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ECM remodeling and spatial cell cycle coordination determine tissue growth kinetics [article]

Anna P Ainslie, John Robert Davis, John J Williamson, Ana Ferreira, Alejandro Torres-Sanchez, Andreas Hoppe, Federica Mangione, Matthew B Smith, Enrique Martin-Blanco, Guillaume Salbreux, Nicolas Tapon
2020 bioRxiv   pre-print
Thus, changes in the tissue microenvironment, and a rapid exit from proliferation, control the formation of the adult Drosophila abdomen.  ...  Analysis of the temporal dynamics of tissue growth in the Drosophila abdomen reveals that cell cycle times are spatially correlated and that growth termination occurs through the rapid emergence of a population  ...  Bellaïche, D. Bohmann, B. Stramer, K. Irvine and the Bloomington Drosophila Stock Centre for fly stocks. We are grateful to M.  ... 
doi:10.1101/2020.11.10.376129 fatcat:fciqli42knc2tew7s5gg5fgw6i

A fluorogenic nanobody array tag for prolonged single molecule imaging in live cells [article]

Rajarshi Ghosh, Will Draper, J. Matthew Franklin, Quanming Shi, Jan Liphardt
2017 bioRxiv   pre-print
Prolonged single molecule imaging in live cells requires labels that do not aggregate, have high contrast, and are photo-stable.  ...  The photo-stability of ArrayG and consistently low background allowed us to continuously track single integrins for as long as 105 seconds (2100 frames).  ...  (a) Top: Representative time lapse images of spot bleach and recovery for ArrayG and ArrayD. ~ 10 sec time spacing between images.  ... 
doi:10.1101/111690 fatcat:is2gmleambe7javetorc75swya

Radial Migration Dynamics Is Modulated in a Laminar and Area-Specific Manner During Primate Corticogenesis

Veronique Cortay, Delphine Delaunay, Dorothée Patti, Elodie Gautier, Nathalie Doerflinger, Pascale Giroud, Kenneth Knoblauch, Cyril Huissoud, Henry Kennedy, Colette Dehay
2020 Frontiers in Cell and Developmental Biology  
The orderly radial migration of cortical neurons from their birthplace in the germinal zones to their final destination in the cortical plate is a prerequisite for the functional assembly of microcircuits  ...  Rodent and primate corticogenesis differ both quantitatively and qualitatively, particularly with respect to the generation of neurons of the supragranular layers.  ...  We also thank Marion Betizeau for help with analysis of Figure 1G and Sabina Pfister and Michael Pfeiffer for developing an in-house software to extract migration kinetics parameters.  ... 
doi:10.3389/fcell.2020.588814 pmid:33178700 pmcid:PMC7596244 fatcat:wz55eppe6bcbnpmhzw2bjult4i

Computer-Assisted Analysis of Biomedical Images [article]

Leonardo Rundo
2021 arXiv   pre-print
Therefore, the computational analysis of medical and biological images plays a key role in radiology and laboratory applications.  ...  Nowadays, the amount of heterogeneous biomedical data is increasing more and more thanks to novel sensing techniques and high-throughput technologies.  ...  High-content time-lapse imaging monitors the responses in living cells over a specific period of time exploiting the activity of fluorescently labeled and unlabeled cells.  ... 
arXiv:2106.04381v1 fatcat:osqiyd3sbja3zgrby7bf4eljfm

Collective motion of cells: from experiments to models

Előd Méhes, Tamás Vicsek
2014 Integrative Biology  
Swarming or collective motion of living entities is one of the most common and spectacular manifestations of living systems that have been extensively studied in recent years.  ...  Acknowledgements We acknowledge support from the EU FP7 ERC COLLMOT GRANT No: 227878. We thank A. Czirok (Eötvös University, Dept. Biological Physics) for useful consultations.  ...  Fig. 7 7 Overview of a time-lapse movie showing the lateral line primordium undergoing a ''U-turn'' maneuver.  ... 
doi:10.1039/c4ib00115j pmid:25056221 fatcat:rbo3ao7bkvadrbv4yuh7rodela

A design methodology for 2D sparse NDE arrays using an efficient implementation of refracted-ray TFM

Jerzy Dziewierz, Timothy Lardner, Anthony Gachagan
2013 2013 IEEE International Ultrasonics Symposium (IUS)  
Thus, ultrasound technologies can be used to enhance the level of complexity of extracellular matrix microenvironments and cellular functions achievable within three-dimensional engineered tissues.  ...  The development of more complex tissues has been limited by the need for techniques that can rapidly organize cells and proteins within large three-dimensional scaffolds, and the need to create vascular  ...  The authors acknowledge the ANR and DGA for their support under the project Metactif, grant ANR-11-ASTR-015.  ... 
doi:10.1109/ultsym.2013.0035 fatcat:th5znfh7y5bklfdbmhb4u6iq2i

Consensus guidelines for the use and interpretation of angiogenesis assays

Patrycja Nowak-Sliwinska, Kari Alitalo, Elizabeth Allen, Andrey Anisimov, Alfred C. Aplin, Robert Auerbach, Hellmut G. Augustin, David O. Bates, Judy R. van Beijnum, R. Hugh F. Bender, Gabriele Bergers, Andreas Bikfalvi (+74 others)
2018 Angiogenesis  
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions  ...  This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation  ...  This work was supported by funding from: Dutch Cancer Society (VU2012-5480 to JRvB and AWG; VU2014-7234 to AWG and PNS); European  ... 
doi:10.1007/s10456-018-9613-x pmid:29766399 fatcat:y6lwcu7745do3nu3ifqjgocxzm

第57回年会予稿集

2019 Seibutsu Butsuri  
We describe the development of biofilms in a 2D microfluidic chamber that enables long-term time-lapse microscopy, single-cell tracking, and control over the microenvironment enabling the analysis of the  ...  This project is an important milestone in the development of artificial cells because it demonstrated proliferation of cells inside vesicles. 1SCA-8 計算折り紙による 3 次元細胞立体構造 3D Cell Structure Optimized by Computational  ...  We report time-course change of the morphological characteristics of circulating tumor cell (CTC) clusters in prostate cancerimplanted rat blood by using an on-chip multi-imaging flow cytometer. 50 μL  ... 
doi:10.2142/biophys.59.s1 fatcat:rsudhlmaanetrhmdxjgsiyorme

2017 ASCB Annual Meeting Abstracts

David G. Drubin
2017 Molecular Biology of the Cell  
This allowed us to track these organelles through time and analyze their inter-organelle contacts, providing a systems-level map of the organelle interactome and how it is perturbed under different physiological  ...  multi-vesicular bodies, MVBs.  ...  Cell 161, 581-594. [8] Köster, D. V., and Mayor, S. (2016). Curr.  ... 
doi:10.1091/mbc.e17-10-0618 pmid:29237772 pmcid:PMC5739290 fatcat:3msjrn3omzcybfanlagykayauy

Symposia

2016 The FEBS Journal  
We study this using the concept of Pareto optimality from engineering and economics.  ...  Jadhav 3 , D. Dorjsuren 3 , D. Maloney 3 , A. Simeonov 3 , P. Jaruga 1 , E. Coskun 1 , A. K. McCullough 2 , R. S.  ...  Results: The median levels of serum MDA, SOD, GSH-Px, IL-1b, AST, LDH, CK, hs-TnT and NT-proBNP and significant difference from the control or sham groups.  ... 
doi:10.1111/febs.13805 fatcat:5bh6wnb75nbijopqvhnzhv3bmi

13th EBSA congress, July 24–28, 2021, Vienna, Austria

2021 European Biophysics Journal  
Welcome from the President of EBSA The world is dealing with one of its greatest challenges, and thanks to the amazing contribution of science and the development, in record time, of vaccines against SARS-CoV  ...  EBSA is really grateful to our host colleagues from the Austrian Biophysical Society for accepting the challenge of organizing our Congress in Vienna during these extremely difficult times, despite being  ...  DNA self-assembled monolayers are studied by a multi-technique approach to optimize the sensing platform, by tuning ssDNA immobilization time, concentration of ssDNA and molecular spacer and solution ionic  ... 
doi:10.1007/s00249-021-01558-w pmid:34245338 pmcid:PMC8272448 fatcat:z2svytd4kbamrmvarle4lxwtxm

第56回年会予稿集

2018 Seibutsu Butsuri  
In this symposium, each speaker will make 10-minute presentation followed by 3-minute discussion as the second round of screening.  ...  Up to five awardees of the Early Career Award in Biophysics will be selected and announced at the banquet 3SEA-5 光遺伝子操作による単一の糸球体の活性により誘因される恐怖行動の探索 Immobility responses are induced by photoactivation of  ...  We utilize time-lapse microscopy, single-cell tracking algorithms, and microfluidics to control the local environment, image, and analyze the first stages of biofilm formation and to compare the strategies  ... 
doi:10.2142/biophys.58.s1 fatcat:6slkwnu64fen7cjixehxrcmdzm

Volume 43 Number 1

2022 Microbiology Australia  
time (TAT) and improved workload management.  ...  The automation of molecular methods released the technology to be able to be decentralised and provide greater local access and community benefits from the technology and providing equity of community  ...  Video-microscopy of microbes in carefully controlled arenas enables collection of vast quantities of data, for example in the form of high-speed movies, time-lapse photographs, or fluorescence intensity  ... 
doi:10.1071/mav43n1 fatcat:6ch67l3cong2ffwhldc3zolgsy

第54回年会予稿集

2016 Seibutsu Butsuri  
single-cell time-lapse microscopy.  ...  It is an established view that heterochromatic regions in cell nuclei are highly dense. But how really dense are they? What are they like in live cells?  ...  ) 3Pos065 Ohba, Yusuke (大場 雄介) 3SAA-03 Ohgita, Takashi (扇田 隆司) 3Pos072 Ohira, Ayaka (大平 彩花) 1Pos257 Ohki, Mio (大木 規央) 3Pos076 Ohkoshi, A.  ... 
doi:10.2142/biophys.56.s1 fatcat:jxxuc3tfpbhc5isjcmprzec2gi

Abstract Book, XIII International Congress of Histochemistry and Cytochemistry ICHC2008, Imaging of Cell Dynamics, 23rd-27th August 2008, Medical University of Gdansk, Poland

FHC Redaction
2008 Folia Histochemica et Cytobiologica  
Computation of tissue displacement fields using the motion of ECM fibrils as in situ markers for tissue driftwhile simultaneously tracking individual endothelial cells in the same time -allows calculation  ...  Our novel computational time-lapse software and algorithms are designed to quantify large-scale tissue drift and deformations in intact, warm-blooded, quail embryos.  ...  for adaptive mechanisms of tissues and cells.  ... 
doi:10.5603/4388 doaj:cdebe86bf2604301b2401e99ae9a504c fatcat:7j6nazvgnjf2bhooy5awdzq7uy
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