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Biochemistry & Analytical Biochemistry
Cervical carcinoma is one of the highest causes of mortality in female and the number of patients is gradually increasing in the world. HeLa is a cervical carcinoma cell line and first established human immortalized cell line from cervical cancer patient. On the other hands, Heat shock transcription factor 1 (HSF1) is highly expressed in several cancer cells and tumors, and also involved in the malignancy. In order to investigate the role of HSF1 in cervical carcinoma, I established HeLa celldoi:10.15761/brcp.1000124 fatcat:jjrng5s22zcqpdvnlgsxgbpicm
more »... nes inducibly expressing constitutively active HSF1 (caHSF1) or dominant negative mutant RgHSF1, in a manner controllable by tetracycline. Expressed caHSF1 significantly suppressed HeLa cell growth, whereas RgHSF1 did not show appreciable effects. G1 arrest occurred in the HeLa cells expressing caHSF1, and cyclinedependent kinase (CDK) inhibitors p16 and p21 were up-regulated. To our knowledge, this is the first report that HSF1 may have a role to suppress the cancer cell growth. But importantly, this study suggests HSF1 may have more complicated roles than expected.
To maintain proteostasis under heat shock conditions, bacterial cells Transcription factor cooperativity with heat shock factor 1 Naoki Hayashida, Mitsuaki Fujimoto and Akira Nakai* Department of Biochemistry ...doi:10.4161/trns.2.2.14962 pmid:21468236 pmcid:PMC3062401 fatcat:ztp5kbttyvgozeo6bsnnjfwbj4
The discoveries by Hayashida and Vakoc's groups may support and confirm the existence of the gene bookmarking function of HSF2. ... Hayashida and his colleagues previously reported that the expression of most HSPs, including HSP27, increases in the same caHSF1-expressing HeLa cells  . ...doi:10.3390/cells11020203 pmid:35053319 pmcid:PMC8773920 fatcat:rndd7lz7vzewfcs5wlbsv6r7s4
KargerAG, Basel 0028-2766/96/0722-0351S8.00/0 ■ Nephron 1996;72:351-352 Sakemi/Hayashida/Ikeda/Baba/Nishihara/ Kohda ...doi:10.1159/000188882 pmid:8684567 fatcat:c4jsp2u5drb7rkdqniswtxs3ae
Nozomi Hayashida is with Health Information Management Section, Kumamoto Rehabilitation Hospital, Japan (e-mail: n-hayashida@krh. marutakai.or.jp). ... provide phone: +81-96-242-6069, fax: +81-96-242-6106, e-mail: email@example.com). ...doi:10.5281/zenodo.1061360 fatcat:ztwzr6wu7fgfhcv2qo6ctvnumi
Mammalian tissues are always exposed to diverse threats from pathological conditions and aging. Therefore, the molecular systems that protect the cells from these threats are indispensable for cell survival. A variety of diseases, including neurodegenerative diseases, cause intracellular damage and disturb homeostasis. Heat shock transcription factor 1 (HSF1) positively regulates heat shock protein (Hsp) and maintains the precise folding of proteins. Moreover, HSF1 induces the non-Hsp genesdoi:10.1538/expanim.15-0023 pmid:26251235 pmcid:PMC4637377 fatcat:6kcqedytgjbmriqkuq2lbjhtw4
more »... ession, and degrades damaged/misfolded protein. Recently, my colleagues and I revealed non-Hsp genes have more protective roles than Hsps at the cellular level. However, whether these protective systems are similarly important to cellular defense in each tissue is still elusive. In this study, I compared polyglutamine (polyQ) protein aggregations/inclusion development in each tissue of WT-and HSF1KO-Huntington's disease (HD) mice, and examined the expression of the eight non-Hsp HSF1 target genes that have a strong suppressive effect on polyQ protein aggregation. Of these genes, Nfatc2, Pdzk3, Cryab, Csrp2, and Prame were detected in most tissues, but the other genes were not. Surprisingly, the obvious effect of HSF1 deficiency on the expression of these five genes was detected in only heart, spleen, and stomach. In addition, polyQ protein aggregations/ inclusion was not detected in any tissues of WT-HD and HSF1KO-HD mice, but higher level of preaggregative polyQ protein was detected in HSF1KO-HD tissues. These results indicate non-Hsp genes are indispensable for the maintenance of intracellular homeostasis in mammalian tissues, resulting in whole body homeostasis.
Upper gastrointestinal (GI) symptoms such as nausea and vomiting are common adverse events associated with selective serotonin reuptake inhibitors (SSRIs), and may result in discontinuation of drug therapy in patients with depressive disorder. Rikkunshi-to (formulation TJ-43), a traditional herbal medicine, has been reported to improve upper GI symptoms and comorbid depressive symptoms in patients with functional dyspepsia. The aim of the present study was to determine if TJ-43 reduces GIdoi:10.1186/1751-0759-1-21 pmid:18001480 pmcid:PMC2204024 fatcat:6h6qh5ov6rfa5gw7kwhf542bja
more »... ms and potentiates an antidepressant effect in a randomized controlled study of depressed patients treated with fluvoxamine (FLV). Methods: Fifty patients with depressive disorder (19-78 years, mean age 40.2 years) were treated with FLV (n=25) or FLV in combination with TJ-43 (FLV+TJ-43) (n=25) for eight weeks. The following parameters of the two groups were compared: The number of patients who complained of adverse events and their symptoms; GI symptoms quality of life (QOL) score, assessed by the Gastrointestinal Symptom Rating Scale (GSRS), Japanese edition, before and two weeks after beginning treatment; and depressive symptoms assessed by the Self-Rating Depression Scale (SDS), before and 2, 4, and 8 weeks after beginning treatment. Results: The number of patients who complained of adverse events in the FLV+TJ-43 group (n=6) was significantly lower than the number complaining in the FLV group (n=13) (P < 0.05). The number of patients who complained of nausea was also lower in the FLV+TJ-43 group (n=3) than in the FLV group (n=9) (P < 0.05). By two weeks after treatment, GSRS scores had improved in -3the FLV+TJ-43 group, but not in the FLV group. SDS scores were not different between the two groups at any of the assessment points. Conclusions: This study suggests that Rikkunshi-to reduces FLV-induced adverse events, especially nausea, and improves QOL related to GI symptoms without affecting the antidepressant effect of FLV. -4 -
The febrile response is elicited by pyrogenic cytokines including IL-6 in response to microorganism infections and diseases in vertebrates. Mammalian HSF1, which senses elevations in temperature, negatively regulates the response by suppressing pyrogenic cytokine expression. We here showed that HSF3, an avian ortholog of mammalian HSF1, directly binds to and activates IL-6 during heat shock in chicken cells. Other components of the febrile response mechanism, such as IL-1b and ATF3, were alsodoi:10.1016/j.febslet.2013.09.012 pmid:24055475 fatcat:oncxyykxujce7ejfrrhbsgnzue
more »... fferently regulated in mammalian and chicken cells. These results suggest that the febrile response is exacerbated by a feed-forward circuit composed of the HSF3-IL-6 pathway in birds.
Yamaguchi Medical Journal
Biol Reprod 2004；70：18-24. ５)Hayashida N, Inouye S, Fujimoto M, Tanaka Y, Izu H, Takaki E, Ichikawa H, Rho J, Nakai A. A novel HSF1-mediated death pathway that is suppressed by heat shock proteins. ...doi:10.2342/ymj.56.111 fatcat:yq4wyzmuurh2lifhkbvb6mls5a
Journal of the Agricultural Engineering Society, Japan
講 座 農 業 集 落 排 水(そ の2) -農 業 集 落 排 水 の 計 画- 林 田 直 樹 † (Naoki HAYASHIDA) 1.は じ め に 農業集 落 排 水 事 業 は,従 来 の土 地 改 良事 業 と は多 くの点 で異 な って い る。 ...doi:10.11408/jjsidre1965.61.6_549 fatcat:ai2lnbmt5nh5xcds6pqvsnyhoe
The Japanese Journal of Physiology
HAYASHIDA, K. OKUI, and Y. FUKUDA ...doi:10.2170/jjphysiol.36.783 fatcat:lezx47wzxrd5hgeuskr734gwhq
An obese 79-year-old diabetic woman was admitted with a history of intermittent abdominal pain and vomitting. Two years previously, she had been investigated in our hospital for cholecystolithiasis. Plain radiographs of abdomen with the patient supine revelaed air in the biliary tree and incomplete small-bowel obstruction. With the pre-operative diagnosis of gallstone ileus, laparotomy was performed, and 2.5cm calculus was removed from the jejunum. A large cholecysto-duodenal fistula was noted,doi:10.3919/ringe1963.46.521 fatcat:afx4vbeerrgg3fviysud4pgetm
more »... but no further surgery was performed on account of high risk. Recovery was satisfactory and free from complications. We analyzed 160 cases of gallstone ileus in Japan with special reference to treatment. We classified gallstone ileus into four types, according to the number, position of the stones before they enter the bowels. In type 1, there was no difference in the incidence of complications between the ileus operation group and the ileus operation plus radical operation group. In types 2 and 3, the incidence of complications was significantly lower in the ileus operation plus radical operation group than in the ileus operaton group. These results suggested that surgical removal of gallstones that obstruct the intestine will have good results in type 1 patients, but that additional radical surgery should be un dertaken primarily or secondarily in type 2 or 3 patients with gallstone ileus.
Journal of the Agricultural Engineering Society, Japan
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