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Prediction of Myotoxic and Neurotoxic Activities in Phospholipases A2 from Primary Sequence Analysis [chapter]

Fabiano Pazzini, Fernanda Oliveira, Jorge A. Guimarães, Hermes Luís Neubauer de Amorim
2005 Lecture Notes in Computer Science  
We developed a methodology to predict myotoxicity and neurotoxicity of proteins of the family of Phospholipases A2 (PLA2) from sequence data.  ...  We have used the results of this work to build a web tool (available at www.cbiot.ufrgs.br/bioinfo/phospholipase) to classify PLA2s of unknown function regarding myotoxic or neurotoxic activity.  ...  We developed a methodology to predict myotoxicity and neurotoxicity of proteins of the family of Phospholipases A2 (PLA2) from sequence data.  ... 
doi:10.1007/11532323_21 fatcat:ijuy2sawtnc3lpt7qpxoiezn6m

A Novel Phospholipase A2 Isolated from Palythoa caribaeorum Possesses Neurotoxic Activity

Miguel Cuevas-Cruz, Fernando Lazcano-Pérez, Ulises Hernández-Guzmán, Karen Helena Díaz de la Vega-Castañeda, Sergio A. Román-González, Norma A. Valdez-Cruz, Benjamín Velasco‐Bejarano, Ana Laura Colín-González, Abel Santamaría, Saúl Gómez-Manzo, Jaime Marcial-Quino, Roberto Arreguín-Espinosa
2019 Toxins  
The analysis of the A2-PLTX-Pcb1a sequence showed neurotoxic domain similitude with other neurotoxic sPLA2´s, but a different catalytic histidine domain.  ...  This work was aimed at isolating and functionally characterizing a cnidarian neurotoxic phospholipase named A2-PLTX-Pcb1a for the first time.  ...  Eréndira García Ríos from IQ-UNAM for technical assistance, and Ricardo González Muñoz for his help during specimen collection. Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/toxins11020089 fatcat:evvneor4dzcrho2yx2b2dreynu

Neurotoxicity and Other Pharmacological Activities of the Snake Venom Phospholipase A2OS2: The N-Terminal Region Is More Important Than Enzymatic Activity†

Morgane Rouault, Lachlan D. Rash, Pierre Escoubas, Eric Boilard, James Bollinger, Bruno Lomonte, Thomas Maurin, Carole Guillaume, Stéphane Canaan, Christiane Deregnaucourt, Joseph Schrével, Alain Doglio (+4 others)
2006 Biochemistry  
Several snake venom secreted phospholipases A 2 (sPLA 2 s) including OS 2 exert a variety of pharmacological effects ranging from central neurotoxicity to anti-HIV activity by mechanisms that are not yet  ...  To conclusively address the role of enzymatic activity and map the key structural elements of OS 2 responsible for its pharmacological properties, we have prepared single point  ...  Acknowledgment We thank Catherine Le Calvez, Sabine Scarzello, and Romain Gauthier for expert assistance in the structural characterization and computer modeling of the OS 2 protein.  ... 
doi:10.1021/bi060217r pmid:16669624 pmcid:PMC2796912 fatcat:hkxf4whog5cvddelkzygt2dkri

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

Nur Atiqah Haizum Abdullah, Muhamad Rusdi Ahmad Rusmili, Syafiq Asnawi Zainal Abidin, Mohd Farooq Shaikh, Wayne C. Hodgson, Iekhsan Othman
2021 Toxins  
Based on LC-MSMS, A2-EPTX-Nsm1a was found to show high sequence similarity with PLA2 from venoms of other Naja species. The PLA2 activity of A2-EPTX-Nsm1 was inhibited by 4-BPB and EDTA.  ...  Phospholipase A2 (PLA2) toxins are one of the main toxin families found in snake venom.  ...  Acknowledgments: We would like to thank Nurziana Sharmilla and Nasiha Musa for their technical support and Zainuddin Ismail for donating N. sumatrana venom from his private collection.  ... 
doi:10.3390/toxins13120859 pmid:34941697 pmcid:PMC8709200 fatcat:fzoudg3u3vfj3d7g56lwihlsii

Bothrops moojeni myotoxin-II, a Lys49-phospholipase A2 homologue: An example of function versatility of snake venom proteins

Rodrigo G. Stábeli, Saulo F. Amui, Carolina D. Sant'Ana, Matheus G. Pires, Auro Nomizo, Marta C. Monteiro, Pedro R.T. Romão, Renata Guerra-Sá, Carlos A. Vieira, José R. Giglio, Marcos R.M. Fontes, Andreimar M. Soares
2006 Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology  
: cDNA sequence and molecular structure prediction.  ...  sites" distinct from the catalytic site in snake venom myotoxic PLA 2 s.  ...  Acknowledgements The authors express their gratitude to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and  ... 
doi:10.1016/j.cbpc.2005.11.020 pmid:16442348 fatcat:hk3ib4i3vjedvnsjgfovhzkexm

Novel acidic phospholipase A2 from Porthidium hyoprora causes inflammation with mast cell rich infiltrate

Petrus Pires Marques, Alessandra Esteves, Marcelo Lancellotti, Luis Alberto Ponce-Soto, Sergio Marangoni
2015 Biochemistry and Biophysics Reports  
Phospholipases A2 (PLA2) are a group of enzymes that hydrolyze phospholipids at the sn-2 position, being present in all nature.  ...  Structural studies using mass spectrometry confirmed an acidic secretory PLA2, family IIA, with molecular mass of 13,620.9 Da and identification of 86% of its primary sequence.  ...  Despite of its activity, the acidic PLA 2 usually shows weak neurotoxicity, myotoxicity and lethal potency in vivo, but induces other significant pharmacological effects such as inhibition of platelet  ... 
doi:10.1016/j.bbrep.2015.03.001 pmid:29124136 pmcid:PMC5668520 fatcat:oj6zmrbqqnbttkg5psypmeiuaq

cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

F.H.R. Fagundes, M. Oliveira, S. Huancahuire-Vega, F.F. Romero-Vargas, L.A. Ponce-Soto, S. Marangoni
2010 Brazilian Journal of Medical and Biological Research  
Baldasso and Marino Forner for general technical help and Granja Ito S/A (Sumaré, SP, Brazil) for supplying male, 4-8-day-old HY-LINE W36 chickens (Gallus gallus).  ...  (3) for the comparison of its deduced molecular structure with its primary structure and with its neurotoxic activity.  ...  This toxin is responsible for myotoxic and neurotoxic activities and in this rattlesnake species (C. durissus terrificus) it accounts for 20% of the dry venom.  ... 
doi:10.1590/s0100-879x2010007500009 pmid:20401434 fatcat:uxnittrz6fb7fk7zpcxnsfi7ja

Structural and functional characterization of myotoxin I, a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops atrox

Vitelbina Núñez, Viviana Arce, José Marı́a Gutiérrez, Bruno Lomonte
2004 Toxicon  
Accordingly, the toxin was devoid of phospholipase and anticoagulant activities, in vitro. In mice, it induced conspicuous local myonecrosis, edema, and a systemic interleukin-6 response.  ...  A new myotoxin was isolated from the venom of Bothrops atrox from Colombia. B. atrox myotoxin I is a homodimer, with a subunit molecular mass of 13,826, and a pI of 8.9.  ...  Nú nez, and the financial support from Vicerrectoría de Investigación-UCR (VI-741-99269), the Embassy of Japan in Costa Rica, and UNESCO (883.701-3), are also acknowledged.  ... 
doi:10.1016/j.toxicon.2004.04.013 pmid:15225567 fatcat:hqce4jehjrcbvlajkoi7up5mjy

Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins

Vincent Louis Viala, Diana Hildebrand, Tamara Mieco Fucase, Juliana Mozer Sciani, José Pedro Prezotto-Neto, Maria Riedner, Leonardo Sanches, Paula Juliana Nishimura, Nancy Oguiura, Daniel Carvalho Pimenta, Hartmut Schlüter, Christian Betzel (+2 others)
2015 Toxicon  
mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein species based on unique peptide analysis and sequence database enriched with species-specific venom  ...  Our results point to a broad arsenal of toxins in C. vegrandis venom: PIII and PII metalloproteases, crotoxin subunits, other phospholipases, isoforms of serine proteases and lectins, L-amino-acid oxidase  ...  sequencing; grant and scholarship from FAPESP (SP, Brazil) (2010/08585-8 and 2009/10305-8) and CAPES/DAAD no 364/11 (Brazil/Germany research program).  ... 
doi:10.1016/j.toxicon.2015.09.023 pmid:26403864 fatcat:gawo5nobqbdylnaiys5y7ailua

Structural and Functional Characterization of BnSP-7, a Lys49 Myotoxic Phospholipase A2 Homologue from Bothrops neuwiedi pauloensis Venom

Andreimar M Soares, Renata Guerra-Sá, Caroline R Borja-Oliveira, Veridiana M Rodrigues, Léa Rodrigues-Simioni, Vanderlei Rodrigues, Marcos R.M Fontes, Bruno Lomonte, José M Gutiérrez, José R Giglio
2000 Archives of Biochemistry and Biophysics  
In addition to its in vivo myotoxic and edema-inducing activity, it disrupted artificial membranes.  ...  BnSP-7, a Lys49 myotoxic phospholipase A 2 homologue from Bothrops neuwiedi pauloensis venom, was structurally and functionally characterized.  ...  In addition to their primary catalytic activity, many class I and II PLA 2 s from snake venoms exhibit several pharmacological effects, including myotoxicity, edema formation, pre-and postsynaptic neurotoxicity  ... 
doi:10.1006/abbi.2000.1790 pmid:10860537 fatcat:k4zrj7vevvhxvmohdagp2dn7mm

Short Linear Motifs Characterizing Snake Venom and Mammalian Phospholipases A2

Caterina Peggion, Fiorella Tonello
2021 Toxins  
Snake venom phospholipases A2 (PLA2s) have sequences and structures very similar to those of mammalian group I and II secretory PLA2s, but they possess many toxic properties, ranging from the inhibition  ...  Here, we compare sequence alignments of snake venom and mammalian PLA2s, grouped according to their structure and biological activity, looking for differences that can justify their different behavior.  ...  Acknowledgments: The authors acknowledge "The animal toxin annotation project" part of the Swiss-Prot database organization, in which proteins secreted in animal venom are systematically annotated.  ... 
doi:10.3390/toxins13040290 pmid:33923919 fatcat:pookh2lur5eydpaqtjn4tyybrq

Isolation and Characterization of Mucilage from Cinnamon verum

E. Vadivel, Poorva P. Dabholkar
2018 International Journal of Pharmaceutical Sciences and Drug Research  
In the present study, mucilage was isolated from the bark of Cinnamon verum.  ...  Extracted mucilage swells in water and slightly soluble in organic solvents. The preliminary phytoconstituents studies showed the presence of carbohydrates, mucilage and phenolic compounds.  ...  Myricetin-3-O-rhamnoside (C21H20O12 Also induce neurotoxicity, cardiotoxicity and myotoxicity.  ... 
doi:10.25004/ijpsdr.2018.100403 fatcat:rjv6nm66h5ggrc2xdj3emnnzwm

A Novel Phospholipase A2(D49) from the Venom of theCrotalus oreganus abyssus(North American Grand Canyon Rattlesnake)

W. Martins, P. A. Baldasso, K. M. Honório, V. G. Maltarollo, R. I. M. A. Ribeiro, B. M. A. Carvalho, A. M. Soares, L. A. Calderon, R. G. Stábeli, M. A. O. Caballol, G. Acosta, E. Oliveira (+3 others)
2014 BioMed Research International  
The analysis of a product from cDNA (E6d), derived from the gland of a specieCrotalus viridis viridis, was found to produce an acid phospholipase A2.  ...  Our studies show that the PLA2produced from the cDNA ofCrotalus viridis viridis(named E6d) is exactly the same PLA2primary sequence of amino acids isolated from the venom ofCrotalus oreganus abyssus.  ...  ) analysis of the influence of calcium ions and other divalent cations on the phospholipase A 2 activity of CoaPLA 2 .  ... 
doi:10.1155/2014/654170 pmid:24707493 pmcid:PMC3953673 fatcat:jpntrh4rp5f7jowa4zdbfecz2u

Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

L. St Pierre, R. Woods, S. Earl, P. P. Masci, M. F. Lavin
2005 Cellular and Molecular Life Sciences (CMLS)  
The neurotoxic, haemotoxic and myotoxic effects of Australian elapid envenomation are largely the result of the activity of a myriad of neurotoxins and phospholipase A, (PLA,;) enzymes within the venom  ...  and myotoxic activity, respectively, with variable phospholipase enzymatic activity [24, 47].  ... 
doi:10.1007/s00018-005-5384-9 pmid:16261251 fatcat:riqoqokezzeepmr4uw23lbknoq

Molecular cloning and characterization of a neurotoxic phospholipase A2from the venom of Taiwan habu (Trimeresurus mucrosquamatus)

I H Tsai, P J Lu, Y M Wang, C L Ho, L L Liaw
1995 Biochemical Journal  
Its complete primary structure was solved by an automated N-terminal sequencing and cDNA sequencing method.  ...  Trimucrotoxin exists mainly as a homodimer of 14 kDa subunits as shown by a gel-filtration experiment, and dissociates into monomers during SDS/PAGE in the absence of Ca2l. However,  ...  Yen for the gift of crotoxin A, Dr. F. M. Pan for assistance in preparing the cDNA, and Mrs. L. L. Huang and C. C. Tseng for some neurotoxicity assays.  ... 
doi:10.1042/bj3110895 pmid:7487947 pmcid:PMC1136085 fatcat:jqm6eqrct5byxfluaypehkwuwq
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