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Molecular genetic approaches to studying fertilization in model systems

Brian Geldziler, Pavan Kadandale, Andrew Singson
2004 Reproduction  
In a wide range of experimental systems, a variety of both forward and reverse genetic approaches are becoming available for the study of the molecules involved in fertilization.  ...  We highlight some of the recent advances resulting from these genetic methods and their applications in these systems.  ...  One of the first instances of the use of a genetic model system to study fertilization was with t-haplotype mice.  ... 
doi:10.1530/rep.1.00009 pmid:15047931 fatcat:5bzgi3pxkjg7phtluelqxjxviu

Ubiquitin-Proteasome System–Regulated Protein Degradation in Spermatogenesis

Yi Xiong, Chao Yu, Qianting Zhang
2022 Cells  
The ubiquitin proteasome system (UPS) plays central roles in the precise control of protein homeostasis to ensure the effectiveness of certain protein groups at a given stage and the inactivation of them  ...  Exploring the functions of UPS machineries in spermatogenesis provides a global picture of the proteome dynamics during male germ cell production and shed light on the etiology and pathogenesis of human  ...  Conflicts of Interest: The authors declare no competing interests.  ... 
doi:10.3390/cells11061058 pmid:35326509 pmcid:PMC8947704 fatcat:rkvijxvqnzf35klkatm5dkpo7e

Studies on mutation at esterase loci in Drosophila virilis. III. Genic variation in Est-.ALPHA. alleles produced by mutation experiments

Kendo TSUNO
1985 The Japanese Journal of Genetics  
With the highly polymorphic Est-a locus of Drosophila virilis, enquiry was made into its genetic structure.  ...  , specific base sequence where recombination is unaccompanied with base correction of the unpaired bases.  ...  The author thanks Drs. K. I. Sakai and L. D. Frye for their critical reading of the manuscript. This work was partly supported by a Grant (Nos. 64, 68, and 73) from Josai Dental University.  ... 
doi:10.1266/jjg.60.103 fatcat:kkfdhivdgfabnekalwdnrsaa2q

Type I Restriction Systems: Sophisticated Molecular Machines (a Legacy of Bertani and Weigle)

N. E. Murray
2000 Microbiology and Molecular Biology Reviews  
New specificities as the result of the reassortment of TRDs depend upon a natural reservoir of related hsdS genes avail-able for genetic recombination.  ...  The effect of psoralen has not been reinvestigated using the refined techniques and substrates currently available; cross-links might trigger cutting if they impede the translocating complex.  ... 
doi:10.1128/mmbr.64.2.412-434.2000 pmid:10839821 pmcid:PMC98998 fatcat:ujkg3in6jvd43nfjmbajxzyvee

Reprogramming the genetic code in vitro

Toby Passioura, Hiroaki Suga
2014 TIBS -Trends in Biochemical Sciences. Regular ed  
Further, the 41 combination of genetic code reprogramming with selection-based techniques allows 42 the parallel screening of trillions of peptide-like molecules for pharmacological 43 activity or for  ...  genetic reprogramming techniques developed to date can be broadly classified 54 into those techniques which involve genetic reprogramming in a living cell (Box 1) 55 and those that utilize in vitro translation  ...  323 analogues of phenylalanine), and it was demonstrated that the resultant mutant EF-Tu 324 afforded the translation of such amino acids in a purified recombinant translation 325 system, whereas wild-type  ... 
doi:10.1016/j.tibs.2014.07.005 pmid:25129886 fatcat:hlq3vjtokfeujgpumtkmp6fclq

THEEVOLUTION OFASEXUALFUNGI: Reproduction, Speciation and Classification

JW Taylor, DJ Jacobson, MC Fisher
1999 Annual Review of Phytopathology  
Knowing the identity of species and populations and their reproductive modes, while taking a broad view of pathogen behavior in space and time, should enhance the ability of pathologists to control pathogens  ...  Phylogenetic and population genetic methods that compare nucleic acid variation are being used to identify species and populations of pathogenic fungi and determine how they reproduce in nature.  ...  replacement to simulate the effects of recombination.  ... 
doi:10.1146/annurev.phyto.37.1.197 pmid:11701822 fatcat:cy36pqijy5hafj7vsqfuy6o5tu

Stabilization of microsatellite sequences by variant repeats in the yeast Saccharomyces cerevisiae

T D Petes, P W Greenwell, M Dominska
1997 Genetics  
We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT).  ...  The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch repair genes pms1, msh2 and msh3, indicating that this effect does not require a functional DNA  ...  In this article we reinvestigate the effects of variant repeats on the stability of the poly GT microsatellite.  ... 
pmid:9178000 pmcid:PMC1207991 fatcat:dtyjkgginve7bkqw3go7kp62nm

Genetic Divergence of Bradyrhizobium Strains Nodulating Soybeans as Revealed by Multilocus Sequence Analysis of Genes Inside and Outside the Symbiosis Island

Xing Xing Zhang, Hui Juan Guo, Rui Wang, Xin Hua Sui, Yan Ming Zhang, En Tao Wang, Chang Fu Tian, Wen Xin Chen, S.-J. Liu
2014 Applied and Environmental Microbiology  
The gene flow level of island genes among genospecies is discussed in the context of the divergence level of off-island genes.  ...  However, seven genes inside the symbiosis island ("island" genes;nifA,nifH,nodC,nodV,fixA,trpD, andrhcC2) showed contrasting lower levels of nucleotide diversity and recombination rates than did off-island  ...  This study was funded by the National Natural Science Foundation of China (31200002).  ... 
doi:10.1128/aem.00044-14 pmid:24632260 pmcid:PMC4018923 fatcat:zsyrtvw4kbfgtoesuwdu6r35ue

TheIn VitroRedundant Enzymes PurN and PurT Are Both Essential for Systemic Infection of Mice in Salmonella enterica Serovar Typhimurium

Lotte Jelsbak, Mie I. B. Mortensen, Mogens Kilstrup, John E. Olsen, A. J. Bäumler
2016 Infection and Immunity  
The results of the present study call for reinvestigations of the concept of metabolic redundancy inS. Typhimuriumin vivo.  ...  In the process of further characterization, we disclosed that the glycine cleavage system (GCV) was the most important for formation of C1unitsin vivo(CI = 0.03 ± 0.03).  ...  ACKNOWLEDGMENTS The technical assistance of Tony Bønnelycke and Pia Mortensen is highly appreciated.  ... 
doi:10.1128/iai.00182-16 pmid:27113361 pmcid:PMC4936368 fatcat:g77dvrvm7baqravpbnbmmzaqva

SEASONAL VARIATIONS IN PHYTOPLANKTON COMMUNITIES OF AN EPHEMERAL POND SYSTEM IN SOUTHEASTERN VIRGINIA

M. Kokolis
2000 Journal of Phycology  
We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis.  ...  Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 .  ...  These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica.  ... 
doi:10.1046/j.1529-8817.1999.00001-111.x fatcat:yjd6u43rv5eofassgcjpsbect4

Reverse Genetics for Type I Feline Coronavirus Field Isolate To Study the Molecular Pathogenesis of Feline Infectious Peritonitis

Rosina Ehmann, Claudia Kristen-Burmann, Barbara Bank-Wolf, Matthias König, Christiane Herden, Torsten Hain, Heinz-Jürgen Thiel, John Ziebuhr, Gergely Tekes, Michael J. Buchmeier
2018 mBio  
Here, we report the first reverse genetics system based on a serotype I FECV field isolate that is suitable to produce high-titer stocks of recombinant FECVs.  ...  To our knowledge, we report the first serotype I FECV field isolate-based reverse genetics system that allows the production of high-titer recombinant virus stocks that can be used for subsequent in vivo  ...  RESULTS Complete genome sequence of a serotype I FECV field isolate. In this study, we aimed to establish a reverse genetics system based on a serotype I FECV field isolate.  ... 
doi:10.1128/mbio.01422-18 pmid:30065095 pmcid:PMC6069117 fatcat:3cimpidqurfclhkgul7qpjbpnm

LIGHT HARVESTING ANTENNAE SYSTEMS: LESSONS FROM SIMPLE ALGAE, THE ROLE OF SCIENTISTS, AND TRENDS

E. Gantt
2000 Journal of Phycology  
We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis.  ...  Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 .  ...  These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica.  ... 
doi:10.1046/j.1529-8817.1999.00001-69.x fatcat:wg3bt4nsg5e7bp2coluezdzruy

SURVEY OF BENTHIC DIATOM COMMUNITIES FROM LOTIC SYSTEMS WITHIN THE WESTERN ALLEGHENY PLATEAU

R. G. Verb, M. L. Vis
2000 Journal of Phycology  
We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis.  ...  Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 .  ...  These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica.  ... 
doi:10.1046/j.1529-8817.1999.00001-201.x fatcat:rjj7dbtzkvc7xafe2j24e52yry

MONITORING THE PERSISTENCE OF GIANT KELP AROUND SANTA CATALINA ISLAND USING A GEOGRAPHIC INFORMATION SYSTEM

W. Bushing
2000 Journal of Phycology  
We hypothesize that a benefit of phagotrophy in G. galatheanum is the acquisition of precursor linolenic acid (18:3n-3) that fuels LC-PUFA synthesis.  ...  Phototrophs grew at 0.37 d Ϫ 1 , while mixotrophs grew at 0.40 d Ϫ 1 with a feeding rate of 0.62 d Ϫ 1 .  ...  These taxa have been differentiated and classified at the generic and species level based primarily on the morphology of their lorica.  ... 
doi:10.1046/j.1529-8817.1999.00001-27.x fatcat:r47z3hmw4bcjlpy5edpg2rxo64

Species-Specific Pharmacological Properties of Human 2A-Adrenoceptors

G. J. Molderings, H. Bonisch, M. Bruss, J. Likungu, M. Gothert
2000 Hypertension  
The antagonistic property of rilmenidine at human ␣ 2A -adrenoceptors indicates that in contrast to the suggestion based on rabbit data, the hypotensive property of the drug in humans is not due to activation  ...  In addition, a partial nucleotide and amino acid sequence of the rabbit ␣ 2A -adrenoceptor (a region known to substantially influence the pharmacological characteristics of the ␣ 2 -adrenoceptor) revealed  ...  Acknowledgments This study was supported by a grant from the Deutsche Forschungsgemeinschaft.  ... 
doi:10.1161/01.hyp.36.3.405 pmid:10988273 fatcat:32dgj7pip5crrprscsoo6dlyly
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