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Fast wide-volume functional imaging of engineered in vitro brain tissues

G. Palazzolo, M. Moroni, A. Soloperto, G. Aletti, G. Naldi, M. Vassalli, T. Nieus, F. Difato
2017 Scientific Reports  
Furthermore, we implemented a 3D co-culture system mimicking the contiguous interfaces of distinct brain tissues such as the cortical-hippocampal interface.  ...  Herein, we propose an experimental model based on 3D neuronal networks in an alginate hydrogel, a tunable wide-volume imaging approach, and an efficient denoising algorithm to resolve, down to single cell  ...  Acknowledgements We thank Marina Nanni for the technical assistance in cell culture preparation, Alessandro Parodi and Giacomo Pruzzo for the development of electronic and software interfaces to perform  ... 
doi:10.1038/s41598-017-08979-8 pmid:28819205 pmcid:PMC5561227 fatcat:twme6t7pzfd3ligmnaa2oa4wgm

Self-Organized Criticality in the Brain

Dietmar Plenz, Tiago L. Ribeiro, Stephanie R. Miller, Patrick A. Kells, Ali Vakili, Elliott L. Capek
2021 Frontiers in Physics  
This rich dynamical repertoire is not observed in dissociated cortex cultures, which lack the differentiation into cortical layers and exhibit a dynamical phenotype expected for a first-order phase transition  ...  a slope of −3/2 and a critical branching parameter of 1.  ...  Chialvo and Patrick Kanold for comments and support during writing of this manuscript.  ... 
doi:10.3389/fphy.2021.639389 fatcat:wrvcvfvw4vcvxm6rr5usohyptq

Self-Organized Criticality in the Brain [article]

Dietmar Plenz, Tiago L. Ribeiro, Stephanie R. Miller, Patrick A. Kells, Ali Vakili, Elliott L. Capek
2021 arXiv   pre-print
branching parameter of 1.  ...  This rich dynamical repertoire is not observed in dissociated cortex cultures, which lack cortical layers and exhibit dynamics similar to a 1st-order phase transition.  ...  Chialvo and Patrick Kanold for comments and support during writing of this manuscript.  ... 
arXiv:2102.09124v2 fatcat:ullxgm6lqfcdjeaptwzypcliry

Evaluation of in vitro neuronal networks for the study of spontaneous activity

Diletta Pozzi
2020 STEMedicine  
This review provides an overview of in vitro models for the study of spontaneous network activity, discussing their ability to reproduce in vivo - like dynamics and the main findings obtained with each  ...  While explanted brain slices are able to reproduce the neuronal oscillations typically observed in anaesthetized animals, dissociated cultures allow the use of patient-derived neurons and limit the number  ...  Recent studies showed some differences in spontaneous, single-unit spike activity between dissociated neurons and awake animals: in vitro, neurons were more likely to fire in synchronized bursts; while  ... 
doi:10.37175/stemedicine.v1i2.35 fatcat:n3qfnx4f7bhavkgjddt4hdyhau

Simulations of cortical pyramidal neurons synchronized by inhibitory interneurons

W. W. Lytton, T. J. Sejnowski
1991 Journal of Neurophysiology  
The parameters were then adjusted to produce repetitive bursting similar to that seen in some cortical pyramidal cells entrained during visual stimulation. 3.  ...  Phase-locking with IPSPs in these models was possible because the IPSPs could cause either an increase or a decrease in firing rate over a limited range.  ...  Douglas for providing us with the cortical cell morphology; and F. Crick, C. Koch, C. Gray, and P. Bush for many helpful discussions. The comments and suggestions of G.  ... 
doi:10.1152/jn.1991.66.3.1059 pmid:1661324 fatcat:la6hufecvbb65c62gmv5uvk7jy

Plasticity of recurring spatiotemporal activity patterns in cortical networks

Radhika Madhavan, Zenas C Chao, Steve M Potter
2007 Physical Biology  
To study the potential role of bursts of action potentials in memory mechanisms, we investigated patterns of spontaneous multi-single-unit activity in dissociated rat cortical cultures in vitro.  ...  transfer and the expression of memories stored in cortical networks.  ...  Easy manipulation and enhanced accessibility of dissociated cortical networks make MEA cultures the ideal platform to explore the effects of varied input patterns on the structure of output activity patterns  ... 
doi:10.1088/1478-3975/4/3/005 pmid:17928657 pmcid:PMC2577584 fatcat:6er6mexsvzaclcgpxulzvxdonm

Slow oscillating population activity in developing cortical networks: models and experimental results

Thomas Baltz, Andreas Herzog, Thomas Voigt
2011 Journal of Neurophysiology  
Here we study the emergence of the oscillatory activity in three computational models and multisite extracellular recordings that we obtained from developing cortical networks in vitro.  ...  Intrinsically bursting neurons were present in cortical cultures, but we found them only in those cultures that were younger than 3 wk in vitro.  ...  DISCLOSURES The authors declare that this research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.  ... 
doi:10.1152/jn.00889.2010 pmid:21697440 fatcat:sxg7cqwvvbhtfbgumtk5ujhhce

Towards animal-free neurotoxicity screening: Applicability of hiPSC-derived neuronal models for in vitro seizure liability assessment

Anke Tukker
2019 ALTEX: Alternatives to Animal Experimentation  
Importantly, compared to rat primary cortical neurons, the hiPSC-derived neuronal models were equally, or even better in the case of 4-AP, suited to detect seizurogenicity.  ...  However, the sensitivity and degree of chemical-induced effects differs between the models, which can likely be explained by differences in seeding density, maturation and different ratios of inhibitory  ...  Acknowledgements We gratefully acknowledge members of the Neurotoxicology Research Group for helpful discussions.  ... 
doi:10.14573/altex.1907121 pmid:31686111 fatcat:lwmqt5uberfr7oyx5ajkns2xyu

Plasticity and Adaptation in Neuromorphic Biohybrid Systems

Richard George, Michela Chiappalone, Michele Giugliano, Timothée Levi, Stefano Vassanelli, Johannes Partzsch, Christian Mayr
2020 iScience  
Moreover, the use of biologically plausible learning rules allows forming an overall fault-tolerant system of co-developing subsystems.  ...  The recent years have seen both advances in the theoretical aspects of spiking neural networks for their use in classification and control tasks and a progress in electrophysiological methods that is pushing  ...  ., and C.M.) and project ''IN-FET'' (GA n. 862882 to M.G.).  ... 
doi:10.1016/j.isci.2020.101589 pmid:33083749 pmcid:PMC7554028 fatcat:a7iwoyllezdwbor5chqfym45pq

Interactions Between Membrane Conductances Underlying Thalamocortical Slow-Wave Oscillations

A. DESTEXHE, T. J. SEJNOWSKI
2003 Physiological Reviews  
National Institutes of Health.  ...  This research was supported by the Centre National de la Recherche Scientifique, the Medical Research Council of Canada, the Human Frontier Science Program, the Howard Hughes Medical Institute, and the  ...  The failure of a Hodgkin-Huxley model to simulate these properties could reflect an incomplete knowledge of the biophysical parameters of the I T because of errors in voltage-clamp measurements.  ... 
doi:10.1152/physrev.00012.2003 pmid:14506309 pmcid:PMC2927823 fatcat:jyipjjzhxrbfffmla5rhoxltay

Quantifying network properties in multi-electrode recordings: spatiotemporal characterization and inter-trial variation of evoked gamma oscillations in mouse somatosensory cortex in vitro

Cristian Carmeli, Paolo Bonifazi, Hugh P. C. Robinson, Michael Small
2013 Frontiers in Computational Neuroscience  
Bursts of gamma oscillatory activity lasting a few hundred milliseconds could be initiated only by brief trains of electrical stimulations applied at the deepest cortical layers and simultaneously delivered  ...  Pair-wise differences in the oscillation phase were used to determine the presence of instantaneous synchronization between the different sites of the circuitry during the oscillatory period.  ...  Supported by an EC FP6 research grant (#12788), and a Hong Kong UGC CERG grant (PolyU 5262/11E). Michael Small is supported by an Australian Research Council Future Fellowship (FT110100896).  ... 
doi:10.3389/fncom.2013.00134 pmid:24137127 pmcid:PMC3797413 fatcat:o2gixi3zyvgcljtcfp23wrqoyi

A Maximum Entropy Model Applied to Spatial and Temporal Correlations from Cortical Networks In Vitro

A. Tang, D. Jackson, J. Hobbs, W. Chen, J. L. Smith, H. Patel, A. Prieto, D. Petrusca, M. I. Grivich, A. Sher, P. Hottowy, W. Dabrowski (+2 others)
2008 Journal of Neuroscience  
This suggested that temporal dependencies are a common feature of cortical network activity, and should be considered in future models.  ...  The model worked slightly less well in cortex than in retina, accounting for 88 Ϯ 7% (mean Ϯ SD) of network correlations.  ...  To explore the first issue, we applied a second-order maximum entropy model to a wide variety of in vitro cortical networks, including acute slices from rat and human tissue, as well as organotypic and  ... 
doi:10.1523/jneurosci.3359-07.2008 pmid:18184793 fatcat:lqjmadahojdjlncgvm6yol7g74

Population based models of cortical drug response: insights from anaesthesia

Brett L. Foster, Ingo Bojak, David T. J. Liley
2008 Cognitive Neurodynamics  
plausible dynamical models of cortical tissue.  ...  Such theoretical models allow exploration of neural dynamics, in particular their modification by drug action.  ...  Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided  ... 
doi:10.1007/s11571-008-9063-z pmid:19003456 pmcid:PMC2585619 fatcat:nb7lpj7q2vg35bhpikhtrhcw3i

Spatiotemporal Memory Is an Intrinsic Property of Networks of Dissociated Cortical Neurons

H. Ju, M. R. Dranias, G. Banumurthy, A. M. J. VanDongen
2015 Journal of Neuroscience  
We have explored the spatiotemporal information-processing capability of networks formed from dissociated rat E18 cortical neurons growing in culture.  ...  These results show that the ability to process and integrate complex spatiotemporal information is an intrinsic property of generic cortical networks that does not require specifically designed circuits  ...  Cultured networks grown from dissociated cortical neurons provide a model system to answer this question.  ... 
doi:10.1523/jneurosci.3793-14.2015 pmid:25740531 fatcat:lyw5jj7vcbebpjothjfs6r35ua

Long-term Relationships between Cholinergic Tone, Synchronous Bursting and Synaptic Remodeling

Maya Kaufman, Michael A. Corner, Noam E. Ziv, Anna Dunaevsky
2012 PLoS ONE  
Subsequent blockage of cholinergic receptors led to an immediate restoration of synchronous bursting and the gradual reversal of synaptic growth.  ...  Finally, the reemergence of synchronous bursting in the presence of elevated cholinergic tone indicates that the capacity of cholinergic neuromodulation to indefinitely suppress synchronous bursting might  ...  A second, more general concern relates to the model system used here, that is, networks of dissociated rat cortical neurons maintained in primary culture.  ... 
doi:10.1371/journal.pone.0040980 pmid:22911726 pmcid:PMC3402441 fatcat:sgsp6vyy5bgahh2qcd7wxclm4i
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