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Microfluidic device for super-fast evaluation of membrane protein crystallization

Hsin-Jui Wu, Tamara Basta, Mary Morphew, D. C. Rees, Michael H. B. Stowell, Y. C. Lee
2013 The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems  
Here we demonstrate a new concept with a highthroughput membraneless microfluidic device to fast produce the reconstitution of membrane protein nanoparticles.  ...  This virus-like nanoparticle formed by a selfassembly crystallization process of membrane protein and lipids is critical to pharmaceutical industrial.  ...  Pasadena for helpful supports and comments.  ... 
doi:10.1109/nems.2013.6559687 dblp:conf/nems/WuBMRSL13 fatcat:xtjfwyv7o5dldhygcwueo4quiy

Scalable integration of nano-, and microfluidics with hybrid two-photon lithography

Oliver Vanderpoorten, Quentin Peter, Pavan K. Challa, Ulrich F. Keyser, Jeremy Baumberg, Clemens F. Kaminski, Tuomas P. J. Knowles
2019 Microsystems & Nanoengineering  
nanofluidic device fabrication and enhancement of existing microfluidic device architectures with nanofluidic features.  ...  The functionality of the devices was demonstrated through super-resolution microscopy, allowing the observation of features below the diffraction limit of light produced using our approach.  ...  Conflict of interest The authors declare that they have no conflict of interest. Supplementary information accompanies this paper at 10.1038/s41378-019-0080-3.  ... 
doi:10.1038/s41378-019-0080-3 pmid:31636930 pmcid:PMC6799807 fatcat:k22oslf4tfb6ra5q5jaoveb54a

Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics

Samar Damiati, Rami Mhanna, Rimantas Kodzius, Eva-Kathrin Ehmoser
2018 Genes  
Although several challenges remain, the manipulation of biological synthetic machinery in microfluidic devices as suitable 'homes' for in vitro protein synthesis has been proposed as a pioneering approach  ...  This review highlights the recent evolution of cell-free gene design, proteins, and cells integrated with microfluidic platforms as a promising technology, which has allowed for the transformation of the  ...  The formation of the artificial membrane and reconstitution of the VDAC protein was monitored by quartz crystal microbalance with dissipation monitoring (QCM-D) and electrochemical impedance spectroscopy  ... 
doi:10.3390/genes9030144 pmid:29509709 pmcid:PMC5867865 fatcat:irrk7pdg25e47ief2q5o7g4i5i

Systematic Investigation of Insulin Fibrillation on a Chip

Hoon Suk Rho, Henk-Willem Veltkamp, Alexander Thomas Hanke, Marcel Ottens, Christian Breukers, Pamela Habibović, Han Gardeniers
2020 Molecules  
The microfluidic device consists of 64 incubation chambers to perform individual incubations of the protein at 64 specific conditions.  ...  A microfluidic protein aggregation device (microPAD) that allows the user to perform a series of protein incubations with various concentrations of two reagents is demonstrated.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/molecules25061380 pmid:32197443 fatcat:7sowshj7bndynhpzsiixqfuy2y

Label-Free Quantification of Small-Molecule Binding to Membrane Proteins on Single Cells by Tracking Nanometer-Scale Cellular Membrane Deformation

Fenni Zhang, Wenwen Jing, Ashley Hunt, Hui Yu, Yunze Yang, Shaopeng Wang, Hong-Yuan Chen, Nongjian Tao
2018 ACS Nano  
Here we show that the binding of both large and small molecules to membrane proteins can be quantified on single cells by trapping single cells with a microfluidic device, and detecting binding-induced  ...  to determine how strong and fast the binding is 3,6 and evaluate drug potency and efficacy. [6] [7] [8] Zhang et al.  ...  Device from Millipore) PDMS-microfluidic device to trap single cells.  ... 
doi:10.1021/acsnano.8b00235 pmid:29397682 pmcid:PMC5851003 fatcat:niwvnnhwdfa55a6tgerj3vgjia

Telemedicine for insulin detection based on microfluidic system

Ping Yao, Bin Liu, Zaili Dong, Steve Tung
2012 2012 IEEE International Conference on Cyber Technology in Automation, Control, and Intelligent Systems (CYBER)  
This paper reports a microfluidic-based telemedicine system for the determination of insulin, an important indicator for clinical diagnosis of diabetes.  ...  The combination of PDMS microfluidic devices and a communication strategy offers new opportunities for diabetes care and screening, especially in rural areas, and the proposed system can be an extremely  ...  medical evaluation.  ... 
doi:10.1109/cyber.2012.6392541 fatcat:pvfatajfejcn5nrkux7u3s4nw4

Review of MEMS differential scanning calorimetry for biomolecular study

Shifeng Yu, Shuyu Wang, Ming Lu, Lei Zuo
2017 Frontiers of Mechanical Engineering  
relationship in biomolecules such as the folding/unfolding of protein and DNA, and ligand bindings.  ...  Differential scanning calorimetry (DSC) is one of the few techniques that allow direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules.  ...  Fernando Camino for the discussion.  ... 
doi:10.1007/s11465-017-0451-0 fatcat:lwbs3vkssnglnac4akzziw6rqq

Finding the Perfect Match between Nanoparticles and Microfluidics to respond to the Cancer Challenges

F. Raquel Maia, Rui L. Reis, Joaquim M. Oliveira
2019 Nanomedicine: Nanotechnology, Biology and Medicine  
Herein, we review the use of nanoparticles and microfluidic devices to address different cancer challenges, such as detection of CTCs and biomarkers, point-of-care devices for early diagnosis and improvement  ...  On the other hand, microfluidic devices allow the exhibition of physiologically complex systems, incorporation of controlled flow, and control of the chemical environment.  ...  In this reasoning, microfluidic devices and nanoparticles have been strategic for the improvement and evaluation of newly designed pharmacotherapies in vitro.  ... 
doi:10.1016/j.nano.2019.102139 pmid:31843662 fatcat:4u2mcbizyzdzbhfntnp5vst5im

Yeast Nanobiotechnology

Ronnie Willaert, Sandor Kasas, Bart Devreese, Giovanni Dietler
2016 Fermentation  
Nanoscale imaging techniques, such as Atomic Force Microscopy (AFM), super-resolution fluorescence microscopy, and electron microscopy (scanning electron microscopy (SEM), transmission electron microscopy  ...  Other nano-analysis methods include single-molecule and single-cell force spectroscopy and the AFM-cantilever-based nanomotion analysis of living cells.  ...  Conflicts of Interest: The authors declare no conflict of interest.  ... 
doi:10.3390/fermentation2040018 fatcat:bwgcrcwljjfjjghz7xjztzrciq

Breaking the classics: Next-generation biosensors for the isolation, profiling and detection of extracellular vesicles

Raquel Vaz, Verónica M. Serrano, Yuselis Castaño-Guerrero, Ana R. Cardoso, Manuela F. Frasco, M. Goreti F. Sales
2022 Biosensors and Bioelectronics: X  
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version  ...  of record.  ...  For example, a work demonstrated the combination of SPR imaging with antibody microarrays specific for membrane proteins of EVs from tumour cell culture medium (Zhu et al. 2014) .  ... 
doi:10.1016/j.biosx.2022.100115 fatcat:xwlytuci4zevzjndugbas32vgm

Super‐resolution imaging of highly curved membrane structures in giant vesicles encapsulating molecular condensates

Ziliang Zhao, Debjit Roy, Jan Steinkühler, Tom Robinson, Reinhard Lipowsky, Rumiana Dimova
2021 Advanced Materials  
Here, we studied them with super-resolution stimulated emission depletion (STED) microscopy facilitated by immobilization in a microfluidic device.  ...  with molecular crowding and protein condensation.  ...  Z.Z. was supported by Grant SarsRapid by the Bank of Thuringia, Germany during the revision state. Z.Z. acknowledges R. Knorr for assistance when initiating this work.  ... 
doi:10.1002/adma.202106633 pmid:34710248 fatcat:5hhmxans2rbhreabue4l3tbqdm

Precise and Dynamic Temperature Control in High-Resolution Microscopy with VAHEAT

Jaroslav Icha, Daniel Böning, Pierre Türschmann
2022 Microscopy Today  
In this paper, we shed light on these phenomena and introduce our newly developed device VAHEAT, which follows a microscopic approach for controlling and measuring the sample temperature.  ...  It is, however, an inherent technological challenge to precisely track and control the temperature of small sample volumes when using high- and super-resolution microscopes.  ...  Acknowledgements We would like to thank the Optical Imaging Center (OICE) of the Friedrich Alexander University Erlangen-Nuremberg (FAU) and the Max Planck Institute for the Science of Light (MPL) in Erlangen  ... 
doi:10.1017/s1551929521001553 fatcat:fxw4rgn4wrfbjdgmqhbrhfb2ua

Super-resolution imaging of highly curved membrane structures in giant vesicles encapsulating molecular condensates [article]

Ziliang Zhao, Debjit Roy, Jan Steinkuehler, Tom Robinson, Reinhard Lipowsky, Rumiana Dimova
2021 bioRxiv   pre-print
Here, we studied these structures with super-resolution microscopy, namely stimulated emission depletion (STED) microscopy, together with microfluidic manipulation.  ...  with molecular crowding and protein condensation.  ...  Similar "kink"-like structures are observed at membranes of protein storage vacuoles in contact with condensates of seed storage proteins 33 .  ... 
doi:10.1101/2021.08.04.455034 fatcat:oxsqjcpjxvdqlnidslozd3tmuq

Selective Growth of Neural Networks on Micro-Patterned Graphene

Sandeep Keshavan, Matteo Lorenzoni, Fernando Brandi, Andrea Giugni, Francesca Cella Zanacchi, Bruno Torre, Silvia Dante
2014 Biophysical Journal  
Specifically, we found that the symmetry of the bilayer membrane may play a role in Pep-1mediated protein translocation.  ...  We anticipate that the DIB method may be useful for a variety of transport-based studies; in particular those which must make use of tiny quantities of purified species. 1058-Pos Board B813 Cobaltabisdicarbollide  ...  Single crystal graphene is the ideal candidate for next generation of electronic devices and of biosensors.  ... 
doi:10.1016/j.bpj.2013.11.1234 fatcat:hjy6y6pqo5fhzjrz2emwljmnfm

Various On-Chip Sensors with Microfluidics for Biological Applications

Hun Lee, Linfeng Xu, Domin Koh, Nikhila Nyayapathi, Kwang Oh
2014 Sensors  
the device.  ...  In this paper, we review recent advances in on-chip sensors integrated with microfluidics for biological applications.  ...  Therefore, the integration of a sensor in a microfluidic device can have great potential in developing total analysis systems for various applications such as the analysis of DNA, cells, and protein.  ... 
doi:10.3390/s140917008 pmid:25222033 pmcid:PMC4208211 fatcat:uijrpqqrrrbgxivxxap6jtjxum
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