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Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc

Junguk Ko, Jae-Chern Yoo
2019 Sensors  
This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD.  ...  Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD.  ...  In this study, we proposed a non-contact temperature control system that realizes accurate thermal cycles for an end-point polymerase chain reaction on a lab-on-a-disc (PCR-LOD) to detect Salmonella, which  ... 
doi:10.3390/s19112621 fatcat:m4h6zonjlfb4ro4imszv7kjtfa

Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors

Sara Santiago-Felipe, Luis A. Tortajada-Genaro, Javier Carrascosa, Rosa Puchades, Ángel Maquieira
2016 Biosensors & bioelectronics  
Besides, high temperatures lead to 50 variations in the volume reaction and gas bubble formation, one of the main drawbacks in lab-on-a-chip.  ...  The system can be equipped with different components to monitor and control enclosure temperatures.  ...  The most widespread method for amplification is polymerase chain third layer, used to seal the system. The resulting disc contained micro-reactors for 96 assays.  ... 
doi:10.1016/j.bios.2015.12.045 pmid:26716424 fatcat:umbsmil2vfbohbcgatrbs6ehje

One-pot isothermal DNA amplification – Hybridisation and detection by a disc-based method

Sara Santiago-Felipe, Luis A. Tortajada-Genaro, Sergi Morais, Rosa Puchades, Ángel Maquieira
2014 Sensors and actuators. B, Chemical  
The method principle is based on recombinase polymerase amplification 12 (RPA) and detection in the microarray format by compact disc technology as a high-13 throughput sensing platform.  ...  A 22 triplex assay was satisfactorily compared to the non-integrated protocols. Food and 23 vaccine samples were analysed in a shorter time with less handling.  ...  Higher responses were 319 achieved for the non-contact heating systems (oven and IR lamp), if compared to 320 systems based on heating by contact (F=17.58>F4,5=5.19, p-value<0.05) .  ... 
doi:10.1016/j.snb.2014.07.073 fatcat:ee4wny6tvbdidifvbjtdchvaey

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

Mary Amasia, Seok-Won Kang, Debjyoti Banerjee, Marc Madou
2013 Biomicrofluidics  
platform for polymerase chain reaction (PCR) amplification.  ...  In these miniaturized systems, the heating process is often performed more rapidly, making the temperature control more difficult, and adding complexity to the integrated hardware system.  ...  INTRODUCTION Polymerase chain reaction (PCR) is a common analytical technique used for the detection of biological samples, especially for samples with low target concentrations.  ... 
doi:10.1063/1.4789756 pmid:24403998 pmcid:PMC3574097 fatcat:txarnp34ofbhhdzz7rdjeneune

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

Lena Gorgannezhad, Helen Stratton, Nam-Trung Nguyen
2019 Micromachines  
In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers.  ...  Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications.  ...  Spatial Polymerase Chain Reaction If the sample moves continuously in a microchannel through regions with fixed temperatures, thermal cycling occurs spatially.  ... 
doi:10.3390/mi10060408 pmid:31248141 pmcid:PMC6630468 fatcat:2xcbooo6pnbwhblrp7t7lna7wa

Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

Minghui Ji, Yun Xia, Jacky Fong-Chuen Loo, Lang Li, Ho-Pui Ho, Jianan He, Dayong Gu
2020 RSC Advances  
Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.  ...  throughput. 30 Non-contact sensing and heating for temperature regulation has simplied the embedment of the microuidic disc onto spinning platform to support robust thermocycling without the need of  ...  The initial PCR conditions were established according to the melting temperature (T m ) value of the primer pairs and the probe and the recommended reaction temperature of the DNA polymerases.  ... 
doi:10.1039/d0ra04507a pmid:35519051 pmcid:PMC9056731 fatcat:47i6zklkurfbvfeayfxcbdw2ia

Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform

Saraí M. Torres Delgado, David J. Kinahan, Fralett Suárez Sandoval, Lourdes Albina Nirupa Julius, Niamh A. Kilcawley, Jens Ducrée, Dario Mager
2016 Lab on a Chip  
Wireless power technology for centrifugal microfluidics to bridge the gap between Lab-on-a-Disc and Lab-on-a-Chip by placing the sensors in the frame of reference of the spinning disc.  ...  O′Connell of DCU for providing advice and guidance on the biological aspects of this work.  ...  The authors are also thankful to Prof. Dr. Ulrike Wallrabe for providing access to her laboratories at the University of Freiburg and to Brian Henderson and Dr. Triona M.  ... 
doi:10.1039/c6lc00973e pmid:27713985 fatcat:huadpse6pje73fc3abquxshr7i

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Vicky Troger Katja Niemann
2015 Journal of Nanomedicine & Nanotechnology  
Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results  ...  Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis.  ...  However, some isothermal reactions need a precise temperature control as well.  ... 
doi:10.4172/2157-7439.1000282 fatcat:pufvqwcli5ft3aevutwaimbvzu

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

2017 Micromachines  
This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care  ...  A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point  ...  PMMA layer; (c) fully assembled lab-on-a-disc.  ... 
doi:10.3390/mi8090266 pmid:30400456 pmcid:PMC6190060 fatcat:ocezyjbugba6zouidqmzx3zat4

Integrated LAMP and immunoassay platform for diarrheal disease detection

Christopher R. Phaneuf, Betty Mangadu, Huu M. Tran, Yooli K. Light, Anchal Sinha, Frank W. Charbonier, Tyler P. Eckles, Anup K. Singh, Chung-Yan Koh
2018 Biosensors & bioelectronics  
A disposable disc with automatic aliquoting inlets is paired with a non-contact heating system and precise rotary control system to yield an easy-to-use, field-deployable platform with versatile screening  ...  The full panel of toxins and bacteria were reliably detected simultaneously on a single disc at clinically relevant sample concentrations in less than an hour.  ...  KM, Abd-Allah F, Abera SF, Abreu DMX, Abu-Raddad LJ, Abyu GY, Achoki T, Adelekan AL, Ademi Z, Adou AK, Adsuar JC, Afanvi KA, Afshin A, Agardh EE, Agarwal A, Agrawal A, Kiadaliri AA, Ajala ON, Akanda AS  ... 
doi:10.1016/j.bios.2018.08.005 pmid:30172236 pmcid:PMC6145809 fatcat:nxlmczqrebdtpkyqebadctqthu

Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification

Michael Mauk, Changchun Liu, Jinzhao Song, Haim Bau
2015 Microarrays  
"lab on a chip" NAAT devices.  ...  A portable, miniaturized POC NAAT with performance comparable to conventional PCR (polymerase-chain reaction)-based tests in clinical laboratories can be realized with a disposable, palm-sized, plastic  ...  Mauk contributed to the sections relating to background, literature review, and chips for NA isolation, amplification and detection, Changchun Liu contributed to the sections on plasma extraction and nuclemeter  ... 
doi:10.3390/microarrays4040474 pmid:27600235 pmcid:PMC4996405 fatcat:hr65xhdx25cxpbfqwpakr5wb34

Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products

Luis Antonio Tortajada-Genaro, Alejandro Rodrigo, Elizabeth Hevia, Salvador Mena, Regina Niñoles, Ángel Maquieira
2015 Analytical and Bioanalytical Chemistry  
Regarding to the food safety control of meat products, this approach is able to screen products along the entire production/distribution chain with best performances 380 than methods based on integrated  ...  Discs were incubated during 25 min at room temperature and darkness.  ...  Probes (100 nM) prepared in printing solution (190 nM streptavidin, 50 mM carbonate buffer, pH 9.6 and 1 % glycerol (v/v)), were transferred to the disk (50 nL) with a non-contact arrayer (AD 1500 BioDot  ... 
doi:10.1007/s00216-015-8890-0 pmid:26198111 fatcat:b7kv2jitljezdilqilkn7fq24a

Towards non- and minimally instrumented, microfluidics-based diagnostic devices

Bernhard Weigl, Gonzalo Domingo, Paul LaBarre, Jay Gerlach
2008 Lab on a Chip  
. † Part of a special issue on Point-of-care Microfluidic Diagnostics; Guest Editors -Professor Kricka and Professor Sia.  ...  can be performed in formats similar to that of a strip test.  ...  Acknowledgments The authors acknowledge funding for a portion of this work from the National Institutes of Health (NIAID, as well as funding from NIBIB to establish the Center for Point-of-Care Diagnostics  ... 
doi:10.1039/b811314a pmid:19023463 pmcid:PMC2776042 fatcat:bo7x3jxd6fa55knrvyve36ioe4

Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform

Celina M. Miyazaki, Eadaoin Carthy, David J. Kinahan
2020 Processes  
Lab-on-a-Disc (LoaD) biosensors are increasingly a promising solution for many biosensing applications.  ...  systems.  ...  " or "lab-on-a-CD" or "bio-disk" or "lab-CD" or "lab-disc" (connected red circles).  ... 
doi:10.3390/pr8111360 fatcat:imfjzeso7ffofgfqzqyjd6ofmy

Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)

Luis A. Tortajada-Genaro, Sara Santiago-Felipe, Mary Amasia, Aman Russom, Ángel Maquieira
2015 RSC Advances  
disc drive to read the results.  ...  The proposed device, for massive DNA-based screening in limited-resource settings, comprises a centrifugal platform to perform isothermal solid-phase amplification in microarray format and a digital versatile  ...  The Spanish Ministry of Education and Science provided S.S.F. with a grant for her PhD studies.  ... 
doi:10.1039/c5ra02778k fatcat:jbbqdgz5z5bw3h3a2grvfwuw3u
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