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Molecular genetic approaches to studying fertilization in model systems
2004
Reproduction
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In a wide range of experimental systems, a variety of both forward and reverse genetic approaches are becoming available for the study of the molecules involved in fertilization. ...
We highlight some of the recent advances resulting from these genetic methods and their applications in these systems. ...
One of the first instances of the use of a genetic model system to study fertilization was with t-haplotype mice. ...
doi:10.1530/rep.1.00009
pmid:15047931
fatcat:5bzgi3pxkjg7phtluelqxjxviu
Ubiquitin-Proteasome System–Regulated Protein Degradation in Spermatogenesis
2022
Cells
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The ubiquitin proteasome system (UPS) plays central roles in the precise control of protein homeostasis to ensure the effectiveness of certain protein groups at a given stage and the inactivation of them ...
Exploring the functions of UPS machineries in spermatogenesis provides a global picture of the proteome dynamics during male germ cell production and shed light on the etiology and pathogenesis of human ...
Conflicts of Interest: The authors declare no competing interests. ...
doi:10.3390/cells11061058
pmid:35326509
pmcid:PMC8947704
fatcat:rkvijxvqnzf35klkatm5dkpo7e
Studies on mutation at esterase loci in Drosophila virilis. III. Genic variation in Est-.ALPHA. alleles produced by mutation experiments
1985
The Japanese Journal of Genetics
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With the highly polymorphic Est-a locus of Drosophila virilis, enquiry was made into its genetic structure. ...
, specific base sequence where recombination is unaccompanied with base correction of the unpaired bases. ...
The author thanks Drs. K. I. Sakai and L. D. Frye for their critical reading of the manuscript. This work was partly supported by a Grant (Nos. 64, 68, and 73) from Josai Dental University. ...
doi:10.1266/jjg.60.103
fatcat:kkfdhivdgfabnekalwdnrsaa2q
Type I Restriction Systems: Sophisticated Molecular Machines (a Legacy of Bertani and Weigle)
2000
Microbiology and Molecular Biology Reviews
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New specificities as the result of the reassortment of TRDs depend upon a natural reservoir of related hsdS genes avail-able for genetic recombination. ...
The effect of psoralen has not been reinvestigated using the refined techniques and substrates currently available; cross-links might trigger cutting if they impede the translocating complex. ...
doi:10.1128/mmbr.64.2.412-434.2000
pmid:10839821
pmcid:PMC98998
fatcat:ujkg3in6jvd43nfjmbajxzyvee
Reprogramming the genetic code in vitro
2014
TIBS -Trends in Biochemical Sciences. Regular ed
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Further, the 41 combination of genetic code reprogramming with selection-based techniques allows 42 the parallel screening of trillions of peptide-like molecules for pharmacological 43 activity or for ...
genetic reprogramming techniques developed to date can be broadly classified 54 into those techniques which involve genetic reprogramming in a living cell (Box 1) 55 and those that utilize in vitro translation ...
323 analogues of phenylalanine), and it was demonstrated that the resultant mutant EF-Tu 324 afforded the translation of such amino acids in a purified recombinant translation 325 system, whereas wild-type ...
doi:10.1016/j.tibs.2014.07.005
pmid:25129886
fatcat:hlq3vjtokfeujgpumtkmp6fclq
THEEVOLUTION OFASEXUALFUNGI: Reproduction, Speciation and Classification
1999
Annual Review of Phytopathology
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Knowing the identity of species and populations and their reproductive modes, while taking a broad view of pathogen behavior in space and time, should enhance the ability of pathologists to control pathogens ...
Phylogenetic and population genetic methods that compare nucleic acid variation are being used to identify species and populations of pathogenic fungi and determine how they reproduce in nature. ...
replacement to simulate the effects of recombination. ...
doi:10.1146/annurev.phyto.37.1.197
pmid:11701822
fatcat:cy36pqijy5hafj7vsqfuy6o5tu
Stabilization of microsatellite sequences by variant repeats in the yeast Saccharomyces cerevisiae
1997
Genetics
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We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT). ...
The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch repair genes pms1, msh2 and msh3, indicating that this effect does not require a functional DNA ...
In this article we reinvestigate the effects of variant repeats on the stability of the poly GT microsatellite. ...
pmid:9178000
pmcid:PMC1207991
fatcat:dtyjkgginve7bkqw3go7kp62nm
Genetic Divergence of Bradyrhizobium Strains Nodulating Soybeans as Revealed by Multilocus Sequence Analysis of Genes Inside and Outside the Symbiosis Island
2014
Applied and Environmental Microbiology
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The gene flow level of island genes among genospecies is discussed in the context of the divergence level of off-island genes. ...
However, seven genes inside the symbiosis island ("island" genes;nifA,nifH,nodC,nodV,fixA,trpD, andrhcC2) showed contrasting lower levels of nucleotide diversity and recombination rates than did off-island ...
This study was funded by the National Natural Science Foundation of China (31200002). ...
doi:10.1128/aem.00044-14
pmid:24632260
pmcid:PMC4018923
fatcat:zsyrtvw4kbfgtoesuwdu6r35ue
TheIn VitroRedundant Enzymes PurN and PurT Are Both Essential for Systemic Infection of Mice in Salmonella enterica Serovar Typhimurium
2016
Infection and Immunity
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The results of the present study call for reinvestigations of the concept of metabolic redundancy inS. Typhimuriumin vivo. ...
In the process of further characterization, we disclosed that the glycine cleavage system (GCV) was the most important for formation of C1unitsin vivo(CI = 0.03 ± 0.03). ...
ACKNOWLEDGMENTS The technical assistance of Tony Bønnelycke and Pia Mortensen is highly appreciated. ...
doi:10.1128/iai.00182-16
pmid:27113361
pmcid:PMC4936368
fatcat:g77dvrvm7baqravpbnbmmzaqva
SEASONAL VARIATIONS IN PHYTOPLANKTON COMMUNITIES OF AN EPHEMERAL POND SYSTEM IN SOUTHEASTERN VIRGINIA
2000
Journal of Phycology
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We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis. ...
Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 . ...
These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica. ...
doi:10.1046/j.1529-8817.1999.00001-111.x
fatcat:yjd6u43rv5eofassgcjpsbect4
Reverse Genetics for Type I Feline Coronavirus Field Isolate To Study the Molecular Pathogenesis of Feline Infectious Peritonitis
2018
mBio
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Here, we report the first reverse genetics system based on a serotype I FECV field isolate that is suitable to produce high-titer stocks of recombinant FECVs. ...
To our knowledge, we report the first serotype I FECV field isolate-based reverse genetics system that allows the production of high-titer recombinant virus stocks that can be used for subsequent in vivo ...
RESULTS Complete genome sequence of a serotype I FECV field isolate. In this study, we aimed to establish a reverse genetics system based on a serotype I FECV field isolate. ...
doi:10.1128/mbio.01422-18
pmid:30065095
pmcid:PMC6069117
fatcat:3cimpidqurfclhkgul7qpjbpnm
LIGHT HARVESTING ANTENNAE SYSTEMS: LESSONS FROM SIMPLE ALGAE, THE ROLE OF SCIENTISTS, AND TRENDS
2000
Journal of Phycology
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We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis. ...
Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 . ...
These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica. ...
doi:10.1046/j.1529-8817.1999.00001-69.x
fatcat:wg3bt4nsg5e7bp2coluezdzruy
SURVEY OF BENTHIC DIATOM COMMUNITIES FROM LOTIC SYSTEMS WITHIN THE WESTERN ALLEGHENY PLATEAU
2000
Journal of Phycology
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We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis. ...
Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 . ...
These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica. ...
doi:10.1046/j.1529-8817.1999.00001-201.x
fatcat:rjj7dbtzkvc7xafe2j24e52yry
MONITORING THE PERSISTENCE OF GIANT KELP AROUND SANTA CATALINA ISLAND USING A GEOGRAPHIC INFORMATION SYSTEM
2000
Journal of Phycology
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We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis. ...
Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 . ...
These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica. ...
doi:10.1046/j.1529-8817.1999.00001-27.x
fatcat:r47z3hmw4bcjlpy5edpg2rxo64
Species-Specific Pharmacological Properties of Human 2A-Adrenoceptors
2000
Hypertension
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The antagonistic property of rilmenidine at human ␣ 2A -adrenoceptors indicates that in contrast to the suggestion based on rabbit data, the hypotensive property of the drug in humans is not due to activation ...
In addition, a partial nucleotide and amino acid sequence of the rabbit ␣ 2A -adrenoceptor (a region known to substantially influence the pharmacological characteristics of the ␣ 2 -adrenoceptor) revealed ...
Acknowledgments This study was supported by a grant from the Deutsche Forschungsgemeinschaft. ...
doi:10.1161/01.hyp.36.3.405
pmid:10988273
fatcat:32dgj7pip5crrprscsoo6dlyly
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