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Olfactory Ensheathing Cells: Characteristics, Genetic Engineering, and Therapeutic Potential

Marc J. Ruitenberg, Jana Vukovic, Julijana Sarich, Samantha J. Busfield, Giles W. Plant
2006 Journal of Neurotrauma  
J. Neurosci. 24, 9799-9810. FIELD, P., LI, Y., and RAISMAN, G. (2003). Ensheathment of the olfactory nerves in the adult rat. J. Neurocytol. 32, 317-324.  ...  BLITS, B., DIJKHUIZEN, P.A., BOER, G.J., and VERHAA-GEN, J. (2000).  ... 
doi:10.1089/neu.2006.23.468 pmid:16629630 fatcat:tuwx5wcwhjbqxijbc7lxdn3tci

Traumatic Spinal Cord Injury and the Gut Microbiota: Current Insights and Future Challenges

Trisha Jogia, Marc J. Ruitenberg
2020 Frontiers in Immunology  
Copyright © 2020 Jogia and Ruitenberg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).  ... 
doi:10.3389/fimmu.2020.00704 pmid:32528463 pmcid:PMC7247863 fatcat:ox2uy5xsgfdndac32qpz7ipjwm

Promoting central nervous system regeneration: lessons from cranial nerve I

Marc J Ruitenberg, Jana Vukovic
2008 Restorative Neurology and Neuroscience  
Acknowledgements Marc Ruitenberg is a postdoctoral fellow supported by the Australian Research Council (Grant No. DP0774113). The authors would like to thank their colleague Prof. Alan R.  ...  J., & Cowan, W.  ...  ., 2003; Ruitenberg et al., 2004) .  ... 
pmid:18820410 fatcat:32svq3fvkjg6hkybvynwwvptbu

Complement activation in the injured central nervous system: another dual-edged sword?

Faith H Brennan, Aileen J Anderson, Stephen M Taylor, Trent M Woodruff, Marc J Ruitenberg
2012 Journal of Neuroinflammation  
The complement system, a major component of the innate immune system, is becoming increasingly recognised as a key participant in physiology and disease. The awareness that immunological mediators support various aspects of both normal central nervous system (CNS) function and pathology has led to a renaissance of complement research in neuroscience. Various studies have revealed particularly novel findings on the wideranging involvement of complement in neural development, synapse elimination
more » ... nd maturation of neural networks, as well as the progression of pathology in a range of chronic neurodegenerative disorders, and more recently, neurotraumatic events, where rapid disruption of neuronal homeostasis potently triggers complement activation. The purpose of this review is to summarise recent findings on complement activation and acquired brain or spinal cord injury, i.e. ischaemic-reperfusion injury or stroke, traumatic brain injury (TBI) and spinal cord injury (SCI), highlighting the potential for complement-targeted therapeutics to alleviate the devastating consequences of these neurological conditions.
doi:10.1186/1742-2094-9-137 pmid:22721265 pmcid:PMC3464784 fatcat:7a4iut25gffa7olsj2cpn7es64

Systemic Immune Response to Traumatic CNS Injuries—Are Extracellular Vesicles the Missing Link?

Abi G. Yates, Daniel C. Anthony, Marc J. Ruitenberg, Yvonne Couch
2019 Frontiers in Immunology  
Copyright © 2019 Yates, Anthony, Ruitenberg and Couch. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).  ... 
doi:10.3389/fimmu.2019.02723 pmid:31824504 pmcid:PMC6879545 fatcat:7rr5i3u5yzgmjode37dd4mqxe4

Vertebral landmarks for the identification of spinal cord segments in the mouse

Megan Harrison, Aine O'Brien, Lucy Adams, Gary Cowin, Marc J. Ruitenberg, Gulgun Sengul, Charles Watson
2013 NeuroImage  
Accurate identification of spinal cord segments in relation to vertebral landmarks is essential to surgery aimed at experimental spinal cord injury. We have analyzed a complete series of high-resolution magnetic resonance (MR) images from the mouse spine in order to delineate the boundaries of spinal cord segments in relation to vertebral landmarks. The resulting atlas can be used to plan experimental approaches that require the accurate identification of a target spinal cord segment.
doi:10.1016/j.neuroimage.2012.11.048 pmid:23246856 fatcat:tpm65m45knhgtpo5uxe2ykug64

Prognostic value of early leukocyte fluctuations for recovery from traumatic spinal cord injury [article]

Trisha Jogia, Tom Lübstorf, Esther Jacobson, Elissa Scriven, Sridhar Atresh, Thomas Liebscher, Jan S Schwab, Marcel A Kopp, James Walsham, Kate E Campbell, Marc J Ruitenberg
2020 biorxiv/medrxiv   pre-print
Marc J.  ...  Patients with cervical injury (B and J) and those that were intubated/mechanically ventilated (C and K) were more likely to acquire an airway infection (Fisher's and χ 2 test for B-C and J-K, respectively  ... 
doi:10.1101/2020.10.26.20220236 fatcat:6sipf2jibncazoo67qtd6k2yaa

Disease-modifying effect of intravenous immunoglobulin in an experimental model of epilepsy

Min Chen, Thiruma V. Arumugam, Gayeshika Leanage, Quang M. Tieng, Ashwin Yadav, Jeremy F. P. Ullmann, David T. She, Vy Truong, Marc J. Ruitenberg, David C. Reutens
2017 Scientific Reports  
Novel therapies that prevent or modify the development of epilepsy following an initiating brain insult could significantly reduce the burden of this disease. In light of evidence that immune mechanisms play an important role in generating and maintaining the epileptic condition, we evaluated the effect of a well-established immunomodulatory treatment, intravenous immunoglobulin (IVIg), on the development of epilepsy in an experimental model of epileptogenesis. In separate experiments, IVIg was
more » ... administered either before (pre-treatment) or after (post-treatment) the onset of pilocarpine status epilepticus (SE). Our results show that both pre-and post-treatment with IVIg attenuated acute inflammation in the SE model. Specifically, IVIg reduced local activation of glial cells, complement system activation, and blood-brain barrier damage (BBB), which are all thought to play important roles in the development of epilepsy. Importantly, post-treatment with IVIg was also found to reduce the frequency and duration of subsequent spontaneous recurrent seizures as detected by chronic videoelectroencephalographic (video-EEG) recordings. This finding supports a novel application for IVIg, specifically its repurposing as a disease-modifying therapy in epilepsy.
doi:10.1038/srep40528 pmid:28074934 pmcid:PMC5225452 fatcat:ahqdouboxvby5m74h5cvruvciu

Acute IL-1RA treatment suppresses the peripheral and central inflammatory response to spinal cord injury

Abi G. Yates, Trisha Jogia, Ellen R. Gillespie, Yvonne Couch, Marc J. Ruitenberg, Daniel C. Anthony
2021 Journal of Neuroinflammation  
Background The acute phase response (APR) to CNS insults contributes to the overall magnitude and nature of the systemic inflammatory response. Aspects of this response are thought to drive secondary inflammatory pathology at the lesion site, and suppression of the APR can therefore afford some neuroprotection. In this study, we examined the APR in a mouse model of traumatic spinal cord injury (SCI), along with its relationship to neutrophil recruitment during the immediate aftermath of the
more » ... lt. We specifically investigated the effect of IL-1 receptor antagonist (IL-1RA) administration on the APR and leukocyte recruitment to the injured spinal cord. Methods Adult female C57BL/6 mice underwent either a 70kD contusive SCI, or sham surgery, and tissue was collected at 2, 6, 12, and 24 hours post-operation. For IL-1RA experiments, SCI mice received two intraperitoneal injections of human IL-1RA (100mg/kg), or saline as control, immediately following, and 5 hours after impact, and animals were sacrificed 6 hours later. Blood, spleen, liver and spinal cord were collected to study markers of central and peripheral inflammation by flow cytometry, immunohistochemistry and qPCR. Results were analysed by two-way ANOVA or student's t-test, as appropriate. Results SCI induced a robust APR, hallmarked by elevated hepatic expression of pro-inflammatory marker genes and a significantly increased neutrophil presence in the blood, liver and spleen of these animals, as early as 2 hours after injury. This peripheral response preceded significant neutrophil infiltration of the spinal cord, which peaked 24 hours post-SCI. Although expression of IL-1RA was also induced in the liver following SCI, its response was delayed compared to IL-1β. Exogenous administration of IL-1RA during this putative therapeutic window was able to suppress the hepatic APR, as evidenced by a reduction in CXCL1 and SAA-2 expression as well as a significant decrease in neutrophil infiltration in both the liver and the injured spinal cord itself. Conclusions Our data indicate that peripheral administration of IL-1RA can attenuate the APR which in turn reduces immune cell infiltration at the spinal cord lesion site. We propose IL-1RA treatment as a viable therapeutic strategy to minimise the harmful effects of SCI-induced inflammation.
doi:10.1186/s12974-020-02050-6 pmid:33407641 fatcat:x7xt6ugh6vb6jhnb5kmcyrck6q

Immune cell trafficking from the brain maintains CNS immune tolerance

Mohammad G. Mohammad, Vicky W.W. Tsai, Marc J. Ruitenberg, Masoud Hassanpour, Hui Li, Prue H. Hart, Samuel N. Breit, Paul E. Sawchenko, David A. Brown
2014 Journal of Clinical Investigation  
In the CNS, no pathway dedicated to immune surveillance has been characterized for preventing the anti-CNS immune responses that develop in autoimmune neuroinflammatory disease. Here, we identified a pathway for immune cells to traffic from the brain that is associated with the rostral migratory stream (RMS), which is a forebrain source of newly generated neurons. Evaluation of fluorescently labeled leukocyte migration in mice revealed that DCs travel via the RMS from the CNS to the cervical
more » ... (CxLNs), where they present antigen to T cells. Pharmacologic interruption of immune cell traffic with the mononuclear cell-sequestering drug fingolimod influenced anti-CNS T cell responses in the CxLNs and modulated experimental autoimmune encephalomyelitis (EAE) severity in a mouse model of multiple sclerosis (MS). Fingolimod treatment also induced EAE in a disease-resistant transgenic mouse strain by altering DC-mediated Treg functions in CxLNs and disrupting CNS immune tolerance. These data describe an immune cell pathway that originates in the CNS and is capable of dampening anti-CNS immune responses in the periphery. Furthermore, these data provide insight into how fingolimod treatment might exacerbate CNS neuroinflammation in some cases and suggest that focal therapeutic interventions, outside the CNS have the potential to selectively modify anti-CNS immunity. Results CD11c + cells are associated with the rostral migratory stream (RMS). APCs are present in the choroid plexus and the meninges (22); however, their localization to the brain parenchyma in health is disputed
doi:10.1172/jci71544 pmid:24569378 pmcid:PMC3934177 fatcat:jle7oq7a2bewlngn27m5siybta

The Alternative Receptor for Complement Component 5a, C5aR2, Conveys Neuroprotection in Traumatic Spinal Cord Injury

Patrick J.C. Biggins, Faith H. Brennan, Stephen M. Taylor, Trent M. Woodruff, Marc J. Ruitenberg
2017 Journal of Neurotrauma  
more than 10 generations onto a C57BL6/J genetic background.  ...  All experimental mice were obtained from breeding colonies at The University of Queensland Biological Resources facility (Brisbane, Australia) (C5ar2 -/and C57BL6/J).  ...  Blood plasma analysis showed that C5ar2 -/mice also had significantly lower circulating IL12p70 levels (H), but not TNF (I), IL-6 (J), IFN-c (K), MCP-1 (L), and IL-10 (M).  ... 
doi:10.1089/neu.2016.4701 pmid:28173736 fatcat:uv4o23tibzggjaoaxlja4sbhuu

Absence of the C5a Receptor C5aR2 Worsens Ischemic Tissue Injury by Increasing C5aR1-Mediated Neutrophil Infiltration

Mike C. L. Wu, John D. Lee, Marc J. Ruitenberg, Trent M. Woodruff
2020 Journal of Immunology  
Intestinal villus neutrophil counts with esterase stain (J) confirmed elevated neutrophil numbers in C5aR2 2/2 mice compared with WT mice after IR injury.  ...  injury (7) and the dampening effect of C5aR2 on C5a-induced neutrophil chemotaxis (18) , a greater presence of these cells was observed in C5aR2 2/2 mice with IR compared with WT controls (Fig. 2F-J  ... 
doi:10.4049/jimmunol.2000778 pmid:33028618 fatcat:56nnupivg5frhd47ilewh5l4iq

IVIg attenuates complement and improves spinal cord injury outcomes in mice

Faith H. Brennan, Nyoman D. Kurniawan, Jana Vukovic, Perry F. Bartlett, Fabian Käsermann, Thiruma V. Arumugam, Milan Basta, Marc J. Ruitenberg
2016 Annals of Clinical and Translational Neurology  
A total of 99 wild-type (WT) C57BL6/J and 9 Emx1-creERT2:Rosa26-tdTomato mice on a C57BL6/J background were used in this study.  ...  which was again confirmed by "area under the curve" analysis (J).  ... 
doi:10.1002/acn3.318 pmid:27386499 pmcid:PMC4931715 fatcat:kszmifmjozeqzlx5pvks4sldxq

The Chemokine Receptor CX3CR1 Mediates Homing of MHC class II-Positive Cells to the Normal Mouse Corneal Epithelium

Holly R. Chinnery, Marc J. Ruitenberg, Giles W. Plant, Eric Pearlman, Steffen Jung, Paul G. McMenamin
2007 Investigative Ophthalmology and Visual Science  
FIGURE 3 . 3 Confocal microscopic analysis of corneal wholemounts from naïve WT (A), CX 3 CR1 ϩ/GFP (B, I), and CX 3 CR1 GFP/GFP (C, D-H, J-O) mice.  ...  Similar observations in our laboratory suggest that the same phenomena may also be true of monocyte-derived cells in the connective tissues of the respiratory mucosa (Ruitenberg M, unpublished data, 2006  ... 
doi:10.1167/iovs.06-0746 pmid:17389486 pmcid:PMC3392181 fatcat:uhz2tpbwbrallfhmxy3co5s53u

Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model

Jana Vukovic, Giles W. Plant, Marc J. Ruitenberg, Alan R. Harvey
2007 Neuron Glia Biology  
j. ruitenberg and alan r. harvey We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia (OEG) influence the specificity of axon-target cell interactions  ...  Influence of adult Schwann cells and olfactory ensheathing glia on axon-target cell interactions in the CNS: a comparative analysis using a retinotectal co-graft model jana vukovic giles w. plant marc  ...  Scale bars: A-D,G,H,J,K, 100 mm; E,F,I, 50 mm; L,M, 40 mm. Fig. 3 . 3 Quantitative analysis of cell survival and graft volume.  ... 
doi:10.1017/s1740925x07000671 pmid:18634570 fatcat:jbjq5xno4ja7lg4hsrsy237mju
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