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Nucleosome-nucleosome interaction in chromatin

V.A. Pospelov, S.B. Svetlikova, V.I. Vorob'ev
1979 FEBS Letters  
In this work we used an enzymatic approach to the study of nucleosome packing in chromatin.  ...  In the presence of divalent metal ions nucleosomes have been shown tightly packed in chromatin [3, 4] .  ... 
doi:10.1016/0014-5793(79)80263-x pmid:374121 fatcat:s55zhql4jbhlbfnutqx5m7o4au

Alignment of nucleosomes along DNA and organization of spacer DNA inDrosophilachromatin

V.L. Karpov, S.G. Bavykin, O.V. Preobrazhenskaya, A.V. Belyavsky, A.D. Mirzabekov
1982 Nucleic Acids Research  
A series of mono-and dinucleosomal DNAs characterized by an about ten-base periodicity in the size were revealed in the micrococcal nuclease digests of Drosophila chromatin which have 180-5 base pair (  ...  bp) nucleosomal repeat. 20, 30, and 40 bp spacers were found to be predominant in chromatin by trimming DNA in dinucleosomes to the core position.  ...  IB, b) as well as A regular series of DNA fragments in the micrococcal nuclease digests of chromatin (A) or nuclei (B).  ... 
doi:10.1093/nar/10.14.4321 pmid:6812025 pmcid:PMC320802 fatcat:kl37ajfvlbaojkm7jldwv5go3m

On the mechanism of nucleodisome splitting off by nucleases

V.A. Pospelov, S.B. Svetlikova
1982 FEBS Letters  
Takhtadjan, for assistance in the performing of some experiments.  ...  It seems that, besides a specific nucleosome packing in chromatin, the accessibility of certain sites of the nucleosomal DNA to nucleases can influence the formation of a doublenucleosome repeat.  ...  The modes of DNA fragmentation in chromatin by these nucleases are distinguished by at least one feature: micrococcal nuclease preferentially cleaves the linker DNA, and DNase I effectively breaks the  ... 
doi:10.1016/0014-5793(82)80725-4 pmid:6216116 fatcat:tkd4mwaph5hczkziincldh5ma4

Chromatin structures: dissecting their mixed patterns in nuclease digests

Roger D. Drinkwater, Peter J. Wilson, Jennifer D. Skinner, Leigh A. Burgoyne
1987 Nucleic Acids Research  
Four separate features could be distinguished in Fe-DNAase-1 digestions of human lymphoblast nuclei: a di-nucleosomal (2N) repeat, a mononucleosomal (iN) repeat, a component of "random" DNA, and triple  ...  Hybridization studies indicated that the centromeric classes of repetitive DNA had the same digestion spectra as the major interspersed classes of repetitive DNA, and DNA enriched in transcriptionally  ...  ACKNOWLEDGEMENTS This work was supported by the Australian Research Grants Scheme.  ... 
doi:10.1093/nar/15.19.8087 pmid:3671072 pmcid:PMC306328 fatcat:jsvjvbxhvrgkbgwguvoahpilbq

High-Resolution Structural Analysis of Chromatin at Specific Loci:Saccharomyces cerevisiaeSilent Mating-Type LocusHMRa

Anish Ravindra, Kerstin Weiss, Robert T. Simpson
1999 Molecular and Cellular Biology  
The nucleosomes are arranged in pairs with very short linkers; the pairs are separated from one another by longer linkers of ∼20 bp.  ...  With high-resolution micrococcal nuclease mapping, we show that theHMRa locus has 12 precisely positioned nucleosomes spanning the distance between the E and I silencer elements.  ...  This work was supported by a grant from the National Institute of General Medical Sciences, National Institutes of Health, GM52311.  ... 
doi:10.1128/mcb.19.12.7944 pmid:10567520 pmcid:PMC84879 fatcat:uemdeigvlrezji2hotabzzesvi

Differences of supranucleosomal organization in different kinds of chromatin: cell type-specific globular subunits containing different numbers of nucleosomes

H Zentgraf
1984 Journal of Cell Biology  
Fractions of homogeneously-sized supranucleosomal particles can be obtained in high yield and purity from various types of cells by brief micrococcal nuclease digestion (10 or 20 s) of condensed chromatin  ...  These resembled the unit chromatin fibrils fixed in situ, which contain an average of 48 nucleosomes, as determined both by electron microscopy after unraveling in low salt buffer and gel electrophoresis  ...  Additionally chicken erythrocyte chromatin was digested with micrococcal nuclease as described above, dialyzed overnight against low salt buffer (1 mM EDTA, 10 mM Tris-HCI, pH 7 .4) and examined by sucrose  ... 
doi:10.1083/jcb.99.1.272 pmid:6736129 pmcid:PMC2275636 fatcat:nvywoevj65ebjcqrnyocb6bpoi

Sequence-specific cleavage of chromatin by staphylococcal nuclease can generate an atypical nuclesome pattern

Urs H. Pauli, Thomas Seebeck, Richard Braun
1982 Nucleic Acids Research  
When probed with staphylococcal nuclease, the ribosomal genes appear to be uniformly packed in nucleosomes, in an arrangement which is indistinguishable from the pattern obtained with bulk chromatin.  ...  These observations clearly show that great caution needs to be exerted whenever data from staphylococcal nuclease digestions are interpreted in terms of chromatin structure.  ...  This work was supported by grants Nr. 3.312-0.78 and 3.075-1.81 of the Swiss National Science Foundation.  ... 
doi:10.1093/nar/10.14.4121 pmid:7122237 pmcid:PMC320787 fatcat:okvusf7linbotn5tbfogd3akz4

A structure of potentially active and inactive genes of chicken erythrocyte chromatin upon decondensation

Alexander N. Kukushkin, Svetlana B. Svetlikova, Valery A. Pospelov
1988 Nucleic Acids Research  
When chromatin has been decondensed by incubation of nuolei in 10 mU Tris-buffer, Dfase II generates a tpical nucleosomal repeat, and the differential nuclease sensitivity of the analyzed genes is not  ...  In a solution of low ionic strength chromatin exists as 10 am nucleosomal fibrils (1, 2), but in the presence of divalent ions (0.1-1 mU) or monovalent ions (50-100 mU) the 10 nm fibrils form the 25-30  ...  Overall nuclease digestibility of chromatin is increased by a factor of two in comparison to the digestion at 3 M Mglg12 lease.  ... 
doi:10.1093/nar/16.17.8555 pmid:3419926 pmcid:PMC338576 fatcat:jogwxdnmebg5bbyepizpq6vqka

Chromatin structure of a hyperactive secretory protein gene (in Balbiani ring 2) of Chironomus

R M Widmer, R Lucchini, M Lezzi, B Meyer, J M Sogo, J E Edström, T Koller
1984 EMBO Journal  
Micrococcal nuclease digestion of purified nuclei followed by DNA transfer and hybridization revealed a smear with no recognizable discrete DNA fragments.  ...  Without pilocarpine stimulation a faint nucleosomal repeat was superimposed upon the smear, and in tissue culture cells a clear nucleosomal repeat was revealed.  ...  This work was supported by Schweizerischer Nationalfonds zur Forderung der wissenschaftlichen Forschung, grants to M. Lezzi and Th.Koller.  ... 
pmid:6086328 pmcid:PMC557570 fatcat:ctlamiokxbcnbnvogp5ftaasem

Alterations in the internucleosomal DNA helical twist in chromatin of human erythroleukemia cells in vivo influences the chromatin higher-order folding

Wladyslaw A. Krajewski
1995 FEBS Letters  
folds in higher-order chromatin structures, as evidenced by spe-4fie alterations in nuclease susceptibility of chromatin.  ...  The results pre-~ented here imply that the alterations in the rotation angle between the adjacent nucleosomes in chromatin of eukaryotic cells in vivo significantly influences the way the chain of nucleosomes  ...  Higher levels of micrococcal nuclease digestion revealed the presence of the typical nucleosome 'ladder' for all the chromatin samples (Fig. 2) , suggesting that these chloroquine concentrations are probably  ... 
doi:10.1016/0014-5793(95)00144-x pmid:7698313 fatcat:rbuipfew5nfm3balgby2lkrcna

Modulation of the higher-order folding of chromatin by deletion of histone H3 and H4 terminal domains

Wladyslaw A. KRAJEWSKI, Juan AUSIÓ
1996 Biochemical Journal  
The ' tails ' of histones H3 and H4 were removed by light in situ trypsin digestion of the nuclei.  ...  The alterations in the higherorder folding of chromatin resulting from this treatment were monitored by ethidium bromide titration.  ...  We would like to acknowledge the Institute of Developmental Biology, Russian Academy of Science, for generosity in providing information on intercalation-mediated chromatin folding (and corresponding methodological  ... 
doi:10.1042/bj3160395 pmid:8687379 pmcid:PMC1217363 fatcat:4d53i2i7yjavjl5wagkejg6hhe

Organization of internucleosomal DNA in rat liver chromatin

F Strauss, A Prunell
1983 EMBO Journal  
A detailed analysis of the length distribution of DNA in nucleosome dimers trimmed with exonuclease III and S1 nuclease suggests that the previously described variation of internucleosomal distance in  ...  Results obtained upon digestion of chromatin with DNase II further suggest that lengths of internucleosomal DNA are integral multiples of the helical repeat of the DNA plus approximately 5 bp.  ...  Under these conditions, DNase II digests chromatin in the 'unclassical', 100 nucleotide, cleavage mode . Digestion in the 'classical' cleavage mode (Horz and  ... 
pmid:11894908 pmcid:PMC555085 fatcat:2r3yia43fba3rirtscjpkivu3u

The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones

Corrado Spadafora, Pierre Oudet, Pierre Chambon
1978 Nucleic Acids Research  
The in vitro-assembled chromatins were visualized by electron microscopy and the size of the DNA fragments generated by digestion with DNase II was determined.  ...  the variability of the DNA repeat length of native chromatins.  ...  The DNA repeat length of a given chromatin can be determined by nuclease digestion and corresponds to the amount of DNA contained in a nucleosome (for references, see [1] [2] [3] .  ... 
doi:10.1093/nar/5.10.3479 pmid:214759 pmcid:PMC342689 fatcat:mxq6dlndyje6vn6ttispu6zfba

Mitotic chromosome scaffold structure: New approaches to an old controversy

A. S. Belmont
2002 Proceedings of the National Academy of Sciences of the United States of America  
Restriction digest of chromatin typically is limited to linker DNA between nucleosomes, leading to an Ϸ10-fold reduction in available sites.  ...  nucleosomes, just two turns of a helical nucleosome folding in a solenoid model of 30-nm chromatin fibers (37) .  ... 
doi:10.1073/pnas.262672799 pmid:12461163 pmcid:PMC138527 fatcat:d4ixeox43zaknc23c3dyjv225q

Nucleosome assembly in mammalian cell extracts before and after DNA replication

C Gruss, C Gutierrez, W C Burhans, M L DePamphilis, T Koller, J M Sogo
1990 EMBO Journal  
This assembly was monitored by topoisomer formation, micrococcal nuclease digestion and psoralen crosslinking of the DNA.  ...  However, the highly sensitive psoralen assay revealed that DNA replication appears to facilitate precise folding of DNA in the nucleosome.  ...  J.M.S. was partially supported by the Roche Research Foundation (Basel, Switzerland).  ... 
pmid:2167837 pmcid:PMC552007 fatcat:r3njhvzshffxbi3sobnxxpveha
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