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Perylenetetracarboxylic anhydride as a precursor of fluorescent carbon nanoonion rings

Herme G. Baldoví, José Raul Herance, Víctor Manuel Víctor, Mercedes Alvaro, Hermenegildo Garcia
2015 Nanoscale  
.; Herance Camacho, JR.; Alvaro Rodríguez, MM.; García Gómez, H. (2015). Perylenetetracarboxylic anhydride as a precursor of fluorescent carbon nanoonion rings. Nanoscale. 7(29):  ...  Baldoví, a José Raul Herance, 1a,b Víctor Manuel Víctor, b,c,d Mercedes Alvaro, a and Hermenegildo Garcia *,a,e Thermal annealing at 400 ºC of perylenetetracarboxylic anhydride in low molecular mass PEG  ... 
doi:10.1039/c5nr02903a pmid:26135910 fatcat:sabbfqd7y5avbpbykjxdo2slua

Metabolic footprint of aging and obesity in red blood cells

Inés Domingo-Ortí, Rubén Lamas-Domingo, Andreea Ciudin, Cristina Hernández, José Raúl Herance, Martina Palomino-Schätzlein, Antonio Pineda-Lucena
2021 Aging  
Aging is a physiological process whose underlying mechanisms are still largely unknown. The study of the biochemical transformations associated with aging is crucial for understanding this process and could translate into an improvement of the quality of life of the aging population. Red blood cells (RBCs) are the most abundant cells in humans and are involved in essential functions that could undergo different alterations with age. The present study analyzed the metabolic alterations
more » ... d by RBCs during aging, as well as the influence of obesity and gender in this process. To this end, the metabolic profile of 83 samples from healthy and obese patients was obtained by Nuclear Magnetic Resonance spectroscopy. Multivariate statistical analysis revealed differences between Age-1 (≤45) and Age-2 (>45) subgroups, as well as between BMI-1 (<30) and BMI-2 (≥30) subgroups, while no differences were associated with gender. A general decrease in the levels of amino acids was detected with age, in addition to metabolic alterations of glycolysis, the pentose phosphate pathway, nucleotide metabolism, glutathione metabolism and the Luebering-Rapoport shunt. Obesity also had an impact on the metabolomics profile of RBCs; sometimes mimicking the alterations induced by aging, while, in other cases, its influence was the opposite, suggesting these changes could counteract the adaptation of the organism to senescence.
doi:10.18632/aging.202693 pmid:33609087 pmcid:PMC7950240 fatcat:lciwi3cut5abljeswkk6qutvce

Metabolic fingerprint of insulin resistance in human polymorphonuclear leucocytes

Martina Palomino-Schätzlein, Rafael Simó, Cristina Hernández, Andreea Ciudin, Pablo Mateos-Gregorio, Antonio Hernández-Mijares, Antonio Pineda-Lucena, José Raúl Herance, Clemens Fürnsinn
2018 PLoS ONE  
(TIF) Author Contributions Conceptualization: Martina Palomino-Schätzlein, Rafael Simó, José Raúl Herance. Data curation: Andreea Ciudin, Antonio Hernández-Mijares, José Raúl Herance.33.  ... 
doi:10.1371/journal.pone.0199351 pmid:30005063 pmcid:PMC6044522 fatcat:lmcntrztynhbrnxlohdpkp3see

Mitochondrial Antioxidants Alleviate Oxidative and Nitrosative Stress in a Cellular Model of Sepsis

Nadezda Apostolova, Remedios Garcia-Bou, Antonio Hernandez-Mijares, Raul Herance, Milagros Rocha, Victor M. Victor
2011 Pharmaceutical Research  
Purpose Mitochondrial dysfunction plays a key role in sepsis. Methods We used a sepsis model of human endothelial cells (HUVEC) to study mitochondrial function during normoxic (21% O 2 ) and hypoxic (1% O 2 ) conditions. Results When stimulated with a LPS cocktail, HUVEC displayed an increase of nitric oxide (NO) in normoxic and hipoxic conditions, being higher at 21% O 2 . LPS-activation for 24 h at 1% O 2 increased ROS production, which was reversed with the mitochondrial antioxidant
more » ... ne (MQ) and Glutathione Ethyl Ester (GEE). Activated cells displayed diminished mitochondrial O 2 consumption with specific inhibition of Complex I, accompanied by increase in tyrosine nitration and Type II NOS protein expression, effects which were recovered by antioxidants and/or with L-NAME. These parameters varied with O 2 environment, namely inhibition of respiration observed in both O 2 environments at 24 h was very similar, whereas O 2 consumption rate fell earlier in 1% O 2 -exposed cells. While no significant differences were detected at earlier time points, at 24 h tyrosine nitration was higher in normoxic vs. hypoxic cells. Conclusions Mitochondria are heavily implicated in sepsis. Mitochondrial antioxidants provide a mechanistic model for the development of potential therapies.
doi:10.1007/s11095-011-0528-0 pmid:21786065 fatcat:av35s4txgzda5ayrvcxrb4zjri

Ceria nanoparticles with rhodamine B as a powerful theranostic agent against intracellular oxidative stress

Nadezda Apostolova, Susana Rovira-Llopis, Herme G. Baldoví, Sergio Navalon, Abdullah M. Asiri, Victor M. Victor, Hermenegildo Garcia, Jose Raul Herance
2015 RSC Advances  
Ceria nanoparticles with rhodamine B (RhB-CeNPs) are a new class of biocompatible nanomaterial with antioxidant activity and sensor capacity against oxidant species both in solution and in human cells.
doi:10.1039/c5ra12794g fatcat:n6vkpse7arh2vmu5z7uasoonxq

A translational approach to assess the metabolomic impact of stabilized gold nanoparticles by NMR spectroscopy

José Raul Herance, Hermenegildo García, Patricia Gutiérrez-Carcedo, Sergio Navalón, Antonio Pineda-Lucena, Martina Palomino-Schätzlein
2019 The Analyst  
The metabolomic impact of gold nanoparticles on a cell model can be modulated by a stabilizer.
doi:10.1039/c8an01827h fatcat:ersgl2xhlrhjnmdl3fbeusf5xe

Evidence for a relationship between mitochondrial Complex I activity and mitochondrial aldehyde dehydrogenase during nitroglycerin tolerance: Effects of mitochondrial antioxidants

Remedios Garcia-Bou, Milagros Rocha, Nadezda Apostolova, Raul Herance, Antonio Hernandez-Mijares, Victor M. Victor
2012 Biochimica et Biophysica Acta - Bioenergetics  
The medical use of nitroglycerin (GTN) is limited by patient tolerance. The present study evaluated the role of mitochondrial Complex I in GTN biotransformation and the therapeutic effect of mitochondrial antioxidants. The development of GTN tolerance (in rat and human vessels) produced a decrease in mitochondrial O 2 consumption. Co-incubation with the mitochondria-targeted antioxidant mitoquinone (MQ, 10 − 6 mol/L) or with glutathione ester (GEE, 10 − 4 mol/L) blocked GTN tolerance and the
more » ... ects of GTN on mitochondrial respiration and aldehyde dehydrogenase 2 (ALDH-2) activity. Biotransformation of GTN depended on the mitochondria being functionally active, particularly mitochondrial Complex I. Tolerance induced mitochondrial ROS production and oxidative stress, though these effects were not detected in HUVECρ 0 cells or Complex I mutant cells. Experiments performed to evaluate Complex I-dependent respiration demonstrated that its inhibition by GTN was prevented by the antioxidants in control samples. These results point to a key role for mitochondrial Complex I in the adequate functioning of ALDH-2. In addition, we have identified mitochondrial Complex I as one of the targets at which the initial oxidative stress responsible for GTN tolerance takes place. Our data also suggest a role for mitochondrial-antioxidants as therapeutic tools in the control of the tolerance that accompanies chronic nitrate use.
doi:10.1016/j.bbabio.2012.02.013 pmid:22366168 fatcat:6k6am6pe3vduzmvghbbwtbz3aq

Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1H-NMR spectroscopy, a novel translational approach on a patient-specific basis

Martina Palomino-Schätzlein, Hermenegildo García, Patricia Gutiérrez-Carcedo, Antonio Pineda-Lucena, José Raul Herance, Abhijit De
2017 PLoS ONE  
Methodology: Patricia Gutiérrez-Carcedo, José Raul Herance.  ...  (DOCX) Conceptualization: Martina Palomino-Schätzlein, Hermenegildo García, José Raul Herance. curation: Martina Palomino-Schätzlein, Patricia Gutiérrez-Carcedo, José Raul Herance. analysis: Martina Palomino-Schätzlein  ... 
doi:10.1371/journal.pone.0182985 pmid:28793337 pmcid:PMC5549967 fatcat:ryyjfvrfdjfbtlhoezxwvniq2m

Oncolytic Adenoviruses Armed with Thymidine Kinase Can Be Traced by PET Imaging and Show Potent Antitumoural Effects by Ganciclovir Dosing

Daniel Abate-Daga, Nuria Andreu, Juan Camacho-Sánchez, Ramon Alemany, Raúl Herance, Olga Millán, Cristina Fillat, Maria G. Castro
2011 PLoS ONE  
Replication-competent adenoviruses armed with thymidine kinase (TK) combine the concepts of virotherapy and suicide gene therapy. Moreover TK-activity can be detected by noninvasive positron emission-computed tomography (PET) imaging, what could potentially facilitate virus monitoring in vivo. Here, we report the generation of a novel oncolytic adenovirus that incorporates the Tat8-TK gene under the control of the Major Late Promoter in a highly selective backbone thus providing selectivity by
more » ... argeting the retinoblastoma pathway. The selective oncolytic TK virus, termed ICOVIR5-TK-L, showed reduced potency compared to a non-selective counterpart. However the combination of ICOVIR5-TK-L with ganciclovir (GCV) induced a potent antitumoural effect similar to that of wild type adenovirus in a preclinical model of pancreatic cancer. Although the treatment with GCV provoked a reduction in the viral yield, both in vitro and in vivo, a twocycle treatment of virus and GCV resulted in an enhanced antitumoral response that correlated with high TK-activity, based on microPET measurements. Thus, TK-expressing oncolytic adenoviruses can be traced by PET imaging providing real time information on the activity of the virus and its antitumoral potency can be optimized by GCV dosing.
doi:10.1371/journal.pone.0026142 pmid:22028820 pmcid:PMC3196510 fatcat:iircpv5hrnfvlfqb2mctpeog3u

Alteration of the Mitochondrial Effects of Ceria Nanoparticles by Gold: An Approach for the Mitochondrial Modulation of Cells Based on Nanomedicine

Patricia Gutiérrez-Carcedo, Sergio Navalón, Rafael Simó, Xavier Setoain, Carolina Aparicio-Gómez, Ibane Abasolo, Victor Manuel Victor, Hermenegildo García, José Raúl Herance
2020 Nanomaterials  
Ceria nanoparticles are cell compatible antioxidants whose activity can be enhanced by gold deposition and by surface functionalization with positive triphenylphosphonium units to selectively target the mitochondria. The antioxidant properties of these nanoparticles can serve as the basis of a new strategy for the treatment of several disorders exhibiting oxidative stress, such as cancer, diabetes or Alzheimer's disease. However, all of these pathologies require a specific antioxidant according
more » ... with their mechanism to remove oxidant species excess in cells and diminish their effect on mitochondrial function. The mechanism through which ceria nanoparticles neutralize oxidative stress and their effect on mitochondrial function have not been characterized yet. In the present study, the mitochondria antioxidant effect of ceria and ceria-supported gold nanoparticles, with or without triphenylphosphonium functionalization, was assessed in HeLa cells. The effect caused by ceria nanoparticles on mitochondria function in terms of mitochondrial membrane potential (∆Ψm), adenosine triphosphate (ATP) production, nuclear respiratory factor 1 (NRF1) and nuclear factor erythroid–2–like 1 (NFE2L1) was reversed by the presence of gold. Furthermore, this effect was enhanced when nanoparticles were functionalized with triphenylphosphonium. Our study illustrates how the mitochondrial antioxidant effect induced by ceria nanoparticles can be modulated by the presence of gold.
doi:10.3390/nano10040744 pmid:32295053 pmcid:PMC7221686 fatcat:wi67ws27cfetnienq4ekj5nyyu

Imaging Cortical Dopamine D1 Receptors Using [11C]NNC112 and Ketanserin Blockade of the 5-HT2A Receptors

Ana M Catafau, Graham E Searle, Santiago Bullich, Roger N Gunn, Eugenii A Rabiner, Raul Herance, Joaquim Radua, Magi Farre, Marc Laruelle
2009 Journal of Cerebral Blood Flow and Metabolism  
11 C]NNC112 (8-chloro-7-hydroxy-3-methyl-5-(7-benzofuranyl)-2,3,4,5-tetrahydro-IH-3-benzazepine), a selective positron-emission tomography (PET) ligand for the D 1 receptor (R) over the 5-HT 2A R in vitro, has shown lower selectivity in vivo, hampering measurement of D 1 R in the cortex. [ 11 C]NNC112 PET and intravenous (i.v) ketanserin challenge were used to (1) confirm the previous findings of [ 11 C]NNC112 in vivo D 1 R selectivity, and (2) develop a feasible methodology for imaging
more » ... D 1 R without contamination by 5-HT 2A R. Seven healthy volunteers underwent [ 11 C]NNC112 PET scans at baseline and after a 5-HT 2A R-blocking dose of ketanserin (0.15 mg/kg, i.v.). Percent BP ND change between the post-ketanserin and baseline scans was calculated. Irrespective of the quantification method used, ketanserin pretreatment led to significant decrease of BP ND in the cortical (B30%) and limbic regions (B20%) but not in the striatum, which contains a much lower amount of 5-HT 2A R. Therefore, ketanserin allows D 1 R signal to be detected by [ 11 C]NNC112 PET without significant 5-HT 2A R contamination. These data confirm the presence of a significant 5-HT 2A R contribution to cortical [ 11 C]NNC112 signal, and call for caution in the interpretation of published [ 11 C]NNC112 PET findings on cortical D 1 R in humans. In the absence of more selective ligands, [ 11 C]NNC112 PET with ketanserin can be used for cortical D 1 R imaging in vivo.
doi:10.1038/jcbfm.2009.269 pmid:20029452 pmcid:PMC2949183 fatcat:cokbhmf3obhlhb2tad45epuwau

Correction: Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1H-NMR spectroscopy, a novel translational approach on a patient-specific basis

Martina Palomino-Schätzlein, Hermenegildo García, Patricia Gutiérrez-Carcedo, Antonio Pineda-Lucena, José Raul Herance
2017 PLoS ONE  
Reference 1 . 1 Palomino-Schätzlein M, García H, Gutiérrez-Carcedo P, Pineda-Lucena A, Herance JR (2017) Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1 H-NMR  ...  journal.pone.0189748 December 11, 2017 1 / 1 a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 OPEN ACCESS Citation: Palomino-Schätzlein M, García H, Gutiérrez-Carcedo P, Pineda-Lucena A, Herance  ... 
doi:10.1371/journal.pone.0189748 pmid:29228049 pmcid:PMC5724867 fatcat:5ui7wq5mcnhdvi2xppopglxo7e

Bilastinevs.hydroxyzine: occupation of brain histamine H1-receptors evaluated by positron emission tomography in healthy volunteers

Magí Farré, Clara Pérez-Mañá, Esther Papaseit, Esther Menoyo, Marta Pérez, Soraya Martin, Santiago Bullich, Santiago Rojas, José-Raúl Herance, Carlos Trampal, Luis Labeaga, Román Valiente
2014 British Journal of Clinical Pharmacology  
AIM A close correlation exists between positron emission tomography (PET)-determined histamine H1-receptor occupancy (H1RO) and the incidence of sedation. Antihistamines with H1RO <20% are classified as non-sedating. The objective was to compare the H1RO of bilastine, a second generation antihistamine, with that of hydroxyzine. METHODS This randomized, double-blind, crossover study used PET imaging with [ 11 C]-doxepin to evaluate H1RO in 12 healthy males (mean age 26.2 years), after single
more » ... administration of bilastine (20 mg), hydroxyzine (25 mg) or placebo. Binding potentials and H1ROs were calculated in five cerebral cortex regions of interest: frontal, occipital, parietal, temporal, insula. Plasma bilastine concentrations, subjective sedation (visual analogue scale), objective psychomotor performance (digital symbol substitution test), physiological variables and safety (adverse events, AEs), were also evaluated.
doi:10.1111/bcp.12421 pmid:24833043 pmcid:PMC4243871 fatcat:gvd366zb7re5tcl3gtdzfro2vi

A Translational In Vivo and In Vitro Metabolomic Study Reveals Altered Metabolic Pathways in Red Blood Cells of Type 2 Diabetes

Martina Palomino-Schätzlein, Rubén Lamas-Domingo, Andreea Ciudin, Patricia Gutiérrez-Carcedo, Rosó Marés, Carolina Aparicio-Gómez, Cristina Hernández, Rafael Simó, José Raúl Herance
2020 Journal of Clinical Medicine  
Clinical parameters used in type 2 diabetes mellitus (T2D) diagnosis and monitoring such as glycosylated haemoglobin (HbA1c) are often unable to capture important information related to diabetic control and chronic complications. In order to search for additional biomarkers, we performed a pilot study comparing T2D patients with healthy controls matched by age, gender, and weight. By using 1H-nuclear magnetic resonance (NMR) based metabolomics profiling of red blood cells (RBCs), we found that
more » ... he metabolic signature of RBCs in T2D subjects differed significantly from non-diabetic controls. Affected metabolites included glutathione, 2,3-bisphophoglycerate, inosinic acid, lactate, 6-phosphogluconate, creatine and adenosine triphosphate (ATP) and several amino acids such as leucine, glycine, alanine, lysine, aspartate, phenylalanine and tyrosine. These results were validated by an independent cohort of T2D and control patients. An analysis of the pathways in which these metabolites were involved showed that energetic and redox metabolism in RBCs were altered in T2D, as well as metabolites transported by RBCs. Taken together, our results revealed that the metabolic profile of RBCs can discriminate healthy controls from T2D patients. Further research is needed to determine whether metabolic fingerprint in RBC could be useful to complement the information obtained from HbA1c and glycemic variability as well as its potential role in the diabetes management.
doi:10.3390/jcm9061619 pmid:32471219 pmcid:PMC7355709 fatcat:iwujltqpjncupddv2pup6mbhze

Modelling the skeletal muscle injury recovery using in vivo contrast-enhanced micro-CT: a proof-of-concept study in a rat model

Bruno Paun, Daniel García Leon, Alex Claveria Cabello, Roso Mares Pages, Elena de la Calle Vargas, Paola Contreras Muñoz, Vanessa Venegas Garcia, Joan Castell-Conesa, Mario Marotta Baleriola, Jose Raul Herance Camacho
2020 European Radiology Experimental  
Skeletal muscle injury characterisation during healing supports trauma prognosis. Given the potential interest of computed tomography (CT) in muscle diseases and lack of in vivo CT methodology to image skeletal muscle wound healing, we tracked skeletal muscle injury recovery using in vivo micro-CT in a rat model to obtain a predictive model. Skeletal muscle injury was performed in 23 rats. Twenty animals were sorted into five groups to image lesion recovery at 2, 4, 7, 10, or 14 days after
more » ... y using contrast-enhanced micro-CT. Injury volumes were quantified using a semiautomatic image processing, and these values were used to build a prediction model. The remaining 3 rats were imaged at all monitoring time points as validation. Predictions were compared with Bland-Altman analysis. Optimal contrast agent dose was found to be 20 mL/kg injected at 400 μL/min. Injury volumes showed a decreasing tendency from day 0 (32.3 ± 12.0mm3, mean ± standard deviation) to day 2, 4, 7, 10, and 14 after injury (19.6 ± 12.6, 11.0 ± 6.7, 8.2 ± 7.7, 5.7 ± 3.9, and 4.5 ± 4.8 mm3, respectively). Groups with single monitoring time point did not yield significant differences with the validation group lesions. Further exponential model training with single follow-up data (R2 = 0.968) to predict injury recovery in the validation cohort gave a predictions root mean squared error of 6.8 ± 5.4 mm3. Further prediction analysis yielded a bias of 2.327. Contrast-enhanced CT allowed in vivo tracking of skeletal muscle injury recovery in rat.
doi:10.1186/s41747-020-00163-4 pmid:32488324 fatcat:pby6hy7onvbwjbqdhs7knnvqti
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