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Model-based design and analysis of a reconfigurable continuous-culture bioreactor

Luan Viet Nguyen, Eric J. Nelson, Amol Vengurlekar, Ruoshi Zhang, Kristopher I. White, Victor Salinas, Taylor T. Johnson
2014 Proceedings of the 4th ACM SIGBED International Workshop on Design, Modeling, and Evaluation of Cyber-Physical Systems - CyPhy '14  
Depending on the particular experiment, the reconfigurable continuous-culture bioreactor we model and analyze may operate as: (a) a chemostat with constant volume, (b) a turbidostat with constant bacterial  ...  In this paper, we present a model-based design and analysis of prototype laboratory equipment used for growing bacteria under precisely controlled conditions for systems biology experiments.  ...  CONCLUSION AND FUTURE WORK In this paper, we present a model-based design and analysis of a continuous-culture apparatus.  ... 
doi:10.1145/2593458.2593469 dblp:conf/cyphy/NguyenNVZWSJ14 fatcat:vammi7tjovfovdj26bbtn4lyky

Single-Use Systems in Animal Cell-Based Bioproduction [chapter]

William G. Whitford
2012 Antibody-Mediated Drug Delivery Systems  
Process Focus: UpstreAm prodUction And downstreAm processing Who should read: process development, mAnUfActUring, fAcility design/engineering, operAtions  ...  Due to the proximity of downstream disposable elements to final products, risk-analysis may indicate a heightened requirement for testing-based validation and documentation.  ...  Processes and models in infancy: based on preliminary data Many publications, filings, and case studies are appearing.  ... 
doi:10.1002/9781118229019.ch11 fatcat:fyyj5azjhjdvbgipahuf5m2zfe

Ex vivo Manufactured Neutrophils for Treatment of Neutropenia—A Process Economic Evaluation

Mario A. Torres-Acosta, Richard P. Harrison, Elizabeth Csaszar, Marco Rito-Palomares, Marion E. G. Brunck
2019 Frontiers in Medicine  
The model indicates that the most cost-efficient culture volume (batch size) is ~100 L in a single bioreactor.  ...  A protocol has been developed that produces clinical-scale quantities of neutrophils from hematopoietic stem and progenitor cells (HSPC) in 10 L single-use bioreactors (1).  ...  Brad Spellberg for insightful comments, and to GE Healthcare, CanPeptide, Miltenyi Biotec, Sartorius Stedim, and Sigma-Aldrich, for providing insight into manufacturing costs.  ... 
doi:10.3389/fmed.2019.00021 pmid:30881955 pmcid:PMC6405517 fatcat:vfak3ydvefdopfxpk3iyjnecwu

Development of a Novel Bioreactor for the Mechanical Loading of Tissue-Engineered Heart Muscle

R.K. Birla, Y.C. Huang, R.G. Dennis
2007 Tissue engineering  
A custom bioreactor system was designed using SolidWorks (Concord, MA) and structural components were manufactured using fusion deposition modeling.  ...  Conclusions: In this study, we demonstrate compatibility of a novel bioreactor system with BEHMs and the stability of the BEHMs in response to stretch protocols.  ...  Bioreactor development was supported by a contract from the Defense Advanced Research Projects Agency (DARPA) to R.G. Dennis (Award Number FA9550-05-1-0015).  ... 
doi:10.1089/ten.2006.0359 pmid:17590151 fatcat:nroir5cc75esdem75mr237z7oy

Modeling nutrient consumptions in large flow-through bioreactors for tissue engineering

Mamatha Devarapalli, Benjamin J. Lawrence, Sundararajan V. Madihally
2009 Biotechnology and Bioengineering  
The fundamental concepts in designing these bioreactors for regenerating large high aspect ratio tissues (large surface area relative to the thickness of the matrix such as skin, bladder, and cartilage  ...  Further, tissue regeneration is a dynamic process where the porous characteristics change due to proliferation of cells, de novo deposition of matrix components, and degradation of the porous architecture  ...  Acknowledgments Financial support was provided by the Oklahoma Center for Advancement of Scienceaa and Technology (HR05-075), and National Institutes of Health (1R21DK074858-01A2).  ... 
doi:10.1002/bit.22333 pmid:19422037 pmcid:PMC2730170 fatcat:w3ezauwyl5ar3mm265lkfmpqkq

A MODULAR PHOTOSYNTHETIC MICROBIAL FUEL CELL WITH INTERCHANGEABLE ALGAE SOLAR COMPARTMENTS [article]

Daniel Fleury
2017 bioRxiv   pre-print
The analysis of data indicates that Algae-based MFCs are proven hopeful for alternative high-yield energy production.  ...  The optimal electric levels occurred at 286 A (absorbance) and 35 mW- Nannochloropsis, 123 A- Chlorella (30.2 mW), and 142 A (31 mW)- Spirulina culture.  ...  All parameters in each small-scale photo-bioreactor was regulated Algae Culturing Methods: Figure 5 : 5 A), Nannochloropsis 286 (A), and 142 (A) Physical data analysis of Algal development (in individual  ... 
doi:10.1101/166793 fatcat:q2eltkkarrbthenan3datgsicm

Lab on a Chip: Bioreactors miniaturization for rapid optimization of biomedical processes and its impact on SARS-CoV-2 diagnosis

C.P. Ortega, D.A Corredor, M.E Santillán, W.S Ger, J.M Noceda, J.M Pais-Chanfrau, L.E Trujillo
2021 Bionatura  
Lab on a Chip (LoC) as part of Microbioreactors (MBRs) constitute an emergent technology to carry out micro-bioprocesses based on microfluidics research.  ...  As a result, a beneficial point of advancement is reached, producing useful consumables for humanity.  ...  invaluable help and guidance during the development of this work.  ... 
doi:10.21931/rb/2021.06.03.31 fatcat:4nylfhfo55gztg4y4wdj5hr2xm

Microbioreactors for Bioprocess Development

Z ZHANG, G PEROZZIELLO, P BOCCAZZI, A SINSKEY, O GESCHKE, K JENSEN
2007 Journal of the Association for Laboratory Automation  
kinetics, genome-wide expression analysis, pH control, and even continuous culture of bacterial cells.  ...  Conclusions A 150 J-lL membrane-aerated and actively mixed microbioreactor was designed and fabricated for continuous culture of microbial cells.  ... 
doi:10.1016/j.jala.2006.10.017 fatcat:7nk2jbuih5evnebmei4ljqnnnq

Bioprocessing of Avian Influenza VLP Vaccine using Baculovirus-Insect Cell Expression System

Matthew Chrisdianto, Fedric Intan Damai, Roselyn Mulyono, Jesslyn Audrey Virginia, Katherine K
2022 Indonesian Journal of Life Sciences  
The upstream process consists of the IC infection by the BV and BV-IC cell cultivation inside the bioreactor.  ...  The objective of this review is to assess the production of the avian influenza vaccine (i.e., H5N1 and H7N9 strains) made from VLP by utilizing a baculovirus-insect cell (BV-IC) expression system.  ...  (Indonesia International Institute for Life Sciences), for her assistance in the completion of this review.  ... 
doi:10.54250/ijls.v4i1.69 fatcat:s4k5l53l7fesbdyyfdgkzzggw4

What Has History to Do with Cognition? Interactive Methods for Studying Research Laboratories

Elke Kurz-Milcke, Nancy Nersessian, Wendy Newstetter
2004 Journal of Cognition and Culture  
We portray the prevalent design-orientation in this engineering specialty and how the prevailing activity of cell-culturing in these labs transitions into a design activity for the bio-medical engineers  ...  model-based reasoning.  ...  He continued by pulling down previous versions of bioreactors (made by earlier researchers as well) and explaining the modifications and problems for which design changes were made.  ... 
doi:10.1163/1568537042484959 fatcat:i2uom6lm5jbetbs3svcttiasea

Bioengineering Methods for Analysis of Cells In Vitro

Gregory H. Underhill, Galie Peter, Christopher S. Chen, Sangeeta N. Bhatia
2012 Annual Review of Cell and Developmental Biology  
We also describe the mechanistic insights gained by the use of these novel tools, with associated applications ranging from fundamental biological studies, in vitro modeling of in vivo processes, and cell-based  ...  These approaches have emphasized the reduction of complex multicomponent cellular microenvironments into distinct individual signals as a means to both (a) better construct mimics of in vivo microenvironments  ...  Building on systems-based modeling of intercellular interactions and bioreactor conditions, modifications in the bioreactor design could be employed and were demonstrated to enhance stem cell proliferation  ... 
doi:10.1146/annurev-cellbio-101011-155709 pmid:23057744 fatcat:5s6a4gaulffqti64ab5mma765i

Development of a multiplexed microbioreactor system for high-throughput bioprocessing

Nicolas Szita, Paolo Boccazzi, Zhiyu Zhang, Patrick Boyle, Anthony J. Sinskey, Klavs F. Jensen
2005 Lab on a Chip  
The microbioreactors are fabricated out of poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS), and have a working volume of 150 ml.  ...  The profiles for OD, DO, and pH compare favourably to fermentations performed in bioreactor systems with multiple bench-scale reactors.  ...  Chen for her contribution to the first design of the multiplexed microbioreactor system. The authors also thank Andrea Zanzotto for reading the manuscript.  ... 
doi:10.1039/b504243g pmid:16027932 fatcat:fu6xgzd7szfylnhvnlzjxnraba

Agent-Based Control System for Sustainable Wastewater Treatment Process [chapter]

Grzegorz Polaków, Mieczyslaw Metzger
2012 Lecture Notes in Computer Science  
Additional functionalities of the control system include the support for a cooperation between multiple experimenters, and on-line real-time modelling of the system providing the aid in a decision making  ...  The implemented control system is described, which is based on a real-time agent communication protocol implementing a blackboard knowledge system.  ...  N N514 471539 and by the Ministry of Science and Higher Education under grant BK-UiUA. The authors would like to thank the anonymous reviewer for his extensive and helpful suggestion and comments.  ... 
doi:10.1007/978-3-642-35455-7_19 fatcat:zhqyc4rmp5dototnjcowzvn7oi

Model Reference Adaptive Control for Milk Fermentation in Batch Bioreactors

Jožef Ritonja, Andreja Goršek, Darja Pečar
2020 Applied Sciences  
Firstly, the paper presents the derivation of the enhanced mathematical model suitable for developing a control system. A linearisation and eigenvalue analysis of this model were made.  ...  Batch bioreactors do not allow the inflow/outflow of substances during operation. Therefore, we have developed a control system based on a stirrer drive instead of material flow.  ...  Reference [12] presents the design and analysis of adaptive and robust-adaptive control strategies for a class of anaerobic wastewater treatment processes.  ... 
doi:10.3390/app10249118 fatcat:xmxlmmz4enfiho67vrrgmzlvny

Design and Fabrication of Low-Cost Microfluidic Chips and Microfluidic Routing System for Reconfigurable Multi-(Organ-on-a-Chip) Assembly

Sadeq Abu-Dawas, Hawra Alawami, Mohammed Zourob, Qasem Ramadan
2021 Micromachines  
of fitting a variety of (organ-on-a-chip)-based biological models with multicell architectures.  ...  A low-cost, versatile, and reconfigurable fluidic routing system and chip assembly have been fabricated and tested.  ...  Conclusions In this paper, we have presented the design, fabrication, and characterization of a low-cost microfluidic chip and a fluidic routing unit that enables custom and reconfigurable chip assembly  ... 
doi:10.3390/mi12121542 pmid:34945392 pmcid:PMC8707086 fatcat:m4qr7svjnbb7rgyim7eh3mkcvm
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