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Fluorescent and bioluminescent probes and cell function

1997 Journal of Endocrinology  
Measurements can be performed in real-time and provide high resolution information about living cells.  ...  New quantitative measurement technologies enable high speed, low light level fluorescence or luminescence imaging of biological activity on single living cells.  ...  Measurements can be performed in real-time and provide high resolution information about living cells.  ... 
doi:10.1677/joe.0.155sp12 fatcat:flemwum3hvdszhc4ybtnir5rka

Authentic Science Inquiry Learning at Scale Enabled by an Interactive Biology Cloud Experimentation Lab

Zahid Hossain, Engin Bumbacher, Paulo Blikstein, Ingmar Riedel-Kruse
2017 Proceedings of the Fourth (2017) ACM Conference on Learning @ Scale - L@S '17  
We recently developed a scalable cloud lab that enables many online users to perform phototaxis experiment with real, living Euglena cells (opposed to just simulations).  ...  experiments (>2,300), performed data analysis, explored models, and even formulated and experimentally tested their own hypotheses.  ...  A detailed analysis of all student activities and data will be published elsewhere.  ... 
doi:10.1145/3051457.3053994 dblp:conf/lats/HossainBBR17 fatcat:ibcrkmdf4rcohctfogritlcfli

Spontaneous oscillation in cell adhesion and stiffness measured using atomic force microscopy

Hanna Sanyour, Josh Childs, Gerald A. Meininger, Zhongkui Hong
2018 Scientific Reports  
Approaches such as singular spectrum analysis (SSA), and fast Fourier transform (FFT) were introduced to analyze a real-time recording of cell stiffness and the unbinding force between the ECM protein-decorated  ...  the dynamic characteristics of live cells.  ...  H.) and South Dakota Board of Regents UP1600205 (to Z. H.).  ... 
doi:10.1038/s41598-018-21253-9 pmid:29440673 pmcid:PMC5811453 fatcat:mbw7cv5ekzgg7lmsjjlqrs4cg4

Real time visualization of cancer cell death, survival and proliferation using fluorochrome-transfected cells in an IncuCyte® imaging system

Thomas M Lanigan, Stephanie M Rasmussen, Daniel P Weber, Kalana S Athukorala, Phillip L Campbell, David A Fox, Jeffrey H Ruth
2020 Journal of Biological Methods  
Real-time cell analysis technologies that examine the dynamic interactions between cancer cells and the cells of the immune system are becoming more important for assessment of novel therapeutics.  ...  The IncuCyte® system tracks living cells, labeled by a red fluorescent protein, and cell death, as indicated by the caspase-3/7 reagent, which generates a green fluorescent signal upon activation of apoptotic  ...  The authors would also like to thank the University of Michigan Vector Core for generously providing technical services that were critical for the completion of this study.  ... 
doi:10.14440/jbm.2020.323 pmid:32577423 pmcid:PMC7300428 fatcat:qbiqcfsr6nduxbrqh27k3sksw4

Surface Plasmon Resonance for Cell-Based Clinical Diagnosis

Yuhki Yanase, Takaaki Hiragun, Kaori Ishii, Tomoko Kawaguchi, Tetsuji Yanase, Mikio Kawai, Kenji Sakamoto, Michihiro Hide
2014 Sensors  
Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences.  ...  Using SPR sensors, we and other groups have developed techniques to evaluate living cells' reactions in response to stimuli without any labeling in a real-time manner.  ...  Acknowledgments We wish to thank the Analysis Center of Life Science, Hiroshima University for the use of their facilities.  ... 
doi:10.3390/s140304948 pmid:24618778 pmcid:PMC4003976 fatcat:aclzeqllx5hxbg2az2cjkcviqe

An accessible and high-throughput strategy of continuously monitoring apoptosis by fluorescent detection of caspase activation

Kelly M Hanson, Jacob N Finkelstein
2018 Analytical Biochemistry  
We present a real-time, high-throughput, and cost-effective method of detecting apoptosis in vitro using a previously developed reagent that detects caspase activation by fluorescence.  ...  in real time.  ...  Anu Konduru for assistance with the IncuCyte® S3 Live-Cell Analysis System, Devin Chandler-Militello for providing HT1080 cells, and Dr. Tanzy Love for statistics advice.  ... 
doi:10.1016/j.ab.2018.10.022 pmid:30365977 pmcid:PMC6397776 fatcat:hifbmxwppradnkn4gzzt2he47m

A Small-Molecule FRET Reporter for the Real-Time Visualization of Cell-Surface Proteolytic Enzyme Functions

Jing Mu, Fang Liu, Muhammad Shafiq Rajab, Meng Shi, Shuang Li, Chiching Goh, Lei Lu, Qing-Hua Xu, Bin Liu, Lai Guan Ng, Bengang Xing
2014 Angewandte Chemie International Edition  
This small-molecule fluorescent probe may serve as a unique and reliable reporter for real-time visualization of endogenous cell-surfaceassociated proteolytic furin-like enzyme functions in live cells  ...  Real-time imaging of cell-surface-associated proteolytic enzymes is critical to better understand their performances in both physiological and pathological processes.  ...  Moreover, this unique reporter also provides a significant opportunity for the real-time visualization of specific extracellular proteolytic enzyme activities in living cells and tissues.  ... 
doi:10.1002/anie.201407182 pmid:25348774 fatcat:erl7pa6q4bbzhot5gsrzb7gsdu

Designing a nano-interface in a microfluidic chip to probe living cells: Challenges and perspectives

B. P. Helmke, A. R. Minerick
2006 Proceedings of the National Academy of Sciences of the United States of America  
Finally, we explore future directions and critical needs for engineering the cell-chip interface to create an integrated system capable of highresolution analysis and control of cellular physiology.  ...  Second, we present emerging experimental and conceptual platforms for probing living cells with nanotechnology-based tools in a microfluidic chip.  ...  complex biological processes in real time.  ... 
doi:10.1073/pnas.0507304103 pmid:16618928 pmcid:PMC1458901 fatcat:rgxurl5ikjhp5py2koiz7m77wi

Monitoring focal adhesion kinase phosphorylation dynamics in live cells

Nur P. Damayanti, Kevin Buno, Nagarajan Narayanan, Sherry L. Voytik Harbin, Meng Deng, Joseph M. K. Irudayaraj
2017 The Analyst  
FAK FLIM-peptide biosensor enables real-time monitoring of FAK phopshorylation activity.  ...  Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions.  ...  The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Heart, Lung, and Blood Institute or the National Institutes of Health.  ... 
doi:10.1039/c7an00471k pmid:28589989 pmcid:PMC5531600 fatcat:purpqh4ohzftbn6iblxekhpeve

Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

Paul Timpson, Alan Serrels, Marta Canel, Margaret C. Frame, Valerie G. Brunton, Kurt I. Anderson
2009 Cell Adhesion & Migration  
In vivo analysis of E-cadherin dynamics showed that changes in the mobility of E-cadherin can also be used as an 'active' marker of cell behavior in live animals, and may be useful in predicting cell mobilisation  ...  , both the dynamics of cell-cell junctions, as visualised using E-cadherin:GFP, and the dynamics of the plasma membrane, which also plays a Real-time in vivo imaging of metastasis Figure 1 .  ... 
doi:10.4161/cam.3.4.9460 pmid:19690469 pmcid:PMC2802745 fatcat:kpcaqoxgh5g4bigoi5qgtzlt2u

The Need for Thorough in Vitro Testing of Biomaterial Scaffolds: Two Case Studies

David S. Musson, Julie McIntosh, Karen E. Callon, Ashika Chhana, P. Rod Dunbar, Dorit Naot, Jillian Cornish
2013 Procedia Engineering  
While, phenotypic retention was assessed through real-time PCR analysis of tenocytic genes.  ...  Primary human dendritic cells were exposed to scaffolds, with FACS analysis of cell-surface activation markers determining scaffold immunogenicity.  ...  These funding sources had no involvement in the study design; in the collection, analysis and interpretation data; in the writing of the report; nor in the decision to submit the paper for publication.  ... 
doi:10.1016/j.proeng.2013.05.103 fatcat:r6i3g3p4rfftbgnwh5wr7msngy

Silicon Nanomaterials for Biosensing and Bioimaging Analysis

Xiaoyuan Ji, Houyu Wang, Bin Song, Binbin Chu, Yao He
2018 Frontiers in Chemistry  
We especially illustrate the advanced applications of silicon nanomaterials in the field of ultrasensitive biomolecular detection and dynamic biological imaging analysis, with a focus on real-time and  ...  Biochemical analysis in reliable, low-toxicity, and real-time manners are essentially important for exploring and unraveling biological events and related mechanisms.  ...  In particular, benefiting from the nonor low toxicity and high photostability of SiNPs, SiNPs-based fluorescent probes are superbly suitable to track cell-biological interactions in living cells in a real-time  ... 
doi:10.3389/fchem.2018.00038 pmid:29541633 pmcid:PMC5835616 fatcat:2o7skxyksrbcrlgxa64fwy2gdq

Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors

A. Belien
2006 Molecular Cancer Therapeutics  
Real-time analysis of gene expression in experimental tumor models represents a major tool to document disease biology and evaluate disease treatment.  ...  In response to histone deacetylase inhibitors, induction of fluorescence in A2780 ovarian tumors could be monitored in living mice in a noninvasive real-time manner using whole-body imaging.  ...  Jorge Vialard for critical reading of the manuscript.  ... 
doi:10.1158/1535-7163.mct-06-0112 pmid:16985066 fatcat:loojuszs2bftdm3p3ii7knczwi

A Small-Molecule FRET Reporter for the Real-Time Visualization of Cell-Surface Proteolytic Enzyme Functions

Jing Mu, Fang Liu, Muhammad Shafiq Rajab, Meng Shi, Shuang Li, Chiching Goh, Lei Lu, Qing-Hua Xu, Bin Liu, Lai Guan Ng, Bengang Xing
2014 Angewandte Chemie  
This small-molecule fluorescent probe may serve as a unique and reliable reporter for real-time visualization of endogenous cell-surfaceassociated proteolytic furin-like enzyme functions in live cells  ...  Real-time imaging of cell-surface-associated proteolytic enzymes is critical to better understand their performances in both physiological and pathological processes.  ...  Moreover, this unique reporter also provides a significant opportunity for the real-time visualization of specific extracellular proteolytic enzyme activities in living cells and tissues.  ... 
doi:10.1002/ange.201407182 fatcat:6ubem6kvojf2jddqhlsrvpp7r4

An ultra-sensitive and easy-to-use assay for sensing human UGT1A1 activities in biological systems

Ya-Di Zhu, Hui-Lin Pang, Qi-Hang Zhou, Zi-Fei Qin, Qiang Jin, Moshe Finel, Yi-Nan Wang, Wei-Wei QIN, Yin Lu, Dan-Dan Wang, Guang-Bo Ge
2020 Journal of Pharmaceutical Analysis  
Furthermore, the newly developed assay has also been successfully used to screen and characterize the regulatory effects of small molecules on the expression level of UGT1A1 in living cells.  ...  of lower amounts of active enzyme than any other method.  ...  Acknowledgments This work was finically supported by the NSF of China  ... 
doi:10.1016/j.jpha.2020.05.005 pmid:32612873 pmcid:PMC7322753 fatcat:nhbmv3263zci3divdrb5vc5pfm
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