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Development of a short-term,in vivomutagenesis assay: the effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice

Steven W. Kohler, G.Scott Provost, Patricia L. Kretz, Mark J. Dycaico, Joseph A. Sorge, Jay M. Short
1990 Nucleic Acids Research  
MATERIALS AND METHODS Transgenic mice B6CBAF1/J mice were obtained from the Jackson Laboratories (Bar Harbor, ME).  ...  Transgenic mice were prepared by injecting fertilized single cell embryos from B6CBAFI/J mice with approximately 200 copies of cos ligated lambda DNA, essentially as described by Hogan Table II  ... 
doi:10.1093/nar/18.10.3007 pmid:1693420 pmcid:PMC330831 fatcat:x4mh3tni5vfhvebahvrkevin6i

Genome-wide variation of the somatic mutation frequency in transgenic plants

I. Kovalchuk
2000 EMBO Journal  
The numbers on the top of the bars represent copy numbers of the transgenes; the transgenes in the sublines marked with an asterisk are integrated in complex patterns of single or multiple loci.  ...  The germline mutation frequency in transgenic rodents, as calculated for a single transgene copy, was~10 ±9 per base pair (Kohler et al., 1991; Dycaico et al., 1994) .  ... 
doi:10.1093/emboj/19.17.4431 pmid:10970837 pmcid:PMC302052 fatcat:qzuza4b7mrho3g7aih4nwcnxly

Epstein–Barr Virus/Human Vector Provides High-Level, Long-Term Expression of α1-Antitrypsin in Mice

Stephanie M Stoll, Christopher R Sclimenti, Edward J Baba, Leonard Meuse, Mark A Kay, Michele P Calos
2001 Molecular Therapy  
We constructed pEF-AAT by cloning a 19-kb SalI fragment from phage clone ␣NN (a gift from Mark Dycaico, Stratagene) carrying the complete human SERPINA1 into pEF.  ... 
doi:10.1006/mthe.2001.0429 pmid:11482983 fatcat:bkfrbsbh2vgz3juabhfwepzdcu

Transgenic Animal Models in Toxicology: Historical Perspectives and Future Outlook

Darrell R. Boverhof, Mark P Chamberlain, Clifford R. Elcombe, Frank J. Gonzalez, Robert H. Heflich, Lya G. Hernández, Abigail C. Jacobs, David Jacobson-Kram, Mirjam Luijten, Adriana Maggi, Mugimane G. Manjanatha, Jan van Benthem (+1 others)
2011 Toxicological Sciences  
(By J. Pendola in TRANSGENIC ANIMAL MODELS IN TOXICOLOGY 209 Downloaded from  ...  Subsequently, a lacI transgenic rat was developed in a Fischer 344 background (Dycaico et al., 1994) containing 30 copies of the shuttle vector per diploid genome.  ...  The transgene is present in approximately 40 copies per chromosome with integration occurring in a head-to-tail fashion at a single locus on chromosome 4 (Dycaico et al., 1994) .  ... 
doi:10.1093/toxsci/kfr075 pmid:21447610 fatcat:esy25m3apfej7h46h6wcelevpy

The Unfolded Protein Response Modulates Disease Severity in Pelizaeus-Merzbacher Disease

Cherie M Southwood, James Garbern, Wei Jiang, Alexander Gow
2002 Neuron  
Product identities were confirmed by size and restriction Allison, J., Campbell, I.L., Morahan, G., Mandel, T.E., Harrison, L.C., enzyme mapping.  ...  Debris surrounding the cell body marks the locations of cell processes likely re-spinal cord ‫%01ف(‬ of total cells).  ...  The interpretatations cause emphysema and missense mutations action from these data and additional mutations (A.G., cumulating in the ER cause hepatitis (Dycaico et al., unpublished data) is illustrated  ... 
doi:10.1016/s0896-6273(02)01045-0 pmid:12441049 pmcid:PMC4603660 fatcat:awjmuqhklfbdzjsh3mitvb7eky

The Endosomal Protein-Sorting Receptor Sortilin Has a Role in Trafficking -1 Antitrypsin

C. L. Gelling, I. W. Dawes, D. H. Perlmutter, E. A. Fisher, J. L. Brodsky
2012 Genetics  
In contrast, in the pep4D strain, there was a marked increase in higher-molecular-weight AT species over time.  ...  Live-cell fluorescence imaging of yeast expressing AT-GFP (pATM-2 or pATZ-2) and a Sec63-mCherry fusion (pBK177, obtained from J.  ... 
doi:10.1534/genetics.112.143487 pmid:22923381 pmcid:PMC3522165 fatcat:xtd2n36eq5bblfqc5p5x4fzrf4