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Bacteria were centrifuged at 10,000 rpm, 4°C for 10 min, lysed using sonication, and then clarified at 14,000 rpm for 30 min. ...doi:10.1101/254383 fatcat:ftpj3caefrf5pasapkiae4yjiu
Gout is regarded as an autoinflammatory disease induced by the deposition of monosodium urate crystals in the synovial fluid and periarticular tissues. It is characterized by joint symptoms and uric acid nephropathy, as well as other organs involved due to inflammation. Here, we presented a rare case of gout with hearing loss. A 26-year-old Chinese man presented to the Otolaryngology Department with a 1-year history of bilateral ears hearing loss. He had no history of ear injury, fever, rash,doi:10.2478/rir-2021-0018 fatcat:psodv27jkbfnxjfe2afule67qq
more »... adache, nasitis, red eyes, hypertension, and diabetes, and denied exposure to loud noise or ototoxic drugs. Otoscopic examination showed that eardrums were normal. Enhanced magnetic resonance imaging (MRI) scanning detected no abnormalities of inner acoustic meatus or intracranial space-occupying lesion. Auditory brainstem responses (ABR) indicated that the right threshold was increased to 50, and the latency of left V wave and left I-V waves interval were slightly prolonged. Pure tone audiometry (PTA) demonstrated elevated thresholds of 35 dB at 8 kHz in the left ear, while 75 dB and 55 dB at 4 kHz and 8 kHz in the right ear, respectively. Testing for distortion product otoacoustic emissions (DPOAE) found abnormal otoacoustic emissions in the 6-8 kHz frequency of the left ear, and in the 4 kHz and 6-8 kHz frequency of the right ear. He was diagnosed with nerve deafness by the otolaryngologist. Meanwhile, we learned that he had a 3-year history of gout. The right first metatarsophalangeal joint was hurt once; the symptom alleviated voluntarily. Annual follow up showed that serum uric acid increased but without any treatment. Physical examination showed no joint swelling and tenderness, and no ectopic tophus. Laboratory findings showed an increased serum uric acid of 730 mmol/L. Complete blood count, liver and kidney function, C-reactive protein, and erythrocyte sedimentation rate were normal, while anti-nuclear antibodies and antineutrophil cytoplasmic antibodies were negative, and renal ultrasound showed no abnormality. Based on the clinical manifestations and laboratory results, he was diagnosed as gout complicated with nerve deafness. After 3 months of allopurinol monotherapy (100 mg, thrice a day), he had no arthritis and the serum uric acid level was decreased to 362 mmol/L. Intriguingly, his symptom of hearing loss was obviously relieved. Meanwhile, the results of ABR followup was improved with normalization of waves, but PTA and DPOAE results showed no remarkable changes.
These cells on the glass coverslips within the 24-well plate were incubated with 10mg·L -1 proteinase K for 15 min at room temperature. ...doi:10.3748/wjg.v8.i2.357 pmid:11925625 pmcid:PMC4658384 fatcat:opymobdj25hsfbfkjwgu774itm
(A) Before irradiation; (B) after irradiation at 193 nm for 30 min; (C) further irradiation at 248 nm for 2 min; (D) another sample after irradiation at 248 nm for 40 min. ... IR absorption spectra of matrix samples after irradiation at 193 nm for 30 min. ...doi:10.1063/1.471335 fatcat:fezpu6djdrbh7obn7olqpcpiue
Shen and A. Sali, unpubl.) , side chain modeling (B. Webb and A. Sali, unpubl.) , refinement of whole models (B. Webb and A. ... To maintain the 1:1 hydrophobic-to-polar residue ratio found in these proteins, a substantial fraction of hydrophobic residues must be exposed (Shen et al. 2005 ). ...doi:10.1110/ps.062416606 pmid:17075131 pmcid:PMC2242414 fatcat:5ks2ivlxingndjlqsck5w3g6pm
.; Richmond, British Columbia, Canada) antibody diluted 1:1000 with 1% BSA and waited for 30 min. ... Substrate solution was added, and the colorimetric results were photographed using a smartphone after 2 min. ...doi:10.3390/mi12020194 pmid:33668560 fatcat:z4o3vtp6pnafxodm57dijpjibm
2007 Asia-Pacific Microwave Conference
This paper proposes a four-pole quasi-elliptic waveguide filter using multilayer low-temperature cofired ceramic (LTCC) technology. The cavities of the filter can be stacked vertically, while a pair of through hole vias are used to realize the cross coupling path for the quasi-elliptic response. Thus, the circuit size can be reduced by the overlap of the cavity resonators. The proposed filter has 20% size reduction in comparison with a conventional four-pole quasi-elliptic waveguide filter.doi:10.1109/apmc.2007.4554867 fatcat:f6ylmaiw4zdihnx3zhmspull6e
Liu, Yin-Yin Shi, Yan Shen and Jin Sha enrolled the participants, reviewed the information of participants and performed the intelligence tests, Dan Zhang prepared the figures, Yi-Min Zhu, Jian-Zhong ... Author Contributions Gu-Feng Xu and Cheng-Liang Zhou analyzed the data and wrote this manuscript, Yi-Meng Xiong, Jing-Yi Li, Tian-Tian Yu, Shen Tian, Xian-Hua Lin, Yun Liao, Yuan Lv, Fang-Hong Zhang, Zhi-Wei ...doi:10.1016/j.ebiom.2017.05.020 pmid:28532740 pmcid:PMC5478241 fatcat:ksqtqnxmpzf65ishr4k6rid4kq
On average, one can save 20 min for one attraction, and if using the fastpass for six recreational facilities, about 2 h of wait times can be saved. ... ) No lucky drawing mechanism (maintain the current status) (2) Lucky drawing mechanism in place Wait times Currently, in Taiwan's theme parks, the average wait times for one attraction is about 15-20 min ...doi:10.3390/su13137146 fatcat:7kie47xkwbgslml5veep4xufja
A certain amount of this powder (3 mg) was dispersed in 30 mL deionized water (five samples), followed by sonication for 90 min and subsequent centrifugation for 45 min at 64, 256, 576, 1024, and 1600g ... Experimental and Characterization 100 mg graphite powder (≤300 meshes, Alfa Aesar) was dispersed in 200 mL DMF and then sonicated for 60 min in a low-power ultrasonic bath (45 W, 59 Hz). ...doi:10.1039/c3cc46457a pmid:24135994 fatcat:lp4oi2zjy5hxjm5nbrpcmjlls4
Methods/design: To determine whether the traditional Chinese medicine Yi Shen Jian Gu granules could effectively manage AIMSS we will conduct a multicenter, randomized, double-blind, placebo-controlled ... AIMSS, aromatase inhibitor-associated musculoskeletal symptoms; TCM, traditional Chinese medicine; YSJG, Yi Shen Jian Gu granules. ... Shen Jian Gu granules. ...doi:10.1186/1745-6215-15-171 pmid:24885324 pmcid:PMC4030312 fatcat:vx7cirhz3fckldpgytf4fjolze
Supplementary Figure 1 : Graphical illustration of the rotate-and-mirror method for constructing a 2D level-3 hierarchical structure. Square rigid unit or assembly from even levels is rotated counter-clockwise firstly and then mirrored (about M planes) to create assembly for constructing the next level hierarchy. Assembly from odd levels is rotated clockwise and mirrored again to create higher level hierarchies.doi:10.1038/srep41183 pmid:28117427 pmcid:PMC5259711 fatcat:3rwochsplbgy7dui3chgsotsge
A fluid-based method is demonstrated for preparing graphene nanomeshes (GNMs) directly from pristine graphite flakes by a one-step process. The high efficiency is attributed to the combination of fluid-assisted exfoliation and perforation of the graphene sheets. Atomic force microscopy shows that the as-produced GNMs are less than 1.5 nm thick. The total area of the pores within 1 um2 of the GNM sheet is estimated as ~0.15 um2 and the pore density as ~22 um-2, The yield of GNMs from pristinearXiv:1611.00180v1 fatcat:ygg7222vujfv3jrbprebnpk3na
more »... phite powder and the power consumption for per gram GNM synthesis are evaluated as 5 wt% and 120 kWh, respectively. X-ray photoelectron spectroscopy, infrared spectroscopy, elemental analysis and Raman spectroscopy results indicate the purity of the GNMs and thus it is a green efficient method. The present work is expected to facilitate the production of GNMs in large scale.
and 10 min). ... Subsequently, paraffin-embedded tissue sections (4-µm) were dewaxed with 100% xylene at room temperature for 30 min and gradient ethanol solution (100% for 10 min; 100% for 10 min; 95% for 10 min; 80% ...doi:10.3892/or.2019.7067 pmid:30896884 pmcid:PMC6448098 fatcat:qwdnvmu6jfambb5jden36fh2vm
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during pregnancy could result in adverse perinatal outcome. Clinical data on the assessment of the immune response in vaccinated pregnant women and subsequent transplacental antibody transfer are quite limited. Objective: To assess maternal and neonatal neutralizing antibody levels against both wildtype and Delta (B.1.617.2) variants after maternal mRNA vaccination. Study Design: This cohort study was conducted 29 pregnantdoi:10.3390/vaccines10010101 pmid:35062762 pmcid:PMC8778956 fatcat:krhr27uafzdingt3lsihvwhq5a
more »... men who were vaccinated at least one dose of Moderna (mRNA-1273) vaccine. Both neutralizing antibody (wildtype and Delta variant) and S1 receptor binding domain IgG antibody levels were evaluated in maternal and cord blood on the day of delivery. Results: Superiority of antibody level was significant in fully vaccinated women compared with the one-dose group (maternal sera, median, 97.46%; cord sera, median, 97.37% versus maternal sera, median, 4.01%; cord sera, median, 1.44%). No difference in antibody level was noted in relation to interval of second immunization to delivery in the two-dose group (95.99% in 0–2 weeks, 97.45% in 2–4 weeks, 97.48% in 4–8 weeks, 97.72% in 8–10 weeks). The most pronounced reduction was observed for the Delta variant. The wildtype neutralizing antibody level of full-vaccinated women was not influenced by the pertussis vaccination. Conclusion: The data underscore the importance of full vaccination in pregnancy and support the recommendation of COVID-19 immunization for pregnant women. The lower level of vaccine-induced neutralizing antibodies for the Delta variant indicates insufficient protection for mother and newborn and highlights the need for development of effective vaccine strategies.
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