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Boron mediated 2D and 3D cultures of adipose derived mesenchymal stem cells

Özge Ekin Akdere, İnci Shikhaliyeva, Menemşe Gümüşderelioğlu
2019 Cytotechnology (Dordrecht)  
Surface properties of the scaffolds has a great effect on cell adhesion and spreading (Beşkardeş and Gümüşderelioglu 2009 ).  ... 
doi:10.1007/s10616-019-00310-9 pmid:30905012 pmcid:PMC6465390 fatcat:tzilsmb7srdtrc3f25uu5qox64

Surface properties and exopolysaccharide production of surface-associated microorganisms isolated from a dairy plant

Dilay Kütük Ayhan, Ayhan Temiz, Farzin Asghari Sana, Menemşe Gümüşderelioğlu
2019 Annals of Microbiology  
Purpose The purpose of this study was to isolate the surface-associated microorganisms from the dairy plant surfaces with a high probability of biofilm formation and determine the most adhesive strains in terms of surface properties and exopolysaccharide production. Methods Four hundred and ninety-five surface-associated microorganisms were isolated from potential biofilm-forming surfaces of a dairy plant. One hundred and seventy of these were isolated after cleaning/disinfection of the
more » ... zed milk, white cheese and butter tank, yogurt and ice cream filling unit, ice cream air pressing, and condensed milk pipe. It is noteworthy that some isolates might cause post-production contamination, food infection, and intoxication. Selected 42 isolates were identified by Gram staining, physiological and biochemical tests, and 16S rRNA gene sequencing. Then, surface properties and exopolysaccharide production of 10 selected isolates were determined. To evaluate the surface properties, microbial adhesion to hydrocarbons, static water contact angle, salt aggregation, and surface zeta potential tests were performed. Result The microbial adhesion to hydrocarbons (MATH) test exhibited the lowest standard deviations, and the most consistent results between the replicates. The highest hydrophilic characteristics and exopolysaccharide production were exhibited by Gram-negative Pseudomonas aeruginosa, followed by Gram-positive Bacillus toyonensis. Also, a significant diversity of neutral sugar was determined in their alditol acetate forms by using gas chromatography-mass spectrometry. In this context, it is believed that the determination of the EPS content of the isolates would contribute to establishing an effective cleaning/disinfection procedure for dairy plants. Conclusion This study indicated that microbial adhesion is still a common problem in the dairy industry. Because of this situation, dairy plants should be organized and constructed to be suitable for hygiene and sanitary applications.
doi:10.1007/s13213-019-01482-7 fatcat:2fvm2u25xzawtjcrj6tjpj5y3u

Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering

Zeliha Soran, R. Seda Tığlı Aydın, Menemşe Gümüşderelioğlu
2012 Journal of Microencapsulation  
Due to its biocompatibility, non-toxic features and cheapness, alginate is widely used in different applications (T|gl| and Gümüşderelioglu, 2009) .  ... 
doi:10.3109/02652048.2012.686531 pmid:22612554 fatcat:3t22gfxrpzclbj5zv6dnv6ueoy

Enhanced osteogenic activity with boron-doped nanohydroxyapatite-loaded poly(butylene adipate-co-terephthalate) fibrous 3D matrix

Aysu Arslan, Soner Çakmak, Menemşe Gümüşderelioğlu
2018 Artificial Cells Nanomedicine and Biotechnology  
In this study, three dimensional (3D) poly(butylene adipate-co-terephthalate) (PBAT) fibrous scaffolds with more than 90% porosity were fabricated via wet electrospinning method. Amorphous hydroxyapatite (HAp) and boron (B) doped hydroxyapatite (B-HAp) nanoparticles were produced by microwaveassisted biomimetic precipitation and encapsulated into PBAT fibres with the ratio of 5% (w/w) in order to enhance osteogenic activity of the scaffolds. Cell culture studies were carried out with human bone
more » ... marrow derived stem cells (hBMSCs) and they showed that alkaline phosphatase (ALP) activity and the amounts of collagen and calcium were higher on B containing PBAT (B-HAp-PBAT) scaffolds during the 28-day culture period than that of the PBAT scaffolds. Moreover, hBMSCs cultivated on B-HAp-PBAT scaffolds showed significantly higher expression levels of both early and late stage osteogenic genes e.g. ALP, collagen I (COL-I), osteocalcin (OCN) and osteopontin (OPN) at day 28 than that of the PBAT scaffolds. Scanning electron microscope (SEM) photographs and energy dispersive X-ray (EDX) analysis indicated that hBMSCs produced high amounts of mineralized extracellular matrix (ECM) mainly on the surface of the 3 D matrices. This study demonstrates that boron-containing 3 D nanofibrous PBAT scaffolds with their osteoinductive and osteoconductive properties can be used as alternative constructs for bone tissue engineering. ARTICLE HISTORY
doi:10.1080/21691401.2018.1470522 pmid:29749273 fatcat:wzlbgbb5hnbuvjvpaesxfrdkdm

Comparative chondrogenesis of human cell sources in 3D scaffolds

R. Seda Tıǧlı, Sourabh Ghosh, Michael M. Laha, Nirupama K. Shevde, Laurence Daheron, Jeffrey Gimble, Menemşe Gümüşderelioǧlu, David L. Kaplan
2009 Journal of Tissue Engineering and Regenerative Medicine  
Cartilage tissue can be engineered by starting from a diversity of cell sources, including stem-cell based and primary cell-based platforms. Selecting an appropriate cell source for the process of cartilage tissue engineering or repair is critical and challenging, due to the variety of cell options available. In this study, cellular responses of isolated human chondrocytes, human embryonic stem cells and mesenchymal stem cells (MSCs) derived from three sources, human embryonic stem cells, bone
more » ... arrow and adipose tissue, were assessed for chondrogenic potential in 3D culture. All cell sources were characterized by FACS analysis to compare expression of some surface markers. The cells were differentiated in two different biomaterial matrices, silk and chitosan scaffolds, in the presence and absence of bone morphogenetic protein 6 (BMP6), along with the standard chondrogenic differentiating factors. Embryonic stem cells-derived MSCs showed unique characteristics, with preserved chondrogenic phenotype in both scaffolds with regard to chondrogenesis, as determined by real time RT-PCR, histological and microscopical analyses. After 4 weeks of cultivation, embryonic stem cells-derived MSCs were promising for chondrogenesis, particularly in the silk scaffolds with BMP6. The results suggest that cell source differences are important to consider with regard to chondrogenic outcomes, and among the variables addressed here the human embryonic stem cells-derived MSCs were the preferred cell source.
doi:10.1002/term.169 pmid:19382119 pmcid:PMC2791320 fatcat:frrk25q25jcoxhnlup23xha72a

Effect of melatonin/BMP-2 co-delivery scaffolds on the osteoclast activity

Hala Jarrar, Damla Çetin Altındal, Menemşe Gümüşderelioğlu
2021 Journal of materials science. Materials in medicine  
AbstractBone morphogenetic protein two (BMP-2) has been widely used as an osteoinductive agent in the treatment of bone diseases. However, some side effects, such as osteoclast activation have emerged when it was used at high doses. In this study, by considering the osteoclast-suppressing capability of melatonin (MEL), its effect on osteoclast differentiation induced by BMP-2 was investigated. These two factors, MEL and BMP-2, were embedded into chitosan/hydroxyapatite (HAp) scaffolds that were
more » ... characterized morphologically by scanning electron microscopy (SEM) and micro-computed tomography (μ-CT). Release profiles of MEL and BMP-2 from scaffolds were determined in vitro and then, the differentiation of RAW 264.7 cells to osteoclasts was investigated on the scaffolds. Results of tartrate-resistant acid phosphatase (TRAP) staining, SEM imaging and expression of cathepsin K gene showed that, in the presence of BMP-2, osteoclast differentiation increased, whereas it decreased in MEL and MEL/BMP-2 embedded scaffolds suggesting that melatonin successfully attenuated osteoclast differentiation induced by BMP-2. Thus, the MEL/BMP-2 loaded chitosan/HAp scaffolds that have dual function in enhancing bone formation and inhibiting osteoclast activity are recommended biomaterials in the field of bone regeneration.
doi:10.1007/s10856-021-06502-0 pmid:33751250 pmcid:PMC7983354 fatcat:yyll2vqm6zarha26mkirowrfoi

Osteogenic differentiation of electrostimulated human mesenchymal stem cells seeded on silk-fibroin films

Anıl S. ÇAKMAK, Soner ÇAKMAK, James D. WHITE, Waseem K. RAJA, David L. KAPLAN, Menemşe GÜMÜŞDERELİOĞLU
2016 Turkish Journal of Biology  
Electric field is known as an important regulator to guide the development and regeneration of many tissues. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk-fibroin films in response to different parameters, i.e. frequency, voltage, distance between electrodes, and/or culture conditions (growth medium or osteogenic medium). Silk films were prepared in the presence of platinum wires to study the impact
more » ... exogenous electrostimulation on the cells for up to 14 days. The experimental groups can be defined as high voltage in osteogenic differentiation medium, low voltage in osteogenic differentiation medium, and low voltage in growth medium in this study. Compared to the unstimulated controls (silk films without platinum wires), low voltage (10 mV) did not influence proliferation, while it enhanced osteogenic differentiation according to early and late osteogenic markers in osteogenic differentiation medium. In growth medium, low voltage increased cell proliferation in contrast to osteogenic medium. On the other hand, high voltage (500 mV) stimulated cell proliferation and only late osteogenic markers in osteogenic medium. The results suggest the potential to exploit exogenous biophysical control of cell functions towards tissue-specific goals.
doi:10.3906/biy-1505-114 fatcat:fiovr4azmnakjmurjs4njb6bva

Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin

Farzin Asghari Sana, Merve Çapkın Yurtsever, Gökçe Kaynak Bayrak, Ekin Özge Tunçay, Arlin S. Kiremitçi, Menemşe Gümüşderelioğlu
2017 Cytotechnology (Dordrecht)  
The authors are grateful to Selin Gümüşderelioglu for editing of language in the text.  ... 
doi:10.1007/s10616-017-0072-9 pmid:28653139 pmcid:PMC5507842 fatcat:32qv7wobmnf3tpuukbsz32rrry

A comparative study on monomer elution and cytotoxicity of different adhesive restoration materials

Tuğba Toz, Arlin Kiremitçi, Anıl Sera Çakmak, Oya Ünsal Tan, Erhan Palaska, Menemşe Gümüşderelioğlu, Mutlu Özcan
2016 Journal of Adhesion Science and Technology  
This study evaluated monomer release and cytotoxicity of different adhesive restoration materials used for dental restorations. The extracts (1, 2, and 7 days) of three types of adhesive dental restoration materials, [Quixfill (QF), Silorane Restorative (SR), and Ketac N 100 Restorative (KR)], and the adhesive resins, [XP Bond (XP), Silorane Primer (SP), Ketac N 100 Primer (KP), and Silorane Bond (SB)] were analyzed using high performance liquid chromatography/mass spectrometry (HPLC-MS). The
more » ... totoxicity levels were determined at different time points (24, 48, and 72 h) of cell culture using 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. All adhesive resin materials showed monomer release at varying amounts with the highest release after 7 days. The lowest amount of release was observed in QF and the highest with KP. Bis-Phenol A (BPA) was not detected in SP and KR that contain bisphenol-A diglycidyl ether dimethacrylate (bis-GMA). Decamethylpenthasiloxane (D5) was not eluted from SR. Except for SR and QF, all other adhesive restoration materials showed different degrees of toxicity along with different monomer release kinetics. The correlation between the monomer release and cytotoxicity of the materials indicated that the cytotoxicity of the materials increased with the monomer release (Spearman's rho correlation coefficient -r). The correlation after 48 h was statistically significant (r = −0.342, p = 0.017).
doi:10.1080/01694243.2016.1215768 fatcat:ebwtzxb22zhv7kyhig47keoj4i

Synergistic effect of exogeneous and endogeneous electrostimulation on osteogenic differentiation of human mesenchymal stem cells seeded on silk scaffolds

Anıl S. Çakmak, Soner Çakmak, James D. White, Waseem K. Raja, Kyungsook Kim, Sezin Yiğit, David L. Kaplan, Menemşe Gümüşderelioğlu
2015 Journal of Orthopaedic Research  
Bioelectrical regulation of bone fracture healing is important for many cellular events such as proliferation, migration, and differentiation. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk scaffolds in response to different modes of electrostimulation (e.g., exogeneous and/or endogeneous). Endogeneous electrophysiology was altered through the use of monensin (10 nM) and glibenclamide (10 mM), along
more » ... h external electrostimulation (60 kHz; 100-500 mV). Monensin enhanced the expression of early osteogenic markers such as alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX-2). When exogeneous electrostimulation was combined with glibenclamide, more mature osteogenic marker upregulation based on bone sialoprotein expression (BSP) and mineralization was found. These results suggest the potential to exploit both exogeneous and endogeneous biophysical control of cell functions towards tissue-specific goals.
doi:10.1002/jor.23059 pmid:26419698 fatcat:hwqn76fpsbc3babkxqfdomd5ty

A comparative study on monomer elution and cytotoxicity of different adhesive restoration materials

Tuğba Toz, Arlin Kiremitçi, Anıl Sera Çakmak, Oya Ünsal Tan, Erhan Palaska, Menemşe Gümüşderelioğlu, Mutlu Özcan
2017
This study evaluated monomer release and cytotoxicity of different adhesive restoration materials used for dental restorations. The extracts (1, 2, and 7 days) of three types of adhesive dental restoration materials, [Quixfill (QF), Silorane Restorative (SR), and Ketac N 100 Restorative (KR)], and the adhesive resins, [XP Bond (XP), Silorane Primer (SP), Ketac N 100 Primer (KP), and Silorane Bond (SB)] were analyzed using high performance liquid chromatography/mass spectrometry (HPLC-MS). The
more » ... totoxicity levels were determined at different time points (24, 48, and 72 h) of cell culture using 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. All adhesive resin materials showed monomer release at varying amounts with the highest release after 7 days. The lowest amount of release was observed in QF and the highest with KP. Bis-Phenol A (BPA) was not detected in SP and KR that contain bisphenol-A diglycidyl ether dimethacrylate (bis-GMA). Decamethylpenthasiloxane (D5) was not eluted from SR. Except for SR and QF, all other adhesive restoration materials showed different degrees of toxicity along with different monomer release kinetics. The correlation between the monomer release and cytotoxicity of the materials indicated that the cytotoxicity of the materials increased with the monomer release (Spearman's rho correlation coefficient -r). The correlation after 48 h was statistically significant (r = −0.342, p = 0.017).
doi:10.5167/uzh-146611 fatcat:utq2mlcr2rga7kxasw2twq7vui

Evaluation of semi-interpenetrating polymer networks composed of chitosan and poloxamer for wound dressing application

I.Y. Kim, M.K. Yoo, J.H. Seo, S.S. Park, H.S. Na, H.C. Lee, S.K. Kim, C.S. Cho
2007 International Journal of Pharmaceutics  
Tolga Demirtaş, Menemşe Gümüşderelioğlu P072 Nanostructure of Chitosan/DNA Nanoparticles Triggered by Added Salt and Hydrophobic P073 In Situ Synthesis of Superparamagnetic Nanoparticles in Presence of  ... 
doi:10.1016/j.ijpharm.2007.03.042 pmid:17482781 fatcat:w4im67rzznfwzfhkxevaeqlwpm

VIABILITY AND MORPHOLOGY OF HUMAN DENTAL PULP STEM CELLS IN THE PRESENCE OF CITRUS PECTIN

Gamze AYAR, Merve ÇAPKIN YURTSEVER
2020 Natural and Applied Sciences Journal  
Menemşe Gümüşderelioğlu, from Hacettepe University, Turkey.  ... 
doi:10.38061/idunas.745983 fatcat:3yi6kd6mkrepziz2tltbd2scxe

Turkish Female Academicians and their Contributions to Sciences

Günseli Naymansoy
2010 Gaziantep University Journal of Social Sciences  
Genç kuşak temel bilimcilerden Ayşe Erzan, 2003 yılında "L'Oreal-UNESCO Uluslararası Bilim ve Kadın Ödülü"nü, Menemşe Kiremitçi Gümüşderelioğlu, 1988 yılında "Avrupa Hayvan Hücre Teknolojisi Genç Bilim  ... 
doaj:6c9b5cc228ad43d6ae3c10977a606ba9 fatcat:b2si6hbe6nbjncpam2ynif3toe

Διαφοροποίηση μονοκυττάρων προς ενδοθηλιακά κύτταρα: καλλιέργεια σε σταθερά υποστρώματα

Ηρώ Γ. Κολιάκου
2018
., Menemşe Gumusderelioglu, Aylin Gürpinar, and Mehmet Ali Onur. 2008. ‘Comparison of Cellular Proliferation on Dense and Porous PCL Scaffolds’.  ... 
doi:10.26262/heal.auth.ir.300535 fatcat:kvrywgrzobbrrbgpyupq4mo46e