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Cancer Proteomics: The State of the Art

Paul C. Herrmann, Lance A. Liotta, Emanuel F. Petricoin III
2001 Disease Markers  
The study of proteomics should yield an unparalleled understanding of cancer as well as an invaluable new target for therapeutic intervention and markers for early detection.  ...  Now that the human genome has been determined, the field of proteomics is ramping up to tackle the vast protein networks that both control and are controlled by the information encoded by the genome.  ...  Currently, the predominant technique for protein purification and separation in proteomics is currently two-dimensional (2D) gel electrophoresis (Fig. 3) .  ... 
doi:10.1155/2001/327047 pmid:11673651 pmcid:PMC3851073 fatcat:taejxfamkncdhnqvrdcbcewwtq

State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks

Haijiao Wang, Song Song, Huaqiang Cheng, Yan-Wen Tan
2020 International Journal of Molecular Sciences  
Herein, we would like to summarize the identification and improvement of several representative brassinosteroid signaling components through the development of new technologies, including brassinosteroid-insensitive  ...  studies.  ...  Two-dimensional difference gel electrophoresis (2D-DIGE) is a modified version of 2D-PAGE. In 2D-DIGE, different protein samples are labeled with different fluorescent tags [33] .  ... 
doi:10.3390/ijms21218179 pmid:33142942 pmcid:PMC7662629 fatcat:p3eae4omangwpizpwk4i6msgky

Current state of the art for enhancing urine biomarker discovery

Michael Harpole, Justin Davis, Virginia Espina
2016 Espert Review of Proteomics  
Advancements in mass spectrometry based urinary proteomics The ever-increasing depth and breadth of the urinary proteome is attributable to the versatility of mass spectrometry for protein discovery, identification  ...  High-resolution mass spectrometry, coupled with state of the art fractionation systems are revealing the plethora of diagnostic/prognostic proteomic information existing within urinary exosomes, glycoproteins  ...  The funding sources did not have any role in the study design; the collection, analysis and interpretation of data; manuscript preparation; or the decision to submit the paper for publication.  ... 
doi:10.1080/14789450.2016.1190651 pmid:27232439 pmcid:PMC4978532 fatcat:3h6zhzwgvfcdvp4ynsgpwhuqpy

Proteomics: State of the Art and Its Relevance for Gene Therapy [chapter]

Sergiu Scobioala, Rainer Klocke, Günter Michel, Sigrid Nikol
2006 Cardiovascular Research  
We thank the Interdisciplinary Center for Clinical Research (IZKF, project B15) for supporting Dr Sergiu Scobioala.  ...  examples of annotated protein and two-dimensional electrophoresis databases (Fig. 6 ).  ...  Traditionally, proteomic analyses or "proteomics" means the separation of a large number of proteins from a cell line or organism at a given time point in 2-dimensional polyacrylamide gel electrophoresis  ... 
doi:10.1007/0-387-23329-6_2 fatcat:s3rnk4grqzeo5i6wgouwwnvkgu

Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions [chapter]

Marco Chiapello, Silvia Perotto, Raffaella Balestrini
2015 Recent Advances in Proteomics Research  
In this chapter, we focus on the use of proteomic tools to better understand the molecular bases of cell communication and the regulation of developmental and metabolic pathways in mycorrhizal associations  ...  the establishment and functioning of these symbioses.  ...  They used two-dimensional electrophoresis (2DE)-PAGE, a gel electrophoresis technique introduced in the middle 1970s by O'Farrell and Klose and able to separate with high-resolution proteins by two orthogonal  ... 
doi:10.5772/61331 fatcat:vpmamy243zbxlmx7k5g2klfbnu

State of the Art in Tumor Antigen and Biomarker Discovery

Klervi Even-Desrumeaux, Daniel Baty, Patrick Chames
2011 Cancers  
Our knowledge of tumor immunology has resulted in multiple approaches for the treatment of cancer.  ...  The challenge is now to discover new targets for active and passive immunotherapy.  ...  -051-01 and by the ARC (Association pour la Recherche contre le Cancer).  ... 
doi:10.3390/cancers3022554 pmid:24212823 pmcid:PMC3757432 fatcat:bevan7iv55fpze5hdgbudqj5te

Integrating Pharmacoproteomics into Early-Phase Clinical Development: State-of-the-Art, Challenges, and Recommendations

Savita Nandal, Tal Burt
2017 International Journal of Molecular Sciences  
, and select proteins, or to the proteome as a whole.  ...  We review the history, technologies, current usage, challenges, and potential for future use, and conclude with recommendations for integration of pharmacoproteomic in early-phase drug development.  ...  Author Contributions: Savita Nandal and Tal Burt analyzed and reviewed the data. Savita Nandal and Tal Burt authors wrote different sections of the paper.  ... 
doi:10.3390/ijms18020448 pmid:28218733 pmcid:PMC5343982 fatcat:xptmrwbqtjgc5doh6bfr27obda

Protein Array Technology

Konrad B??ssow, Zolt??n Konthur, Angelika Lueking, Hans Lehrach, Gerald Walter
2001 American Journal of Pharmacogenomics  
The chapter describes the pitfalls and challenges in Protein Microarray technology to produce native and functional proteins and store them in a native and special environment for every single spot on  ...  Outside of a narrow range of environmental conditions, proteins will denature, lose their three-dimensional structure and a lot of their specificity and activity.  ...  From 20 Electrophoresis and Microtitre Plates to Microarrays of Biomolecules Two-dimensional gel electrophoresis separates proteins according to size and charge, therefore allowing the study of cell, tissue  ... 
doi:10.2165/00129785-200101010-00005 pmid:12173313 fatcat:5hkarcluufb4xirx5wo4kwcmxu

Clinical Application of Proteomics in Breast Cancer: State of the Art and Perspectives

Anthony Goncalves, François Bertucci
2011 Medical Principles and Practice  
Application of proteomics-based techniques is also considered crucial for detecting new biomarkers.  ...  In this review, we present the proteomics-based methods that have been applied to date to breast cancer samples for diagnostic and prognostic purposes.  ...  Principles of two-dimensional differential gel electrophoresis.  ... 
doi:10.1159/000319544 pmid:21160207 fatcat:nec6stq4nra4rml2gpb36ynk3u

Proteomic Technologies for the Study of Osteosarcoma

Stephanie D. Byrum, Charity L. Washam, Corey O. Montgomery, Alan J. Tackett, Larry J. Suva
2012 Sarcoma  
In this paper, we focus on the growing list of available state-of-the-art proteomic technologies and their specific application to the discovery of novel osteosarcoma diagnostic and therapeutic targets  ...  The mechanism of osteosarcoma tumorogenesis is poorly understood, and few proteomic studies have been used to interrogate the disease thus far.  ...  Acknowledgments The authors current research efforts are supported by the NIH R01DA025755 (AJT), NIH F32GM093614 (SDB), and the Arkansas Breast Cancer Research Program at the University of Arkansas for  ... 
doi:10.1155/2012/169416 pmid:22550414 pmcid:PMC3329661 fatcat:u6w35frmzrf2hi7hjv5fwmizmq

The state of the art in the analysis of two-dimensional gel electrophoresis images

Matthias Berth, Frank Michael Moser, Markus Kolbe, Jörg Bernhardt
2007 Applied Microbiology and Biotechnology  
Software-based image analysis is a crucial step in the biological interpretation of two-dimensional gel electrophoresis experiments.  ...  We close with an overview of visualization and presentation methods (proteome maps) and current challenges in the field.  ...  Acknowledgment We are thankful to Julia Bandow (Pfizer) for crucial input to the development of the 2-D gel analysis workflow based on consensus spot patterns (Bandow et al. 2007) .  ... 
doi:10.1007/s00253-007-1128-0 pmid:17713763 pmcid:PMC2279157 fatcat:hocmhtfr6bft3op6cmry4awrlm

The Schistosoma gene discovery program: state of the art

Glória R. Franco, Analina F. Valadão, Vasco Azevedo, Élida M.L. Rabelo
2000 International Journal of Parasitology  
One of the main objectives of this program is the discovery and characterisation of new genes of Schistosoma mansoni and Schistosoma japonicum in an attempt to search for new targets for drugs and vaccine  ...  Gaining knowledge about the genome of these parasites is of importance for the understanding of their biology, mechanisms of drug resistance and antigenic variation that determine escape from the host's  ...  Acknowledgements The authors wish to thank all members of the WHO Schistosoma Genome Network for the integrated eort for the production of all data.  ... 
doi:10.1016/s0020-7519(00)00020-5 pmid:10731568 fatcat:qyeeghe6trg37nruxt4q7a6pmm

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

A Pielaat, GC Barker, P Hendriksen, P Hollman, A Peijnenburg, BH Ter Kuile
2013 EFSA Supporting Publications  
A foresight study on emerging technologies: State of the art of omics technologies and potential applications in food and feed safety Pielaat, A.; Barker, G.C.; Hendriksen, P.; Peijnenburg, A.; ter Kuile  ...  For this, two-dimensional gel electrophoresis coupled with mass spectrometry was used.  ...  Examples of proteomics techniques are two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) for protein separation and identification, respectively .  ... 
doi:10.2903/sp.efsa.2013.en-495 fatcat:p62tnrhkuzfmpewkoukfjc3fba

PROTEOMICS TECHNOLOGY AND THERAPEUTICS

Michelle Li Wen Hong, Nan Jiang, Sravanthy Gopinath, Fook Tim Chew
2006 Clinical and Experimental Pharmacology and Physiology  
These include the two-dimensional gel electrophoresis, liquid chromatography, mass spectrometry, isotope-coded affinity tag and protein biochips. 3.  ...  Proteins are the workhorses of biological systems and, thus, it is not surprising that the field of proteomics has flourished in recent years.  ...  Two-dimensional gel electrophoresis Two-dimensional gel electrophoresis is the oldest approach and the most powerful protein separation method available today, providing parallel investigation of up to  ... 
doi:10.1111/j.1440-1681.2006.04407.x pmid:16700895 fatcat:ggcrag7e3baufibfiia763jbbm

The state of the art of in vitro fertilization

Adam J Fechner
2011 Frontiers in Bioscience (Elite Edition)  
Proteomics/Secretomics Earlier proteomic research in mouse embryos involved the use of two-dimensional gel electrophoresis (54), Western blot (55), or enzyme-linked immunosorbent assay (56) to identify  ...  More recently, the use of mass spectrometry for embryonic proteomics has enabled the simultaneous identification of numerous proteins, representing a significant advance in the field.  ... 
doi:10.2741/e242 pmid:21196307 fatcat:ogaippocu5dqdlo3dqamvkzjjm
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