j.bbabio.2004.06.002
fatcat:kwzzktmrzbfnlk54eebael35rm
Correlating Transcription Initiation and Conformational Changes by a Single-Subunit RNA Polymerase with Near Base-Pair Resolution
2018
Molecular Cell
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We provide a comprehensive analysis of transcription in real-time by T7 RNA Polymerase (RNAP) using single-molecule fluorescence resonance energy transfer by monitoring the entire life history of transcription ...
They distinguish productive and failed transcription, and show that a majority of productive transcription occurs without abortive initiation, and most of the abortive RNAs result from multiple rounds ...
Acknowledgments This work was supported by NIH grants (GM118086, GM 122569) and by the Korea Research Foundation Grant (KRF-2006-612-C0020). ...
doi:10.1016/j.molcel.2018.04.018
pmid:29775583
pmcid:PMC5983381
fatcat:xaheo7r6szcehn2hbd7e732t7a
1 Keynote address by Nobel Laureate Phillip A. Sharp the 21st century opened with a revolution in RNA biology
2015
Journal of Biomolecular Structure and Dynamics
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The discoveries of RNA interference and the evolutionary conservation of microRNAs at the turn of the century heralded a revolution in RNA Biology and the development of important innovations. ...
Long non-coding RNAs regulate the expression of genes through both cis and trans mechanisms and provide organization to the nucleus. ...
PNA binding to the non-template DNA strand interferes with transcription, suggesting a blockage mechanism mediated by R-loop formation. Molecular Carcinogenesis. (Epub ahead of print). ...
doi:10.1080/07391102.2015.1032624
fatcat:w7iil7k3ize77bjkthcyi52ohy
Meeting report: 32nd International Conference on Antiviral Research
2019
Antiviral Research
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It provides a general review of the meeting and awardees, summarizing the presentations, and their main conclusions from the perspective of researchers active in many different areas of antiviral research ...
This report gives an overview of the conference on behalf of the Society. ...
Acknowledgements We thank all of the speakers, presenters, participants, organizers, and sponsors of the 32nd ICAR for their contributions to such a successful meeting. ...
doi:10.1016/j.antiviral.2019.104550
pmid:31302149
pmcid:PMC7105345
fatcat:27w3m6jfybghdlrrkanjlhzzpy
Abstracts of the 29th Annual Symposium of The Protein Society
2015
Protein Science
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Nuclear magnetic resonance (NMR) is a powerful biophysical method for studying protein-ligand interactions in solution and elucidating the mechanism of action of potential inhibitors. ...
Finally, 19F-NMR was used to study solvent exposure of the complex and its dynamics in solution through relaxation dispersion of the 19F-nucleus at different temperatures. ...
Inside the membrane, the relaxosome identifies the single strand for transfer in a plasmid DNA, relaxes and unwinds it, whereas the transferosome is involved in pilus formation (Type IV secretion system ...
doi:10.1002/pro.2823
pmid:26452528
pmcid:PMC4632850
fatcat:ejmksoqj3zbsrkabl4jyp7emgi
The 28th Annual Symposium of The Protein Society: Program & Abstracts
2014
Protein Science
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DNA replication system in the yeast Saccharomyces cerevisiae. 1 This system consists of an orthogonal DNA plasmid-DNA polymerase pair wherein the orthogonal DNA polymerase stably and specifically replicates ...
Computational models and experiments differentiated the structure and behaviour of the wild type from the mutant -differences which may underpin the impaired mechanosensing ability of the mutant found ...
The catalytic and three-dimensional differences between al the analyzed mutants are reported, and their roll in the dimeric wild type nature of this enzyme discussed. ...
doi:10.1002/pro.2504
pmid:25043257
pmcid:PMC4116645
fatcat:yjhug3aif5futn3icovvrkgebm
The effects of extremely low frequency magnetic fields on mutation induction in mice
2015
Mutation research
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I would like to take this opportunity to express my utmost gratitude to my supervisor Professor Yuri Dubrova, for all his advice, guidance, and support throughout my time in the laboratory and the completion ...
of this thesis. ...
It was therefore theorised that ESTR mutations arise through a replication or repair based process involving DNA polymerase slippage during DNA replication, or through DNA polymerase pausing to repair ...
doi:10.1016/j.mrfmmm.2015.01.014
pmid:25769183
fatcat:fhcdhlbiazghtervmdre4vubyy
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