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Chemical Imaging of Poplar Wood Cell Walls by Confocal Raman Microscopy

N. Gierlinger
2006 Plant Physiology  
Confocal Raman microscopy was used to illustrate changes of molecular composition in secondary plant cell wall tissues of poplar (Populus nigra 3 Populus deltoids) wood.  ...  Scanning Raman microscopy was thus shown to be a powerful, nondestructive tool for imaging changes in molecular cell wall organization with high spatial resolution.  ...  While Raman scattering involves excitation of a molecule by inelastic scattering with a photon (from a laser light source), infrared absorption spectroscopy typically involves photon absorption, with the  ... 
doi:10.1104/pp.105.066993 pmid:16489138 pmcid:PMC1435827 fatcat:rqbx34y5ybayznyjuqhnqntuiu

Light-Sheet Scattering Microscopy to Visualize Long-Term Interactions Between Cells and Extracellular Matrix

Xiangda Zhou, Renping Zhao, Archana K. Yanamandra, Markus Hoth, Bin Qu
2022 Frontiers in Immunology  
Here, we combine label-free light-sheet scattering microcopy (LSSM) and fluorescence microscopy to solve these problems.  ...  The quality and intensity of collagen structure images acquired by LSSM did not decline with time.  ...  In comparison, images taken by confocal reflection microscopy are formed from the light reflected by the collagen fibers.  ... 
doi:10.3389/fimmu.2022.828634 pmid:35154150 pmcid:PMC8831865 fatcat:d3ooh6bo2za7ffl3jy6rpqsjve

Migration of highly aggressive MV3 melanoma cells in 3-dimensional collagen lattices results in local matrix reorganization and shedding of alpha2 and beta1 integrins and CD44

P Friedl, K Maaser, C E Klein, B Niggemann, G Krohne, K S Zänker
1997 Cancer Research  
from unfixed and fixed samples by confocal reflection contrast in combination with immunofluorescence staining.  ...  reflection imaging with immunofluorescence.  ...  fixed, stained for /31 integrins, and retrieved by confocal microscopy.  ... 
pmid:9158006 fatcat:dcl5qs2pirbvbf3335e75jgkpu

Loss of the small heat shock protein αA-crystallin does not lead to detectable defects in early zebrafish lens development

Mason Posner, Jackie Skiba, Mary Brown, Jennifer O. Liang, Justin Nussbaum, Heather Prior
2013 Experimental Eye Research  
microscopy.  ...  Our demonstration that lens cataracts can be visualized in three-dimensions by confocal microscopy in a living zebrafish provides a new tool for studying the causes, development and prevention of lens  ...  and imaged by confocal microscopy to detect light scattering in the lens.  ... 
doi:10.1016/j.exer.2013.09.007 pmid:24076322 pmcid:PMC3864005 fatcat:2vbpsqmhpjgpzmr4dgswod6eka

Spatiotemporal heterogeneity of κ-carrageenan gels investigated via single-particle-tracking fluorescence microscopy

Koen Martens, John van Duynhoven, Johannes Hohlbein
2020 Langmuir  
Here, we use single-particle-tracking fluorescence microscopy (sptFM) to quantitatively describe κ-carrageenan gels.  ...  In the dense network regions, we found a fraction with a largely decreased diffusion coefficient of ~1∙10 13 m2/s. We also observed dynamic exchange between these states.  ...  . 30 Occasionally, the emission light was guided via the bypass mode of a rescan confocal microscope, which reduced the pixel size to 122 nm (at 2 × 2 pixel binning).  ... 
doi:10.1021/acs.langmuir.0c00393 pmid:32343144 pmcid:PMC7254830 fatcat:dmz7wpe4ebfdzgjj77ntesvrtu

Mechanotransduction of mesenchymal melanoma cell invasion into 3D collagen lattices: Filopod-mediated extension–relaxation cycles and force anisotropy

Josefine Starke, Kerstin Maaser, Bernhard Wehrle-Haller, Peter Friedl
2013 Experimental Cell Research  
Using high-resolution 3D confocal reflectance and fluorescence microscopy in GFP/actin expressing melanoma cells, we here employ time-resolved subcellular coregistration of cell morphology, interaction  ...  and alignment of actin-rich protrusions engaged with individual collagen fibrils.  ...  The data further show a mode of cell migration driven by a single dominant protrusion, with directionally persistent track generation as outcome.  ... 
doi:10.1016/j.yexcr.2013.04.003 pmid:23830878 fatcat:ta4b3agz2fcqrhewruopyrfuhe

Microvesicles released from tumor cells disrupt epithelial cell morphology and contractility

Francois Bordeleau, Bryan Chan, Marc A. Antonyak, Marsha C. Lampi, Richard A. Cerione, Cynthia A. Reinhart-King
2016 Journal of Biomechanics  
During tumor progression, cancer cells interact and communicate with non-malignant cells within their local microenvironment.  ...  Furthermore, we show that this ECM reorganization is associated with an increase in cellular traction force following MV treatment, higher acto-myosin contractility, and higher FAK activity.  ...  A solid state 405 nm laser illuminated the gel and backscattered light from collagen fibers was captured through a 32-mm pinhole, giving 1mm thick confocal slices.  ... 
doi:10.1016/j.jbiomech.2015.10.003 pmid:26477404 pmcid:PMC4826648 fatcat:xryrr6vxcbfuvjitjb2hfffmae

Hybrid Capillary-Microfluidic Device for the Separation, Lysis, and Electrochemical Detection of Vesicles

Donna M. Omiatek, Michael F. Santillo, Michael L. Heien, Andrew G. Ewing
2009 Analytical Chemistry  
These results were compared to an orthogonal size measurement using dynamic light scattering to validate the detection platform.  ...  The effect of sheath-flow on analyte dispersion was characterized using confocal fluorescence microscopy and electrochemical detection.  ...  Dynamic Light Scattering A Zetasizer Nano S (Malvern Instruments, Worcestershire, UK) was used to acquire light scattering data.  ... 
doi:10.1021/ac802466g pmid:19228035 pmcid:PMC2656409 fatcat:dxcsr6lvonhcbayikyxtwrjuiy

Walking the Line: A Fibronectin Fiber-Guided Assay to Probe Early Steps of (Lymph)angiogenesis

Maria Mitsi, Martin Michael Peter Schulz, Epameinondas Gousopoulos, Alexandra Michaela Ochsenbein, Michael Detmar, Viola Vogel, Christina Lynn Addison
2015 PLoS ONE  
To address such questions, we introduced here an in vitro (lymph)angiogenesis assay, where we used microbeads coated with endothelial cells as simple sprouting sources and deposited them on single Fn fibers  ...  The fibers were deposited on a transparent substrate, suitable for live microscopic observation of the ensuing cell outgrowth events at the single cell level.  ...  Tobias Schwarz and the ETH Zurich light microcopy center (LMC) for help with the CellR microscope.  ... 
doi:10.1371/journal.pone.0145210 pmid:26689200 pmcid:PMC4686943 fatcat:5peyg5tbefcctkk2q6ums3b2n4

α2-Adrenergic receptor binding in canine Purkinje fibers

Hon-Chi Lee, Ricardo A. Samson, John J. Cai
1996 FEBS Letters  
Membrane fractions showed high affinity, saturable, and displaceable binding with [3H]yohimbine in preparations from canine cardiac Purkinje fibers but not those from canine cardiac ventricnlar myocardium  ...  Scatchard analysis of the canine Purkinje membrane a2-adrenergic receptor binding showed a Bmax of 54.9 fmol/mg protein with a K d of 6.25 nM.  ...  Acknowledgements: We would like to thank Thomas Monninger of the Central Electron Microscopy Research Facility, University of Iowa, for his expert technical assistance.  ... 
doi:10.1016/0014-5793(96)00002-6 pmid:8603743 fatcat:36whxgz7u5clvcfg2wjmhnpe3a

Compensation mechanism in tumor cell migration

Katarina Wolf, Irina Mazo, Harry Leung, Katharina Engelke, Ulrich H. von Andrian, Elena I. Deryugina, Alex Y. Strongin, Eva-B. Bröcker, Peter Friedl
2003 Journal of Cell Biology  
Acquisition of protease-independent amoeboid dissemination was confirmed for HT-1080 cells injected into the mouse dermis monitored by intravital multiphoton microscopy.  ...  change, i.e., propulsive squeezing through preexisting matrix gaps and formation of constriction rings in the absence of matrix degradation, concomitant loss of clustered ␤ 1 integrins and MT1-MMP from fiber  ...  Fiber breakdown was detected after 40 h by Coomassie blue staining and confocal backscatter microscopy.  ... 
doi:10.1083/jcb.200209006 pmid:12527751 pmcid:PMC2172637 fatcat:ftckyhr67bdodl6czawmzpfxee

Depletion-Driven Morphological Control of Bundled Actin Networks [article]

James Clarke
2022 arXiv   pre-print
Actin is known to undergo a phase transition from a single-filament state to a bundled state by the addition of polyethylene glycol (PEG) molecules in sufficient concentration.  ...  DMR-1720595 with additional support from the Welch Foundation (F-1848).  ...  or light scattering.  ... 
arXiv:2205.01864v1 fatcat:sqoe4enpzzbqbj5to7qfszenfu

Comparative mechanisms of cancer cell migration through 3D matrix and physiological microtracks

Shawn P. Carey, Aniqua Rahman, Casey M. Kraning-Rush, Bethsabe Romero, Sahana Somasegar, Olivia M. Torre, Rebecca M. Williams, Cynthia A. Reinhart-King
2015 American Journal of Physiology - Cell Physiology  
Since confocal reflectance microscopy depends on interfacial light scattering due to refractive index mismatch, lack of reflectance signal indicates a lack of light-scattering material within the aqueous  ...  As shown by confocal reflectance microscopy, all matrices were composed of interconnected networks of collagen fibers and pores, the structure and organization of which were concentration-dependent: matrices  ... 
doi:10.1152/ajpcell.00225.2014 pmid:25500742 pmcid:PMC4360026 fatcat:5f7hqliltvh2rmw5hne5xssg7m

Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers

Marcelo J. Salierno, Luis García-Fernandez, Noelia Carabelos, Karin Kiefer, Andrés J. García, Aránzazu del Campo
2016 Biomaterials  
The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand.  ...  Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers.  ...  Surface patterns were then imaged by confocal microscopy (Leica TCS SP5) and analysed.  ... 
doi:10.1016/j.biomaterials.2015.12.001 pmid:26757258 fatcat:cp74yifnrvhydl42w4y2bje4ou

Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves

Bartomeu Colom, Yannick Poitelon, Wenlong Huang, Abigail Woodfin, Sharon Averill, Ubaldo Del Carro, Desirée Zambroni, Susan D. Brain, Mauro Perretti, Amrita Ahluwalia, John V. Priestley, Triantafyllos Chavakis (+3 others)
2012 The FASEB Journal  
The study also reports on previously undetected expressions of JAM-C, namely on perineural cells, and in line with nociception defects of the JAM-C SC KO animals, on finely myelinated sensory nerve fibers  ...  To investigate the role of JAM-C in neuronal functions further, mice with a specific deletion of JAM-C in SCs (JAM-C SC KO) were generated.  ...  E, F) Average length of the node of Ranvier was quantified from WT and JAM-C SC KO mouse sciatic nerves by EM (E; nϭ19 and 30 nodes, respectively, from 3 mice/group) and by confocal microscopy (F) with  ... 
doi:10.1096/fj.11-196220 pmid:22090315 pmcid:PMC3370675 fatcat:4c3a52lu5neehangh4kgczl3em
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