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Motion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy
2012
Computational and Mathematical Methods in Medicine
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This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. ...
The tasks of motion analysis include detection and tracking of objects, as well as analysis of motion behavior, living activity, events, motion statistics, and so forth. ...
However, reconstruction of subcellular structures from images remains a major challenge in the field [19] . ...
doi:10.1155/2012/859398
pmid:22162725
pmcid:PMC3227432
fatcat:pjlhy4ehbzfp5i3lhctqhwfvji
Intravital imaging of cardiac function at the single-cell level
2014
Proceedings of the National Academy of Sciences of the United States of America
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Here we describe a microscopy method for subcellular resolution and motion-artifact-free imaging of contracting cardiomyocytes in vivo in the native mouse heart. ...
cardiac gating approach using cardiac pacing, and (iv) an image processing algorithm, prospective sequential segmented microscopy (PSSM), for motion-artifact-free subcellular imaging at any point in the ...
The project was funded by National Institutes of Health (NIH) Contracts HHSN268201000044C and R01EB006432. A.D.A. was funded by NIH Training Grant T32HL094301. ...
doi:10.1073/pnas.1401316111
pmid:25053815
pmcid:PMC4128110
fatcat:vle26ngxzzeprbbixafga3ybbu
A framework for generating realistic synthetic sequences of total internal reflection fluorescence microscopy images
2013
2013 IEEE 10th International Symposium on Biomedical Imaging
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Since generation of reliable ground truth annotation of fluorescence microscopy sequences is usually a laborious and expensive task, many proposed detection and tracking methods have been evaluated using ...
In this paper, we present a framework for generating realistic synthetic sequences of total internal reflection fluorescence microscope (TIRFM) through simulation of the image formation process and accurate ...
Fluorescence microscopy images are distorted by two main sources of noise; namely, photon noise (Poisson) and readout noise (Gaussian). ...
doi:10.1109/isbi.2013.6556436
dblp:conf/isbi/RezatofighiPGHMHB13
fatcat:k2n4aznfknbf3hdaom7j5kmykm
Fluorescence microscopic imaging and image analysis of the cytoskeleton
2010
2010 Conference Record of the Forty Fourth Asilomar Conference on Signals, Systems and Computers
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The dynamic behaviour of these subcellular structures in living cells can be analysed by fluorescence microscopy yielding series of 2D or 3D images. ...
Towards a quantitative analysis, we present methods for the segmentation and motion estimation of cytoskeletal filaments as well as for the tracking of adhesion sites, allowing the quantification of cytoskeletal ...
ACKNOWLEDGMENTS The work was supported in part by the German Research Council (WI 731/6-1) and by the excellence initiative of the German federal and state governments. ...
doi:10.1109/acssc.2010.5757755
fatcat:le6bqjc62rc2vlrxabimfajhwa
Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures
2014
Light: Science & Applications
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In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescence and SEM images. ...
This paper presents an affine scale-invariant feature transform (ASIFT) based method for correlating SEM images and fluorescence microscopy images. ...
Fluorescence imaging permits convenient visualization of subcellular structures, many of which are not contrasted by SEM imaging. ...
doi:10.1038/lsa.2014.105
fatcat:yhilxd6jsrgchfl4wcvv7yc6oy
Particle Filtering for Multiple Object Tracking in Molecular Cell Biology
2006
2006 IEEE Nonlinear Statistical Signal Processing Workshop
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Motion analysis of subcellular structures in living cells is currently a major topic in molecular cell biology, for which computerized methods are desperately needed. ...
In this paper we adopt and tailor particle filtering techniques for this purpose and present the results of robust and accurate tracking of multiple objects in real fluorescence microscopy image data acquired ...
Motion analysis of subcellular structures such as microtubules or proliferating cell nuclear antigen-like proteins ( Fig. 1 ) requires tracking of hundreds of bright spots in noisy image sequences [1 ...
doi:10.1109/nsspw.2006.4378836
fatcat:hyc7b766ejenfbcmapidegg3oy
A Guided Tour of Selected Image Processing and Analysis Methods for Fluorescence and Electron Microscopy
2016
IEEE Journal on Selected Topics in Signal Processing
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Microscopy imaging, including fluorescence microscopy and electron microscopy, has taken a prominent role in life science research and medicine due to its ability to investigate the 3D interior of live ...
In this paper, we present recent advances in fluorescence and electron microscopy and we focus on dedicated image processing and analysis methods required to quantify phenotypes for a limited number but ...
The authors thank the members of the "Space-time imaging of cellular dynamics of organelles and endomembranes" research team (UMR 144 CNRS Institut Curie) and S. ...
doi:10.1109/jstsp.2015.2505402
fatcat:fgbrxtuhsfbobluogllqll6q2e
Imaging In Vitro on the Nanometer Scale with Ultrasound
2018
Microscopy Today
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By controlling amplitude and detecting frequency, or phase, they produced an image dependent on the out-of-phase motion of the AFM probe. ...
EBSD 2018 -Electron Backscatter Diffraction Conference There have been impressive advances in recent years in electron microscopy and light microscopy, but imaging molecular structures in vitro under physiological ...
doi:10.1017/s1551929518000214
fatcat:cvkgtnugwrdhbps7wfsbnupr4e
Combining optical tweezing and confocal microscopy for the study of cell mechanics
2007
2007 Conference on Lasers and Electro-Optics - Pacific Rim
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We have developed a system combining an optical tweezers and a confocal microscope for the study of cell mechanics. ...
In passive microrheology, tracer particles are embedded into the cell, and their Brownian motion is tracked using either a laser beam and a quadrant detector, or fluorescence video microscopy and a multiple ...
The limitation of measuring the bulk deformation of a cell is that it does not resolve the mechanical behavior on a subcellular level. ...
doi:10.1109/cleopr.2007.4391166
fatcat:x4ngh2snozf47ilnn5c4xr7g74
High Resolution Intravital Imaging of Subcellular Structures of Mouse Abdominal Organs Using a Microstage Device
2012
PLoS ONE
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We demonstrate that this technique allows for the quantitative analysis of subcellular structures and gene expressions in cells, the tracking of intracellular processes in realtime as well as three-dimensional ...
Intravital imaging of brain and bone marrow cells in the skull with subcellular resolution has revolutionized neurobiology, immunology and hematology. ...
Kiyotsugu Kojima of Olympus Corporation for microscopy technical support, Dr. ...
doi:10.1371/journal.pone.0033876
pmid:22479464
pmcid:PMC3313950
fatcat:2sl5tw3rn5dw7hferlifi6q32q
A new detection scheme for multiple object tracking in fluorescence microscopy by joint probabilistic data association filtering
2008
2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro
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Index Terms-Kalman filtering, Bayesian estimation, multiple object tracking, fluorescence microscopy. ...
Tracking of multiple objects in biological image data is a challenging problem due largely to poor imaging conditions and complicated motion scenarios. ...
INTRODUCTION Current biological studies using time-lapse fluorescence microscopy imaging require analysis of huge amounts of image data. ...
doi:10.1109/isbi.2008.4540983
dblp:conf/isbi/SmalNM08
fatcat:h3j543pfubbnljpjw3vzvv36qy
Digital correction of motion artefacts in microscopy image sequences collected from living animals using rigid and nonrigid registration
2011
Journal of Microscopy
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Intravital microscopy introduces the additional problem of motion artifacts, resulting from respiratory motion and heartbeat from specimens imaged in vivo. ...
Digital image analysis is a fundamental component of quantitative microscopy. However, intravital microscopy presents many challenges for digital image analysis. ...
O'Brien Award from the National Institutes of Health NIH/NIDDK P50 DK 61594. ...
doi:10.1111/j.1365-2818.2011.03557.x
pmid:22092443
pmcid:PMC3856233
fatcat:xcporyoroncllmua3kv7jrqs6m
Imaging and Optical Microscopy - III
2008
Biophysical Journal
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(TIRF), and confocal laser scanning microscopy (CLSM) allow researchers to obtain images of fluorescently labeled components of cell membranes. ...
The technique does not rely on spatial correlations, which frees it from biases due to subcellular compartmentalization that can be problematic for spatial image correlation based approaches. ...
Imaging Subcellular Structure by Using 4Pi Confocal Fluorescence Microscopy Karen Deuschle, Sylvia Glaschick, Carlheinz R€ ocker, Franz Oswald, Joerg Wiedenmann, Gerd U. ...
doi:10.1016/s0006-3495(08)79032-5
fatcat:ojsdoxw6qbamhlu6va5oe23amu
Dynamic super-resolution structured illumination imaging in the living brain
2019
Proceedings of the National Academy of Sciences of the United States of America
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We optimized image acquisition and reconstruction to combat sample motion and applied adaptive optics to correcting sample-induced optical aberrations in super-resolution structured illumination microscopy ...
However, to apply it to in vivo brain imaging, we must address the challenges of 3D imaging in an optically heterogeneous tissue that is constantly in motion. ...
We thank Rongwen Lu for his assistance with the initial design of the optical setup and David P. Hoffman for helpful discussions. The Howard Hughes Medical Institute supported this work. ...
doi:10.1073/pnas.1819965116
pmid:31028150
pmcid:PMC6511017
fatcat:n64eumwekffr3lqxj3cwfx44ie
Short Communication: Subcellular Motion Compensation for Minimally Invasive Microscopy, In Vivo: Evidence for Oxygen Gradients in Resting Muscle
2010
Circulation Research
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A primary limitation of optical microscopy in vivo is tissue motion, which prevents physiological time course observations or image averaging. ...
Rationale: In vivo microscopy seeks to observe dynamic subcellular processes in a physiologically relevant context. ...
(D) Lookup table of percentage change image from +100% (top) to -100% (bottom). Net motion, filtered image data, and survey of percent change (A) Graph of total motion, ! ! ...
doi:10.1161/circresaha.109.211946
pmid:20167928
pmcid:PMC3209509
fatcat:dyg3jj6ukzg4tp2opy6slezkcm
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