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A Semiautomatic Segmentation Algorithm for Extracting the Complete Structure of Acini from Synchrotron Micro-CT Images

Luosha Xiao, Toshihiro Sera, Kenichiro Koshiyama, Shigeo Wada
2013 Computational and Mathematical Methods in Medicine  
In this study, we develop a semiautomatic segmentation algorithm for extracting the complete structure of acinus from synchrotron micro-CT images of the closed chest of mouse lungs.  ...  The run time is drastically shortened compared with manual methods. These findings suggest that our method may be useful for taking samples used in the statistical analysis of acinus.  ...  Acknowledgments This work is supported in part by JSPS Grants-in-Aid for Scientific Research (no. 24240073) and the MEXT project, "Creating Hybrid Organs of the Future" at Osaka University.  ... 
doi:10.1155/2013/575086 pmid:23533543 pmcid:PMC3600236 fatcat:47txtzrlc5f2jfsg6b2ruwseea

Three-dimensional imaging and morphometric analysis of alveolar tissue from microfocal X-ray-computed tomography

Horst Detlef Litzlbauer, Christoph Neuhaeuser, Alexander Moell, Susanne Greschus, Andreas Breithecker, Folker Ernst Franke, Wolfgang Kummer, Wigbert Stephan Rau
2006 American Journal of Physiology - Lung cellular and Molecular Physiology  
Furthermore, stereologybased morphometry was compared with morphometric semi-automated micro-CT analysis within the same micro-CT scan.  ...  Semi-automated micro-CT analysis resulted in smaller alveolar surface densities (33.4 Ϯ 1.9 vs. 30.5 Ϯ 1/mm; P Ͻ 0.01) with a correlation (r ϭ 0.70; P ϭ 0.023) and agreement of 2.9 Ϯ 1.4/mm.  ...  ACKNOWLEDGMENTS We thank Gerd Magdowski for expert technical advice in osmium tetroxide staining and critical point drying of the tissue samples.  ... 
doi:10.1152/ajplung.00088.2005 pmid:16679382 fatcat:ft5pembhbfcl7lkiv3vdvemgii

Front Matter: Volume 9417

2015 Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging  
SPIE uses a six-digit CID article numbering system in which: The first four digits correspond to the SPIE volume number.  ...  A unique citation identifier (CID) number is assigned to each article at the time of the first publication.  ...  -98] 9417 2O Three-dimensional segmentation of pulmonary artery volume from thoracic computed tomography imaging [9417-99] 9417 2P Microstructure analysis of the pulmonary acinus using a synchrotron  ... 
doi:10.1117/12.2193783 dblp:conf/mibam/X15 fatcat:wxqfpa4ywnd3leqok5xkxipose

Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography

C. L. Walsh, P. Tafforeau, W. L. Wagner, D. J. Jafree, A. Bellier, C. Werlein, M. P. Kühnel, E. Boller, S. Walker-Samuel, J. L. Robertus, D. A. Long, J. Jacob (+6 others)
2021 Nature Methods  
To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using the European Synchrotron Radiation Facility (ESRF)'s Extremely Brilliant  ...  We demonstrate the potential applications of HiP-CT through quantification and morphometry of glomeruli in an intact human kidney and identification of regional changes in the tissue architecture in a  ...  Bayat (INSERM) for help during the test phase, P. Masson (LADAF) for dissections of donors' bodies, H. Reichert (ESRF) and R. Tori for general support of the project and C. Muzelle, R. Homs, C.  ... 
doi:10.1038/s41592-021-01317-x pmid:34737453 pmcid:PMC8648561 fatcat:7iykbzekvjevdikum54w45kmt4

Relationship between structural changes and hyperpolarized gas magnetic resonance imaging in chronic obstructive pulmonary disease using computational simulations with realistic alveolar geometry

M. Plotkowiak, K. Burrowes, J. Wolber, C. Buckley, R. Davies, F. Gleeson, D. Gavaghan, V. Grau
2009 Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences  
Both the development of accurate models of lung function and their quantitative validation can be significantly enhanced by the use of functional imaging techniques.  ...  In particular, application of 3 He to measure apparent diffusion coefficients has enabled some measure of lung microstructure and airspace size within the lung.  ...  Probably the most realistic model to date was presented by Tsuda et al. (2008) , where finiteelement three-dimensional reconstruction of a rat pulmonary acinus, based on high-resolution synchrotron X-ray  ... 
doi:10.1098/rsta.2009.0023 pmid:19414459 fatcat:7rtplbfilna7nl6y6ji7dk7udi

Advanced imaging in COPD: insights into pulmonary pathophysiology

Stephen Milne, Gregory G King
2014 Journal of Thoracic Disease  
This review gives a comprehensive account of the various advanced imaging modalities used to study COPD, including computed tomography (CT), magnetic resonance imaging (MRI), and the nuclear medicine techniques  ...  Chronic obstructive pulmonary disease (COPD) involves a complex interaction of structural and functional abnormalities. The two have long been studied in isolation.  ...  Synchrotron imaging This form of imaging utilises the properties of particle beams, e.g., electrons, in a particle accelerator.  ... 
doi:10.3978/j.issn.2072-1439.2014.11.30 pmid:25478198 pmcid:PMC4255158 fatcat:yv3kvjej5nbpjgprbvrsdfwlzu

Hyperpolarized gas diffusion MRI for the study of atelectasis and acute respiratory distress syndrome

Maurizio Cereda, Yi Xin, Stephen Kadlecek, Hooman Hamedani, Jennia Rajaei, Justin Clapp, Rahim R. Rizi
2014 NMR in Biomedicine  
This paper reviews the contributions of CT, PET, and conventional MRI to understanding this phenomenon.  ...  A number of lung imaging techniques have made substantial headway in clarifying the mechanisms of atelectasis.  ...  Acknowledgments We would like to acknowledge BLES Biochemicals (London, Ontario, Canada) for donating the surfactant formulation used in our experiments.  ... 
doi:10.1002/nbm.3136 pmid:24920074 pmcid:PMC4232982 fatcat:kwnw65omsbfm3chaqzdht3klkq

Alveolar dynamics during mechanical ventilation in the healthy and injured lung

Jana Grune, Arata Tabuchi, Wolfgang M. Kuebler
2019 Intensive Care Medicine Experimental  
Unfortunately, global lung mechanics provide for a poor surrogate of alveolar dynamics and methods for the in-depth analysis of alveolar dynamics on the level of individual alveoli are sparse and afflicted  ...  for a better appreciation of the sometimes diverging findings and interpretations.  ...  About this supplement This article has been published as part of Intensive Care Medicine Experimental Volume 7 Supplement 1 2019: Proceedings from the Third International Symposium on Acute Pulmonary Injury  ... 
doi:10.1186/s40635-019-0226-5 pmid:31346797 pmcid:PMC6658629 fatcat:lhytrpoz3vcafo2wpqk6qplffu

Effective segmentation of fresh post-mortem murine lung parenchyma in phase contrast X-ray tomographic microscopy images

Ioannis V. Oikonomidis, Tiziana P. Cremona, Goran Lovric, Filippo Arcadu, Marco Stampanoni, Johannes C. Schittny
The proposed procedure yields highquality 3D segmentations of acinar microstructure that can be used for a reliable morphological analysis.  ...  As a basis for further studies of pulmonary acini we imaged rodent lungs as close to life as possible using phase contrast synchrotron radiation-based X-ray tomographic microscopy (SRXTM), and developed  ...  Schlepütz for his support at the TOMCAT beamline and for carefully reading the manuscript. We are thankful for the grants 310030-153468 and CR23I2-135550 of the Swiss National Science Foundation.  ... 
doi:10.3929/ethz-b-000192176 fatcat:icy4mm7yhvgs3iwpqewmpfknsq