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NBCe1A dimer assemble visualized by bimolecular fluorescence complementation

Min-Hwang Chang, An-Ping Chen, Michael F. Romero
2014 AJP - Renal Physiology  
NBCe1A dimer assemble visualized by bimolecular fluorescence complementation.  ...  The S427L-E91R heterodimer complex allows the monomers to structurally complement each other resulting in a dimer with wild-type like function.  ...  Fluorescent signals reflect dimer formation when we coexpressing mix matched YFP N -NBCe1A and YFP C -NBCe1A pair in the oocytes (A).  ... 
doi:10.1152/ajprenal.00284.2013 pmid:24477681 pmcid:PMC3949041 fatcat:cindt3ubkfb6jbgyoovul3pgia

An Enveloped Auricular Prosthesis for Ear Defect: A Clinical Report

Nadira Hatim., Salem Hazem, Mohammed Abdulla
2011 Al-Rafidain Dental Journal  
) the color of the mix match with the color of the contra-lateral ear (Fig.10) .  ...  The purpose of this report to resolve a special design to envelope the defect and construct the acceptable retention of artificial ear.  ... 
doi:10.33899/rden.2011.164434 fatcat:kekvt23f7fbg3fwg42btzdihny

Chitosan-based nanoformulated (–)-epigallocatechin-3-gallate (EGCG) modulates human keratinocyte-induced responses and alleviates imiquimod-induced murine psoriasiform dermatitis

Jean Christopher Chamcheu, Imtiaz A Siddiqui, Vaqar M Adhami, Stephane Esnault, Dhruba J Bharali, Abiola S Babatunde, Stephanie Adame, Randall J Massey, Gary S Wood, B Jack Longley, Shaker A Mousa, Hasan Mukhtar
2018 International Journal of Nanomedicine  
We also thank Drs G Kerr Whitfield and Kelly A Keating, for helpful discussions and for critically reviewing this manuscript, and Dr Anna V Kiyanova for assistance with nanoparticle size characterization  ...  All authors contributed toward data analysis, drafting, and critically revising the paper, and agree to be accountable for all aspects of the work.  ...  Human mix-matched 6-Plex and mouse 36-Plex ProcartaPlex™ multiplex bead-based immunoassay kits were purchased from eBioscience Affymetrix/eBioscience (Santa Clara, CA, USA).  ... 
doi:10.2147/ijn.s165966 pmid:30057446 pmcid:PMC6059258 fatcat:6ekce55pojhyjoaqq7vgsvilwy

Conditional control of RNA-guided nucleic acid cleavage and gene editing

Shao-Ru Wang, Ling-Yu Wu, Hai-Yan Huang, Wei Xiong, Jian Liu, Lai Wei, Ping Yin, Tian Tian, Xiang Zhou
2020 Nature Communications  
For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects.  ...  This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.  ...  Acknowledgements This study was supported by the National Natural Science Foundation of China (No. 21722803, 91853119, 21572169, 21721005, 91753201, 21877086 and 21672165), the Hubei Natural Science Foundation for  ... 
doi:10.1038/s41467-019-13765-3 pmid:31900392 pmcid:PMC6941951 fatcat:dauahmqpxnhcjgbgwy5yuznplm

Differences in Glycoprotein Complex Receptor Binding Site Accessibility Prompt Poor Cross-Reactivity of Neutralizing Antibodies between Closely Related Arenaviruses

Rachel B. Brouillette, Elisabeth K. Phillips, Natarajan Ayithan, Wendy Maury, Susan R. Ross
2017 Journal of Virology  
A vaccine that protects against a broad range of New World arenaviruses is desirable for purposes of simplicity, cost, and broad protection against multiple National Institute of Allergy and Infectious  ...  To address this, MACV/JUNV chimeric GPCs were assessed for interaction with a group of α-JUNV GPC monoclonal antibodies (MAbs) and mouse antisera against JUNV or MACV GPC.  ...  ACKNOWLEDGMENTS The study was supported by a developmental award to W.M. from a National Institutes of Health (NIH) Regional Center of Excellence grant (U54 AI1057156 [David Walker, principal investigator  ... 
doi:10.1128/jvi.01454-16 pmid:28100617 pmcid:PMC5355595 fatcat:ravji4bkf5g23aeibemfwc3b3e

A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately selective enzymes

Maiko Furubayashi, Mayu Ikezumi, Shinichi Takaichi, Takashi Maoka, Hisashi Hemmi, Takuya Ogawa, Kyoichi Saito, Alexander V Tobias, Daisuke Umeno
2015 Nature Communications  
Here, we describe the assembly of a six-enzyme pathway in Escherichia coli for the synthesis of C 50 -astaxanthin, a non-natural purple carotenoid.  ...  Synthetic biology aspires to construct natural and non-natural pathways to useful compounds.  ...  Recent studies have begun to explore the space of novel metabolites by mixing, matching and laboratory-evolving biosynthetic genes from diverse sources in bacteria and yeasts [2] [3] [4] [5] [6] .  ... 
doi:10.1038/ncomms8534 pmid:26168783 pmcid:PMC4510654 fatcat:oymdu3qmv5duncj4aynl6lnx3u

Virus-Like Particle (VLP) Plus Microcrystalline Tyrosine (MCT) Adjuvants Enhance Vaccine Efficacy Improving T and B Cell Immunogenicity and Protection against Plasmodium berghei/vivax

Gustavo Cabral-Miranda, Matthew Heath, Mona Mohsen, Ariane Gomes, Paul Engeroff, Amy Flaxman, Fabiana Leoratti, Aadil El-Turabi, Arturo Reyes-Sandoval, Murray Skinner, Matthias Kramer, Martin Bachmann
2017 Vaccines  
adhesive protein (TRAP) as a target antigen.  ...  All vaccine formulations using adjuvants and/or VLP increased humoral immunogenicity for PvTRAP compared to the antigen alone.  ...  targets where the mix-/match-combination of new adjuvants are sought.  ... 
doi:10.3390/vaccines5020010 pmid:28468322 pmcid:PMC5492007 fatcat:d2rf7xsbmrhyfdee7avr77vg6y

Mutational Analysis Reveals Distinct Features of the Nox4-p22phoxComplex

Katharina von Löhneysen, Deborah Noack, Algirdas J. Jesaitis, Mary C. Dinauer, Ulla G. Knaus
2008 Journal of Biological Chemistry  
The integral membrane protein p22 phox forms a heterodimeric enzyme complex with NADPH oxidases (Noxs) and is required for their catalytic activity.  ...  These studies highlight the distinct interaction of the key regulatory p22 phox subunit with Nox4, a feature which could provide the basis for selective inhibitor development.  ...  Roos and T. van den Berg for anti-p22 phox antibody. We also thank K. Schreiber for excellent editorial assistance.  ... 
doi:10.1074/jbc.m804200200 pmid:18849343 pmcid:PMC2596391 fatcat:sa7vmcywpzbhhfszjyozasbfuu

Augmented Expansion of Treg Cells From Healthy and Autoimmune Subjects via Adult Progenitor Cell Co-Culture

James L. Reading, Valerie D. Roobrouck, Caroline M. Hull, Pablo Daniel Becker, Jelle Beyens, Alice Valentin-Torres, Dominic Boardman, Estefania Nova Lamperti, Samantha Stubblefield, Giovanna Lombardi, Robert Deans, Anthony E. Ting (+1 others)
2021 Frontiers in Immunology  
Multipotent adult progenitor cells (MAPCⓇ) promote Treg cell differentiation in vitro, suggesting they may be repurposed to enhance ex vivo expansion of Tregs for adoptive cellular therapy.  ...  Here, we use a Good Manufacturing Practice (GMP) compatible Treg expansion platform to demonstrate that MAPC cell-co-cultured Tregs (MulTreg) exhibit a log-fold increase in yield across two independent  ...  Cytokine abundance in the supernatant of cell cultures was measured by the LEGENDplex ™ multi-analyte flow assay kit, human Th cytokine mix & match subpanel (Biolegend).  ... 
doi:10.3389/fimmu.2021.716606 pmid:34539651 pmcid:PMC8442662 fatcat:5j35fwehfndize4lf5v54xodta

Patient Mutations of the Intellectual Disability Gene KDM5C Downregulate Netrin G2 and Suppress Neurite Growth in Neuro2a Cells

Gengze Wei, Xinxian Deng, Saurabh Agarwal, Shigeki Iwase, Christine Disteche, Jun Xu
2016 Journal of Molecular Neuroscience  
Briefly, chromatin was cross-linked with formaldehyde (1.42%) for 15 min at room temperature, followed by a glycine quenching (125 mM, 5 min).  ...  It encodes a histone demethylase that is involved in gene regulation in neuronal differentiation and morphogenesis.  ...  Shigeyoshi Itohara for kindly providing us with the Ntng2 plasmid.  ... 
doi:10.1007/s12031-016-0770-3 pmid:27421841 pmcid:PMC5412133 fatcat:q7dez5p73vd5jpaizqtyjjhbwy

A Survey on Machine Learning in COVID-19 Diagnosis

Xing Guo, Yu-Dong Zhang, Siyuan Lu, Zhihai Lu
2022 CMES - Computer Modeling in Engineering & Sciences  
Aiming at these problems, we propose some solutions and provide a comprehensive presentation for future research.  ...  For example, the highly unbalanced dataset, the difficulty of collecting labeled data, and the poor quality of the data.  ...  In their experiments, they combined regularization and Mix Match for COVID-19 diagnosis.  ... 
doi:10.32604/cmes.2022.017679 fatcat:hre5zxtekvaevleu335faqilwu

Augmented expansion of Treg cells from healthy and autoimmune subjects via adult progenitor cell co-culture [article]

James L Reading, Valerie Roobrouck, Caroline Hull, Pablo Becker, Jelle Beyens, Alice Valentin-Torres, Dominic A Boardman, Estefania Nova-Lamperti, Samantha Stubblefield, Giovanna Lombardi, Robert Deans, Anthony Ting (+1 others)
2020 bioRxiv   pre-print
Multipotent adult progenitor cells (MAPCR) promote regulatory T cell differentiation in vitro, suggesting they may be repurposed to enhance ex vivo expansion of Tregs for adoptive cellular therapy.  ...  Here, we use a GMP compatible Treg expansion platform to demonstrate that MAPC cell-co-cultured Tregs (MulTreg) exhibit a log- fold increase in yield across two independent cohorts, reducing time to target  ...  Ricky Chan for his assistance with the bioinformatic analysis and Liesbeth Vanenpoel and Ellen Van Houtven for technical assistance.  ... 
doi:10.1101/2020.12.03.410316 fatcat:k7uobptnxvcoxfjwilgq6dxb7y

Severe COVID-19 is marked by a dysregulated myeloid cell compartment

Jonas Schulte-Schrepping, Nico Reusch, Daniela Paclik, Kevin Baßler, Stephan Schlickeiser, Bowen Zhang, Benjamin Krämer, Tobias Krammer, Sophia Brumhard, Lorenzo Bonaguro, Elena De Domenico, Daniel Wendisch (+59 others)
2020 Cell  
Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure.  ...  In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition  ...  After stimulation, cell-free supernatants were collected and tested for IL-1b, IFNg, and TNFa, respectively, using the cytokine bead assay Legend-Plex Mix&Match inflammation panel 1 kit (Biolegend, USA  ... 
doi:10.1016/j.cell.2020.08.001 pmid:32810438 pmcid:PMC7405822 fatcat:z56x2ggsyzdnhgwiipssa2djhe

The Role of Virulence Proteins in Protection Conferred by Bordetella pertussis Outer Membrane Vesicle Vaccines

René H. M. Raeven, Naomi van Vlies, Merijn L. M. Salverda, Larissa van der Maas, Joost P. Uittenbogaard, Tim H. E. Bindels, Jolanda Rigters, Lisa M. Verhagen, Sabine Kruijer, Elly van Riet, Bernard Metz, Arno A. J. van der Ark
2020 Vaccines  
We conclude that the presence of multiple virOMPs in omvPV is crucial for protection against Bordetella pertussis.  ...  This protective immunity does not depend on individual proteins, as their absence or low abundance can be compensated for by other virOMPs.  ...  The concentration of T-helper subset cytokines interleukin-4 (IL-4), IL-5, IL-10, IL-13, IL-17A, TNFα, and IFNγ was determined in the supernatants using a ProcartaPlex Mix&Match Mouse 7-Plex (ThermoFisher  ... 
doi:10.3390/vaccines8030429 pmid:32751680 fatcat:iylxoa3bcfduvhgmihgkdgdxom

Global Shapes of F-actin Depolymerization-competent Minimal Gelsolins

Nagesh Peddada, Amin Sagar, Yogendra S. Rathore, Vikas Choudhary, U. Bharat K. Pattnaik, Neeraj Khatri, Renu Garg, Ashish
2013 Journal of Biological Chemistry  
Because of its ability to rapidly depolymerize F-actin, plasma gelsolin has emerged as a therapeutic molecule in different disease conditions.  ...  We demonstrate that the mobility of the G1 domain, essential for F-actin depolymerization, is indirectly regulated by the gelsolin-like sequence of g2-g3 linker.  ...  Matched buffers (the S200 elution buffer) for each sample were prepared in a similar way.  ... 
doi:10.1074/jbc.m113.463224 pmid:23940055 pmcid:PMC3784735 fatcat:ksakh6erzjgxbk6s4erdlsv6re
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