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High transition frequencies of dynamic functional connectivity states in the creative brain

Junchao Li, Delong Zhang, Aiying Liang, Bishan Liang, Zengjian Wang, Yuxuan Cai, Mengxia Gao, Zhenni Gao, Song Chang, Bingqing Jiao, Ruiwang Huang, Ming Liu
2017 Scientific Reports  
Gao, Zhenni Gao and Bingqing Jiao collected the behavior data; and all authors reviewed the manuscript.  ...  and Ming Liu conceived the study; Junchao Li and Delong Zhang performed the data analysis and wrote the paper; Aiying Liang, Bishan Liang, Zengjian Wang, Song Chang collected the MRI data; Yuxuan Cai, Mengxia  ... 
doi:10.1038/srep46072 pmid:28383052 pmcid:PMC5382673 fatcat:xqxwo5drk5cvdjfu7fd3lhp76q

Self-Adaptive Configuration of Visualization Pipeline Over Wide-Area Networks

Qishi Wu, Jinzhu Gao, Mengxia Zhu, Nageswara S.V. Rao, Jian Huang, Sitharama Iyengar
2008 IEEE transactions on computers  
Jinzhu Gao received the PhD degree in computer science from Ohio State University in 2004.  ...  Mengxia Zhu received the PhD degree in computer science from Louisiana State University in 2005.  ... 
doi:10.1109/tc.2007.70777 fatcat:nbr4wovllzegpoknznnpia75lm

Mastitis Süresince Meme Epitel Hücrelerinin Fonksiyonel Durumunu Değerlendirmek Amacıyla Sığır α-kazein Proteinine Karşı Monoklonal Antikor Geliştirilmesi
Development of a Monoclonal Antibody Against Bovine α-casein to Evaluate Functional Status of Mammary Epithelial Cells During Mastitis

Wentao MA, Yi WANG, Fei GAO, Mengxia NING, Ahua LIU, Yanyan LI, Yang GAO, Ping LU, Dekun CHEN
2019 Kafkas Universitesi Veteriner Fakultesi Dergisi  
The substrate (0.3% H 2 O 2 , 0.1% TMB in 0.1M citric acid, and MA, WANG, GAO, NING LIU, LI, GAO, LU, CHEN pH=5) (TIANGEN, Beijing, China) was incubated for 18 min at 37°C, and the reaction was stopped  ...  In this study, we found that the content of α-casein of primary MECs did not change significantly under the stimulation of various concentrations of LPS, indicating that MA, WANG, GAO, NING LIU, LI, GAO  ... 
doi:10.9775/kvfd.2018.20897 fatcat:bvwfpr3yuvhtppeqj3ndgsc7ii

Endothelial-specific deletion of Ets-1 attenuates Angiotensin II-induced cardiac fibrosis via suppression of endothelial-to-mesenchymal transition

Lian Xu, Mengxia Fu, Dongrui Chen, Weiqing Han, Michael C. Ostrowski, Paul Grossfeld, Pingjin Gao, Maoqing Ye
2019 BMB Reports  
Cardiac fibrosis is a common feature in chronic hypertension patients with advanced heart failure, and endothelial-tomesenchymal transition (EndMT) is known to promote Angiotensin II (Ang II)-mediated cardiac fibrosis. Previous studies have suggested a potential role for the transcription factor, ETS-1, in Ang II-mediated cardiac remodeling, however the mechanism are not well defined. In this study, we found that mice with endothelial Ets-1 deletion showed reduced cardiac fibrosis and
more » ... y following Ang II infusion. The reduced cardiac fibrosis was accompanied by decreased expression of fibrotic matrix genes, reduced EndMT with decreased Snail, Slug, Twist, and ZEB1 expression, as well as reduced cardiac hypertrophy and expression of hypertrophyassociated genes was observed. In vitro studies using cultured H5V cells further confirmed that ETS-1 knockdown inhibited TGF-1-induced EndMT. This study revealed that deletion of endothelial Ets-1 attenuated Ang II-induced cardiac fibrosis via inhibition of EndMT, indicating an important ETS-1 function in mediating EndMT. Inhibition of ETS-1 could be a potential therapeutic strategy for treatment of heart failure secondary to chronic hypertension. [BMB Reports 2019; 52(10): 595-600] BMB Rep. 2019; 52(10): 595-600 Deletion of Ets-1 attenuates cardiac fibrosis Lian Xu, et al. 596 BMB Reports
doi:10.5483/bmbrep.2019.52.10.206 fatcat:kp7yroqenndy5pty75th3ipjsy

Identification of CDCA2 as a Diagnostic and Prognostic Marker for Hepatocellular Carcinoma

Zhenjun Yu, Yu Zhang, Shuai Shao, Qi Liu, Yuhan Li, Xiaoxiao Du, Kun Zhang, Mengxia Zhang, Haixia Yuan, Qiang Yuan, Tong Liu, Yingtang Gao (+3 others)
2021 Frontiers in Oncology  
ObjectiveHepatocellular carcinoma (HCC) is one of the most common and malignant tumors with an insidious onset, difficult early diagnosis, and limited therapy options, resulting in a poor prognosis. Cell division cycle associated 2 (CDCA2), also known as Repo-Man, plays an important role in regulating mitosis and DNA repair, but the involvement of CDCA2 in HCC remains unclear.MethodsThe differentially expressed genes that were significantly upregulated in multiple RNA sequencing datasets of HCC
more » ... were screened. Receiver operating characteristic (ROC) curve analysis was performed to identify diagnostic markers for HCC. Least absolute shrinkage and selection operator Cox regression analysis was performed to screen the prognosis-related genes. The screening and analyses identified CDCA2 as the target gene in this study. The expression of CDCA2 was analyzed in public databases and clinical specimens, and CDCA2 involvement in HCC was explored by both bioinformatic analysis and in vitro experiments.ResultsThe level of CDCA2 was enhanced in HCC compared with healthy livers. Overexpression of CDCA2 positively correlated with the pathological grade and TNM stage of the diseases. Furthermore, CDCA2 was found to be an independent prognostic predictor. An excellent prognostic model of HCC was successfully constructed with CDCA2 in combination with TNM stage. Bioinformatic analysis revealed that CDCA2 was closely associated with the cell cycle, apoptosis, and p53 signaling pathway. Silencing CDCA2 in Huh7 cells resulted in significant upregulation of p53 and the downstream PUMA and NOXA and a subsequently increased apoptosis. Inhibition of p53 signaling and apoptosis was found after overexpression of CDCA2 in L02 cells. Strikingly, the proliferation of cells was not affected by CDCA2.ConclusionsCDCA2 was a novel diagnostic marker for HCC, and overexpression of this gene reflected poor pathological grade, stage, and clinical prognosis. CDCA2 promoted the pathogenesis of HCC by suppressing the p53-PUMA/NOXA signaling and the subsequent apoptosis.
doi:10.3389/fonc.2021.755814 pmid:34660326 pmcid:PMC8517522 fatcat:iwu7e4x27vdh3dscf4eefuufsq

Naringin Protects Against High Glucose-Induced Human Endothelial Cell Injury Via Antioxidation and CX3CL1 Downregulation

Guilin Li, Yurong Xu, Xuan Sheng, Hua Liu, Jingjing Guo, Jiayue Wang, Qi Zhong, Huaide Jiang, Chaoran Zheng, Mengxia Tan, Shenqiang Rao, Yanling Yu (+4 others)
2017 Cellular Physiology and Biochemistry  
Background/Aims: The induction of endothelial injury by hyperglycemia in diabetes has been widely accepted. Naringin is a bio-flavonoid. Some studies showed that naringin alleviates diabetic complications, but the exact mechanisms by which naringin improves diabetic anomalies are not yet fully understood. The aim of this research was to study the protective effect of naringin on high glucose-induced injury of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured with or
more » ... without high glucose in the absence or presence of naringin for 5 days. The expression of CX3CL1 was determined by quantitative real-time RT-PCR (qPCR) and western blot. The cellular bioenergetic analysis oxygen consumption rate (OCR) was measured with a Seahorse Bioscience XF analyzer. Results: The production of reactive oxygen species (ROS), the expression of CX3CL1 and the level of AKT phosphorylation were increased in HUVECs cultured with high glucose compared with controls. However, naringin rescued these increases in ROS production, CX3CL1 expression and AKT phosphorylation. Nitric oxide (NO) production and OCR were lower in the high glucose group, and naringin restored the changes induced by high glucose. Molecular docking results suggested that Naringin might interact with the CX3CL1 protein. Conclusion: Naringin protects HUVECs from high-glucose-induced damage through its antioxidant properties by downregulating CX3CL1 and by improving mitochondrial function.
doi:10.1159/000480215 pmid:28848146 fatcat:eyadqhyx55fe5jbp2xivactugi

Association of preoperative urethral parameters on magnetic resonance imaging and immediate recovery of continence following Retzius-sparing robot-assisted radical prostatectomy

Youjian Li, Weijian Li, Wenfeng Lu, Mengxia Chen, Jie Gao, Yang Yang, Junlong Zhuang, Xiaogong Li, Hongqian Guo, Xuefeng Qiu
2020 Translational Andrology and Urology  
Studies regarding predictive factors of urinary continence following Retzius-sparing radical prostatectomy (RP) is limited. This study was designed to evaluate association of urethral parameters on preoperative magnetic resonance imaging (MRI) and immediate recovery of urinary continence following Retzius-sparing robot assisted radical prostatectomy (RS-RARP). This retrospective cohort study enrolled 156 patients with clinically localized prostate cancer who underwent MRI before RS-RARP. We
more » ... ured the following structures on preoperative MRI: minimal residual membranous urethral length (mRUL), peri-urethral sphincter complex (PSC) thickness, urethral wall thickness (UWT), the thicknesses of the levator ani muscle (LAM) and obturator internus muscle (OIM). Immediate urinary continence was defined as patients reported freedom from using safety pad within 7 days after removal of urinary catheter. Patients were divided into two groups according the median of each parameter on MRI. We retrospectively analyzed the patients in term of preoperative clinical factors and postoperative urinary continence. A total of 100 patients (64.1%) reported immediate urinary continence after RS-RARP. Immediate urinary continence was significantly more in patients with longer mRUL (≥8.70 mm) than in patients with shorter mRUL (<8.70 mm; P=0.000). On multivariable analysis, longer mRUL was significantly related to immediate urinary continence after RS-RAPA (odds ratio 8.265; P=0.000). PSC, UWT, LAM and OIM were not associated with immediate urinary continence. Our results firstly demonstrated that preoperative mRUL measured on MRI was an independent predictor of immediate urinary continence following RS-RARP. Therefore, preservation of membranous urethra is still the anatomical basis of better urinary outcome after RS-RARP.
doi:10.21037/tau.2019.12.17 pmid:32420156 pmcid:PMC7215013 fatcat:oh7mpyyy2zdilcivhjjqme7ko4

Combination of 68Ga-PSMA PET/CT and multiparameter MRI improves the detection of clinically significant prostate cancer: a lesion by lesion analysis

Mengxia Chen, Qing Zhang, Chengwei Zhang, Xiaozhi Zhao, Giancarlo Marra, Jie Gao, Xiaoyu Lv, Bing Zhang, Yao Fu, Feng Wang, Xuefeng Qiu, Hongqian Guo
2018 Journal of Nuclear Medicine  
Our purpose was to explore whether 68Ga-PSMA PET/CT alone (PET/CT) or in combination with multiparametric MRI (PET/MRI) can improve the detection of clinically significant prostate cancer (PCa). Methods: We retrospectively enrolled 54 patients who underwent both MRI and PET/CT before radical prostatectomy. Regions of interest on MR images, PET/CT images, and pathologic images were marked. A lesion was defined as a region of interest marked on images obtained with any of the 3 modalities. All
more » ... ions were characterized using the prostate imaging reporting and data system (PI-RADS), the molecular imaging PSMA expression score, and the pathologic results and analyzed. Diagnostic performance was analyzed by receiver-operating-characteristic analysis. Specific improvement for lesions with different PI-RADS scores was analyzed using the net reclassification index (NRI). Results: In total, 90 lesions from 54 patients were analyzed, among which 66 lesions represented clinically significant PCa. Receiver-operating-characteristic analysis showed PET/MRI to perform better than MRI in detecting clinically significant PCa (change in area under the curve, 0.06; 95% confidence interval, 0.01-0.12; P < 0.05). With the calculated cutoff, PET/MRI performed significantly better than MRI (NRI, 21.9%; P < 0.01), with an improvement in sensitivity (89% vs. 76%, P < 0.01) at no sacrifice of specificity (96% vs. 88%, P > 0.05). Improvement in diagnosing clinically significant PCa occurred for lesions classified as PI-RADS 3 (NRI, 66.7%; P < 0.01). Conclusion: PET/MRI improves the detection of clinically significant PCa for PI-RADS 3 lesions.
doi:10.2967/jnumed.118.221010 pmid:30552201 pmcid:PMC6604689 fatcat:bysn6hdf6zearmlcdb5t5i5x74

Protective Effects of Oxymatrine on Vascular Endothelial Cells from High-Glucose-Induced Cytotoxicity by Inhibiting the Expression of A2B Receptor

Yun Yi, Yulin Shen, Qin Wu, Jingan Rao, Shu Guan, Shenqiang Rao, Liping Huang, Mengxia Tan, Lingkun He, Lijuan Liu, Guodong Li, Shangdong Liang (+2 others)
2018 Cellular Physiology and Biochemistry  
Background/Aims: Diabetes mellitus (DM) has become an increasingly epidemic metabolic disease. Vascular endothelial cells play a key role in developing the cardiovascular complications of DM. The A 2B receptor is expressed in vascular endothelial cells, and may help regulate the function of endothelial cells. The aim of this study was to investigate the protective effects of oxymatrine (OMT) on human umbilical vein endothelial cells (HUVECs) from high glucoseinduced cytotoxicity. Methods:
more » ... gy modeling and molecular docking analysis were used to detect the binding sites between the adenosine A 2B receptor and OMT. HUVECs were cultured with control (5.5 mM) or elevated glucose (22.2 mM) in the presence or absence of 3 µM OMT or A 2B siRNA for 3 days. The MTS cell viability assay was used to measure the toxicity of high glucose on HUVECs and the protective effect of OMT or A 2B siRNA. The expression of the adenosine A 2B receptor and CCL5 in HUVECs was detected with real-time quantitative PCR (qPCR) and Western blotting methods in each group. Levels of IL-1β and TNF-α were measured using an enzyme-linked immunosorbent assay (ELISA) kit, and the concentration of NO was detected with the nitrate reductase method. Monocyte chemotactic activity in each group was detected using Transwell chambers. Furthermore, the phosphorylation of p38 and ERK1/2 in each group was observed through the Western blotting method. Results: Homology modeling and molecular docking analysis showed that OMT contains well-fitted Yi et al.: Oxymatrine and A 2B Receptor in Glucose-Induced Cytotoxicity to Huvecs binding sites to the A 2B receptor. After chronic culture at high glucose, the rate of cell viability was significantly lower than that of the control group. After co-treatment with OMT or A 2B siRNA, cell viability was significantly increased compared with the high-glucose group. The results from real-time quantitative RT-PCR (qRT-PCR) and Western blotting indicated that high glucose could increase the expression of A 2B receptors in HUVECs, an effect that was inhibited by OMT. In addition, the results revealed that the expression of CCL5, IL-1β and TNF-α was increased in the high-glucose group, and that the NO produced by HUVECs decreased due to hyperglycemia; however, co-culture with OMT or A 2B siRNA abolished these effects. Meanwhile, the chemotaxis activity of monocytes to HUVECs cultured in high-glucose medium was enhanced 2.59-fold compared to the control cells. However, the inflammatory reactions in HUVECs were completely relieved by co-treatment with OMT or A 2B siRNA. Moreover, the phosphorylation of p38 and ERK1/2 in HUVECs in the high-glucose group was significantly higher than that of the control group; these effects were reversed after co-treatment with OMT or A 2B siRNA. Conclusion: OMT may protect the HUVECs from high glucose-induced cytotoxicity through inhibitting the expression of A 2B receptor and inflammatory factors as well as decreasing the phosphorylation of p38 and ERK1/2.
doi:10.1159/000487033 pmid:29402837 fatcat:tce66v7vurfphdysnqjzsiqijm

Consensus on potential biomarkers developed for use in clinical tests for schizophrenia

Ping Lin, Junyu Sun, Xiaoyan Lou, Dan Li, Yun Shi, Zhenhua Li, Peijun Ma, Ping Li, Shuzi Chen, Weifeng Jin, Shuai Liu, Qing Chen (+13 others)
2022 General Psychiatry  
BackgroundSchizophrenia is a serious mental illness affecting approximately 20 million individuals globally. Both genetic and environmental factors contribute to the illness. If left undiagnosed and untreated, schizophrenia results in impaired social function, repeated hospital admissions, reduced quality of life and decreased life expectancy. Clinical diagnosis largely relies on subjective evidence, including self-reported experiences, and reported behavioural abnormalities followed by
more » ... ric evaluation. In addition, psychoses may occur along with other conditions, and the symptoms are often episodic and transient, posing a significant challenge to the precision of diagnosis. Therefore, objective, specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.AimsWe aimed to provide evidence-based and consensus-based recommendations, with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia, and to discuss directions for future research.MethodsWe searched publications within the last 10 years with the following keywords: 'schizophrenia', 'gene', 'inflammation', 'neurotransmitter', 'protein marker', 'gut microbiota', 'pharmacogenomics' and 'biomarker'. A draft of the consensus was discussed and agreed on by all authors at a round table session.ResultsWe summarised the characteristics of candidate diagnostic markers for schizophrenia, including genetic, inflammatory, neurotransmitter, peripheral protein, pharmacogenomic and gut microbiota markers. We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.ConclusionsFurther efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis, differential diagnosis and ethnicity-specific markers for the Chinese population. The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease.
doi:10.1136/gpsych-2021-100685 pmid:35309241 pmcid:PMC8867318 fatcat:khdej36oq5bw7nr5l6itfgimoa

An extended application of β-Ga2O3 single crystals to the laser field: Cr4+:β-Ga2O3 utilized as a new promising saturable absorber

Wenxiang Mu, Yanru Yin, Zhitai Jia, Lijuan Wang, Jie Sun, Mengxia Wang, Cheng Tang, Qiangqiang Hu, Zeliang Gao, Jian Zhang, Na Lin, Stefano Veronesi (+3 others)
2017 RSC Advances  
A Cr4+-doped β-Ga2O3 crystal has been grown successfully and demonstrated as a saturable absorber for the first time.
doi:10.1039/c7ra01905j fatcat:bohvgsgjajh3xm247bogk2gmmi

Edge stabilization in reduced-dimensional perovskites

Li Na Quan, Dongxin Ma, Yongbiao Zhao, Oleksandr Voznyy, Haifeng Yuan, Eva Bladt, Jun Pan, F. Pelayo García de Arquer, Randy Sabatini, Zachary Piontkowski, Abdul-Hamid Emwas, Petar Todorović (+18 others)
2020 Nature Communications  
Reduced-dimensional perovskites are attractive light-emitting materials due to their efficient luminescence, color purity, tunable bandgap, and structural diversity. A major limitation in perovskite light-emitting diodes is their limited operational stability. Here we demonstrate that rapid photodegradation arises from edge-initiated photooxidation, wherein oxidative attack is powered by photogenerated and electrically-injected carriers that diffuse to the nanoplatelet edges and produce
more » ... de. We report an edge-stabilization strategy wherein phosphine oxides passivate unsaturated lead sites during perovskite crystallization. With this approach, we synthesize reduced-dimensional perovskites that exhibit 97 ± 3% photoluminescence quantum yields and stabilities that exceed 300 h upon continuous illumination in an air ambient. We achieve green-emitting devices with a peak external quantum efficiency (EQE) of 14% at 1000 cd m-2; their maximum luminance is 4.5 × 104 cd m-2 (corresponding to an EQE of 5%); and, at 4000 cd m-2, they achieve an operational half-lifetime of 3.5 h.
doi:10.1038/s41467-019-13944-2 pmid:31924790 fatcat:5wmru3ungjbprg4j4o7q654lhi

Comparison of 68Ga-prostate-specific membrane antigen (PSMA) positron emission tomography/computed tomography (PET/CT) and multi-parametric magnetic resonance imaging (MRI) in the evaluation of tumor extension of primary prostate cancer

Mengxia Chen, Qing Zhang, Chengwei Zhang, Yi-Hua Zhou, Xiaozhi Zhao, Yao Fu, Jie Gao, Bing Zhang, Feng Wang, Xuefeng Qiu, Hongqian Guo
2020 Translational Andrology and Urology  
To compare the diagnostic performance of 68Ga-prostate-specific membrane antigen positron emission tomography/computed tomography (68Ga-PSMA PET/CT) with multi-parametric magnetic resonance imaging (mpMRI) on extracapsular extension (ECE) and seminal vesicle invasion (SVI) in primary prostate cancer and its impact on therapeutic decisions. We retrospectively enrolled 54 patients with both PET/CT and mpMRI before radical prostatectomy. Diagnostic performance of mpMRI, PET/CT and their
more » ... (com-MRI/PET) on ECE and SVI on a patient basis were analyzed. The impact of additional PET/CT scanning on therapeutic decisions were presented. Among the 54 patients, 17 had tumor limited in the prostate gland, 25 only had ECE and 12 patients had both SVI and ECE on pathology. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of ECE were 54%, 94%, 95%, 48% on mpMRI, 78%, 94%, 97%, 67% on PET/CT and 83%, 88%, 94%, 71% on com-MRI/PET. Both PET/CT and com-MRI/PET had a higher sensitivity than mpMRI on ECE diagnosis (78% vs. 54%, P<0.05 and 83% vs. 54%, P<0.05). No difference was observed between PET/CT and com-MRI/PET (78% vs. 83%, P=0.17). The Sensitivity, specificity, PPV and NPV of SVI were 67%, 93%, 72%, 91% on mpMRI, 75%, 95%, 82%, 93% on PET/CT and 75%, 88%, 64%, 93% on com-MRI/PET. No difference was found between the three scannings. After the additional evaluation of PET/CT, 18.5% (10/54) turned from nerve-sparing surgery to non-nerve sparing surgery. 68Ga-PSMA PET/CT has a higher sensitivity on ECE detection than mpMRI but shows no superiority on SVI.
doi:10.21037/tau.2020.03.06 pmid:32420143 pmcid:PMC7215027 fatcat:43asi2kabnar7juu5ikyoprz6i

The evolution of ancestral and species-specific adaptations in snowfinches at the Qinghai-Tibet Plateau

Yanhua Qu, Chunhai Chen, Xiumin Chen, Yan Hao, Huishang She, Mengxia Wang, Per G P Ericson, Haiyan Lin, Tianlong Cai, Gang Song, Chenxi Jia, Chunyan Chen (+11 others)
2021 Proceedings of the National Academy of Sciences of the United States of America  
Species in a shared environment tend to evolve similar adaptations under the influence of their phylogenetic context. Using snowfinches, a monophyletic group of passerine birds (Passeridae), we study the relative roles of ancestral and species-specific adaptations to an extreme high-elevation environment, the Qinghai-Tibet Plateau. Our ancestral trait reconstruction shows that the ancestral snowfinch occupied high elevations and had a larger body mass than most nonsnowfinches in Passeridae.
more » ... equently, this phenotypic adaptation diversified in the descendant species. By comparing high-quality genomes from representatives of the three phylogenetic lineages, we find that about 95% of genes under positive selection in the descendant species are different from those in the ancestor. Consistently, the biological functions enriched for these species differ from those of their ancestor to various degrees (semantic similarity values ranging from 0.27 to 0.5), suggesting that the three descendant species have evolved divergently from the initial adaptation in their common ancestor. Using a functional assay to a highly selective gene, DTL, we demonstrate that the nonsynonymous substitutions in the ancestor and descendant species have improved the repair capacity of ultraviolet-induced DNA damage. The repair kinetics of the DTL gene shows a twofold to fourfold variation across the ancestor and the descendants. Collectively, this study reveals an exceptional case of adaptive evolution to high-elevation environments, an evolutionary process with an initial adaptation in the common ancestor followed by adaptive diversification of the descendant species.
doi:10.1073/pnas.2012398118 pmid:33753478 fatcat:ptkqey6s2ffbpieyhckn2dgbim

Synergistic Effect of Co-Delivering Ciprofloxacin and Tetracycline Hydrochloride for Promoted Wound Healing by Utilizing Coaxial PCL/Gelatin Nanofiber Membrane

Mengxia Lin, Yuan Liu, Junwei Gao, Donghui Wang, Dan Xia, Chunyong Liang, Ning Li, Ruodan Xu
Combining multiple drugs or biologically active substances for wound healing could not only resist the formation of multidrug resistant pathogens, but also achieve better therapeutic effects. Herein, the hydrophobic fluoroquinolone antibiotic ciprofloxacin (CIP) and the hydrophilic broad-spectrum antibiotic tetracycline hydrochloride (TH) were introduced into the coaxial polycaprolactone/gelatin (PCL/GEL) nanofiber mat with CIP loaded into the PCL (core layer) and TH loaded into the GEL (shell
more » ... ayer), developing antibacterial wound dressing with the co-delivering of the two antibiotics (PCL-CIP/GEL-TH). The nanostructure, physical properties, drug release, antibacterial property, and in vitro cytotoxicity were investigated accordingly. The results revealed that the CIP shows a long-lasting release of five days, reaching the releasing rate of 80.71%, while the cumulative drug release of TH reached 83.51% with a rapid release behavior of 12 h. The in vitro antibacterial activity demonstrated that the coaxial nanofiber mesh possesses strong antibacterial activity against E. coli and S. aureus. In addition, the coaxial mats showed superior biocompatibility toward human skin fibroblast cells (hSFCs). This study indicates that the developed PCL-CIP/GEL-TH nanofiber membranes hold enormous potential as wound dressing materials.
doi:10.3390/ijms23031895 pmid:35163814 pmcid:PMC8836966 fatcat:2b6yatsq5rcp7emmo7ze3nmsey
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