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Confidential: patent pending measuring blood cells velocity in microvessels from a single image: application to in vivo and in situ confocal microscopy

N. Savoire, G. Le Goualher, A. Perchant, F. Lacombe, G. Malandain, N. Ayache
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano (IEEE Cat No. 04EX821)  
This article describes an original method to measure the velocity of blood cells in microvessels from a single image.  ...  Preliminary experiments show good quantitative results in synthetic images of microvessels, and good qualitative results with real images of microvessels acquired in vivo and in situ at a temporal frequency  ...  INTRODUCTION Velocity measurements of blood cells in microvessels are usually obtained from the processing of 2D temporal image sequences obtained in the field of intravital microscopy.  ... 
doi:10.1109/isbi.2004.1398573 fatcat:y2ufvnpbfvdcbmoxe6xtpk675e


Cornelia Halin, J. Rodrigo Mora, Cenk Sumen, Ulrich H. von Andrian
2005 Annual Review of Cell and Developmental Biology  
Recent advances in microscopy now make it possible to image cell migration and cell-cell interactions in vivo deep within intact tissues.  ...  Over the past decades, intravital microscopy (IVM), the imaging of cells in living organisms, has become a valuable tool for studying the molecular determinants of lymphocyte trafficking.  ...  ) Q = v blood * π * D v 2 /4 * 10 −6 nl/s Critical velocity (v crit ) The lowest velocity that a non-interacting cell can assume in a microvessel.  ... 
doi:10.1146/annurev.cellbio.21.122303.133159 pmid:16212508 fatcat:awvwnylsk5flhgshxblazeghfe

Intestinal ischemia-reperfusion injury: reversible and irreversible damage imaged in vivo

Yanfang Guan, Roger T. Worrell, Timothy A. Pritts, Marshall H. Montrose
2009 American Journal of Physiology - Gastrointestinal and Liver Physiology  
IR was imposed by inflation/deflation of a vascular occluder, and blood flow was monitored and confirmed with scanning confocal imaging.  ...  Intestinal ischemia-reperfusion injury: reversible and irreversible damage imaged in vivo. The early events in an intestinal ischemic episode have been difficult to evaluate.  ...  With the use of confocal microscopy, RBC speed in single capillaries (diameter 3-6 m) was measured to assess semiquantitatively the rate of blood flow.  ... 
doi:10.1152/ajpgi.90595.2008 pmid:19407214 pmcid:PMC2889629 fatcat:dws2xmjmkvbu3hbt5o4pep67my

In vivo flow cytometry: A horizon of opportunities

Valery V. Tuchin, Attila Tárnok, Vladimir P. Zharov
2011 Cytometry Part A  
Invasive extraction of cells from a living organism, however, may lead to changes in cell properties and prevents studying cells in their native environment.  ...  These problems can be overcome by use of in vivo flow cytometry which provides detection and imaging of circulating normal and abnormal cells directlyin blood or lymph flow.  ...  (in vivo image FCM) of flowing single cells of interest at velocities up to 2 m/s (23, 34, 40) ; 3) PT and PA measurements of the velocity of individual flowing cells (19, 29, 42) and of cell flows  ... 
doi:10.1002/cyto.a.21143 pmid:21915991 pmcid:PMC3663136 fatcat:j5zbeb2lkrcw5ojj2b4vomb6zy

Live Imaging of the Lung

Mark R. Looney, Jahar Bhattacharya
2014 Annual Review of Physiology  
Live lung imaging has spanned the discovery of capillaries in the frog lung by Malpighi to the current use of single and multiphoton imaging of intravital and isolated perfused lung preparations incorporating  ...  New applications of live lung imaging and the limitations of current techniques are discussed.  ...  The work was supported by NHLBI grants HL107386 (to M.R.L.) and HL36024, HL57556, and HL78645 (to J.B.). LITERATURE CITED  ... 
doi:10.1146/annurev-physiol-021113-170331 pmid:24245941 pmcid:PMC4501380 fatcat:j4l63nhjyranbl7ioawx4r56he

Imaging in the era of molecular oncology

Ralph Weissleder, Mikael J. Pittet
2008 Nature  
in vivo and how their location changes over time.  ...  Advances in experimental and clinical imaging are likely to improve how cancer is understood at a systems level and, ultimately, should enable doctors not only to locate tumours but also to assess the  ...  Acknowledgements The authors acknowledge financial support from the National Institutes of Health.  ... 
doi:10.1038/nature06917 pmid:18385732 pmcid:PMC2708079 fatcat:vqdqb6d37bhw3omg3qqoxc35du

Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry

Lior Golan, Daniella Yeheskely-Hayon, Limor Minai, Eldad J Dann, Dvir Yelin
2012 Biomedical Optics Express  
Optical microscopy of blood cells in vivo provides a unique opportunity for clinicians and researchers to visualize the morphology and dynamics of circulating cells, but is usually limited by the imaging  ...  subcellular resolution imaging of red and white blood cells flowing in the oral mucosa of a human volunteer.  ...  SEFC images of flowing cells in a patient are compared to in vitro images and to fluorescence images of sorted blood cells for assisting in vivo data interpretation and establishing the clinical diagnostic  ... 
doi:10.1364/boe.3.001455 pmid:22741090 pmcid:PMC3370984 fatcat:e4to7ik2anejbboo5t7yveijgm

Imaging hemodynamics

Dominique Jennings, Natarajan Raghunand, Robert J. Gillies
2008 Cancer Metastasis Review  
This review discusses those imaging modalities, the advantages and disadvantages of each, and how they compare in terms of their ability to deliver information about therapy-associated changes in microvessel  ...  Morphology-A physiologically normal blood vessel is an unctuous conduit for blood flow. Both arteries and veins have three identifiable or distinct layers: tunica intima, media and adventitia [1] .  ...  Of course, a major limitation is that the cells must originate from the chicken (Gallus gallus). In Situ Assays: The advantage of the in situ assay is that it can be used in rabbits, rats and mice.  ... 
doi:10.1007/s10555-008-9157-4 pmid:18506397 pmcid:PMC3990228 fatcat:xrfutsw24jftrlgrh7wa3au4jq

Intravital Imaging

Mikael J. Pittet, Ralph Weissleder
2011 Cell  
In this Primer, we review the technologies that made this revolution possible and demonstrate how intravital imaging is beginning to provide quantitative and dynamic insights into cell biology, immunology  ...  Now, a new era of intravital fluorescence microscopy has dawned.  ...  Thomas Reiner, Sarah Earley, and Katy Yang for some of the examples in Figures 1 and 3; and Dr. Yvonna Fisher-Jeffes for review. Special thanks go to Drs.  ... 
doi:10.1016/j.cell.2011.11.004 pmid:22118457 pmcid:PMC3824153 fatcat:c75zhkri5bhirh3rk2xkuiw2yy

Intravital Imaging of Pulmonary Immune Response in Inflammation and Infection

Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
2021 Frontiers in Cell and Developmental Biology  
Intravital microscopy (IVM) is a unique imaging method providing insights in cellular functions and interactions in real-time, without the need for tissue extraction from the body.  ...  Various surgical approaches and microscopic setups have been adapted in order to overcome these challenges.  ...  All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.  ... 
doi:10.3389/fcell.2020.620471 pmid:33520993 pmcid:PMC7843704 fatcat:75r2qhpgtvcrnkxrwsga6xf3vq

Quantification of the endothelial surface glycocalyx on rat and mouse blood vessels

Wan-Yi Yen, Bin Cai, Min Zeng, John M. Tarbell, Bingmei M. Fu
2012 Microvascular Research  
To quantify the SGL thickness on the microvessels of rat mesentery and mouse cremaster muscle in situ, we applied a single vessel cannulation and perfusion technique to directly inject FITC-anti-heparan  ...  The glycocalyx on the surface of endothelium lining blood vessel walls modulates vascular barrier function, cell adhesion and also serves as a mechano-sensor for blood flow.  ...  Our study provides a new approach for in situ/in vivo measurement of the SGL thickness in blood vessels of various sizes from less than 10 μm to more than 2 mm diameter.  ... 
doi:10.1016/j.mvr.2012.02.005 pmid:22349291 pmcid:PMC3371771 fatcat:xs5nv6tmtbcnfongfbamnrjrmm

Recent Developments in Vascular Imaging Techniques in Tissue Engineering and Regenerative Medicine

Paul Kumar Upputuri, Kathyayini Sivasubramanian, Chong Seow Khoon Mark, Manojit Pramanik
2015 BioMed Research International  
Adequate vascularisation is key in determining the clinical outcome of stem cells and engineered tissue in regenerative medicine.  ...  We then summarize the strengths and challenges of these methods for preclinical and clinical applications.  ...  Figures 2(a) vivo imaging of blood flow in vessels (∼25 m in diameter) in a dorsal skinfold window chamber model was successfully demonstrated and compared with intravital fluorescence confocal microscopy  ... 
doi:10.1155/2015/783983 pmid:25821821 pmcid:PMC4363824 fatcat:356osf5utfccfp6szmzxotd6zq

Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins

Robert M. Hoffman
2008 Clinical and Experimental Metastasis  
Fluorescent proteins thus enable both macro and micro imaging technology and thereby provide the basis for the new field of in vivo cell biology.  ...  trafficking in both blood vessels and lymphatic vessels in the live animal.  ...  [33] have used GFP imaging to view cells in time-lapse images in a single optical section using a confocal microscope.  ... 
doi:10.1007/s10585-008-9205-z pmid:18787963 fatcat:zglvrn6abrdd5kmctpyzjtscry

Capillary perfusion of the rat brain cortex. An in vivo confocal microscopy study

A Villringer, A Them, U Lindauer, K Einhäupl, U Dirnagl
1994 Circulation Research  
Confocal laser-scanning microscopy was used to visualize subsurface cerebral microvessels labeled with intravascular fluorescein in a closed cranial window model of the anesthetized rat.  ...  Mean blood cell velocity was 498.3 ±443.9 ,um/s, and the mean blood cell supply rate was 35.75±28.01 cells per second.  ...  Acknowledgments This study was supported by the Sander-Stiftung (90.023.2) and the Deutsche Forschungsgemeinschaft (Di 454/4-2).  ... 
doi:10.1161/01.res.75.1.55 pmid:8013082 fatcat:ovzk6kzvbngbvgiqrqpx7upita

Intravital imaging of anti-tumor immune response and the tumor microenvironment

Tomasz Zal, Grzegorz Chodaczek
2010 Seminars in Immunopathology  
In the hands of cancer immunologists, intravital video microscopy is beginning to unravel the complexity of effector and suppressory lymphocytic interactions in tumors and in the draining lymphoid organs  ...  by minimally invasive observation using multi-photon and other low impact confocal microscopy techniques and fluorescent animal tumor models.  ...  Gascoigne for the CD8β-YFP mice, Xiao-Feng Qin for the MCA-mCerulean cells, and Janos Roszik for comments, and our families for their support.  ... 
doi:10.1007/s00281-010-0217-9 pmid:20652252 pmcid:PMC2971683 fatcat:62ty5rl7xfcb3hw63cvir2d3s4
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