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Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc
2019
Sensors
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This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. ...
Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. ...
In this study, we proposed a non-contact temperature control system that realizes accurate thermal cycles for an end-point polymerase chain reaction on a lab-on-a-disc (PCR-LOD) to detect Salmonella, which ...
doi:10.3390/s19112621
fatcat:m4h6zonjlfb4ro4imszv7kjtfa
Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors
2016
Biosensors & bioelectronics
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Besides, high temperatures lead to 50 variations in the volume reaction and gas bubble formation, one of the main drawbacks in lab-on-a-chip. ...
The system can be equipped with different components to monitor and control enclosure temperatures. ...
The most widespread method for amplification is polymerase chain third layer, used to seal the system. The resulting disc contained micro-reactors for 96 assays. ...
doi:10.1016/j.bios.2015.12.045
pmid:26716424
fatcat:umbsmil2vfbohbcgatrbs6ehje
One-pot isothermal DNA amplification – Hybridisation and detection by a disc-based method
2014
Sensors and actuators. B, Chemical
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The method principle is based on recombinase polymerase amplification 12 (RPA) and detection in the microarray format by compact disc technology as a high-13 throughput sensing platform. ...
A 22 triplex assay was satisfactorily compared to the non-integrated protocols. Food and 23 vaccine samples were analysed in a shorter time with less handling. ...
Higher responses were 319 achieved for the non-contact heating systems (oven and IR lamp), if compared to 320 systems based on heating by contact (F=17.58>F4,5=5.19, p-value<0.05) . ...
doi:10.1016/j.snb.2014.07.073
fatcat:ee4wny6tvbdidifvbjtdchvaey
Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification
2013
Biomicrofluidics
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platform for polymerase chain reaction (PCR) amplification. ...
In these miniaturized systems, the heating process is often performed more rapidly, making the temperature control more difficult, and adding complexity to the integrated hardware system. ...
INTRODUCTION Polymerase chain reaction (PCR) is a common analytical technique used for the detection of biological samples, especially for samples with low target concentrations. ...
doi:10.1063/1.4789756
pmid:24403998
pmcid:PMC3574097
fatcat:txarnp34ofbhhdzz7rdjeneune
Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology
2019
Micromachines
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In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. ...
Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. ...
Spatial Polymerase Chain Reaction If the sample moves continuously in a microchannel through regions with fixed temperatures, thermal cycling occurs spatially. ...
doi:10.3390/mi10060408
pmid:31248141
pmcid:PMC6630468
fatcat:2xcbooo6pnbwhblrp7t7lna7wa
Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform
2020
RSC Advances
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Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection. ...
throughput. 30 Non-contact sensing and heating for temperature regulation has simplied the embedment of the microuidic disc onto spinning platform to support robust thermocycling without the need of ...
The initial PCR conditions were established according to the melting temperature (T m ) value of the primer pairs and the probe and the recommended reaction temperature of the DNA polymerases. ...
doi:10.1039/d0ra04507a
pmid:35519051
pmcid:PMC9056731
fatcat:47i6zklkurfbvfeayfxcbdw2ia
Fully automated chemiluminescence detection using an electrified-Lab-on-a-Disc (eLoaD) platform
2016
Lab on a Chip
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Wireless power technology for centrifugal microfluidics to bridge the gap between Lab-on-a-Disc and Lab-on-a-Chip by placing the sensors in the frame of reference of the spinning disc. ...
O′Connell of DCU for providing advice and guidance on the biological aspects of this work. ...
The authors are also thankful to Prof. Dr. Ulrike Wallrabe for providing access to her laboratories at the University of Freiburg and to Brian Henderson and Dr. Triona M. ...
doi:10.1039/c6lc00973e
pmid:27713985
fatcat:huadpse6pje73fc3abquxshr7i
Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems
2015
Journal of Nanomedicine & Nanotechnology
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Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results ...
Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis. ...
However, some isothermal reactions need a precise temperature control as well. ...
doi:10.4172/2157-7439.1000282
fatcat:pufvqwcli5ft3aevutwaimbvzu
Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies
2017
Micromachines
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This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care ...
A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point ...
PMMA layer; (c) fully assembled lab-on-a-disc. ...
doi:10.3390/mi8090266
pmid:30400456
pmcid:PMC6190060
fatcat:ocezyjbugba6zouidqmzx3zat4
Integrated LAMP and immunoassay platform for diarrheal disease detection
2018
Biosensors & bioelectronics
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A disposable disc with automatic aliquoting inlets is paired with a non-contact heating system and precise rotary control system to yield an easy-to-use, field-deployable platform with versatile screening ...
The full panel of toxins and bacteria were reliably detected simultaneously on a single disc at clinically relevant sample concentrations in less than an hour. ...
KM, Abd-Allah F, Abera SF, Abreu DMX, Abu-Raddad LJ, Abyu GY, Achoki T, Adelekan AL, Ademi Z, Adou AK, Adsuar JC, Afanvi KA, Afshin A, Agardh EE, Agarwal A, Agrawal A, Kiadaliri AA, Ajala ON, Akanda AS ...
doi:10.1016/j.bios.2018.08.005
pmid:30172236
pmcid:PMC6145809
fatcat:nxlmczqrebdtpkyqebadctqthu
Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification
2015
Microarrays
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"lab on a chip" NAAT devices. ...
A portable, miniaturized POC NAAT with performance comparable to conventional PCR (polymerase-chain reaction)-based tests in clinical laboratories can be realized with a disposable, palm-sized, plastic ...
Mauk contributed to the sections relating to background, literature review, and chips for NA isolation, amplification and detection, Changchun Liu contributed to the sections on plasma extraction and nuclemeter ...
doi:10.3390/microarrays4040474
pmid:27600235
pmcid:PMC4996405
fatcat:hr65xhdx25cxpbfqwpakr5wb34
Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products
2015
Analytical and Bioanalytical Chemistry
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Regarding to the food safety control of meat products, this approach is able to screen products along the entire production/distribution chain with best performances 380 than methods based on integrated ...
Discs were incubated during 25 min at room temperature and darkness. ...
Probes (100 nM) prepared in printing solution (190 nM streptavidin, 50 mM carbonate buffer, pH 9.6 and 1 % glycerol (v/v)), were transferred to the disk (50 nL) with a non-contact arrayer (AD 1500 BioDot ...
doi:10.1007/s00216-015-8890-0
pmid:26198111
fatcat:b7kv2jitljezdilqilkn7fq24a
Towards non- and minimally instrumented, microfluidics-based diagnostic devices
2008
Lab on a Chip
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. † Part of a special issue on Point-of-care Microfluidic Diagnostics; Guest Editors -Professor Kricka and Professor Sia. ...
can be performed in formats similar to that of a strip test. ...
Acknowledgments The authors acknowledge funding for a portion of this work from the National Institutes of Health (NIAID, as well as funding from NIBIB to establish the Center for Point-of-Care Diagnostics ...
doi:10.1039/b811314a
pmid:19023463
pmcid:PMC2776042
fatcat:bo7x3jxd6fa55knrvyve36ioe4
Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform
2020
Processes
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Lab-on-a-Disc (LoaD) biosensors are increasingly a promising solution for many biosensing applications. ...
systems. ...
" or "lab-on-a-CD" or "bio-disk" or "lab-CD" or "lab-disc" (connected red circles). ...
doi:10.3390/pr8111360
fatcat:imfjzeso7ffofgfqzqyjd6ofmy
Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)
2015
RSC Advances
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disc drive to read the results. ...
The proposed device, for massive DNA-based screening in limited-resource settings, comprises a centrifugal platform to perform isothermal solid-phase amplification in microarray format and a digital versatile ...
The Spanish Ministry of Education and Science provided S.S.F. with a grant for her PhD studies. ...
doi:10.1039/c5ra02778k
fatcat:jbbqdgz5z5bw3h3a2grvfwuw3u
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