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Solution structure and thermal stability of ribosomal protein L30e from hyperthermophilic archaeonThermococcus celer

Kam-Bo Wong, Chi-Fung Lee, Siu-Hong Chan, Tak-Yuen Leung, Yu Wai Chen, Mark Bycroft
2003 Protein Science  
The conformational stability of T. celer L30e was measured by guanidine and thermal-induced denaturation, and compared with that obtained for yeast L30e, a mesophilic homolog.  ...  Sequence-structure comparison of homologous proteins from thermophilic and mesophilic origins provides insights on the structural basis of thermostability of proteins.  ...  by payment of page charges.  ... 
doi:10.1110/ps.0302303 pmid:12824494 pmcid:PMC2323938 fatcat:k7royftrpjbmtc3jeppe6nlgle

Protein Thermostability Is Owing to Their Preferences to Non-Polar Smaller Volume Amino Acids, Variations in Residual Physico-Chemical Properties and More Salt-Bridges

Anindya Sundar Panja, Bidyut Bandopadhyay, Smarajit Maiti, Surajit Bhattacharjya
2015 PLoS ONE  
Methods Presently, a total 400 (200 thermophilic and 200 mesophilic homologue) proteins were studied utilizing several software/databases to evaluate their amino acid preferences.  ...  Introduction Protein thermostability is an important field for its evolutionary perspective of mesophilic versus thermophilic relationship and for its industrial/ therapeutic applications.  ...  VMD was utilized in this study to animate and analyze the trajectory of a molecular dynamics (MD) simulation.  ... 
doi:10.1371/journal.pone.0131495 pmid:26177372 pmcid:PMC4503463 fatcat:cgwzudepgjbyfmd6gz3tsec65i

Abstracts of the 29th Annual Symposium of The Protein Society

2015 Protein Science  
Finally, 19F-NMR was used to study solvent exposure of the complex and its dynamics in solution through relaxation dispersion of the 19F-nucleus at different temperatures.  ...  We employ an optimized site-specific and inducible split-protein protease to examine the role of a classic apoptotic node, the Caspase Activated DNase (CAD).  ...  TraI is a 1756 residual protein and contains 3 major domains: N-terminal relaxase domain, a central helicase domain and a C terminal domain (CTD).  ... 
doi:10.1002/pro.2823 pmid:26452528 pmcid:PMC4632850 fatcat:ejmksoqj3zbsrkabl4jyp7emgi