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Engineering a Nickase on the Homing Endonuclease I-DmoI Scaffold

Rafael Molina, María José Marcaida, Pilar Redondo, Marco Marenchino, Phillippe Duchateau, Marco D'Abramo, Guillermo Montoya, Jesús Prieto
<span title="2015-06-04">2015</span> <i title="American Society for Biochemistry &amp; Molecular Biology (ASBMB)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/mryncoafc5cxdicldzfm4vlsze" style="color: black;">Journal of Biological Chemistry</a> </i> &nbsp;
The use of nickases may avoid toxicity associated with the NHEJ repair pathway in nuclease-induced gene targeting. Results: Decoupling the action of the I-DmoI catalytic residues acting on each strand generates a nickase. Conclusion: An I-DmoI variant was developed for cleaving one of the strands of its DNA target. Significance: This is the first structure of an engineered nickase in an LAGLIDADG scaffold. Homing endonucleases are useful tools for genome modification because of their capability
more &raquo; ... to recognize and cleave specifically large DNA targets. These endonucleases generate a DNA double strand break that can be repaired by the DNA damage response machinery. The break can be repaired by homologous recombination, an error-free mechanism, or by non-homologous end joining, a process susceptible to introducing errors in the repaired sequence. The type of DNA cleavage might alter the balance between these two alternatives. The use of "nickases" producing a specific single strand break instead of a double strand break could be an approach to reduce the toxicity associated with non-homologous end joining by promoting the use of homologous recombination to repair the cleavage of a single DNA break. Taking advantage of the sequential DNA cleavage mechanism of I-DmoI LAGLIDADG homing endonuclease, we have developed a new variant that is able to cut preferentially the coding DNA strand, generating a nicked DNA target. Our structural and biochemical analysis shows that by decoupling the action of the catalytic residues acting on each strand we can inhibit one of them while keeping the other functional.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1074/jbc.m115.658666">doi:10.1074/jbc.m115.658666</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/26045557">pmid:26045557</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC4513113/">pmcid:PMC4513113</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/zocyig5fc5auzlea5b6uukruvq">fatcat:zocyig5fc5auzlea5b6uukruvq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180725031224/http://www.jbc.org/content/290/30/18534.full.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/47/c2/47c2240a25246df9b1fadf1d6eda37380d7765a5.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1074/jbc.m115.658666"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513113" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Non-specific protein–DNA interactions control I-CreI target binding and cleavage

Rafael Molina, Pilar Redondo, Stefano Stella, Marco Marenchino, Marco D'Abramo, Francesco Luigi Gervasio, Jean Charles Epinat, Julien Valton, Silvestre Grizot, Phillipe Duchateau, Jesús Prieto, Guillermo Montoya
<span title="2012-04-11">2012</span> <i title="Oxford University Press (OUP)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/hfp6p6inqbdexbsu4r7usndpte" style="color: black;">Nucleic Acids Research</a> </i> &nbsp;
Homing endonucleases represent protein scaffolds that provide powerful tools for genome manipulation, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. The basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. Protein-DNA interaction engineering of homing endonucleases has demonstrated the potential of these approaches to create new specific instruments to target
more &raquo; ... genes for inactivation or repair. Protein-DNA interface studies have been focused mostly on specific contacts between amino acid side chains and bases to redesign the binding interface. However, it has been shown that 4 bp in the central DNA sequence of the 22-bp substrate of a homing endonuclease (I-CreI), which do not show specific protein-DNA interactions, is not devoid of content information. Here, we analyze the mechanism of target discrimination in this substrate region by the I-CreI protein, determining how it can occur independently of the specific protein-DNA interactions. Our data suggest the important role of indirect readout in this substrate region, opening the possibility for a fully rational search of new target sequences, thus improving the development of redesigned enzymes for therapeutic and biotechnological applications.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1093/nar/gks320">doi:10.1093/nar/gks320</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/22495931">pmid:22495931</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3413129/">pmcid:PMC3413129</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/5usca6yvlzcy7bqaa5wtmcl324">fatcat:5usca6yvlzcy7bqaa5wtmcl324</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20181030110243/https://watermark.silverchair.com/gks320.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAiowggImBgkqhkiG9w0BBwagggIXMIICEwIBADCCAgwGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMkP8UqSqb-DqsMwmLAgEQgIIB3aZfsDkJGhoVyPjg2ED19BAu8PJyFkvyHS08iIUcfq0-wRUQ6cU_VNFwejukhPNgIVILDczRdnJ7q9PkNLhFUR6wbe-SJiAvbpZCOa_ez1XmNJKepx2wdQ6NLhPS9j9TLGLPfGOi0wphbCpWhtJrW-zVbT89qelr7TEVAzDCh6V44FIUy1D4qXughxeUR7dhNLjkrd88C_QbvFD8dpxatRVXNijnVZHIuIc4hxn4v_a9nJ8NbVzWXTsbjcv071q_NDGPS_5A0iNL1Bn6UJge2RJ9uddMNfAGmyc-FY6Od0BMj7hMwIRsdwaOd3yBEtnKNnEdB_wj_-6NycmN4y1kTxGaMcTHcbdp8ET_fh--QkLWfAdKtzPZNRIVO4KM-mjwq4184GI22PJnbzA7BddYdnDZRtpvB7VCN6MIFiYkQVz2_YVcOspLxSoId8g11otUC0Xh2LdSBlgiv5ulsnu81hwbknD9H03NRDPMfHN879wWNMIqCKXG63IVsLi6yJMLr3oDCs9E7jeHCy3AAnQHgMePxEBdE9QmxM-EJHznRbbyvK3O8Cfk4pY_1VmSRpAF-8FJfp_rU_zM0oTTuTTPhS1-tUYSN0foniJwxQaVSAtGY-nQcT1khjKUtNA8Yg" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/30/3d/303d6d3314776297cfb2d73403dd6ae4f34d854b.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1093/nar/gks320"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> oup.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413129" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Rapid and efficient purification of RNA-binding proteins: Application to HIV-1 Rev

Marco Marenchino, David W. Armbruster, Mirko Hennig
<span title="">2009</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/wub26xua2zfvzgihx44vwainli" style="color: black;">Protein Expression and Purification</a> </i> &nbsp;
Non-specifically bound nucleic acid contaminants are an unwanted feature of recombinant RNAbinding proteins purified from Escherichia coli (E. coli). Removal of these contaminants represents an important step for the proteins' application in several biological assays and structural studies. The method described in this paper is a one-step protocol which is effective at removing tightly bound nucleic acids from over-expressed tagged HIV-1 Rev in E. coli. We combined affinity chromatography under
more &raquo; ... denaturing conditions with subsequent on-column refolding, to prevent selfassociation of Rev while removing the nucleic acid contaminants from the end product. We compare this purification method with an established, multi-step protocol involving precipitation with polyethyleneimine (PEI). As our tailored protocol requires only one step to simultaneously purify tagged proteins and eliminate bound cellular RNA and DNA, it represents a substantial advantage in time, effort, and expense.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.pep.2008.09.010">doi:10.1016/j.pep.2008.09.010</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/18852051">pmid:18852051</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC2624574/">pmcid:PMC2624574</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/h5nwhdredngoxo5xcud6b567ou">fatcat:h5nwhdredngoxo5xcud6b567ou</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200209204725/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2624574&amp;blobtype=pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/d3/fb/d3fb30340e84a47030726d1b7ab97bfc421f1a1f.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.pep.2008.09.010"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> elsevier.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2624574" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Maintenance of the Photovolatic Plants Using UAV Equipped with Low-cost GNSS RTK Receiver

Bilal Muhammad, Ramjee Prasad, Marco Nisi2, Fabio Menichetti2, Ernestina Cianca, Alberto Mennella, Graziano Gagliarde, Davide Marenchino
<span title="2018-02-27">2018</span> <i title="River Publishers"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/6wexo6vpdfdc7degct7c2chds4" style="color: black;">Journal of Mobile Multimedia</a> </i> &nbsp;
Marco Nisi has a master degree in "Aerospace Engineering" attained in "La Sapienza, Rome" and a post-graduate master's degree with highest honours in "Advanced Navigation and Communication Satellite Systems  ...  Davide Marenchino is a civil engineering, graduated at the Politecnico of Turin in 2004. He attained a Ph.D. in "Land and Environment" in Turin, working on researches in the geomatic field.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.13052/1550-4646.1412">doi:10.13052/1550-4646.1412</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/japvnrganbgdzkg75dn246omji">fatcat:japvnrganbgdzkg75dn246omji</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20210716173050/https://journals.riverpublishers.com/index.php/JMM/article/download/4429/3183" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/17/34/173400f7234517602b5baccb7f16f7fd45ebd0e7.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.13052/1550-4646.1412"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

Obtención de Chalconas, Catalizada por Óxidos Mixtos de Al-Mg Modificadas con Metales Básicos

Silvia N. Mendieta, Javier Fernandez-Marenchino, Marcos I. Oliva, Julio D. Fernández, Celso F. Perez, Mónica E. Crivello
<span title="">2018</span> <i title="Universidad Tecnologica Nacional"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/so5jv6rbqzevfayrkog34mwq24" style="color: black;">Revista Tecnología y Ciencia</a> </i> &nbsp;
reacción de Wittig para la síntesis de alquenos, por interacción de una sal de fosfonio con una cetona o un aldehído Revista Tecnología y Ciencia Universidad Tecnológica Nacional mendieta, fernandez-marenchino  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener" href="https://doaj.org/article/9ef37a3c5f54424b9da96f6a636f836f">doaj:9ef37a3c5f54424b9da96f6a636f836f</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/izlx72uc7ffghgil72of3ob3s4">fatcat:izlx72uc7ffghgil72of3ob3s4</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190806190101/http://rtyc.utn.edu.ar/index.php/rtyc/article/download/162/151/" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/9c/5a/9c5ab14518f9e91f54dc504ca47932f674749340.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a>

Maintenance of the Photovolatic PlantsUsing UAV Equipped with Low-costGNSS RTK Receiver

Bilal Muhammad, Ramjee Prasad, Marco Nisi, Fabio Menichetti, Ernestina Cianca, Alberto Mennella, Graziano Gagliarde, Davide Marenchino
<span title="">2018</span> <i title="River Publishers"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/felac6hlrvg3jdppwi6hk6bumm" style="color: black;">Journal of Communication Navigation Sensing and Services (CONASENSE)</a> </i> &nbsp;
Marco Nisi has a master degree in "Aerospace Engineering" attained in "La Sapienza, Rome" and a post-graduate master's degree with highest honours in "Advanced Navigation and Communication Satellite Systems  ...  Davide Marenchino is a civil engineering, graduated at the Politecnico of Turin in 2004. He attained a Ph.D. in "Land and Environment" in Turin, working on researches in the geomatic field.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.13052/jconasense2246-2120.2017.002">doi:10.13052/jconasense2246-2120.2017.002</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/6u35cehcynhdrpp52mjqubdjfi">fatcat:6u35cehcynhdrpp52mjqubdjfi</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20181030053835/https://pure.au.dk/ws/files/125437119/Maintenance_of_the_Photovolatic_Plants.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/6d/e6/6de665c8e716535ed9fbdb8ea048c465f89e2965.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.13052/jconasense2246-2120.2017.002"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

Dynamics and Cleavability at the α-Cleavage Site of APP(684-726) in Different Lipid Environments

Marco Marenchino, Philip T.F. Williamson, Samuel Murri, Giorgia Zandomeneghi, Heidi Wunderli-Allenspach, Beat H. Meier, Stefanie D. Krämer
<span title="">2008</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/dez2wau7t5aqvklzwrcsreuwq4" style="color: black;">Biophysical Journal</a> </i> &nbsp;
The occurrence of late-onset Alzheimer's disease has been related to the lipid homeostasis. We tested whether the membrane lipid environment affects the dynamics and cleavability of a model peptide corresponding to the amino acid sequence 684-726 of the amyloid precursor protein APP reconstituted in liposomes. Solid-state NMR with 2 H-Ala 713 , which is located within the putative transmembrane domain, suggested that the peptide observes less rotational motion in egg phosphatidylcholine (PhC)
more &raquo; ... mbranes than in dimyristoyl-phosphatidylcholine (DMPC) bilayers above the main phase transition temperature T c . The residue 15 N-Ala 692 , which is in the vicinity of the a-cleavage site, i.e., Lys 687 , showed less motion after reconstitution in distearoylphosphatidylcholine liposomes ,T c than in PhC, DMPC, or sphingomyelin vesicles. In all tested liposomal systems the a-cleavage site was accessible for hydrolysis by trypsin. However, the catalytic rate constant was higher in the PhC and DMPC than in the sphingomyelin and distearoyl-phosphatidylcholine systems. In conclusion, the dynamics of APP(684-726) on the transmembrane level as well as the motion of the a-cleavage site and its hydrolysis by a model enzyme are dependent on the bilayer characteristics. This could be relevant for the processing of APP in vivo.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1529/biophysj.108.129726">doi:10.1529/biophysj.108.129726</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/18390599">pmid:18390599</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC2479613/">pmcid:PMC2479613</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/27f6dtulrfc4dfz7mfapwzeo3u">fatcat:27f6dtulrfc4dfz7mfapwzeo3u</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190312190848/https://core.ac.uk/download/pdf/82455418.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/43/83/4383632fc0ea5364bbbbd739790de0360fab2c80.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1529/biophysj.108.129726"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2479613" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Inés G. Muñoz, Jesús Prieto, Sunita Subramanian, Javier Coloma, Pilar Redondo, Maider Villate, Nekane Merino, Marco Marenchino, Marco D'Abramo, Francesco L. Gervasio, Sylvestre Grizot, Fayza Daboussi (+9 others)
<span title="2010-09-16">2010</span> <i title="Oxford University Press (OUP)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/hfp6p6inqbdexbsu4r7usndpte" style="color: black;">Nucleic Acids Research</a> </i> &nbsp;
Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in RAG1 produce severe combined immunodeficiency (SCID), a monogenic disease leading to defective immune response in the individuals, leaving them vulnerable to infectious diseases.
more &raquo; ... e structures of two engineered heterodimeric variants and one single-chain variant of I-CreI, in complex with a 24-bp oligonucleotide of the human RAG1 gene sequence, show how the DNA binding is achieved through interactions in the major groove. In addition, the introduction of the G19S mutation in the neighborhood of the catalytic site lowers the reaction energy barrier for DNA cleavage without compromising DNA recognition. Gene-targeting experiments in human cell lines show that the designed single-chain molecule preserves its in vivo activity with higher specificity, further enhanced by the G19S mutation. This is the first time that an engineered meganuclease variant targets the human RAG1 locus by stimulating homologous recombination in human cell lines up to 265 bp away from the cleavage site. Our analysis illustrates the key features for à la carte procedure in protein-DNA recognition design, opening new possibilities for SCID patients whose illness can be treated ex vivo.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1093/nar/gkq801">doi:10.1093/nar/gkq801</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/20846960">pmid:20846960</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3025557/">pmcid:PMC3025557</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/yspb3htegvbifc4xbggebvtu7y">fatcat:yspb3htegvbifc4xbggebvtu7y</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190226075843/http://pdfs.semanticscholar.org/6e6e/ada317bfbcd024bc57b9658a01064f229b26.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/6e/6e/6e6eada317bfbcd024bc57b9658a01064f229b26.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1093/nar/gkq801"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> oup.com </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025557" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

The Conformational Stability and Biophysical Properties of the Eukaryotic Thioredoxins of Pisum Sativum Are Not Family-Conserved

David Aguado-Llera, Ana Isabel Martínez-Gómez, Jesús Prieto, Marco Marenchino, José Angel Traverso, Javier Gómez, Ana Chueca, José L. Neira, Annalisa Pastore
<span title="2011-02-22">2011</span> <i title="Public Library of Science (PLoS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/s3gm7274mfe6fcs7e3jterqlri" style="color: black;">PLoS ONE</a> </i> &nbsp;
Citation: Aguado-Llera D, Martínez-Gó mez AI, Prieto J, Marenchino M, Traverso JA, et al. (2011) The Conformational Stability and Biophysical Properties of the Eukaryotic Thioredoxins of Pisum Sativum  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1371/journal.pone.0017068">doi:10.1371/journal.pone.0017068</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/21364950">pmid:21364950</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3043092/">pmcid:PMC3043092</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/hmx27ztimvcwno2mskkkg5vboa">fatcat:hmx27ztimvcwno2mskkkg5vboa</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170928003632/http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017068&amp;type=printable" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/32/76/3276af5e7d4e5470d660d4bf3a9ae1e9b8a599d8.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1371/journal.pone.0017068"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> plos.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043092" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Conformational Selection and Induced Fit Mechanisms in the Binding of an Anticancer Drug to the c-Src Kinase

Maria Agnese Morando, Giorgio Saladino, Nicola D'Amelio, Encarna Pucheta-Martinez, Silvia Lovera, Moreno Lelli, Blanca López-Méndez, Marco Marenchino, Ramón Campos-Olivas, Francesco Luigi Gervasio
<span title="2016-04-18">2016</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tnqhc2x2aneavcd3gx5h7mswhm" style="color: black;">Scientific Reports</a> </i> &nbsp;
Understanding the conformational changes associated with the binding of small ligands to their biological targets is a fascinating and meaningful question in chemistry, biology and drug discovery. One of the most studied and important is the so-called "DFG-flip" of tyrosine kinases. The conserved three amino-acid DFG motif undergoes an "in to out" movement resulting in a particular inactive conformation to which "type II" kinase inhibitors, such as the anti-cancer drug Imatinib, bind. Despite
more &raquo; ... ny studies, the details of this prototypical conformational change are still debated. Here we combine various NMR experiments and surface plasmon resonance with enhanced sampling molecular dynamics simulations to shed light into the conformational dynamics associated with the binding of Imatinib to the proto-oncogene c-Src. We find that both conformational selection and induced fit play a role in the binding mechanism, reconciling opposing views held in the literature. Moreover, an external binding pose and local unfolding (cracking) of the aG helix are observed.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/srep24439">doi:10.1038/srep24439</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/27087366">pmid:27087366</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC4834493/">pmcid:PMC4834493</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/v4aes4372jeqnidwgopn5dk6ta">fatcat:v4aes4372jeqnidwgopn5dk6ta</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20180719020534/http://discovery.ucl.ac.uk/1486063/1/Conformational%20Selection%20and%20Induced%20Fit%20Mechanisms%20in%20the%20Binding%20of%20an%20Anticancer%20Drug%20to%20the%20c-Src%20Kinase.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/eb/dd/ebdd5f133abb3c8007bb3945e88097daaa9a311a.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1038/srep24439"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> Publisher / doi.org </button> </a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834493" title="pubmed link"> <button class="ui compact blue labeled icon button serp-button"> <i class="file alternate outline icon"></i> pubmed.gov </button> </a>

Peripheral Blood CD34+ Percentage at Hematological Recovery after Chemotherapy Is a Good Early Predictor of Harvest: A Single-Center Experience

Roberto Sorasio, Margherita Bonferroni, Mariella Grasso, Giuliana Strola, Davide Rapezzi, Dario Marenchino, Cristina Di Marco, Claudia Castellino, Daniele Mattei, Nicola Mordini, Francesca Fiore, Ivana Celeghini (+3 others)
<span title="">2014</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/2kpnxw7dl5b43oarayv3jpkf7m" style="color: black;">Biology of Blood and Marrow Transplantation</a> </i> &nbsp;
Several algorithms for early prediction of poor-mobilizing patients after chemotherapy and granulocyte colony-stimulating factor administration have been proposed. They generally define peripheral blood cut-off levels of CD34þ cells at a fixed day after starting chemotherapy, mostly with cyclophosphamide. To define an algorithm for early addition of plerixafor regardless of the chemotherapy regimen used, we retrospectively analyzed 280 chemomobilization attempts in 236 patients treated at our
more &raquo; ... stitution between 2002 and 2012. In multivariate analysis, CD34þ absolute count and CD34þ percentage upon total leukocyte count at day 1 (defined as the first day in which leukocytes reached a value > 1 Â 10 9 /L) were the only factors able to predict a total harvest ! 2 Â 10 6 CD34þ/kg. In patients with day 1 CD34þ lower than 20/mL, the CD34þ percentage was a more reliable predictor of stem cell harvest in the following days than CD34þ absolute count. Upon definition of the best CD34þ cut-off value for identification of poor-mobilizing patients, an algorithm was set up to guide plerixafor administration. It was prospectively validated in 20 patients in 2013 with encouraging results in terms of low incidences of both mobilization failure and plerixafor use. Large prospective trials that define the most cost-effective strategy for just-in-time rescue plerixafor are warranted. Ó
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bbmt.2014.02.002">doi:10.1016/j.bbmt.2014.02.002</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/24525280">pmid:24525280</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/v56wqspes5a5rf2kcno7jfoomq">fatcat:v56wqspes5a5rf2kcno7jfoomq</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190307052737/https://core.ac.uk/download/pdf/82542502.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/2c/60/2c60d31af061dd76ecfe6b3932890b599f1ee1b6.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.bbmt.2014.02.002"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> elsevier.com </button> </a>

LOEWE Workshop Particle Characterization in Medicine and Biology

<span title="">2018</span> <i title="Hapres"> Pharmaceutical Frontiers </i> &nbsp;
The event was organized by Marco Marenchino (Malvern Panalytical Ltd.) and Matthias G. Wacker (Fraunhofer IME/Goethe University).  ...  Marco Marenchino (Malvern Panalytical Ltd.). Figure 2 . 2 Photograph of the speakers involved in the workshop event at University hospital, Goethe University.  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.20900/pf20190002">doi:10.20900/pf20190002</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/uh7cfnj4f5cglc3krqtuhixcwa">fatcat:uh7cfnj4f5cglc3krqtuhixcwa</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190429070142/https://pf.hapres.com/UpLoad/PdfFile/PF_985.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/3b/c1/3bc14034c85fe80cb7b9cc3e31d6ede3a015ac88.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.20900/pf20190002"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> Publisher / doi.org </button> </a>

Solvent recovery from soybean oil/n-hexane mixtures using hollow fiber membrane

M. V. Tres, R. Nobrega, R. B. Carvalho, J. V. Oliveira, M. Di Luccio
<span title="">2012</span> <i title="FapUNIFESP (SciELO)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bzxeg7epjra37dg777dq4isvpq" style="color: black;">Brazilian Journal of Chemical Engineering</a> </i> &nbsp;
possible thermal degradation of oil and an incomplete elimination of n-hexane are major disadvantages of this technology, besides the large amount of energy used in these processing steps (Reverchon and De Marco  ...  Organic solvents are used to improve the polarity of the membrane (the membrane becoming more polar or nonpolar) (Marenchino et al., 2006) or to prevent the closing of the pores present in some membranes  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1590/s0104-66322012000300015">doi:10.1590/s0104-66322012000300015</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/ebnjznhez5dbbem2rvwf32qypm">fatcat:ebnjznhez5dbbem2rvwf32qypm</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170818031313/http://www.scielo.br/pdf/bjce/v29n3/15.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/2a/ed/2aedad4fb6197e9906ffdfe368035c570d170726.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1590/s0104-66322012000300015"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> scielo.br </button> </a>

Solvent recovery from soybean oil/n-butane mixtures using a hollow fiber ultrafiltration membrane

M. V. Tres, J. C. Racoski, R. Nobrega, R. B. Carvalho, J. V. Oliveira, M. Di Luccio
<span title="">2014</span> <i title="FapUNIFESP (SciELO)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/bzxeg7epjra37dg777dq4isvpq" style="color: black;">Brazilian Journal of Chemical Engineering</a> </i> &nbsp;
thermal degradation of oil and an incomplete elimination of n-hexane are the major disadvantages of this technology, besides the large amount of energy used in these processing steps (Reverchon and De Marco  ...  Organic solvents can be used to improve the polarity of the membrane (the membrane becomes more polar or nonpolar) (Marenchino et al., 2006) or to prevent pore closing in some membranes when they are  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1590/s0104-66322014000100022">doi:10.1590/s0104-66322014000100022</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/pkpwrqohona4peic4c63lvbkp4">fatcat:pkpwrqohona4peic4c63lvbkp4</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170705222113/http://www.scielo.br/pdf/bjce/v31n1/22.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/63/ba/63ba7d219cb1248216a31f228741e8f4d6436555.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1590/s0104-66322014000100022"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="unlock alternate icon" style="background-color: #fb971f;"></i> scielo.br </button> </a>

Evaluation of permeation of macauba oil and n-hexane mixtures through polymeric commercial membranes subjected to different pre-treatments

Frederico Marques Penha, Katia Rezzadori, Mariane Carolina Proner, Guilherme Zin, Lara Alexandre Fogaça, José Carlos Cunha Petrus, J. Vladimir de Oliveira, Marco Di Luccio
<span title="">2015</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ms7lui5rlvernkzlua3pwy773m" style="color: black;">Journal of Food Engineering</a> </i> &nbsp;
Other hypothesis, pointed out by Marenchino et al. (2006) , takes into account the hydrophilic nature of the membrane and presumes that the flux reduction behaviour would be caused by the presence of  ...  Marenchino et al. (2006) , Pagliero et al. (2011) and Firman et al. (2013) reported that the increase of the process temperature reduces the viscosity of the mixtures and consequently promotes gains  ... 
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.jfoodeng.2015.01.020">doi:10.1016/j.jfoodeng.2015.01.020</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/wb45m2et2jcsnkupiyaxzpqzee">fatcat:wb45m2et2jcsnkupiyaxzpqzee</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190320074527/https://core.ac.uk/download/pdf/82821536.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/64/ad/64ad7c9b6e136e4cb39a39b7e6ba9796e3864cfb.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.jfoodeng.2015.01.020"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> elsevier.com </button> </a>
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