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Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging

Terence T. W. Wong, Andy K. S. Lau, Matthew Y. H. Tang, Kenneth K. Y. Ho, Kenneth K. Y. Wong, Anderson H. C. Shum, Kevin K. Tsia, Daniel L. Farkas, Dan V. Nicolau, Robert C. Leif
2014 Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII  
We have demonstrated a new imaging modality, asymmetric-detection time-stretch optical microscopy (ATOM), by incorporating a simple detection scheme which is a further advancement in time-stretch microscopya  ...  viable solution to achieve high-speed and high-throughput cellular imaging.  ...  Mak for preparing the MIHA, BEL-7402 and HeLa cell lines. We also thank Joseph D. F. Robles for preparing the whole blood sample and the acute leukemic monocytic cells (THP-1).  ... 
doi:10.1117/12.2038952 fatcat:5u44gwubojd5dkpojxgzjyluni

High-throughput single-microparticle imaging flow analyzer

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill (+2 others)
2012 Proceedings of the National Academy of Sciences of the United States of America  
We present an automated flowthrough single-particle optical microscope that overcomes this limitation by performing sensitive blur-free image acquisition and nonstop real-time image-recording and classification  ...  Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications.  ...  Fukuhara for valuable discussions, Y. Liu for assistance with the experiments, and L. Vandenberghe for his suggestion to use the SVM. We acknowledge support from the U.S.  ... 
doi:10.1073/pnas.1204718109 pmid:22753513 pmcid:PMC3406874 fatcat:h2qegow6gjhjdpqju5lyrpn4bi

Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow [article]

Terence T. W. Wong , Matthew Y. H. Tang, Antony C. S. Chan , Godfrey C. F. Chan The University of Hong Kong, Hong Kong These authors contributed equally to this work)
2013 arXiv   pre-print
Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity- a common predicament for advancing high-speed and high-throughput  ...  We here demonstrate a new imaging approach, called asymmetric-detection time-stretch optical microscopy (ATOM), which can deliver ultrafast label-free high-contrast flow imaging with well delineated cellular  ...  Mak for preparing the MIHA, BEL-7402 and HeLa cell lines for us. This  ... 
arXiv:1309.5579v1 fatcat:plwhhgjhmfdg5iczo3p2e33d44

Multi‐ATOM: Ultrahigh‐throughput single‐cell quantitative phase imaging with subcellular resolution

Kelvin C.M. Lee, Andy K.S. Lau, Anson H.L. Tang, Maolin Wang, Aaron T.Y. Mok, Bob M.F. Chung, Wenwei Yan, Ho Cheung Shum, Kathryn S.E. Cheah, Godfrey C.F. Chan, Hayden K.H. So, Kenneth K.Y. Wong (+1 others)
2019 Journal of Biophotonics  
Here we present a new QPI modality, coined multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) that captures and processes quantitative label-free single-cell images at ultrahigh  ...  We employ multi-ATOM for large-scale, label-free, multivariate, cell-type classification (e.g. breast cancer subtypes, and leukemic cells vs peripheral blood mononuclear cells) at high accuracy (>94%).  ...  Technology Support Programme (ITS/090/14, GHP/024/16 GD), and the University Development Funds of the University of Hong Kong.  ... 
doi:10.1002/jbio.201800479 pmid:30719868 pmcid:PMC7065649 fatcat:f56ma3icwzb5vdtxnin6t6y56a

Low-Latency In Situ Image Analytics With FPGA-Based Quantized Convolutional Neural Network

Maolin Wang, Kelvin C. M. Lee, Bob M. F. Chung, Sharatchandra Varma Bogaraju, Ho-Cheung Ng, Justin S. J. Wong, Ho Cheung Shum, Kevin K. Tsia, Hayden Kwok-Hay So
2021 IEEE Transactions on Neural Networks and Learning Systems  
Index Terms-Cell image classification, convolutional neural network (CNN), field-programmable gate array (FPGA), hardware architecture, low-latency inference, multiplexed asymmetric-detection time-stretch  ...  While conventional software-based implementations on the graphic processing unit (GPU)-accelerated platforms are flexible and have achieved very high inference throughput, they are not suitable for latency-sensitive  ...  time-stretch optical microscopy (multi-ATOM), that achieved a high imaging linescan rate of 11.8 MHz [9] .  ... 
doi:10.1109/tnnls.2020.3046452 fatcat:uc5uzptnjjek3cbdg4b64rmvsy

Multi-ATOM: Ultrahigh-throughput single-cell quantitative phase imaging with subcellular resolution: Supplementary files [article]

Kelvin Lee, Andy Lau, Anson Tang, Maolin Wnag, Aaron Mok, Bob Chung, Wenwei Yan, Ho Cheung Shum, Kathryn Cheah, Godfrey Chan, Hayden So, Kenneth Wong (+1 others)
2019 bioRxiv   pre-print
We employ multi-ATOM for large-scale, label-free, multi-variate, cell-type classification (e.g. breast cancer sub-types, and leukemic cells versus peripheral blood mononuclear cells) at high accuracy (  ...  Here we present a new QPI modality, coined multi-ATOM that captures and processes quantitative label-free single-cell images at ultra-high throughput without compromising sub-cellular resolution.  ...  Here we present a new single-cell QPI platform, coined multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) that addresses these challenges and thus fills the gap for achieving  ... 
doi:10.1101/510693 fatcat:qd4ew6lvzjdqnp7eqxiydjvezi

Gate-level Validation of Integrated Circuits with Structured-Illumination Read-out of Embedded Optical Signatures

Negin Zaraee, Boyou Zhou, Kyle Vigil, Mohammad M. Shahjamali, Ajay Joshi, M. Selim Unlu
2020 IEEE Access  
Here, we present a robust, rapid, and nondestructive IC authentication technique, which relies on imaging the optical watermarks embedded in predetermined locations in the IC.  ...  By combining these innovations in plasmonic nanoantennas and optical imaging, we demonstrate through numerical simulations a 30-fold contrast in polarization dependent reflectivity for each embedded optical  ...  ACKNOWLEDGMENT The authors would like to thank Ronen Adato, Aydan Uyar and Bennett Goldberg for their contribution in the early development of this study.  ... 
doi:10.1109/access.2020.2987088 fatcat:uou7rkvdhrdw3cbng674req6ti

Morphological profiling by high-throughput single-cell biophysical fractometry [article]

Ziqi Zhang, Kelvin C.M. Lee, Dickson M.D. Siu, Queenie T.K. Lai, Edmund Y.M. Lam, Kevin K.M. Tsia
2022 bioRxiv   pre-print
the cellular heterogeneity, in the context of classification of lung-cancer cell subtypes and tracking of cell-cycle progression.  ...  Taking together with its high-throughput single-cell imaging performance (~10,000 cells/sec), this technique, termed single-cell biophysical fractometry, offers sufficient statistical power for delineating  ...  and Technology Support Programme (ITS/204/18).  ... 
doi:10.1101/2022.05.24.493226 fatcat:swwpqsarobaifh2l3ubm7zygym

2021 Index IEEE Transactions on Instrumentation and Measurement Vol. 70

2021 IEEE Transactions on Instrumentation and Measurement  
Article numbers are based on specified topic areas and corresponding codes associated with the publication.  ...  Departments and other items may also be covered if they have been judged to have archival value. The Author Index contains the primary entry for each item, listed under the first author's name.  ...  Yang, Y., +, TIM 2021 8504707 Design and Calibration Techniques for a Multichannel FPGA-Based Time-to-Digital Converter in an Object Positioning System.  ... 
doi:10.1109/tim.2022.3156705 fatcat:dmqderzenrcopoyipv3v4vh4ry

National Conference on Recent Advances in Communication Engineering and Information Technology

2020 International journal for innovative engineering and management research  
Our first mission is to educate Electronics and Communication engineers for Indian and Global industries.  ...  We provide a broad-based engineering curriculum, with opportunities for specialization and self-directed learning and development.  ...  Result shows that one model is ideal for real-time application because of speed and the other can be used for more accurate object detection.  ... 
doi:10.48047/ijiemr/v08/spe/02 fatcat:woay334irjgebf5bixuc4ng6em

AbstractBook_OPTO2017.pdf

Michał Mikołajczyk
2017 Figshare  
With the simplicity of production it is a reasonable compromise In this article is presented hybrid diffractive structure consisting of phase encoded as a kinoform in the center and binary phase on the  ...  However, this coding method requires very large resolution and is tough to manufacture. We propose coding the area that is vulnerable for shadow effect with binary phase.  ...  Another option to generate sum of sines is to implement an algorithm on FPGA. FPGAs are known for their speed and this is what we are looking for.  ... 
doi:10.6084/m9.figshare.5146993 fatcat:di5yptwolfa2hjypnxqmyemqby

AbstractBook_OPTO2017.pdf

Michał Mikołajczyk
2017 Figshare  
With the simplicity of production it is a reasonable compromise In this article is presented hybrid diffractive structure consisting of phase encoded as a kinoform in the center and binary phase on the  ...  However, this coding method requires very large resolution and is tough to manufacture. We propose coding the area that is vulnerable for shadow effect with binary phase.  ...  Another option to generate sum of sines is to implement an algorithm on FPGA. FPGAs are known for their speed and this is what we are looking for.  ... 
doi:10.6084/m9.figshare.5146993.v1 fatcat:oaoupvmmgzbpboo32bs5775ypq

A design methodology for 2D sparse NDE arrays using an efficient implementation of refracted-ray TFM

Jerzy Dziewierz, Timothy Lardner, Anthony Gachagan
2013 2013 IEEE International Ultrasonics Symposium (IUS)  
Recent advances in the field of tissue engineering include the engineering of skin, cartilage, and bladder, all of which are relatively thin tissues that can rely on diffusion for the delivery of oxygen  ...  venules) and one that more closely resembles capillaries.  ...  The authors acknowledge the ANR and DGA for their support under the project Metactif, grant ANR-11-ASTR-015.  ... 
doi:10.1109/ultsym.2013.0035 fatcat:th5znfh7y5bklfdbmhb4u6iq2i

Conceptual design and multidisciplinary optimization of in-plane morphing wing structures

Daisaku Inoyama, Brian P. Sanders, James J. Joo, Douglas K. Lindner
2006 Smart Structures and Materials 2006: Modeling, Signal Processing, and Control  
size, distributed sensing, durability, and so on.  ...  The new kind of crack sensor is proper for crack detection of long term structural health monitoring of infrastructures.  ...  Yu, Univ. of Maryland/College Park In this paper, the recently developed high-speed, real-time fiber optic sensor demodulation techniques based on low coherence interferometry and phase-shift- Conference  ... 
doi:10.1117/12.658686 fatcat:rqnvt2atkjgnvpog5pivwjejzm

Characterization and Measurement of Microcomponents with the Atomic Force Microscope (AFM) [chapter]

2018 Optical Inspection of Microsystems  
Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their  ...  Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed.  ...  The main advantages include robustness, a high processing speed, the high degree of flexibility regarding adaptations to new tasks, and the noncontact testing.  ... 
doi:10.1201/9781420019162-8 fatcat:lbc7cnnaxzf7pk7q24mgfcvl5q
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