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The ADEPT Concept-Based Digital Learning Environment [chapter]

T. R. Smith, D. Ancona, O. Buchel, M. Freeston, W. Heller, R. Nottrott, T. Tierney, A. Ushakov
2003 Lecture Notes in Computer Science  
We describe the design and application of a Digital Learning Environment (DLE) that is integrated with the collections and services of the Alexandria Digital Library (ADL). This DLE is in operational use in undergraduate teaching environments. Its design and development incorporate the assumption that deep understanding of both scientific phenomena and scientific methods is facilitated when learning materials are explicitly organized, accessed and presented at the level of granularity of
more » ... iate sets of scientific concepts and their interrelationships. The DLE supports services for the creating, searching, and displaying: (1) knowledge bases (KBs) of strongly structured models of scientific concepts; (2) DL collections of information objects organized and accessible by the concepts of the KBs; and (3) collections of presentation materials, such as lectures and laboratory materials, that are organized as trajectories through the KB of concepts.
doi:10.1007/978-3-540-45175-4_28 fatcat:mxx4fbdlafabfplmqtejulamre

Glucosylation of Rho GTPases by Clostridium difficile toxin A triggers apoptosis in intestinal epithelial cells

R. Gerhard, S. Nottrott, J. Schoentaube, H. Tatge, A. Olling, I. Just
2008 Journal of Medical Microbiology  
The intestinal epithelial cell line HT-29 was used to study the apoptotic effect of Clostridium difficile toxin A (TcdA). TcdA is a 300 kDa single-chain protein, which glucosylates and thereby inactivates small GTPases of the Rho family (Rho, Rac and Cdc42). The effect of TcdA-catalysed glucosylation of the Rho GTPases is well known: reorganization of the actin cytoskeleton with accompanying morphological changes in cells, leading to complete rounding of cells and destruction of the intestinal
more » ... arrier function. Less is known about the mechanism by which apoptosis is induced in TcdA-treated cells. In this study, TcdA induced the activation of caspase-3, -8 and -9. Apoptosis, as estimated by the DNA content of cells, started as early as 24 h after the addition of TcdA. The impact of Rho glucosylation was obvious when mutant TcdA with reduced or deficient glucosyltransferase activity was applied. TcdA mutant W101A, with 50-fold reduced glucosyltransferase activity, induced apoptosis only at an equipotent concentration compared with wild-type TcdA at a 50 % effective concentration of 0.2 nM. The enzyme-deficient mutant TcdA D285/287N was not able to induce apoptosis. Apoptosis induced by TcdA strictly depended on the activation of caspases, and was completely blocked by the pan-caspase inhibitor z-VAD-fmk. Destruction of the actin cytoskeleton by latrunculin B was not sufficient to induce apoptosis, indicating that apoptosis induced by TcdA must be due to another mechanism. In summary, TcdA-induced apoptosis (cytotoxic effect) depends on the glucosylation of Rho GTPases, but is not triggered by destruction of the actin cytoskeleton (cytopathic effect). Abbreviation: FACS, fluorescence-activated cell sorting.
doi:10.1099/jmm.0.47769-0 pmid:18480335 fatcat:4gfes2pu3zbdpjcvnna34dhlqy

Binding of the Human Prp31 Nop Domain to a Composite RNA-Protein Platform in U4 snRNP

S. Liu, P. Li, O. Dybkov, S. Nottrott, K. Hartmuth, R. Luhrmann, T. Carlomagno, M. C. Wahl
2007 Science  
Kim for analytical assistance, and R. Pelker for assistance with blood serum assays of IGF1.  ...  N indicates any nucleotide; R, purine. Binding of 15.5K and the secondary binding proteins is indicated.  ... 
doi:10.1126/science.1137924 pmid:17412961 fatcat:ecpk6ylusvfl5opscrnjuyz35e

Protein-Protein and Protein-RNA Contacts both Contribute to the 15.5K-Mediated Assembly of the U4/U6 snRNP and the Box C/D snoRNPs

A. Schultz, S. Nottrott, N. J. Watkins, R. Luhrmann
2006 Molecular and Cellular Biology  
The binding of hPRP31 to the U4 snRNA was assayed by the method of Nottrott et al.  ...  CYPH/hPRP4/hPRP3 binding to U4/U6 snRNA was performed by the method of Nottrott et al.  ... 
doi:10.1128/mcb.02374-05 pmid:16782898 pmcid:PMC1489164 fatcat:r4tpt3u3srfcte2no5d7kw2xce

Page 96 of National Union Catalog Vol. 110, Issue [page]

1942 National Union Catalog  
Berlin, R. Giirgens, 1925. 216 p. 22" Literaturverzeichnis” : p. (17)-18 1 Banking law— Germany. 2. Finance omen Money— Germany Law 1.  ...  (Pell name: Carl Gustav Rudolph Edward Alfred Nottrott, 12-19104 Revised Library of Congress PLAGAO.NOT —-——— Copy 2 (Thab2, Nottrott, Carl Gustav Rudolsh Eduard \ifred Nottrott, ilfred, 1837-1924.  ... 

A notation for describing multiple views of VLSI circuits

J.-L. Baer, M.-C. Liem, L. McMurchie, R. Nottrott, L. Snyder, W. Winder
25th ACM/IEEE, Design Automation Conference.Proceedings 1988.  
,l, i n r c i d , route-r, evalCc1ockJ. pnCsolect, clockl, one. zoro; x b ] , clock; yC2**d ; ixrow I (I (dec~rowCrow1) (roo = 2 * * r i . .O)); route-1 --(--(hT[X[COl]]> (col = n-i..O)) -route-r; eral  ...  The second element of the environment is a database or "model" which uides the generation p r e cess.  ... 
doi:10.1109/dac.1988.14743 fatcat:6frhvl2hzrbk3kim2nu46zriri

A Common Core RNP Structure Shared between the Small Nucleoar Box C/D RNPs and the Spliceosomal U4 snRNP

Nicholas J Watkins, Véronique Ségault, Bruno Charpentier, Stephanie Nottrott, Patrizia Fabrizio, Angela Bachi, Matthias Wilm, Michael Rosbash, Christiane Branlant, Reinhard Lührmann
2000 Cell  
The nucleolus contains a The human ortholog of Snu13p, 15.5kD, directly binds multitude of small nucleolar RNAs (snoRNAs), several the 5Ј stem-loop of the U4 snRNA (Nottrott et al., 1999). of which are  ...  The box loop-stem structure (Nottrott et al., 1999) .  ...  into pRS316 to create pRS316SNU13. and grants from the Deutsche Forschungsgemeinschaft (SFB397) Three copies of the HA epitope tag were added to the C terminus and the Fonds der Chemischen Industrie (to R.  ... 
doi:10.1016/s0092-8674(00)00137-9 pmid:11081632 fatcat:67wvis6nqjdjragxtf7p7tikry

Crystal Structure of the Spliceosomal 15.5kD Protein Bound to a U4 snRNA Fragment

Ivan Vidovic, Stephanie Nottrott, Klaus Hartmuth, Reinhard Lührmann, Ralf Ficner
2000 Molecular Cell  
Bringmann, P., Appel, B., Rinke, J., Reuter, R., Theissen, H., and Lü hrmann, R. (1984).  ...  It additionally This protein was shown to play an important part in the late stage of spliceosome assembly before splicing suggests a general recognition principle in a novel catalysis (Nottrott et al.  ...  R-factor ϭ  ... 
doi:10.1016/s1097-2765(00)00131-3 pmid:11163207 fatcat:24sumbexsff55nhkfidbk7yd3e

Long-term mechanical properties of a novel low-modulus bone cement for the treatment of osteoporotic vertebral compression fractures

C. Robo, C. Öhman-Mägi, C. Persson
2021 Journal of The Mechanical Behavior of Biomedical Materials  
Nottrott et al. (2007) reported an increase in CS and E after 1 week followed by a decrease of both properties onwards over a period of 1 year, for Palacos® R cement, which contains 67% less ZrO 2 radio-opacifier  ...  (Nottrott et al., 2007 (Nottrott et al., , 2008 Baleani et al., 2001 ) and were expected.  ... 
doi:10.1016/j.jmbbm.2021.104437 pmid:33706086 fatcat:krvtzgwjoffr3cw4ye436f4tw4

VI. Additions to the Library

1895 Journal of the Royal Asiatic Society of Great Britain & Ireland  
Gundry (R. S.). China, Present and Past. 8vo. London, 1895. By the Kgl. Museum fur Volkerkunde, Berlin. Pander (Eugen).  ...  Nottrott (A.). Grammatik der Kolhsprache. 8vo. Gutersloh, 1882. Skrefsrud (Presented by Mrs. Beveridge. Beveridge (H.). Trial of Nanda Kumar. 8vo. Calcutta, 1886.  ... 
doi:10.1017/s0035869x00145320 fatcat:6czyoxnxtnf5fh6563pdhxkifu

Biochemical defects in minor spliceosome function in the developmental disorder MOPD I

F. Jafarifar, R. C. Dietrich, J. M. Hiznay, R. A. Padgett
2014 RNA: A publication of the RNA Society  
In other work, we show that this mutation causes a splicing defect due to the lengthening or hyperstabilization of stem I (R Dietrich, G Shukla, and R Padgett, in prep.) .  ...  The 15.5K protein belongs to a family of RNA-binding proteins that bind to K-turn motifs through an induced fit interaction (Nottrott et al. 1999 (Nottrott et al. , 2002 Turner et al. 2005; Woźniak et  ... 
doi:10.1261/rna.045187.114 pmid:24865609 pmcid:PMC4114687 fatcat:r5r6eluiyrdibntwtume3ikka4

Page 185 of The American Antiquarian and Oriental Journal Vol. 8, Issue 3 [page]

1886 The American Antiquarian and Oriental Journal  
Nottrott Grammatik der, Kolh-Sprache, pp. 104, Guetersloh, 1882; The Four Gospels in Mundari-Kolh; Rev. L...O.  ...  Clark, Jour R. A. 8. Vol. XI. Part II., and the Gos- pels of Matthew and John and the Life of Joseph in, Ao Naga, by. the same; : The Nagas and Neighboring Tribes, by 8. i. Peele, Jour.  ... 

Page 250 of Christian Literature Vol. 12, Issue 4 [page]

1895 Christian Literature  
NOTTROTT, L. Die Gossnersche Mission un- ter den Kolhs. 2. Jubiliiums-[Titel-]Ausg. Halle (1874. 88), Miihlmann, 1894. x+455, vii+235 p. 8°. 6M. SCHIMMELMANN, A.  ...  R. Oowikapun; or, how the gos- pel reached the Nelson River Indians. N. Y., Hunt & Baton, 1894. 240 p. 12°. $1. b. Other. FUEGUNGEN, unverkennbare, Gottes. Auf-_ zeichnungen e. bekehrten Israeliten.  ... 

Differential association of microRNAs with polysomes reflects distinct strengths of interactions with their mRNA targets

N. Molotski, Y. Soen
2012 RNA: A publication of the RNA Society  
Results in B, C, E, and F are displayed as mean +/À standard error (SE). 0.91 compared, respectively, with R 2 = 0.91 and correlation = 0.95) (Supplemental Fig. S1A,B) .  ...  D, P-value = 0.009 based on Fisher's r-to-z transformation). inverse correlation between abundance and occupancy of microRNAs was much weaker (correlation = À0.23 in hFFs vs.  ... 
doi:10.1261/rna.033142.112 pmid:22836355 pmcid:PMC3425777 fatcat:fjt6xiiacje4heeackrsnbddyu

Page 558 of Cellular and Molecular Life Sciences Vol. 65, Issue 4 [page]

2008 Cellular and Molecular Life Sciences  
Thermann, R. and Hentze, M. W. (2007) Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation. Nature 447, 875-878 Nottrott, S., Simard, M. J. and Richter, J.  ...  Pillai, R. S., Bhattacharyya, S. N., Artus, C.  ... 
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