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PPARγ agonists identified in extracts of elderflowers (Sambucus nigra) by bioassay-guided fractionation

KB Christensen, K Grevsen, K Petersen Rasmus, K Kristiansen, LP Christensen
2008 Planta Medica  
Christensen 1 , Kai Grevsen 2 , Rasmus K. Petersen 3 , Karsten Kristiansen 4 , and Lars P.  ...  Bioactivity was assessed using a PPARγ transactivation assay and results obtained are shown to the left for the four fractions I, J, K, and L.  ... 
doi:10.1055/s-0028-1084145 fatcat:ihxusykwybdhvcjiht4zuv2g6y

Fast detection of extrasynaptic GABA with a whole-cell sniffer

Rasmus K. Christensen, Anders V. Petersen, Nicole Schmitt, Jean-François Perrier
2014 Frontiers in Cellular Neuroscience  
Petersen, performed experiments. Rasmus K. Christensen analyzed data. Rasmus K. Christensen and Jean-François Perrier interpreted results of experiments. Rasmus K.  ...  Rasmus K. Christensen, Anders V. Petersen, Nicole Schmitt and Jean-François Perrier approved final version of manuscript. FIGURE 1 | 1 Whole-cell sniffer with GABA A receptors.  ... 
doi:10.3389/fncel.2014.00133 pmid:24860433 pmcid:PMC4030185 fatcat:fsr4dfmcdfgofieimivivqte5i

Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant [article]

Sarah K. Gersing, Yong Wang, Martin Grønbæk-Thygesen, Caroline Kampmeyer, Lene Clausen, Claes Andréasson, Amelie Stein, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen
2020 bioRxiv   pre-print
GAPDH and the Na/K ATPase serve as a loading control for the soluble and insoluble protein fraction, respectively.  ...  Blotting for GAPDH and the Na/K ATPase is used as a loading control for the soluble and the insoluble fractions respectively. . CC-BY-NC-ND 4.0 International license perpetuity.  ... 
doi:10.1101/2020.09.04.283028 fatcat:fpapn4wg3rbd5l7urcoe56sszy

Deregulated MAPK Activity Prevents Adipocyte Differentiation of Fibroblasts Lacking the Retinoblastoma Protein

Jacob B. Hansen, Rasmus K. Petersen, Claus Jørgensen, Karsten Kristiansen
2002 Journal of Biological Chemistry  
In agreement with previous reports, this assay results in two bands, which represent either different posttranslationally modified forms of Ras or K-Ras and N-Ras (upper and lower bands, respectively)  ... 
doi:10.1074/jbc.m203870200 pmid:12000769 fatcat:byiyzqorprg4jgsb3minfsx5x4

Co-Chaperones in Targeting and Delivery of Misfolded Proteins to the 26S Proteasome

Amanda B. Abildgaard, Sarah K. Gersing, Sven Larsen-Ledet, Sofie V. Nielsen, Amelie Stein, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen
2020 Biomolecules  
Protein homeostasis (proteostasis) is essential for the cell and is maintained by a highly conserved protein quality control (PQC) system, which triages newly synthesized, mislocalized and misfolded proteins. The ubiquitin-proteasome system (UPS), molecular chaperones, and co-chaperones are vital PQC elements that work together to facilitate degradation of misfolded and toxic protein species through the 26S proteasome. However, the underlying mechanisms are complex and remain partly unclear.
more » ... e, we provide an overview of the current knowledge on the co-chaperones that directly take part in targeting and delivery of PQC substrates for degradation. While J-domain proteins (JDPs) target substrates for the heat shock protein 70 (HSP70) chaperones, nucleotide-exchange factors (NEFs) deliver HSP70-bound substrates to the proteasome. So far, three NEFs have been established in proteasomal delivery: HSP110 and the ubiquitin-like (UBL) domain proteins BAG-1 and BAG-6, the latter acting as a chaperone itself and carrying its substrates directly to the proteasome. A better understanding of the individual delivery pathways will improve our ability to regulate the triage, and thus regulate the fate of aberrant proteins involved in cell stress and disease, examples of which are given throughout the review.
doi:10.3390/biom10081141 pmid:32759676 pmcid:PMC7463752 fatcat:2jjy4wrjcfbjvkam7ixqtasbd4

Mapping the degradation pathway of a disease-linked aspartoacylase variant

Sarah K. Gersing, Yong Wang, Martin Grønbæk-Thygesen, Caroline Kampmeyer, Lene Clausen, Martin Willemoës, Claes Andréasson, Amelie Stein, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen, Hua Tang
2021 PLoS Genetics  
In addition, the reported very modest enzyme activity (k cat~0 .08 s -1 for N-Acetyl-L-aspartate and k cat~0 .62 s -1 for N-Chloroacetyl-L-aspartate) for wild-type ASPA means that concentrated ASPA preparations  ...  GAPDH and the Na/K ATPase serve as a loading control for the soluble and insoluble protein fraction, respectively.  ... 
doi:10.1371/journal.pgen.1009539 pmid:33914734 fatcat:5qr6bvyy3fhmrgycs36jxflpui

Even effect of milk protein and carbohydrate intake but no further effect of heavy resistance exercise on myofibrillar protein synthesis in older men

Søren Reitelseder, Kasper Dideriksen, Jakob Agergaard, Nikolaj M. Malmgaard-Clausen, Rasmus L. Bechshoeft, Rasmus K. Petersen, Anja Serena, Ulla R. Mikkelsen, Lars Holm
2018 European Journal of Nutrition  
Dideriksen K, Reitelseder S, Malmgaard-Clausen NM, Bechshoeft R, Petersen RK, Mikkelsen UR, Holm L (2016) No effect of antiinflammatory medication on postprandial and postexercise muscle protein synthesis  ...  Dideriksen KJ, Reitelseder S, Petersen SG, Hjort M, Helmark IC, Kjaer M, Holm L (2011) Stimulation of muscle protein synthesis by whey and caseinate ingestion after resistance exercise in elderly individuals  ... 
doi:10.1007/s00394-018-1641-1 pmid:29470691 fatcat:3rjscjyfnran7a6m5hrcng3aie

Insulin-like Growth Factor-1/Insulin Bypasses Pref-1/FA1-mediated Inhibition of Adipocyte Differentiation

Hongbin Zhang, Jane Nøhr, Charlotte H. Jensen, Rasmus K. Petersen, Elin Bachmann, Børge Teisner, Leif K. Larsen, Susanne Mandrup, Karsten Kristiansen
2003 Journal of Biological Chemistry  
In our experiments significant differentiation of Pref-1/FA1-expressing cells was restored using 10 nM IGF-1, which fits well with a K d of 5 nM for binding of IGF-1 to the IGF-1R.  ... 
doi:10.1074/jbc.m300022200 pmid:12651852 fatcat:gms4rnt6arh3ddgfdtzvzblnoi

Mutations in a single signaling pathway allow growth on a different solvent than water [article]

Caroline Kampmeyer, Jens V. Johansen, Christian Holmberg, Magnus Karlson, Sarah K. Gersing, Heloisa N. Bordallo, Birthe B. Kragelund, Mathilde H. Lerche, Isabelle Jourdain, Jakob R. Winther, Rasmus Hartmann-Petersen
2019 bioRxiv   pre-print
Poulsen EG, Kampmeyer C, Kriegenburg F, Johansen JV, Hofmann K, Holmberg C, et al.  ...  The freeze-dried extracts were re-dissolved in 494 a hyperpolarization matrix and polarized at 3.35 T and 1.4 °K.  ... 
doi:10.1101/692889 fatcat:cg7dkrqdmzcyngggwcd3vktori

A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues

Nicklas H Staunstrup, Nynne Sharma, Rasmus O Bak, Lars Svensson, Thomas K Petersen, Lene Aarenstrup, Karsten Kristiansen, Lars Bolund, Jacob Mikkelsen
2011 BMC Biotechnology  
Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection.
more » ... is system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker. Results: The three expression cassettes, inserted in a head-to-tail orientation in a Sleeping Beauty DNA transposon vector, are efficiently inserted as a single genetic entity into the genome of cells of interest in a reaction catalyzed by the hyperactive SB100X transposase. The applicability of the sensor for screening purposes is demonstrated by the functional comparison of potent synthetic analogues of vitamin D3 designed for the treatment of psoriasis and cancer. In clones of human keratinocytes carrying from a single to numerous insertions of the vitamin D3 sensor, a sensitive sensor read-out is detected upon exposure to even low concentrations of vitamin D3 analogues. In comparative studies, the sensor unveils superior potency of new candidate drugs in comparison with analogues that are currently in clinical use. Conclusions: Our findings demonstrate the use of the genetic sensor as a tool in first-line evaluation of new vitamin D3 analogues and pave the way for new types of drug delivery studies in sensor-transgenic animals.
doi:10.1186/1472-6750-11-33 pmid:21473770 pmcid:PMC3083354 fatcat:vkz7l36dezgyldgtsgv6kxc5zq

Activation of Peroxisome Proliferator-activated Receptor γ Bypasses the Function of the Retinoblastoma Protein in Adipocyte Differentiation

Jacob B. Hansen, Rasmus K. Petersen, Berit M. Larsen, Jirina Bartkova, Jan Alsner, Karsten Kristiansen
1999 Journal of Biological Chemistry  
Alsner, and K. Kristiansen, unpublished results.  ...  The ligand concentration needed to bypass the defective differentiation in RB Ϫ/Ϫ MEFs was in agreement with the K d of BRL49653 binding to PPAR␥ (17) in that differentiation was promoted with 50 nM  ... 
doi:10.1074/jbc.274.4.2386 pmid:9891007 fatcat:fovqki4wqrf73frwh5k5beqiwm

Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases

Rasmus K. Petersen, Claus Jørgensen, Arild C. Rustan, Livar Frøyland, Karin Muller-Decker, Gerhard Furstenberger, Rolf K. Berge, Karsten Kristiansen, Lise Madsen
2003 Journal of Lipid Research  
Arachidonic acid inhibits adipocyte differentiation of 3T3-L1 cells via a prostaglandin synthesis-dependent pathway. Here we show that this inhibition requires the presence of a cAMP-elevating agent during the first two days of treatment. Suppression of protein kinase A activity by H-89 restored differentiation in the presence of arachidonic acid. Arachidonic acid treatment led to a prolonged activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2), and suppression of ERK1/2
more » ... y by the addition of U0126 rescued differentiation. Upon induction of differentiation, expression of cyclooxygenase-2 (COX-2) was transiently induced and then declined, whereas COX-1 expression declined gradually as differentiation progressed. Treatment with arachidonic acid led to sustained expression of COX-1 and COX-2. Omission of a cAMP-elevating agent or addition of H-89 or U0126 prevented sustained expression of COX-2. Unexpectedly, we observed that selective COX-1 or COX-2 inhibitors rescued adipocyte differentiation in the presence of arachidonic acid as effectively as did the nonselective COX-inhibitor indomethacin. De novo fatty acid synthesis, diacylglycerol acyltransferase (DGAT) activity, and triacylglycerol accumulation were repressed in cells treated with arachidonic acid. Indomethacin restored DGAT activity and triacylglycerol accumulation without restoring de novo fatty acid synthesis, resulting in an enhanced incorporation of arachidonic acid into cellular triacylglycerolsArachidonic aciddependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases. J. Lipid Res. 2003. 44: 2320-2330. Supplementary key words mitogen-activated protein kinase • peroxisome proliferator-activated receptor • CCAAT/enhancer-binding protein • diacylglycerol acyltransferase • protein kinase A Abbreviations: Akt/PKB, protein kinase B; aP2, adipocyte lipid binding protein; COX, cyclooxygenase; DGAT, diacylglycerol acyltransferase; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; ERK1/2, extracellular signal-regulated kinases 1 and 2; MAPK, mitogen-activated protein kinase; MDI, methylisobutylxanthine, dexamethasone, and insulin; MIX, methylisobutylxanthine; PPAR, peroxisome proliferator-activated receptor; SD, standard deviation; TFIIB, transcription factor IIB.
doi:10.1194/jlr.m300192-jlr200 pmid:12923227 fatcat:2hmwgyxwknh77jgicl3rqhpb24

In-amplifier and cascaded mid-infrared supercontinuum sources with low noise through gain-induced soliton spectral alignment

Kyei Kwarkye, Mikkel Jensen, Rasmus D. Engelsholm, Manoj K. Dasa, Deepak Jain, Patrick Bowen, Peter M. Moselund, Christian R. Petersen, Ole Bang
2020 Scientific Reports  
Petersen and Ole Bang. ✉ e-mail: kkwar@fotonik. dtu.dk open 2 Scientific RepoRtS | (2020) 10:8230 | https://doi.org/10.1038/s41598-020-65150-6 Figure 1 . 1 (a) Experimentally measured PSD (solid lines  ... 
doi:10.1038/s41598-020-65150-6 pmid:32427972 fatcat:iknjtcjq6veh3d3hbm5mamgsum

Activation of Liver X Receptors Prevents Statin-induced Death of 3T3-L1 Preadipocytes

Lise Madsen, Rasmus K. Petersen, Knut R. Steffensen, Lone M. Pedersen, Philip Hallenborg, Tao Ma, Livar Frøyland, Stein Ove Døskeland, Jan-Åke Gustafsson, Karsten Kristiansen
2008 Journal of Biological Chemistry  
However, LXR-mediated rescue of statin-induced cell death proceeded unabated in the presence of inhibitors of PI3-K/PKB and ERK activation, suggesting that LXR-dependent rescue did not require signaling  ... 
doi:10.1074/jbc.m800720200 pmid:18487205 fatcat:jofw4lliobe3npbdfl4xldaplq

Synthesis and biological evaluation of dihydropyrano-[2,3-c]pyrazoles as a new class of PPARγ partial agonists

Katrine Qvortrup, Jakob F. Jensen, Mikael S. Sørensen, Irene Kouskoumvekaki, Rasmus K. Petersen, Olivier Taboureau, Karsten Kristiansen, Thomas E. Nielsen, Andrea Cavalli
2017 PLoS ONE  
Compound 14 was quantitatively converted to aldehyde 15 by reaction with 4-chloro-2-methylphenol in acetonitrile at 50˚C in the presence of K 2 CO 3 .  ...  -2,4-dimethylbenz-aldehyde (15) [17]. 5-Bromomethyl-2,4-dimethylbenzaldehyde (1.0 g, 4.40 mmol) and 4-chloro-2-methylphenol (657 mg, 4.62 mmol) were dissolved in acetonitrile (50 mL) and treated with K  ... 
doi:10.1371/journal.pone.0162642 pmid:28245241 pmcid:PMC5330453 fatcat:bbc6zcxxrjglvgnirf5xcsyv7y
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