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Clinicopathological Study of Superficial Depressed Type (Type IIc) of Early Colorectal Cancers

Yuji INOUE, Shigeru SUZUKI, Mamoru SUZUKI, Bunei IIZUKA, Toru NAKAMURA, Naoko HONMA, Hiroyuki ITO, Masatugu HUKUSHIMA, Naoko TURUMI, Kounen IIMURA, Satiko SHINOZAKI, Ken TAKASAKI
1999 Gastroenterological Endoscopy  
doi:10.11280/gee1973b.41.1075 fatcat:pcuqniyvereptbihrjudhuvmma

Unmyelinated Nerve Fibre Analysis of the Human Lesser Splanchnic Nerve

Kazumasa SUZUKI, Naoki SHIRAISHI, Noboru GOTO, Masataka SUZUKI, Naoko NONAKA
2000 Okajimas Folia Anatomica Japonica  
The aim of the present study is to analyse unmyelinated nerve fibres of the human lesser splanchnic nerve in relation to the ageing process. With the help of an image-analyser, we examined 30 human lesser splanchnic nerves. The analysis was conducted with the use of a new staining method that makes it possible to discriminate various structures of the nervous tissue. Our report provides for the first time information on the ageing process of the human lesser splanchnic nerve fibres. The results
more » ... indicate that a decrease in transverse area and perimeter of unmyelinated axons is one of the important changes occurring in the human lesser splanchnic nerve during the ageing process.
doi:10.2535/ofaj1936.76.6_285 pmid:10774226 fatcat:eusov2a6dzabzifjfudd7sl3la

Zinc Transporter in Plants

Suzuki, T. Tsukamoto, M. Takahashi, H. Nakanishi, S. Mori & N.K. Nishizawa: Plant J., in press. 15) S.A. Ramesh, R. Shin, D.J. Eide & D.P.  ...  Suzuki, T. Kobayashi, M. Takahashi, H. Nakanishi, S. Mori & N.K. Nishizawa: J. Exp. Bot., in press. 17) S. Yamashita, C. Miyagi, T. Fukuda, N. Kagara, Y.-S. Che & T.  ... 
doi:10.1271/kagakutoseibutsu1962.44.233 fatcat:j3qbd5sfovc4tnxlqtqxj7vize


Atsuhisa TAMURA, Naoko HIGAKI, Kei KUSAKA, Shunsuke AKASHI, Junichi SUZUKI, Masahiro SHIMADA, Jun SUZUKI, Masahiro KAWASHIMA, Junko SUZUKI, Nobuhiro OSHIMA, Kimihiko MASUDA, Hirotoshi MATSUI (+8 others)
2013 Kekkaku (Tuberculosis)  
The aim of this study was to investigate the current status of doctor s delay in diagnosing endobronchial tuberculosis (EBTB) and to elucidate the risk factors contributing to the delay. [Methods] Retrospective clinicopathological analysis. [Patients] Sixty-two patients with EBTB were admitted at our hospital between 1999 and 2010. Their backgrounds, symptoms, diagnoses at initial consultation, delay in diagnosis, and clinical examination results were analyzed. [Results] Of the 62 patients, 59
more » ... ad acid-fast, bacillipositive sputum smear test results at admission. Among the 40 patients with total diagnostic delay of more than 2 months, only 11 experienced long patient s delay exceeding 2 months. However, 22 patients experienced long doctor s delay of more than 2 months (28% vs. 55%, respectively, p<0.05), suggesting that doctor s delay contributes more to total delay than patient s delay. Fever was less frequent in patients with long doctor s delays than in those without (0% vs. 18%, respectively), at the initial consultation. In addition, radiographs showed that patients with long doctor s delays more frequently presented with shadows in the lower lung fi eld (50% vs. 23%, p<0.05), and most of these patients had noncavitary shadows on admission. All 7 patients diagnosed with bronchial asthma at the initial consultation had long doctor s delays. [Conclusion] These fi ndings demonstrate that long doctor s delays in diagnosing EBTB remain an issue. The clinical features of EBTB with long doctor s delays were confi rmed to be quite different from those of pulmonary tuberculosis.
doi:10.11400/kekkaku.88.9 fatcat:m2rhlthhofd2ncljife5nz7uv4

Bioluminescent coelenterazine derivatives with imidazopyrazinone C-6 extended substitution

Ryo Nishihara, Hideyuki Suzuki, Emi Hoshino, Sakura Suganuma, Moritoshi Sato, Tsuyoshi Saitoh, Shigeru Nishiyama, Naoko Iwasawa, Daniel Citterio, Koji Suzuki
2015 Chemical Communications  
Coelenterazine derivatives with extended substituents show significant bioluminescence emission with known renilla luciferase variants, indicating a promising method to develop substrates with superior optical properties.
doi:10.1039/c4cc06886f pmid:25407088 fatcat:6olmjeezlbe4pf5p3tglzpqieu

Loss of ribosomal RNA modification causes developmental defects in zebrafish

Sayomi Higa-Nakamine, Takeo Suzuki, Tamayo Uechi, Anirban Chakraborty, Yukari Nakajima, Mikako Nakamura, Naoko Hirano, Tsutomu Suzuki, Naoya Kenmochi
2011 Nucleic Acids Research  
Non-coding RNAs (ncRNAs) play key roles in diverse cellular activities, and efficient ncRNA function requires extensive posttranscriptional nucleotide modifications. Small nucleolar RNAs (snoRNAs) are a group of ncRNAs that guide the modification of specific nucleotides in ribosomal RNAs (rRNAs) and small nuclear RNAs. To investigate the physiological relevance of rRNA modification in vertebrates, we suppressed the expression of three snoRNAs (U26, U44 and U78), either by disrupting the host
more » ... e splicing or by inhibiting the snoRNA precursor processing, and analyzed the consequences of snoRNA loss-of-function in zebrafish. Using a highly sensitive mass spectrometric analysis, we found that decreased snoRNA expression reduces the snoRNA-guided methylation of the target nucleotides. Impaired rRNA modification, even at a single site, led to severe morphological defects and embryonic lethality in zebrafish, which suggests that rRNA modifications play an essential role in vertebrate development. This study highlights the importance of posttranscriptional modifications and their role in ncRNA function in higher eukaryotes.
doi:10.1093/nar/gkr700 pmid:21908402 pmcid:PMC3245925 fatcat:vniwxld5tvfqjoeb5rk6sphmqa

Accumulation of starch in Zn-deficient rice

Motofumi Suzuki, Khurram Bashir, Haruhiko Inoue, Michiko Takahashi, Hiromi Nakanishi, Naoko K Nishizawa
2012 Rice  
Similarly the secretion of MAs increases in Zn deficient barley, while it decreases in rice (Suzuki et al. 2006; Suzuki et al. 2008) .  ...  Zn-DMA, is suggested to be preferred over Zn 2+ for uptake in barley roots (Suzuki et al. 2006) , while rice roots absorb less Zn-DMA compared to Zn 2+ (Suzuki et al. 2008) .  ... 
doi:10.1186/1939-8433-5-9 pmid:27234235 pmcid:PMC5520845 fatcat:xrel2g2eabffzmakcnjfpwwdvu

Bound Substrate in the Structure of Cyanobacterial Branching Enzyme Supports a New Mechanistic Model

Mari Hayashi, Ryuichiro Suzuki, Christophe Colleoni, Steven G. Ball, Naoko Fujita, Eiji Suzuki
2017 Journal of Biological Chemistry  
Edited by Norma Allewell Branching enzyme (BE) catalyzes the formation of ␣-1,6-glucosidic linkages in amylopectin and glycogen. The reaction products are variable, depending on the organism sources, and the mechanistic basis for these different outcomes is unclear. Although most cyanobacteria have only one BE isoform belonging to glycoside hydrolase family 13, Cyanothece sp. ATCC 51142 has three isoforms (BE1, BE2, and BE3) with distinct enzymatic properties, suggesting that investigations of
more » ... hese enzymes might provide unique insights into this system. Here, we report the crystal structure of ligand-free wild-type BE1 (residues 5-759 of 1-773) at 1. Å resolution. The enzyme consists of four domains, including domain N, carbohydrate-binding module family 48 (CBM48), domain A containing the catalytic site, and domain C. The central domain A displays a (␤/␣) 8barrel fold, whereas the other domains adopt ␤-sandwich folds. Domain N was found in a new location at the back of the protein, forming hydrogen bonds and hydrophobic interactions with CBM48 and domain A. Site-directed mutational analysis identified a mutant (W610N) that bound maltoheptaose with sufficient affinity to enable structure determination at 2.30 Å resolution. In this structure, maltoheptaose was bound in the active site cleft, allowing us to assign subsites ؊7 to ؊1. Moreover, seven oligosaccharide-binding sites were identified on the protein surface, and we postulated that two of these in domain A served as the entrance and exit of the donor/acceptor glucan chains, respectively. Based on these structures, we propose a substrate binding model explaining the mechanism of glycosylation/deglycosylation reactions catalyzed by BE.
doi:10.1074/jbc.m116.755629 pmid:28193843 pmcid:PMC5392689 fatcat:usinqx6wtjdl3futp2dzxfkwim

Crystallization and crystallographic analysis of branching enzymes fromCyanothecesp. ATCC 51142

Mari Hayashi, Ryuichiro Suzuki, Christophe Colleoni, Steven G. Ball, Naoko Fujita, Eiji Suzuki
2015 Acta Crystallographica Section F Structural Biology Communications  
Several cyanobacterial species, includingCyanothecesp. ATCC 51142, remarkably have four isoforms of α-glucan branching enzymes (BEs). Based on their primary structures, they are classified into glycoside hydrolase (GH) family 13 (BE1, BE2 and BE3) or family 57 (GH57 BE). In the present study, GH13-type BEs fromCyanothecesp. ATCC 51142 (BE1, BE2 and BE3) have been overexpressed inEscherichia coliand biochemically characterized. The recombinant BE1 was crystallized by the hanging-drop
more » ... sion method. Crystals of BE1 were obtained at 293 K in the presence of 0.2 MMg2+, 7–10%(w/v) ethanol, 0.1 MHEPES–NaOH pH 7.2–7.9. The crystals belonged to the tetragonal space groupP41212, with unit-cell parametersa = b = 133.75,c= 185.90 Å, and diffracted to beyond 1.85 Å resolution. Matthews coefficient calculations suggested that the crystals of BE1 contained two molecules in the asymmetric unit.
doi:10.1107/s2053230x1501198x pmid:26249708 pmcid:PMC4528950 fatcat:5z3y3d4is5codfygv6x25smyia


2009 Fukushima Journal of Medical Science  
It is well known that unilateral profound sensorineural hearing loss is caused by mumps; however, bilateral deafness is rare. Herein we report a case of bilateral profound hearing loss caused by mumps infection in a four-year-old boy. Labyrinthitis due to the mumps virus was suspected. His verbal understanding was poor, and he completely stopped talking. He was soon fitted with a hearing aid, but it proved insufficient. Thereupon, cochlear implantation was performed on his left ear. Six months
more » ... fter the operation, his speech perception and speech production were improved. In cases of bilateral profound hearing loss due to mumps infection conservative therapy is ineffective; therefore, cochlear implantation is recommended. Vaccine coverage for mumps virus is also strongly recommended in Japan.
doi:10.5387/fms.55.32 fatcat:c2kzbkvisbct7jt5bwrxckgxe4

Formulation Study of Intravesical Oxybutynin Instillation Solution with Enhanced Retention in Bladder

Takehisa Hanawa, Chikako Tsuchiya, Naoko Endo, Kazumi Hanawa, Masahiko Suzuki, Taeko Suzuki, Kiminori Mohri, Toshio Oguchi
2008 Chemical and pharmaceutical bulletin  
A formulation study of intravesical oxybutynin (OB) preparations was carried out in order to improve the effectiveness in intravesical instillation therapy for spastic neurogenetic bladder. Sodium hyaluronate (HYA) was introduced to enhance the muco-adhesiveness of the instillation preparation, and the physicochemical properties of the OB formulation were evaluated in comparison with a conventional formulation containing hydroxypropylcellulose (HPC). The viscous properties and in vitro
more » ... ess increased with the amount of the polymeric additives, and retention properties of OB in rabbit bladder were comparable after addition of 0.4% HYA and 1.0% HPC. HYA was able to enhance the intravesical retention properties of OB instillation solution to a lesser degree than HPC, it seemed to be a useful additive in the OB instillation due to its safety and mucosal-protective effect.
doi:10.1248/cpb.56.1073 pmid:18670105 fatcat:wuhbj4hwnbfqjn76fkvruwbvry

Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy

Junichi Yasutake, Yusuke Suzuki, Hitoshi Suzuki, Naoko Hiura, Hiroyuki Yanagawa, Yuko Makita, Etsuji Kaneko, Yasuhiko Tomino
2015 Nephrology, Dialysis and Transplantation  
doi:10.1093/ndt/gfv221 pmid:26109484 pmcid:PMC4513896 fatcat:6u3otzrnhbhjxitiwzo67qnwpy


1988 JSM Mycotoxins  
doi:10.2520/myco1975.1988.1supplement_113 fatcat:ryzrmiry3jgxhatepg572yapt4

MPAI (Mass Probes Aided Ionization) Method for Total Analysis of Biomolecules by Mass Spectrometry

Aki HONDA, Shinichiro HAYASHI, Hiroki HIFUMI, Yuya HONMA, Noriyuki TANJI, Naoko IWASAWA, Yoshio SUZUKI, Koji SUZUKI
2007 Analytical Sciences  
doi:10.2116/analsci.23.11 pmid:17213616 fatcat:33tkqkp4erhmzhbkrk5uubmu5y

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots

Kazuya Suzuki, Reiko Itai, Koichiro Suzuki, Hiromi Nakanishi, Naoko-Kishi Nishizawa, Etsuro Yoshimura, Satoshi Mori
1998 Plant Physiology  
Comparison of 2D profiles of proteins in barley (Hordeum vulgare L.) roots under Fesufficient and Fe-deficient conditions (Suzuki et al., 1995 (Suzuki et al., , 1997 allowed us to identify a 36-kD protein  ...  Protein spots C, D, G, and V had previously been observed to increase during Fe deficiency (Mori et al., 1988; Suzuki et al., 1995) , but the W, X, and Y spots were newly identified in this study.  ... 
doi:10.1104/pp.116.2.725 pmid:9489019 pmcid:PMC35132 fatcat:u564diibn5cplmnx2spvvjkpzy
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